A potassium channel protein encoded by chlorella virus PBCV-1

Science

Volumn 287, Issue 5458, 2000, Pages 1641-1644

  Plugge, B ^a   Gazzarrini, S ^b   Nelson, M ^c   Cerana, R ^d   Van Etten, J L ^e   Derst, C ^f   DiFrancesco, D ^b   Moroni, A ^b   Thiel, G ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b UNIVERSITY OF MILAN   (Italy)

^c Megabase Research Products   (United States)

^d UNIVERSITY OF MILANO BICOCCA   (Italy)

^e UNIVERSITY OF NEBRASKA LINCOLN   (United States)

^f PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

AMANTADINE; BARIUM; CESIUM; DOUBLE STRANDED DNA; MESSENGER RNA; POTASSIUM CHANNEL; PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CHLORELLA VIRUS; LIFE CYCLE; NONHUMAN; OPEN READING FRAME; PHYLOGENY; PLANT VIRUS; POTASSIUM CONDUCTANCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; VIRUS PLAQUE; XENOPUS LAEVIS;

AMANTADINE; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; BARIUM; CESIUM; CHLORELLA; ISOELECTRIC POINT; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; OOCYTES; PATCH-CLAMP TECHNIQUES; PHYCODNAVIRIDAE; PLAQUE ASSAY; POTASSIUM; POTASSIUM CHANNELS; RECOMBINANT PROTEINS; RNA, MESSENGER; SODIUM; VIRAL PROTEINS; VIRUS REPLICATION; XENOPUS LAEVIS;

ANIMALIA; CHLORELLA VIRUS; DNA VIRUSES; PARAMECIUM BURSARIA CHLORELLA VIRUS 1; XENOPUS LAEVIS;

EID: 0034008733     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.287.5458.1641     Document Type: Article

References (32)

1. L. Y. Jan and Y. N. Jan, Annu. Rev. Neurosci. 20, 91 (1997).
2. L. Heginbotham, Z. Lu, T. Abramson, R. MacKinnon, Biophys. J. 66, 1061 (1994).
3. Single-letter abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr. X indicates any residue.
4. R. MacKinnon, Nature 350, 232 (1991).
5. J. L. Van Etten and R. H. Meints, Annu. Rev. Microbiol. 53, 447 (1999).
6. D. A. Doyle et al., Science 280, 69 (1998).
7. C. Derst and A. Karschin, J. Exp. Biol. 201, 2791 (1998).
8. 2, and 5 mM Hepes/LiOH (pH 7.4). Mannitol was used to adjust the osmolarity to 215 mOsmol.
9. The amount of mRNA injected was limited to 1 ng/50 nl. Injection of higher amounts of mRNA or expression times longer than 48 hours caused oocytes to die.
10. - current [C. K. T. Bauer, J. R. Falk, W. Schwarz, Pfluegers Arch. 432, 812 (1996); N. Dascal, Crit. Rev. Biochem. 22, 317 (1987)].
11. - current [C. K. T. Bauer, J. R. Falk, W. Schwarz, Pfluegers Arch. 432, 812 (1996); N. Dascal, Crit. Rev. Biochem. 22, 317 (1987)].
12. 2O: +0.2 ± 0.05 μA and -0.22 ± 0.08 μA (n = 9); (ii) Kcv mRNA: + 1.63 ± 0.37 μA and -1.89 ± 0.44 μA (n = 17).
13. + concentrations were assumed to be 20 mM [K. Kusano, R. Miledi, J. Stinnakre, J. Physiol (London) 328, 143 (1982)] and 101 mM (based on the fitting of the Nernst plot in Fig. 2E), respectively.
14. + concentrations were assumed to be 20 mM [K. Kusano, R. Miledi, J. Stinnakre, J. Physiol (London) 328, 143 (1982)] and 101 mM (based on the fitting of the Nernst plot in Fig. 2E), respectively.
15. +-selective current in KcvF66A-expressing oocytes confirms that Kcv functions as a channel protein in oocytes and that the observed currents are not due to activation of endogenous channel proteins.
16. G. Yellen, Curr. Opin. Neurobiol. 9, 267 (1999); D. M. Papazian, Neuron 23, 7 (1999).
17. G. Yellen, Curr. Opin. Neurobiol. 9, 267 (1999); D. M. Papazian, Neuron 23, 7 (1999).
18. C. Wang, K. Takeuchi, L. H. Pinto, R. A. Lamb, J. Virol. 67, 5585 (1993).
19. 37 [C. S. Gandhi et al. J. Biol. Chem. 274, 5474 (1999)].
20. o = 660 ± 12 μM (n = 3 oocytes).
21. R. Z. Sabirov, T. Tominaga, A. Miwa, Y. Okada, S. Oiki, J. Gen. Physiol. 110, 665 (1997); R. C. Shieh, J. C. Chang, J. Arreola, Biophys. J. 75, 2313 (1993); J. M. Owen, C. C. Quinn, R. Leach, J. B. Findlay, M. R. Boyett, Exp. Physiol. 84, 471 (1999).
22. R. Z. Sabirov, T. Tominaga, A. Miwa, Y. Okada, S. Oiki, J. Gen. Physiol. 110, 665 (1997); R. C. Shieh, J. C. Chang, J. Arreola, Biophys. J. 75, 2313 (1993); J. M. Owen, C. C. Quinn, R. Leach, J. B. Findlay, M. R. Boyett, Exp. Physiol. 84, 471 (1999).
23. R. Z. Sabirov, T. Tominaga, A. Miwa, Y. Okada, S. Oiki, J. Gen. Physiol. 110, 665 (1997); R. C. Shieh, J. C. Chang, J. Arreola, Biophys. J. 75, 2313 (1993); J. M. Owen, C. C. Quinn, R. Leach, J. B. Findlay, M. R. Boyett, Exp. Physiol. 84, 471 (1999).
24. J. L. Van Etten, D. E. Burbank, Y. Xia, R. H. Meints, Virology 126, 117 (1983).
25. 8 host cells (Chlorella strain NC64A) and warm MBBM top agar containing various concentrations of ion-channel inhibitors. The mixture was layered onto MBBM nutrient agar (22). After incubating for 2 days at 25°C, viral plaques were counted. Potassium concentration of MBBM growth medium was 1.3 mM.
26. J. L. Van Etten, unpublished results.
27. R. A. Lamb and L. H. Pinto, Virology 229, 1 (1997).
28. J. Kyte and R. F. Doolittle, J. Mol. Biol. 157, 105 (1982).
29. Channel nomenclature according to (7) and A. Wei, T. Jegla, L. Salkoff, Neuropharmacology 35, 805 (1996).
30. J. D. Thompson, D. G. Higgins, T. J. Gibson, Nucleic Acids Res. 22, 4673 (1994); N. Saitou and M. Nei, Mol. Biol. Evol. 4, 406 (1987). Only the pore region and flanking transmembrane segments were used for the phylogenetic comparisons. For Kir and Kv channels, consensus sequences of the appropriate sub- family were used in the calculations.
31. J. D. Thompson, D. G. Higgins, T. J. Gibson, Nucleic Acids Res. 22, 4673 (1994); N. Saitou and M. Nei, Mol. Biol. Evol. 4, 406 (1987). Only the pore region and flanking transmembrane segments were used for the phylogenetic comparisons. For Kir and Kv channels, consensus sequences of the appropriate sub-family were used in the calculations.
32. We thank H. Terlau, D. Gradmann, and M. Blatt for helpful discussions. Supported in part by MURST in the framework of Cofin-99, NIH grant GM32441 to J.V.E., Small Business Innovative Research grant GM41333 to M.N., Telethon grants (#971 and #296.bi) to D.D. and A.M., and a traveling grant by the SmithKline Beecham foundation to B.P.