Analysis of deletion phenotypes and GFP fusions of 21 novel Saccharomyces cerevisiae open reading frames

Yeast

Volumn 16, Issue 3, 2000, Pages 241-253

  Brachat, Arndt ^a   Liebundguth, Nicole ^a   Rebischung, Corinne ^a   Lemire, Sophie ^a,c   Schärer, Florian ^a   Hoepfner, Dominic ^a   Demchyshyn, Vasyl ^a,b   Howald, Isabelle ^a   Düsterhöft, Andreas ^e   Möstl, Dörte ^e   Pöhlmann, Rainer ^a   Kötter, Peter ^f   Hall, Michael N ^a   Wach, Achim ^a,d   Philippsen, Peter ^a  

^a UNIVERSITY OF BASEL   (Switzerland)

^b INSTITUTE OF HYDROBIOLOGY   (Ukraine)

^c UNIVERSITÉ DE STRASBOURG   (France)

^d Bureco AG   (Switzerland)

^e QIAGEN GMBH   (Germany)

^f Biozentrun N250 R 106   (Germany)

Author keywords

Functional analysis; GFP; PCR targeting; YNL018c; YNL019c; YNL020c; YNL021w; YNL022c; YNL023c; YNL026w; YNL032w; YNL033w; YNL034w; YNL035c; YNL040w; YNL072w; YNL075w; YNL078w; YNL080c; YNL081c; YNL219c; YNL223w; YNL224c; YNL225c

Indexed keywords

CELL NUCLEUS DNA; GREEN FLUORESCENT PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DELETION MUTANT; FLUORESCENCE MICROSCOPY; GENE OVEREXPRESSION; LETHALITY; NONHUMAN; OPEN READING FRAME; PHENOTYPE; PRIORITY JOURNAL; PROMOTER REGION; SACCHAROMYCES CEREVISIAE; SPOROGENESIS; YEAST;

GENE DELETION; GENES, FUNGAL; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; OPEN READING FRAMES; PHENOTYPE; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SPORES, FUNGAL;

EID: 0033962697     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(200002)16:3<241::AID-YEA517>3.0.CO;2-T     Document Type: Article

References (34)

1. Belli G, Gari E, Piedrafita L, Aldea M, Herrero E. 1998. An activator/represser dual system allows tight tetracycline-regulated gene expression in budding yeast. Nucleic Acids Res 26: 942-947.
2. Bucci M, Wente SR. 1997. In vivo dynamics of nuclear pore complexes in yeast. J Cell Biol 136: 1185-1199.
3. Belgareh N, Doye V. 1997. Dynamics of nuclear pore distribution in nucleoporin mutant yeast cells. J Cell Biol 136: 747-759.
4. Brachat A, Kilmartin JV, Wach A, Philippsen P. 1998. Saccharomyces cerevisiae cells with defective spindle pole body outer plaques accomplish nuclear migration via half-bridge-organized microtubules. Mol Biol Cell 9: 977-991.
5. Bullock WO, Fernandez JM, Short JM. 1987. XL1-Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. BioTechniques 5: 376-378.
6. Burda P, te Heesen S, Brachat A, Wach A, Düsterhöft A, Aebi M. 1996. Stepwise assembly of the lipid-linked oligosaccharide in the endoplasmic reticulum of Saccharomyces cerevisiae: identification of the ALC9 gene encoding a putative mannosyl transferase. Proc Natl Acad Sci U S A 93: 7160-7165.
7. Burns N, Grimwade B, Ross-Macdonald PB, Choi EY, Finberg K, Roeder GS, Snyder M. 1994. Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevisiae. Genes Der 8: 1087-1105.
8. Carmen AA, Rundlett SE, Grunstein M. 1996. HDA1 and HDA3 are components of a yeast histone deacetylase (HDA) complex. J Biol Chem 271: 15837-15844.
9. Casari G, Daruvar A, Sander C, Schneider R. 1996. Bioinformatics and the discovery of gene function. Trends Genet 12: 244-245.
10. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. 1994. Green fluorescent protein as a marker for gene expression. Science 263: 802-805.
11. Cho JH, Ha SJ, Kao LR, Megraw TL, Chae CB. 1998. A novel DNA-binding protein bound to the mitochondrial inner membrane restores the null mutation of mitochondrial histone abf2p in Saccharomyces cerevisiae. Mol Cell Biol 18: 5712-5723.
12. Cooper A, Bussey H. 1992. Yeast Kexlp is a Golgiassociated membrane protein: deletions in a cytoplasmic targeting domain result in mislocalization to the vacuolar membrane. J Cell Biol 119: 1459-1468.
13. Frank P, Braunshofer-Reiter C, Wintersberger U. 1998. Yeast RNase H(35) is the counterpart of the mammalian RNase HI, and is evolutionarily related to prokaryotic RNase HII. FEBS Lett 421: 23-26.
14. Goffeau A, Barrell BG, Bussey H. et al. 1996. Life with 6000 genes. Science 274: 546-567.
15. Güldener U, Heck S, Fiedler T, Beinhauer J, Hegemann JH. 1996. A new efficient gene disruption cassette for repeated use in budding yeast. Nucleic Acids Res 24: 2519-2524.
16. Guthrie C, Fink GR. 1991. Guide to yeast genetics and molecular biology. Methods Enzymol 194: 14-15.
17. Heim R, Prasher DC, Tsien RY. 1994. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc Nail Acad Sci U S A 91: 12501-12504.
18. Huxley C, Green ED, Dunham I. 1990. Rapid assessment of 5. cerevisiae mating type by PCR. Trends Genet 6: 236.
19. Lang T, Schaeffeler E, Bernreuther D, Bredschneider M, Wolf DH, Thumm M. 1998. Aut2p and Aut7p, two novel microtubule-associated proteins are essential for delivery of autophagic vesicles to the vacuole. EMBO J 17: 3597-3607.
20. Lim CR, Kimata Y, Oka M, Nomaguchi K, Kohno K. 1995. Thermosensitivity of green fluorescent protein fluorescence utilized to reveal novel nuclear-like compartments in a mutant nucleoporin NSP1. J Biochem 118: 13-17.
21. Nagahashi S, Nakayama H, Hamada K, Yang H, Arisawa M, Kitada K. 1997. Regulation by tetracycline of gene expression in Saccharomyces cerevisiae. Mol Gen Genet 255: 372-375.
22. Niedenthal RK, Riles L, Johnston M, Hegemann JH. 1996. Green fluorescent protein as a marker for gene expression and subcellular localization in budding yeast. Yeast 12: 773-786.
23. Niedenthal RK, Riles L. Güldener U, Klein S, Johnston M, Hegemann JH. 1999. Systematic analysis of S. cerevisiae chromosome VIII genes. Yeast (in press).
24. Philippsen P. et al 1997. The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications. Nature 387 (suppl), 93-98.
25. Planta RJ, Brown AJ, Cadahia JL, Cerdan ME, DeJonge M, Gent ME, Hayes A, Kolen CP, Lombardia LJ, Sefton M, Oliver SG, Thevelein J, Tournu H, van Delft YJ, Verbart DJ, Winderickx J. 1999. Transcript analysis of 250 novel yeast genes from chromosome XIV. Yeast 15: 329-350.
26. Rogers PJ, Yue SB, Stewart PR. 1974. Effects of oxygen tension and glucose repression of mitochondrial protein synthesis in continuous cultures of Saccharomyces cerevisiae. J Bacteriol 118: 523-533.
27. Rothstein R. 1991. Targeting, disruption, replacement, and allel rescue: integrative DNA transformation in yeast. Methods Enzymol 194: 281-301.
28. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular Cloning: A Laboratory Manual (2nd edn). Cold Spring Harbor Laboratory Press: New York.
29. Schiestl RH, Gietz RD. 1989. High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr Genet 16: 339-346.
30. Sikorski RS, Hicter P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
31. Wach A, Brachat A. Pöhlmann R, Philippsen P. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793-1808.
32. Wach A, Brachat A, Philippsen P. 1996. Guidelines for EUROFAN BO program: ORF deletants, plasmid tools, basic functional analyses. EUROFAN web-page at www.mips.biochem.mpg.de/proj/eurofan/ index.html.
33. Wach A, Brachat A, Alberti-Segui C, Rebischung C, Philippsen P. 1997. Heterologous HIS3 marker and GFP reporter modules for Saccharomyces cerevisiae transformation with SFH PCR products. Yeast 13: 1065-1075.
34. Wach A, Brachat A, Rebischung C, Steiner S, Pokorni K, te Heesen S, Philippsen P. 1998. PCR-based gene targeting in 5. cerevisiae. In Methods in Microbiology, vol. 26, Brown AJ, Tuite MF (eds). Academic Press: San Diego, CA; 67-81.