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Stephanopoulos G.N., Aristidou A.A., Nielsen J. Metabolic Engineering: Principles and Methodologies. 1998;Academic Press, San Diego. This is a good general review of the appropriate strategies and critical parameters in metabolic engineering of organisms.
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Stephanopoulos, G.N.1
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Genetic tuning of coryneform bacteria for the overproduction of amino acids
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This is an excellent review of the recent advances in methodology which are influencing the development of recombinant strains of Coryneform bacteria. These are the most industrially relevant amino acid producing organisms. The article illustrates how rational approaches can now complement the classical methods used to engineer these organisms.
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Nampoothiri M., Pandey A. Genetic tuning of coryneform bacteria for the overproduction of amino acids. Process Biochem. 33:1998;147-161. This is an excellent review of the recent advances in methodology which are influencing the development of recombinant strains of Coryneform bacteria. These are the most industrially relevant amino acid producing organisms. The article illustrates how rational approaches can now complement the classical methods used to engineer these organisms.
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The article describes the use of both rational molecular biology and mutagenesis/selection methods to produce an organism capable of stable L-cysteine overproduction. This is an excellent example of the appropriate use of both approaches to identify an enzyme variant free from natural regulation and a host organism tolerant to the accumulation of a primary metabolite which would normally be catabolised.
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Nakamori S., Kobayashi S-I., Kobayashi C., Takagi H. Overproduction of L-cysteine and L-cystine by Escherichia coli strains with a genetically altered serine acetyltransferase. Appl Environ Microbiol. 64:1998;1607-1611. The article describes the use of both rational molecular biology and mutagenesis/selection methods to produce an organism capable of stable L-cysteine overproduction. This is an excellent example of the appropriate use of both approaches to identify an enzyme variant free from natural regulation and a host organism tolerant to the accumulation of a primary metabolite which would normally be catabolised.
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Taylor P.P., Pantaleone D.P., Senkpeil R.F., Fotheringham I.G. Novel biosynthetic approaches to the production of unnatural amino acids using transaminases. Trends Biotechnol. 16:1999;412-418. The article describes a number of examples where transaminases have been used to produce non-proteinogenic and unnatural amino acids. In many cases, the incorporation of additional enzymes has significantly impacted the usefulness of reactions which would otherwise display significant limitations.
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Taylor P.P., Grinter N.J., McCarthy S.L., Pantaleone D.P., Ton J.L., Yoshida R.K., Fotheringham I.G. D-phenylalanine biosynthesis using Escherichia coli: creation of a new metabolic pathway. Book of Abstracts, 217th ACS National Meeting Anaheim Calif March. 1999;21-25.
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