Construction and characterization of an oxalic acid nonproducing strain of Aspergillus niger

Metabolic Engineering

Volumn 2, Issue 1, 2000, Pages 34-41

  Pedersen, Henrik ^a   Christensen, Bjarke ^a   Hjort, Carsten ^b   Nielsen, Jens ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b NOVO NORDISK A S   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMICAL PATHWAY; CELL METABOLISM; CITRIC ACID; DOWNSTREAM PROCESSING; FUNGAL GROWTH; GENE DISRUPTION; GENETIC STRAIN; GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOSE METABOLISM; GROWTH RATE; INDUSTRIAL ENZYME; METABOLIC ENGINEERING; OAH GENE; OXALIC ACID; OXALOACETATE HYDROLASE; PENTOSE PHOSPHATE; WILD RELATIVE;

ASPERGILLUS NIGER;

EID: 0033916304     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1006/mben.1999.0136     Document Type: Article

References (19)

1. Balmforth A. J., Thomson A. Isolation and characterization of glyoxylate dehydrogenase from the fungus Sclerotium rolfsii. Biochem. J. 218:1984;113-118.
2. Bercovitz A., Peleg Y., Battat E., Rokem J. S., Goldberg I. Localization of pyruvate carboxylase in organic acid-producing Aspergillus strains. Appl. Environ. Microbiol. 56:1990;1594-1597.
3. Christensen B., Nielsen J. Isotopomer analysis using GC-MS. Metab. Eng. 1:1999;282-290.
4. Henriksen C. M., Christensen L. H., Nielsen J., Villadsen J. Growth energetics and metabolic fluxes in continuous cultures of Penicillium chrysogenum. J. Biotechnol. 45:1996;149-164.
5. Holm K. A. Automatic spectrophotometric determination of amyloglucosidase activity using p-nitrophenyl-a-D-glucopyranoside and a flow injection analyser. Analyst. 111:1986;927-929.
6. Houck D. R., Inamine E. Oxalic acid biosynthesis and oxalate acetylhydrolase activity in Streptomyces cattleya. Arch. Biochem. Biophys. 259:1987;58-65.
7. Jones E. W., Fink G. R. Regulation of amino acid and nucleotide biosynthesis in yeast. Strathern J. N., Jones E. W., Broach J. R. The Molecular Biology of the Yeast Saccharomyces. Metabolism and Gene Expression. 1982;181-299 Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
8. Kubicek C. P., Schreferl-Kunar G., Wöhrer W., Röhr M. Evidence for a cytoplasmic pathway of oxalate biosynthesis in Aspergillus niger. Appl. Environ. Microbiol. 55:1988;633-637.
9. Marx A., de Graaf A. A., Wiechert W., Eggeling L., Sahm H. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 49:1996;111-129.
10. Nissen T. L., Schulze U., Nielsen J., Villadsen J. Flux distributions in anaerobic, glucose-limited continuous cultures of Saccharomyces cerevisiae. Microbiology. 143:1997;203-218.
11. Pedersen H., Beyer M., Nielsen J. Glucoamylase production in batch, chemostat and fed-batch cultivations by Aspergillus niger. Appl. Microbiol. Biotechnol. 1999a.
12. Pedersen H., Carlsen M., Nielsen J. Identification of enzymes and quantification of metabolic fluxes in the wild type and in a recombinant Aspergillus oryzae strain. Appl. Environ. Microbiol. 65:1999b;11-19.
13. Pedersen H., Hjort C., Nielsen J. Cloning and characterization of oah, the gene encoding oxaloacetate hydrolase from Aspergillus niger. Mol. Gen. Genetics. 1999c.
14. Roehr M., Kubicek C. P., Kominek J. Industrial acids and other small molecules. Bennett J. W., Klich M. A. Aspergillus, Biology and Industrial Applications. 1992;91-153 Butterworth-Heinemann, Stoneham.
15. 13C tracer experiments and metabolite balancing for metabolic flux analysis: Comparing two approaches. Biotechnol. Bioeng. 58:1998;254-257.
16. Vallino J. J., Stephanopoulos G. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol. Bioeng. 41:1993;633-646.
17. Wittmann C., Heinzle E. Mass spectrometry for metabolic flux analysis. Biotechnol. Bioeng. 62:1999;739-750.
18. Woodcock D. M., Crowther P. J., Doherty J., Jefferson S., DeCruz E., Noyer-Weidner M., Smith S. S., Michael M. Z., Graham M. W. Quantitative evaluation of Escherichia coli host strains for tolerance to cytosine methylation in plasmid and phage recombinants. Nucleic Acids Res. 17:1989;3469-3478.
19. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 33:1985;103-119.