An antagonist decoy receptor and a death domain-containing receptor for TRAIL

Science

Volumn 277, Issue 5327, 1997, Pages 815-818

  Pan, Guohua ^a   Ni, Jian ^b   Wei, Ying Fei ^b   Yu, Guo Iiang ^b   Gentz, Reiner ^b   Dixit, Vishva M ^a,c  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b GLAXOSMITHKLINE   (United States)

^c GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL RECEPTOR; CYTOTOXIC FACTOR; GENE PRODUCT;

ANIMAL CELL; APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CELL DEATH; ETIOLOGY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION;

AMINO ACID SEQUENCE; ANIMALS; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CASPASE 10; CASPASE 8; CASPASE 9; CASPASES; CELL LINE, TRANSFORMED; CYSTEINE ENDOPEPTIDASES; HELA CELLS; HUMANS; LIGANDS; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; PROTEIN SORTING SIGNALS; RECEPTORS, TNF-RELATED APOPTOSIS-INDUCING LIGAND; RECEPTORS, TUMOR NECROSIS FACTOR; SEQUENCE ALIGNMENT; SIGNAL TRANSDUCTION; TNF-RELATED APOPTOSIS-INDUCING LIGAND; TRANSFECTION; TUMOR CELLS, CULTURED; TUMOR NECROSIS FACTOR DECOY RECEPTORS; TUMOR NECROSIS FACTOR-ALPHA;

ANIMALIA; MAMMALIA;

EID: 0030762815     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5327.815     Document Type: Article

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28. Northern (RNA) blot analysis was performed with multiple tissue blots (Clontech, Palo Alto, CA) according to the manufacturer's instructions.
29. DR5, DR5Δ (amino acids 52 to 411), and TRID were cloned into pCMV1FLAG vector (IBI, Kodak). cDNAs encoding the extracellular domains of DR5 (amino acids 52 to 180) and TRID (amino acids 24 to 163) were obtained by polymerase chain reaction and cloned into a modified pCMV1FLAG vector that allowed for in-frame fusion with the Fc portion of human IgG. The constructs expressing DR4, soluble TRAIL, and TNF-α have been described (4).
30. Cell death assays were done as described (5). MCF7 and HeLa cells were transfected with the lipofectamine procedure (BRL, Grand Island, NY), according to the manufacturer's instructions. 293 cells and 293T cells were transfected with calcium phosphate precipitation.
31. In vivo interaction assays have been described elsewhere (3).
32. The preparation of receptor-Fc fusions and ligands and in vitro binding assays has been described (4).
33. We thank A. M. Chinnaiyan, D. Chaudhary, H. Duan, S. Hu, E. Humke, K. Luetke, J. McCarthy, M. Muzio, H.-N. Nguyen, K. O'Rourke, Q. Song, and C. Vincenz for useful discussions and reagents; Y. Kuang for technical help; I. Jones for preparing figures; and J. DeJohn for secretarial assistance. Supported by NIH grants ES08111 and DAMD17-96-1-6085.