Control of TRAIL-induced apoptosis by a family of signaling and decoy receptors

Science

Volumn 277, Issue 5327, 1997, Pages 818-821

  Sheridan, James P ^a   Marsters, Scot A ^a   Pitti, Robert M ^a   Gurney, Austin ^a   Skubatch, Maya ^a   Baldwin, Daryl ^a   Ramakrishnan, Lakshmi ^a   Gray, Christa L ^a   Baker, Kevin ^a   Wood, William I ^a   Goddard, Audrey D ^a   Godowski, Paul ^a   Ashkenazi, Avi ^a  

^a GENENTECH INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL RECEPTOR; CELL SURFACE PROTEIN; GLYCOPHOSPHOLIPID;

ANIMAL CELL; APOPTOSIS; ARTICLE; CANCER CELL; CELL DEATH; ETIOLOGY; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; APOPTOSIS; APOPTOSIS REGULATORY PROTEINS; CELL MEMBRANE; CELLS, CULTURED; GLYCOSYLPHOSPHATIDYLINOSITOLS; HELA CELLS; HUMANS; LIGANDS; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; NF-KAPPA B; RECEPTORS, TNF-RELATED APOPTOSIS-INDUCING LIGAND; RECEPTORS, TUMOR NECROSIS FACTOR; SIGNAL TRANSDUCTION; TNF-RELATED APOPTOSIS-INDUCING LIGAND; TRANSFECTION; TUMOR CELLS, CULTURED; TUMOR NECROSIS FACTOR DECOY RECEPTORS; TUMOR NECROSIS FACTOR-ALPHA;

ANIMALIA;

EID: 0030792712     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5327.818     Document Type: Article

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36. Transient transfection of 293 cells by pRK5 itself resulted in sensitization to TRAIL-induced apoptosis relative to untransfected cells. The levels of background apoptosis in 293 cells as measured by morphology, directly in the culture dish (Fig. 4), were lower than the levels measured by fluorescence-activated cell sorting (FACS) analysis after harvest and staining of the cells with annexin V (9).
37. 125I-TRAIL binding (25), consistent with the 39% sensitization to TRAIL-induced apoptosis of cells treated similarly with PI-PLC (Fig. 4B).
38. We thank C. Clark for comments; D. Goeddel for CrmA and FADD-DN plasmids; V. Dixit for DR4 and DFW-IgG plasmids; A. Rosenthal for PI-PLC; P. Juhrani, P. Ng, and M. Vasser for DNA synthesis; J. Lee and A. Gray for cDNA libraries; C. Watanabe for sequence analysis; M. Hamner for DNA sequencing; J. Tropea and W. Henzel for protein sequencing; S. Leung, J. Swartz, C. Blackie, and R. Pai for generating TRAIL; and the Genentech signal sequence trap team for help in cloning and expression.