Expression of the the cefG gene is limiting for cephalosporin biosynthesis in Acremonium chrysogenum

Applied Microbiology and Biotechnology

Volumn 48, Issue 5, 1997, Pages 606-614

  Gutierrez S ^a,b   Velasco, J ^a,b   Marcos, A T ^a,b   Fernandez F J ^a,b   Fierro, F ^a,b   Barredo, J L ^c   Diez B ^c   Martin J F ^a,b  

^a UNIVERSITY OF LEÓN   (Spain)

^b Institute of Biotechnology INBIOTEC Parque Científico de León   (Spain)

^c Avenida de Antibioóticos 59 61   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

CEPHALOSPORIN; DEACETYLCEPHALOSPORIN C; GLUTAMATE DEHYDROGENASE;

ACETYLATION; ANTIBIOTIC BIOSYNTHESIS; ARTICLE; ASPERGILLUS NIDULANS; ASPERGILLUS NIGER; CEPHALOSPORIUM ACREMONIUM; CONTROLLED STUDY; ENZYME ACTIVITY; GENE EXPRESSION; GENE EXPRESSION REGULATION; NONHUMAN; PENICILLIUM CHRYSOGENUM; PROMOTER REGION;

ACETYLTRANSFERASES; ACREMONIUM; CEPHALOSPORINS; CHROMOSOME MAPPING; CLONING, MOLECULAR; DNA, FUNGAL; GENE AMPLIFICATION; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; GLUCAN 1,4-ALPHA-GLUCOSIDASE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; HYDRO-LYASES; IMMUNOBLOTTING; OXIDOREDUCTASES; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINATION, GENETIC; TRANSCRIPTION, GENETIC;

ACREMONIUM CHRYSOGENUM; ASPERGILLUS NIGER; CEPHALOSPORIUM; EMERICELLA NIDULANS; PENICILLIUM; PENICILLIUM CHRYSOGENUM; TRIXIS;

EID: 0030779773     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051103     Document Type: Article

References (34)

1. Andrianapoulos A, Timerlake WE (1994) The Aspergillus nidulans abaA gene encodes a transcriptional activator that acts as a genetic switch to control development. Mol Cell Biol 14: 2503-2525
2. Ausubel FM, Brent R, Kingston RE, Moore D, Smith JA, Seidman JG, Struhl K (eds) (1987) Current protocols in molecular biology, Wiley, New York
3. Ballance DJ (1986) Sequences important for gene expression in filamentous fungi. Yeast 2: 229-236
4. Demain AL (1983) Strain exchange between industry and academia. ASM News 49: 431
5. Dhawale SA, Lane AC (1993) Compilation of sequence-specific DNA-binding proteins implicated in transcriptional control in fungi. Yeast 21: 5537-5546
6. Dunbar BS, Schwoebel ED (1990) Preparation of polyclonal antibodies. Methods Enzymol 182: 663-670
7. Feng B, Friedlin E, Marzluf GA (1995) Nuclear DNA-binding proteins which recognize the intergenic control region of penicillin biosynthetic genes. Curr Genet 27: 351-358
8. Fidel S, Doonana JH, Morris NR (1988) Aspergillus nidulans contains a single actin gene which has unique intron locations and encodes a gamma-actin. Gene 70: 282-293
9. Fujisawa Y, Kanzaki T (1975) Role of acetyl-CoA:deacetylcephalosporin C acetyltransferase in cephalosporin biosynthesis by Cephalosporium acremonium. Agric Biol Chem 39: 2043-2048
10. Fujisawa Y, Shirafuji H, Kida M, Nara K, Yoneda M, Kanzaka T (1973) New findings on CPC biosynthesis. Nature New Biol 246: 154
11. Fujisawa Y, Shirafuji H, Kida M, Nara K, Yoneda M, Kanazaki T (1975) Accumulation of deacetylcephalosporin C by cephalosporin C negative mutants of Acremonium chrysogenum. Agric Biol Chem 39: 1295-1302
12. Gutiérrez S, Díez B, Alvarez E, Barredo JL, Martín JF (1991) Expression of the penDE gene of Penicillium chrysogenum encoding isopenicillin N acyltransferase in Cephalosporium acremonium: production of benzylpenicillin by the transformants. Mol Gen Genet 225: 56-64
13. Gutiérrez S, Velasco J, Fernández FJ, Martín JF (1992) The cefG gene of Cephalosporium acremonium is linked to the cefEF gene and encodes a deacetylcephalosporin C acetyltransferase closely related to homoserine O-acetyltransferase. J Bacteriol 174: 3056-3064
14. Harlow E, Lane D (1988) Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
15. Hinnebusch AG (1993) Gene-specific translational control of the yeast GCN4 gene of phosphorylation of eurkaryotic initiation factor 2. Mol Microbiol 10(2): 215-223
16. Hinnen A, Nüesch J (1976) Enzymatic hydrolysis of cephalosporin C by extracellular acetylhydrolase of Cephalosporium acremonium. Antimicrob Agents Chemother 9: 824-830
17. Mackenzie DA, Jeenes DJ, Belshaw NJ, Archer DB (1993) Regulation of secreted protein production by filamentous fungi: recent developments and perspectives. J Gen Microbiol 139: 2295-2307
18. Martín JF, Gutiérrez S, Fernández FJ, Velasco J, Fierro F, Marcos AT, Kosalková K (1994) Expression of genes and processing of enzymes for the biosynthesis of penicillins and cephalosporins. Antonie van Leeuwenhoek 65: 227-243
19. Martín JF, Gutiérrez S, Demain AL (1997) β-Lactams. In: Anke, T (ed) Fungal biotechnology. Chapman & Hall, Weinheim (in press)
20. Mathison L, Soliday C, Stepan T, Aldrich T, Rambosek J (1993) Cloning, characterization, and use in strain improvement of the Cephalosporium acremonium gene cefG encoding acetyl transferase. Curr Genet 23: 33-41
21. Matsuda A, Sugiura H, Matsuyama K, Matsumoto H, Ichikawa S, Komatsu K-I (1992) Molecular cloning of acetyl coenzyme A:deacetylcephalosporin CO-acetyltransferase cDNA from Acremonium chrysogenum: sequence and expression of catalytic activity in yeast. Biochem Biophys Res Commun 182: 995-1001
22. Nüesch J, Heim J, Treichler H-J (1987) The biosynthesis of sulfur-containing β-lactam antibiotics. Annu Rev Microbiol 41: 51-57
23. Punt PJ, Oliver RP, Dingemanse MA, Pouwels PH, Hondel CAMJJ van den (1987) Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56: 117-124
24. Punt PJ, Dingemanse MA, Kuyvenhoven J, Soede RDM, Pouwels PH, Hondel CAMJJ van den (1990) Functional elements in the promoter region of the Aspergillus nidulans gpdA gene, encoding glyceraldehyde-3-phosphate dehydrogenase. Gene 93: 101-109
25. Punt PJ, Kramer C, Kuyvenhoven A, Pouwels PH, Hondel CAMJJ van den (1992) An upstream activating sequence from the Aspergillus nidulans gpdA gene. Gene 120: 67-73
26. Ramos FR, López-Nieto MJ, Martín JF (1986) Coordinate increase of isopenicillin N synthetase, isopenicillin N epimerase and deacetoxycephalosporin C synthetase in a high cephalosporin-producing mutant of A. chrysogenum and simultaneous loss of the three enzymes in a non-producing mutant. FEMS Microbiol Lett 35: 123-127
27. Scheidegger A, Gutzwiller A, Kuenzi MT, Fiechter A, Nüesch J (1985) Investigation of acetyl-CoA:deacetylcephalosporin C O-acetyltransferase of Cephalosporium acremonium. J Biotechnol 3: 109-117
28. Shen Y-Q, Wolfe S, Demain AL (1986) Levels of isopenicillin N synthetase and deacetoxycephalosporin C synthetase in Cephalosporium acremonium producing high and low levels of cephalosporin C. Biotechnology 4: 61-64
29. Skatrud PL, Tietz AJ, Ingolia TD, Cantwell CA, Fisher DL, Chapman JL, Queener SW (1989) Use of recombinant DNA to improve production of cephalosporin C by Cephalosporium acremonium. Biotechnology 7: 476-485
30. Tabor S, Richardson CC (1985) A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc Natl Acad Sci USA 82: 1074-1078
31. Tilburn J, Sarkar S, Widdick DA, Espeso EA, Orejas M, Mungroo J, Peñalva MA, Arst HN (1995) The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH. EMBO J 14: 779-790
32. Timberlake WE (1991) Cloning and analysis of fungal genes. In: Bennett JW, Lasure LL (eds) More gene manipulations in fungi. Academic Press, San Diego, Calif, pp 51-85
33. Velasco J, Gutiérrez S, Fernández FJ, Marcos AT, Arenós C, Martín JF (1994) Exogenous methionine increases levels of mRNAs transcribed from pcbAB, pcbC, and cefEF gene, encoding enzymes of the cephalosporin biosynthetic pathway, in Acremonium chrysogenum. J Bucteriol 176: 985-991
34. Zanca DM, Martín JF (1983) Carbon catabolite regulation of the conversion of penicillin N into cephalosporin C. J Antibiot (Tokyo) 36: 700-708