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note
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All studies involving mice were carried out under protocols approved by the Institutional Animal Care and Use Committee of Fordham University.
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1842381357
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note
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Antibodies against GP96 (9G10.F8.2) and HSP70 (BRM22) were obtained from NeoMarkers (Fremont, CA).
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+ T cells by intraperitoneal (ip) injection of 250 μl of 1:8 diluted GK1.5 ascites or YTS-169.4 ascites, respectively. Injections were continued biweekly. NK cells were depleted by ip injection of 200 μg of purified monoclonal antibody NK1.1 every week. Mice in the control group were injected with 200 μg of rat or mouse Ig once per week. The efficiency of depletion was confirmed by flow cytometry, and the specific depletion was 90 to 100%.
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We thank S. Porcino for preparing the figures. Supported by NIH grants CA44786 and CA64394 and through an agreement with Antigenics (New York).
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