Chromatographic determination of 8-oxo-7,8-dihydro-2'-deoxyguanosine in cellular DNA: A validation study

Free Radical Research

Volumn 33, Issue 3, 2000, Pages 243-260

  Duez, P ^a   Helson, M ^a   Some, T I ^a   Dubois, J ^a   Hanocq, M ^a  

^a UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

Author keywords

8 OH dG; 8 oxo dG; Analytical validation; Biomarker; DNA damage; Oxidative stress

Indexed keywords

8 HYDROXYDEOXYGUANOSINE; CELL DNA; CELL MARKER; NUCLEASE;

ARTICLE; CHEMOSENSITIVITY; CHROMATOGRAPHY; DIAGNOSTIC ACCURACY; DNA DAMAGE; ENZYME ACTIVITY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; VALIDATION PROCESS;

EID: 0033832182     PISSN: 10715762     EISSN: None     Source Type: Journal    
DOI: 10.1080/10715760000301411     Document Type: Article

References (64)

1. Analytical technologies for lipid oxidation products analysis
2. Biomarkers of free radical damage. Applications in experimental animals and in humans
3. The comet assay: A comprehensive review
4. Thymine glycol and thymidine glycol in human and rat urine: A possible assay for oxidative DNA damage
5. Can oxidative DNA damage be used as a biomarker of cancer risk in humans? Problems, resolutions and preliminary results from nutritional supplementation studies
6. Mass spectrometric analysis of 2-deoxyribonucleoside and 2'-deoxyribonucleotide adducts with aldehydes derived from lipid peroxidation
7. Determination of steady-state levels of oxidative DNA base modifications in mammalian cells by means of repair endonucleases
8. Detection of single-strand breaks and formamidopyrimidine-DNA glycosylase-sensitive sites in DNA of cultured human fibroblasts
9. Gel electrophoresis detection of 7,8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine via oxidation by Ir(IV)
10. Detection of purine lesions in cellular DNA using single cell gel electrophoresis with Fpg protein
11. Hydroxyl free radical adduct of deoxyguanosine: Sensitive detection and mechanisms of formation
12. Chemical determination of oxidative DNA damage by gas chromatography-mass spectrometry
13. Micellar electrokinetic capillary chromatography of 8-hydroxydeoxyguanosine and other oxidized derivatives of DNA
14. Detection and quantitation of 8-hydroxydeoxyguanosine 5'-monophosphate in X-irradiated calf-thymus DNA by fluorescence postlabeling
15. Chemical and biochemical postlabeling methods for singling out oxidative DNA lesions
16. Immunoaffinity isolation of urinary 8-hydroxy-2'-deoxyguanosine and 8-hydroxyguanine and quantitation of 8-hydroxy-2'-deoxyguanosine in DNA by polyclonal antibodies
17. Quantitative immunoanalysis of promutagenic 8-hydroxy-2'-deoxyguanosine in oxidized DNA
18. Chemical determination of free radical-induced damage to DNA
19. Cancer risk and oxidative DNA damage in man
20. Detection of DNA adducts by high-performance liquid chromatography with electrochemical detection
21. Singlet oxygen induced DNA damage
22. Evidence for the accumulation of oxidative stress during cellular ageing of human diploid fibroblasts
23. Analysis of a form of oxidative DNA damage, 8-hydroxy-2'-deoxyguanosine, as a marker of cellular oxidative stress during carcinogenesis
24. 8-Hydroxyguanine, an abundant form of oxidative DNA damage, causes G → T and A → C substitutions
25. Miscoding during DNA synthesis on damaged DNA templates catalysed by mammalian cell extracts
26. Repair and mutagenic potency of 8-oxoG:A and 8-oxoG:C base pairs in mammalian cells
27. Problems in the measurement of 8-oxoguanine in human DNA. Report of a workshop, DNA oxidation, held in Aberdeen, UK, 19-21 January, 1997
28. Urinary 8-hydroxy-2'-deoxyguanosine as a biological marker of in vivo oxidative DNA damage
29. Effect of smoking cessation on oxidative DNA modification estimated by 8-oxo-7,8-dihydro-2'-deoxyguanosine excretion
30. Determination of 8-hydroxydeoxyguanosine in human cells under oxygen-free conditions
31. Induction of reactive oxygen species without 8-hydroxydeoxyguanosine formation in DNA of initiated mouse keratinocytes treated with 12-O-tetradecanoylphorbol-13-acetate
32. Measurement of products of free radical attack on nucleic acids
33. Improvements in the analytical method for 8-hydroxydeoxyguanosine in nuclear DNA
34. Efficiency of an automatic peak purity control procedure in high performance liquid chromatography-photodiode array coupling based on evolving factor analysis
35. Formation of 8-hydroxydeoxyguanosine, hydroxyl free radical adduct of DNA in granulocytes exposed to the tumor promoter, tetradecanoylphorbolacetate
36. Measurement of 8-OHdG in DNA by HPLC/ECD: The importance of DNA purity
37. The role of prostaglandin H synthase-mediated metabolism in the induction of oxidative DNA damage by BHA metabolites
38. Proposed mechanism for the photodynamic generation of 8-oxo-7,8-dihydro-2'-deoxyguanosine produced in cultured cells by exposure to lomefloxacin
39. A novel HPLC procedure for the analysis of 8-oxoguanine in DNA
40. Improved genomic/nuclear DNA extraction for 8-hydroxydeoxyguanosine analysis of small amounts of rat liver tissue
41. Induction of oxidative DNA base damage in human skin cells by UV and near visible radiation
42. Micellar electrokinetic capillary chromatography of 8-oxoguanine and other bases of DNA
43. Immunoperoxidase detection of 8-hydroxydeoxyguanosine in aflatoxin B1-treated rat liver and human oral mucosal cells
44. Formation of 8-hydroxydeoxyguanosine in rat kidney DNA after administration of N-ethyl-N-hydroxyethylnitrosamine
45. Formation and persistence of 8-oxoguanine in rat lung cells as an important determinant for tumor formation following particle exposure
46. Oxidative DNA damage in human cells: The influence of antioxidants and DNA repair
47. DNA oxidation matters: The HPLC-electrochemical detection assay of 8-oxo-deoxyguanosine and 8-oxo-guanine
48. Phenol isolation of DNA yields higher levels of 8-oxodeoxyguanosine compared to pronase E isolation
49. Methods used for analyses of environmentally damaged nucleic acids
50. 2
51. Evaluation of 8-hydroxydeoxyguanosine, a typical oxidative DNA damage, in human leukocytes
52. Validation of chromatographic methods in biomedical analysis. Viewpoint and discussion
53. Guide de validation analytique. Rapport d'une commission SFSTP
54. Facts and artifacts in the measurement of oxidative base damage to DNA
55. Facts about the artifacts in the measurement of oxidative DNA base damage by gas chromatography mass spectrometry
56. Detection and quantification of 8-hydroxydeoxyguanosine adducts in peripheral blood of people exposed to ionizing radiation
57. Assays of oxidative DNA damage biomarkers 8-oxo-2'-deoxyguanosine and 8-oxoguanine in nuclear DNA and biological fluids by high-performance liquid chromatography with electrochemical detection
58. Reduction of oxidation during the preparation of DNA and analysis of 8-hydroxy-2'-deoxyguanosine
59. X-radiation induces 8-hydroxy-2'-deoxyguanosine formation in vivo in rat mammary gland DNA
60. ESCODD: European Standards Committee on Oxidative DNA damage