Misinterpretation of DNA sequence data generated by polymerase chain reactions

Molecular Immunology

Volumn 37, Issue 6, 2000, Pages 329-

  Blanden, Robert V ^a   Steele, Edward J ^b  

^a Biology and Environment   (Australia)

^b UNIVERSITY OF WOLLONGONG   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; DNA;

COMPLEMENTARITY DETERMINING REGION; DNA SEQUENCE; EVALUATION; GENE MUTATION; GENE REARRANGEMENT; IMMUNOGLOBULIN VARIABLE REGION; LETTER; MUTATION RATE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

ANIMALS; COMPLEMENTARITY DETERMINING REGIONS; GENE REARRANGEMENT, B-LYMPHOCYTE; HUMANS; MICE; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA;

EID: 0033824993     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0161-5890(00)00051-1     Document Type: Letter

References (6)

1. Use of family specific leader region primers for PCR amplification of the human heavy chain variable region gene repertoire
2. Enhanced evolvability in immunoglobulin V genes under somatic hypermutation
3. Strong intrinsic biases towards mutation and conservation of bases in human IgV(H) genes during somatic hypermutation prevent statistical analysis of antigen selection
4. Inherent properties of somatic hypermutation as revealed by human non-productive V(H)6 immunoglobulin rearrangements
5. Targeting of non-1g sequences in place of the V segment by somatic hypermutation
6. PCR amplification of murine immunoglobulin germline V genes: Strategies for minimization of recombination artefacts