Voltage-dependent structural interactions in the Shaker K+ channel

Journal of General Physiology

Volumn 115, Issue 2, 2000, Pages 123-138

  Tiwari Woodruff, Seema K ^a   Lin, Meng Chin A ^a   Schulteis, Christine T ^a,b   Papazian, Diane M ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

Conformational changes; Cysteine mutagenesis; Transmembrane segment; Voltage clamp

Indexed keywords

CARRIER PROTEIN; POTASSIUM; POTASSIUM CHANNEL;

ARTICLE; CHANNEL GATING; CONFORMATIONAL TRANSITION; MOLECULAR MODEL; NONHUMAN; PHENOTYPE; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS; VOLTAGE CLAMP;

ANIMALS; ELECTROPHYSIOLOGY; MEMBRANE POTENTIALS; MUTAGENESIS, SITE-DIRECTED; MUTATION; OOCYTES; PATCH-CLAMP TECHNIQUES; POTASSIUM CHANNELS; PROTEIN CONFORMATION; XENOPUS;

EID: 0033818976     PISSN: 00221295     EISSN: None     Source Type: Journal    
DOI: 10.1085/jgp.115.2.123     Document Type: Article

References (50)

1. + channel
2. + channel gating
3. Inactivation of the sodium channel. I. Sodium current experiments
4. + channels: II. The components of gating currents and a model of channel activation
5. + channel with fluorescence
6. + channel
7. Activation-dependent subconductance levels in the drk1 K channel suggest a subunit basis for ion permeation and gating
8. A structural vignette common to voltage sensors and conduction pores: Canaliculi
9. Do salt bridges stabilize proteins? A continuum electrostatic analysis
10. On the use of thiol-modifying agents to determine channel topology
11. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
12. Biophysical and molecular mechanisms of Shaker potassium channel inactivation
13. + channels: Effects of alterations in the carboxy-terminal region
14. Determinants of voltage-dependent gating and open-state stability in the S5 segment of Shaker potassium channels
15. A general method for rapid site-directed mutagenesis using the polymerase chain reaction
16. + channel S4
17. Mutations in the S4 region isolate the final voltage-dependent cooperative step in potassium channel activation
18. + channel during gating
19. + channel inner pore revealed by stoichiometrlc covalent modification
20. Direct physical measure of conformational rearrangement underlying potassium channel gating
21. Covalent modification of engineered cysteines in the nicotinic acetylcholine receptor agonist-binding domain inhibits receptor activation
22. + channel by three amino acid substitutions
23. Potassium channel α and β subunits assemble in the endoplasmic reticulum
24. + channels
25. A new approach to the study of transient protein conformations: The formation of a semiburied salt link in the folding pathway of barnase
26. How does an ion channel sense voltage?
27. + channel
28. + channels
29. Glycosylation of Shaker potassium channel protein in insect cell culture and in Xenopus oocytes
30. The 'megaprimer' method of site-directed mutagenesis
31. Activation of Shaker potassium channels. III. An activation gating model for wild-type and V2 mutant channels
32. Subunit folding and assembly steps are interspersed during Shaker potassium channel biogenesis
33. + channel are not linked by a disulfide bond
34. Multiple potassium channel components are produced by alternative splicing at the Shaker locus in Drosophila
35. + channel
36. Gating current noise produced by elementary transitions in Shaker potassium channels
37. Uncharged S4 residues and cooperativity in voltage-dependent potassium channel activation
38. + channel
39. Histidine scanning mutagenesis indicates full translocation of two charges of the Shaker K channel voltage sensor
40. Accessibility studies of Shaker K channel S4 residues by histidine-scanning mutagenesis
41. Electrostatic potential of the acetylcholine binding sites in the nicotinic receptor probed by reactions of binding site cysteines with charged methanethiosulfonates
42. Expression of functional potassium channels from Shaker cDNA in Xenopus oocytes
43. Importance of two buried salt bridges in the stability and folding pathway of barnase
44. + channel subunits
45. Molecular basis of charge movement in voltage-gated sodium channels
46. How does the W434F mutation block current in Shaker potassium channels?
47. Shaker potassium channel gating. III: Evaluation of kinetic models for activation
48. Selectivity changes during activation of mutant Shaker potassium channels
49. Intermediate conductances during deactivation of heteromultimeric Shaker potassium channels