Rapid genotyping by MALDI-monitored nuclease selection from probe libraries

Nature Biotechnology

Volumn 18, Issue 11, 2000, Pages 1213-1216

  Stoerker, Jay ^a,b,c   Mayo, Jason D ^a,b,d   Tetzlaff, Charles N ^a,b,d   Sarracino, David A ^a,b   Schwope, Ina ^a,b   Richert, Clemens ^a,b,d  

^a TUFTS UNIVERSITY   (United States)

^b Department of Pharmacology and Experimental Therapeutics ^*  (United States)

^c BRUKER DALTONIK GMBH   (Germany)

^d UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; NUCLEASE;

ANALYTIC METHOD; ARTICLE; DNA PROBE; GENE LIBRARY; GENOTYPE; HUMAN; LABORATORY TEST; MASS SPECTROMETRY; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SINGLE NUCLEOTIDE POLYMORPHISM;

ALLELES; BASE SEQUENCE; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; EXONS; GENE LIBRARY; GENOTYPE; HETEROZYGOTE; HUMANS; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE PROBES; PHOSPHODIESTERASE I; PHOSPHORIC DIESTER HYDROLASES; POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 0033767565     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/81226     Document Type: Article

References (21)

1. The future of genetic studies of complex human diseases
2. Functional genomics
3. Detection of point mutations by solid phase methods
4. DNA variations in the future of human genetics
5. Methods for detection of point mutations: Performance and quality assessment
6. Discrimination of single-nucleotide polymorphisms in human DNA using peptide nucleic acid probes detected by MALDI-TOF mass spectrometry
7. Single-nucleotide polymorphism analysis by MALDI-TOF mass spectrometry
8. Four common mutations of the cytathione B-synthase gene detected by multiplex PCR and matrix-assisted laser desorption/ionization time-of-flight spectrometry
9. Competitive oligonucleotide single-base extension combined with mass spectrometric detection for mutation screening
10. High level multiplex genotyping by MALDI-TOF mass spectrometry
11. Chip-based genotyping by mass spectrometry
12. MALDI-TOF mass spectrometric typing of single nucleotide polymorphisms with mass tagged ddNTPs
13. Genotyping by mass spectrometric analysis of short DNA fragments
14. Direct genetic analysis by matrix-assisted laser desorption ionization mass spectroscopy
15. Selection of modified oligonucleotides with increased target affinity via MALDI-monitored nuclease survival assays
16. Quantitative MALDI-TOF spectrometry of oligonucleotides and a nuclease assay
17. Genomic DNA sequence of the cystic fibrosis transmembrane conductance regulator (CFTR) gene
18. Sequence dependence for the energetics of terminal mismatches in ribooligonucleotides
19. Multiplex PCR amplification from the CFTR gene using DNA prepared from buccal brushes/swabs