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Volumn 6, Issue 21, 1996, Pages 2543-2548

Quantitative MALDI-TOF ms of oligonucleotides and a nuclease assay

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEASE;

EID: 0030571297     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/0960-894X(96)00465-9     Document Type: Article
Times cited : (64)

References (27)
  • 18
    • 0011921439 scopus 로고    scopus 로고
    • note
    • -7 Torr (drift tube) from 100 laser shots at 2 Hz laser frequency and 20 mV preamplifier voltage in linear, negative mode on a Bruker, BIFLEX® spectrometer at 17.5 kV extraction voltage. Matrix ions were suppressed by 2 kV deflection up to m/z 600. Five spectra were accumulated per target with best signals usually from the outer edge of the matrix. Spectra with less than 500 ion counts for the internal standard were deleted. The laser attenuation was set to produce ca. 64-70 μJ pulses (manufacturers specifications). The threshold for detectable signal under these conditions was ca. 45 μJ/pulse. Spectra were 3-point filtered to improve s/n, and a mass range of 250 mass units covering the main peak and potential adduct peaks was integrated for both analyte and standard peaks. Ratios between analyte and internal standard peak areas were plotted (mean ± SD, Figure 1b).
  • 23
    • 0011921440 scopus 로고    scopus 로고
    • note
    • 15


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.