Adaptation of inverse PCR to generate an internal deletion

BioTechniques

Volumn 26, Issue 4, 1999, Pages 639-641

  Xu, Yanfei ^a   Gong, Zhiyuan ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

GENE DELETION; NONHUMAN; POLYMERASE CHAIN REACTION; PROMOTER REGION; SHORT SURVEY; ZEBRA FISH;

DANIO RERIO;

EID: 0032906880     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99264bm10     Document Type: Short Survey

References (6)

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3. S.Imai, V., Y. Matsushima, T. Sugimura and M. Terada. 1991. A simple and rapid method for generating a deletion by PCR. Nucleic Acids'Res. 79:2785.
4. Luckow, B. and G. Schutz. 1987. CAT constructions with multiple unique restriction sites for the functional analysis of eukaryotic promoters and regulatory elements. Nucleic Acids Res. 75:5490.
5. Ray, F.A. and J.A. Nickoloff. 1992. Site-specific mutagenesis of almost any plasmid using a PCR-based version of unique site elimination. BioTechniques 75:342-348.
6. Xu, Y., J. He, H.L. Tian, C.H. Chan, J. Liao, T. Yan, TJ. Lam and Z. Gong. 1999. Fast skeletal muscle specific expression of a zebrafish myosin light chain 2 gene and characterization of its promoter by direct injection into skeletal muscle. DNA Cell Biol. 75:85-95.