Extensibility of isoforms of cardiac titin: Variation in contour length of molecular subsegments provides a basis for cellular passive stiffness diversity

Biophysical Journal

Volumn 79, Issue 6, 2000, Pages 3226-3234

  Trombitás, Karoly ^a   Redkar, Alka ^a   Centner, Thomas ^b   Wu, Yiming ^a   Labeit, Siegfried ^c   Granzier, Henk ^a  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CONNECTIN; IMMUNOGLOBULIN; ISOPROTEIN; POLYPEPTIDE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; HEART ATRIUM; HEART MUSCLE CELL; HEART VENTRICLE; HUMAN; HUMAN CELL; NONHUMAN; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN STRUCTURE;

ANIMALIA; BOVINAE; MAMMALIA;

EID: 0033637514     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(00)76555-6     Document Type: Article

References (23)

1. Berri, E., J. Wolff, and M. L. Greaser. 1997. Characterization of the titin PEVK fragment from different species. Biophys. J. 72:189a. (Abstr.).
2. Cazorla, O., A. Freiburg, M. Helmes, T. Centner, M. McNabb, K. Trombitás, S. Labeit, and H. Granzier. 2000. Differential expression of cardiac titin isoforms and modulation of cellular stiffness. Circ. Res. 86:59-67.
3. Fabiato, A., and F. Fabiato. 1978. Myofilament-generated tension oscillations during partial calcium activation, and activation dependence of the sarcomere length-tension relation of skinned cardiac cells. J. Gen. Physiol. 72:667-699.
4. Freiburg, A., K. Trombitás, W. Hell, O. Cazorla, F. Fougerousse, T. Centner, B. Kolmerer, C. Witt, J. Beckmann, C. Gregorio, H. Granzier, and S. Labeit. 2000. Series of exon-skipping events in titin's elastic spring region as the structural basis for myofibrillar elastic diversity. Circ. Res. 86:1114-1121.
5. Granzier, H., M. Helmes, and K. Trombitás. 1996. Nonuniform elasticity of titin in cardiac myocytes: a study using immunoelectron microscopy and cellular mechanics. Biophys. J. 70:430-442.
6. Granzier, H., M. Kellermayer, M. Helmes, and K. Trombitás. 1997. Titin elasticity and mechanism of passive force development in rat cardiac myocytes probed by thin-filament extraction. Biophys. J. 73:2043-2053.
7. Granzier, H. L., and T. C. Irving. 1995. Passive tension in cardiac muscle: contribution of collagen, titin, microtubules, and intermediate filaments. Biophys. J. 68:1027-1044.
8. Granzier, H. L., and K. Wang. 1993. Gel electrophoresis of giant proteins: solubilization and silver-staining of titin and nebulin from single muscle fiber segments. Electrophoresis. 14:56-64.
9. Gregorio, C., H. Granzier, H. Sorimachi, and S. Labeit. 1999. Muscle assembly: a titanic achievement? Curr. Opin. Cell Biol. 11:18-25.
10. Grimm, A., H-L Lin, and B. Grimm. 1980. Left ventricular free wall and intraventricular pressure-sarcomere length distribution. Am. J. Physiol. Heart Circ. Physiol. 239:H101-H107.
11. Helmes, M., K. Trombitás, T. Centner, M. Kellermayer, S. Labeit, A. Linke, and H. Granzier. 1999. Mechanically driven contour-length adjustment in rat cardiac titin's unique N2B sequence: titin is an adjustable spring. Circ. Res. 84:1139-1352.
12. Kellermayer, M. S., S. B. Smith, H. L. Granzier, and C. Bustamante. 1997. Folding-unfolding transitions in single titin molecules characterized with laser tweezers. Science. 276:1112-1116.
13. Kentish, J., H. ter Keurs, L. Ricciardi, J. Bucx, and M. Noble. 1986. Comparison between the sarcomere length-force relations of intact and skinned trabeculae from rat ventricle. Circ. Res. 58:766-768.
14. Labeit, S., and B. Kolmerer. 1995. Titins: giant proteins in charge of muscle ultrastructure and elasticity. Science. 270:293-296.
15. Labeit, S., B. Kolmerer, and W. A. Linke. 1997. The giant protein titin. Emerging roles in physiology and pathophysiology. Circ. Res. 80: 290-294.
16. Linke, W., D. Rudy, T. Centner, M. Gautel, C. Witt, S. Labeit, and C. Gregorio. 1999. I-band titin in cardiac muscle is a three-element molecular spring and is critical for maintaining thin filament structure. J. Cell Biol. 146:631-644.
17. Maruyama, K. 1997. Connectin/titin, giant elastic protein of muscle. FASEB J. 11:341-345.
18. Neuhoff, V., N. Arold, D. Taube, and W. Ehrhardt. 1988. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis. 9:255-262.
19. Trinick, J., and L. Tskhovrebova. 1999. Titin: a molecular control freak. Trends Cell Biol. 9:377-380.
20. Trombitás, K., A. Freiburg, T. Centner, S. Labeit, and H. Granzier. 1999. Molecular dissection of N2B cardiac titin's extensibility. Biophys. J. 77:3189-3196.
21. Trombitás, K., M. Greaser, G. French, and H. Granzier. 1998a. PEVK extension of human soleus muscle titin revealed by immunolabeling with the anti-titin antibody 9D10. J. Struct. Biol. 122:188-196.
22. Trombitás, K., M. Greaser, S. Labeit, J. P. Jin, M. Kellermayer, M. Helmes, and H. Granzier. 1998b. Titin extensibility in situ: entropie elasticity of permanently folded and permanently unfolded molecular segments. J. Cell Biol. 140:853-859.
23. Wang, K. 1996. Titin/connectin and nebulin: giant protein rulers of muscle structure and function. Adv. Biophys. J. 33:123-134.