Molecular dissection of N2B cardiac titin's extensibility

Biophysical Journal

Volumn 77, Issue 6, 1999, Pages 3189-3196

  Trombitás, Karoly ^a   Freiburg, Alexandra ^b   Centner, Thomas ^c   Labeit, Siegfried ^c   Granzier, Henk ^a  

^a WASHINGTON STATE UNIVERSITY   (United States)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CONNECTIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; MOUSE; NONHUMAN; PROTEIN DOMAIN; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

EID: 0032785903     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(99)77149-3     Document Type: Article

References (31)

1. Cantor, C., and S. Schimmel. 1980. The Behavior of Biological Macromolecules. Freeman and Company, San Francisco. 849-1371.
2. Cazorla, O., A. Freburg, M. Helmes, T. Centner, M. McNabb, Y. Wu, K. Trombitás, S. Labeit, and H. Granzier. 2000. Differential expression of cardiac titin isoforms and modulation of cellular stiffness. Circ. Res. In press.
3. Fürst, D. O., M. Osborn, R. Nave, and K. Weber. 1988. The organization of titin filaments in the half-sarcomere revealed by monoclonal antibodies in immunoelectron microscopy: a map of ten nonrepetitive epitopes starting at the Z line extends close to the M line. J. Cell Biol. 106: 1563-1572.
4. Gautel, M., E. Lehtonen, and G. Pietruschka. 1996. Assembly of the cardiac I-band region of titin/connectin: expression of the cardiac-specific region and the structural relation to the elastic element. J. Muscle Res. Cell Motil. 17:449-461.
5. Granzier, H., M. Helmes, and K. Trombitás. 1996. Nonuniform elasticity of titin in cardiac myocytes: a study using immunoelectron microscopy and cellular mechanics. Binphys. J. 70:430-442.
6. Granzier, H. L., and T. C. Irving. 1995. Passive tension in cardiac muscle: contribution of collagen, titin, microtubules, and intermediate filaments. Biophys. J. 68:1027-1044.
7. Greaser, M. L., M. H. Sebestyén J. D. Fritz, and J. A. Wolff. 1996. cDNA sequence of rabbit cardiac titin/connectin. Adv. Biophys. 33:13-25.
8. Gregorio, C., H. Granzier, H. Sorimachi, and S. Labeit. 1999. Muscle assembly: a titanic achievement? Curr. Opin. Cell Biol. 11:18-25.
9. Grimm, A., B. Grimm, H. Lin, R. Parshall, and A. Tichy. 1991. Left ventricular shape-luminal pressure relationship. An open-chest study. Basic Res. Cardiol. 86:378-392.
10. Grimm, A. F., H. L. Lin, and B. R. Grimm. 1980. Left ventricular free wall and intraventricular pressure-sarcomere length distributions. Am. J. Physiol. 239:H101-H107.
11. Helmes, M., K. Trombitás, T. Centner, M. Kellermayer, S. Labeit, A. Linke, and H. Granzier. 1999. Mechanically driven contour-length adjustment in rat cardiac titin's unique N2B sequence: titin is an adjustable spring. Circ. Res. 84:1139-1352.
12. Kellermayer, M. S., S. B. Smith, H. L. Granzier, and C. Bustamante. 1997. Folding-unfolding transitions in single titin molecules characterized with laser tweezers, [see comments] Science. 276:1112-1116.
13. Labeit, S., and B. Kolmerer. 1995. Titins: giant proteins in charge of muscle ultrastructure and elasticity. Science. 270:293-296.
14. Labeit, S., B. Kolmerer, and W. A. Linke. 1997. The giant protein titin. Emerging roles in physiology and pathophysiology. Circ. Res. 80: 290-294.
15. Linke W. A., M. Ivemeyer, N. Olivieri, B. Kolmerer, J. C. Ruegg, and S. Labeit. 1996. Towards a molecular understanding of the elasticity of titin. J. Mol. Biol. 261: 62-71.
16. Linke, W. A., M. Ivemeyer, P. Mundel, M. R. Stockmeier, and B. Kolemer. 1998a. Nature of PEVK-titin elasticity in skeletal muscle. Proc. Natl. Acad. Sci. USA. 95:8052-8057.
17. Linke, W. A., M. R. Stockmeier, M. Ivemeyer, H. Hosser, and P. Mundel. 1998b. Characterizing titin's I-band Ig domain region as an entropic spring. J. Cell Science. 111:1567-1574.
18. MacKenna, D. A., J. H. Omens, and J. W. Covell. 1996. Left ventricular perimysial collagen fibers uncoil rather than stretch during diastolic filling. Basic Res. Cardiol. 91:111-122.
19. Maruyama, K. 1997. Connectin/titin, giant elastic protein of muscle. FASEB J. 11:341-345.
20. Rief, M., M. Gautel, F. Oesterhelt, J. M. Fernandez, and H. E. Gaub. 1997. Reversible unfolding of individual titin immunoglobulin domains by AFM. Science. 276:1109-1112.
21. Rief, M., J. Pascual, M. Saraste, and H. E. Gaub. 1999. Single molecule force spectroscopy of spectrin repeats: low unfolding forces in helix bundles. J. Mol. Biol. 19:553-561.
22. Sebestyén, M., J. Wolff, and M. Greaser. 1995. Characterization of a 5.4 kb cDNA fragment from the Z-line region of rabbit cardiac titin reveals phosphorylation sites for proline-directed kinases. J. Cell Sci. 108: 3029-3037.
23. Siu B., H. Niimura, J. Osborne, C. MacRae, S. Solomon, W. Benson, J. Seidman, and C. Seidman. 1999. Familial dilated cardiomyopathy locus maps to chromosome 2q31. Circulation. 99:1022-1026.
24. Trinick, J. 1996. Titin as a scaffold and spring. Cytoskeleton. Curr. Biol. 6:258-260.
25. Trombitás, K., P. Baatsen, M. Kellermayer, G. Pollack. 1991. Nature and origin of gap filaments in striated muscle. J. Cell Sci. 100:809-814.
26. Trombitás, K., M. Greaser, S. Labeit, J. P. Jin, M. Kellermayer, M. Helmes, and H. Granzier. 1998. Titin extensibility in situ: entropic elasticity of permanently folded and permanently unfolded molecular segments. J. Cell Biol. 140:853-859.
27. Trombitás, K., J. P. Jin, and H. Granzier. 1995. The mechanically active domain of titin in cardiac muscle. Circ. Res. 77:856-861.
28. Tskhovrebova, L., J. Trinick, J. A. Sleep, and R. M. Simmons. 1997. Elasticity and unfolding of single molecules of the giant muscle protein titin. Nature. 387:308-312.
29. Wang, K. 1996. Titin/connectin and nebulin: giant protein rulers of muscle structure and function. Adv. Biophys. J. 33:123-134.
30. Witt, C. C., N. Olivieri, T. Centner, B. Kolmerer, S. Millevoi, J. Morell, D. Labeit, S. Labeit, H. Jockusch, and A. Pastore. 1998. A survey of the primary structure and the interspecies conservation of I-band titin's elastic elements in vertebrates. J. Struct. Biol. 122:206-215.
31. Wolska, B. M., and R. J. Solaro. 1996. Method for isolation of adult mouse cardiac myocytes for studies of contraction and microfluorimetry. Am. J. Physiol. 271:H1250-H1255.