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Volumn 286, Issue 5440, 1999, Pages 741-746

Contact-dependent inhibition of cortical neurite growth mediated by Notch signaling

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; BRAIN CELL; CELL CONTACT; GROWTH INHIBITION; IN VITRO STUDY; MOUSE; NERVE CELL; NERVE CELL DIFFERENTIATION; NERVE FIBER GROWTH; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SIGNAL TRANSDUCTION;

EID: 0033595679     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.286.5440.741     Document Type: Article
Times cited : (506)

References (61)
  • 1
    • 0040003111 scopus 로고
    • MIT Press, Cambridge, MA
    • P. Rakic and P. S. Goldman-Rakic, Eds., Development and Modifiability of the Cerebral Cortex (MIT Press, Cambridge, MA, 1982); T. N. Wiesel, Nature 299, 583 (1982); A. M. Craig and G. Banker, Annu. Rev. Neurosci. 17, 267 (1994); H. W. Horch, A. Kruttgen, S. D. Portbury, L. C. Katz, Neuron 23, 353 (1999).
    • (1982) Development and Modifiability of the Cerebral Cortex
    • Rakic, P.1    Goldman-Rakic, P.S.2
  • 2
    • 0019997224 scopus 로고
    • P. Rakic and P. S. Goldman-Rakic, Eds., Development and Modifiability of the Cerebral Cortex (MIT Press, Cambridge, MA, 1982); T. N. Wiesel, Nature 299, 583 (1982); A. M. Craig and G. Banker, Annu. Rev. Neurosci. 17, 267 (1994); H. W. Horch, A. Kruttgen, S. D. Portbury, L. C. Katz, Neuron 23, 353 (1999).
    • (1982) Nature , vol.299 , pp. 583
    • Wiesel, T.N.1
  • 3
    • 0028316399 scopus 로고
    • P. Rakic and P. S. Goldman-Rakic, Eds., Development and Modifiability of the Cerebral Cortex (MIT Press, Cambridge, MA, 1982); T. N. Wiesel, Nature 299, 583 (1982); A. M. Craig and G. Banker, Annu. Rev. Neurosci. 17, 267 (1994); H. W. Horch, A. Kruttgen, S. D. Portbury, L. C. Katz, Neuron 23, 353 (1999).
    • (1994) Annu. Rev. Neurosci. , vol.17 , pp. 267
    • Craig, A.M.1    Banker, G.2
  • 4
    • 0033153184 scopus 로고    scopus 로고
    • P. Rakic and P. S. Goldman-Rakic, Eds., Development and Modifiability of the Cerebral Cortex (MIT Press, Cambridge, MA, 1982); T. N. Wiesel, Nature 299, 583 (1982); A. M. Craig and G. Banker, Annu. Rev. Neurosci. 17, 267 (1994); H. W. Horch, A. Kruttgen, S. D. Portbury, L. C. Katz, Neuron 23, 353 (1999).
    • (1999) Neuron , vol.23 , pp. 353
    • Horch, H.W.1    Kruttgen, A.2    Portbury, S.D.3    Katz, L.C.4
  • 6
    • 0032525964 scopus 로고    scopus 로고
    • J. Kimble and P. Simpson, Annu. Rev. Cell. Dev. Biol. 13, 333 (1997); I. Greenwald, Genes Dev. 12, 1751 (1998).
    • (1998) Genes Dev. , vol.12 , pp. 1751
    • Greenwald, I.1
  • 10
    • 0032532311 scopus 로고    scopus 로고
    • P. Beatus and U. Lendahl, J. Neurosci. Res. 54, 125 (1998); I. Ahmad, P. Zagouras, S. Artavanis-Tsakonas, Mech. Dev. 53, 73 (1995).
    • (1998) J. Neurosci. Res. , vol.54 , pp. 125
    • Beatus, P.1    Lendahl, U.2
  • 12
    • 0345423815 scopus 로고    scopus 로고
    • note
    • Fixed frozen sections and cells were preincubated in blocking solution [BS; 5% donkey serum, 1% bovine serum albumin, 0.1% glycine, 0.1% L-lysine, 0.4% Triton X-100 in phosphate-buffered saline (PBS)] for 1 hour at room temperature, and left overnight at 4°C in primary antibodies/BS: bTAN20, bhN6D [1:5 (29, 34)]; PGHN [1:1200 (34)]; IC [1:800; F. Logeat et al., Proc. Natl. Acad. Sci. U.S.A. 95, 8108 (1998)]; 88-C (1:2000), J59 [1:1000 (23)]; TS1 [1:5 (35)]; NotchC-20, JaggedC-20 (1:250, Santa Cruz); TuJ1 (1:500, BAbCO); anti-synaptophysin (1:200, Sigma); and anti-BrdU (1:75, BectonDickinson)]. After washing in PBS, samples were incubated with Cy2, Cy3, and Cy5 conjugated secondary antibodies (1:300, Jackson Immunoresearch) for 45 min. Single optical images were collected on a MRC 1024 confocal microscope (Bio-Rad).
  • 13
    • 0344561723 scopus 로고    scopus 로고
    • Supplement Web material is available at www. sciencemag.org/feature/data/1042942.shl.
  • 15
    • 0032543565 scopus 로고    scopus 로고
    • M. Lecourtois and F. Schweisguth, Curr. Biol. 8, 771 (1998); G. Struhl and A. Adachi, Cell 93, 549 (1998).
    • (1998) Cell , vol.93 , pp. 549
    • Struhl, G.1    Adachi, A.2
  • 17
    • 26544434970 scopus 로고    scopus 로고
    • thesis, Yale University
    • N. Šestan, thesis, Yale University (1999).
    • (1999)
    • Šestan, N.1
  • 19
    • 0344130187 scopus 로고    scopus 로고
    • note
    • 2.
  • 22
    • 0344561721 scopus 로고    scopus 로고
    • note
    • The Helios Gene Gun (Bio-Rad) was used to transfect neurons with CMV-based plasmids [phN1 FL, pNotch1 FL, pNotch1 ICD (amino acids 1758 to 2556), pNotch2 FL, pNotch2 ICD (amino acids 1701 to 2471) (24, 34); pnumb, pnumb-like (26); pdeltex, p4xwtCBF1-luc (29); pEGFP-N2 (Clontech); placZ]. DNA was precipitated onto ∼35 mg of 1-μm gold microcarrier. Coprecipitation ratios: 25 μg of pEGFP-N2 1:3 with placZ or 1:1.5:1.5 with other plasmids; and 33 μg of p4xwtCBF1-luc 1:3 with placZ or 1:1:1 with other plasmids, to a total of 100 μg. The DNA was delivered into neurons at ∼100 psi. Neurons were analyzed 48 hours after transfection. Enhanced green fluorescent protein (EGFP) autofluorescence was evenly distributed along the entire neurite. EGFP/lacZ coprecipitation resulted in ∼95% efficiency of coexpression as early as 12 to 15 hours after transfection.
  • 23
    • 0344130185 scopus 로고    scopus 로고
    • note
    • The cells were washed with PBS and lysed in 50 μl of lysis buffer. Cell extract (10 μl) was used to measure either luciferase activity or β-galactosidase activity.
  • 24
    • 0030063909 scopus 로고    scopus 로고
    • J. J.-D. Hsieh et al., Mol. Cell. Biol. 16, 952 (1996); F. M. Lu and S. E. Lux, Proc. Natl. Acad. Sci. U.S.A. 93, 5663 (1996); J. J.-D. Hsieh, D. E. Nofziger, G. Weinmaster, S. D. Hayward, J. Virol. 71, 1938 (1997).
    • (1996) Mol. Cell. Biol. , vol.16 , pp. 952
    • Hsieh, J.J.-D.1
  • 25
    • 0029997061 scopus 로고    scopus 로고
    • J. J.-D. Hsieh et al., Mol. Cell. Biol. 16, 952 (1996); F. M. Lu and S. E. Lux, Proc. Natl. Acad. Sci. U.S.A. 93, 5663 (1996); J. J.-D. Hsieh, D. E. Nofziger, G. Weinmaster, S. D. Hayward, J. Virol. 71, 1938 (1997).
    • (1996) Proc. Natl. Acad. Sci. U.S.A. , vol.93 , pp. 5663
    • Lu, F.M.1    Lux, S.E.2
  • 26
  • 28
    • 0344561720 scopus 로고    scopus 로고
    • note
    • For RT-PCR (Titan System, Roche), 100 ng of total RNA were isolated using TRIZOL (Gibco). Typical PCR cycle parameters were as follows: HES analysis, 5 min at 95°C, 45 s at 58°C and 1.5 min at 72°C for 40 cycles; Delta/Jagged analysis, 30 s at 95°C, 30 s at 58°C, and 1 min at 72°C for 45 cycles. The PCR products were separated on a 1% gel, stained with ethidium bromide, purified, and sequenced. For primers, see (7).
  • 29
    • 0027984312 scopus 로고
    • T. L. Fletcher, P. De Camilli, G. Banker, J. Neurosci. 14, 6695 (1994); A. N. van den Pol, K. Obrietan, A. B. Belousov, Y. Yang, H. C. Heller, J. Comp. Neurol. 399, 541 (1998).
    • (1994) J. Neurosci. , vol.14 , pp. 6695
    • Fletcher, T.L.1    De Camilli, P.2    Banker, G.3
  • 31
    • 0344130183 scopus 로고    scopus 로고
    • note
    • Randomly selected neurons (n > 6000) were imaged and coded to conceal their identity during the measuring (24). Neurites longer than 10 μm were traced and counted as branches. Statistical significance was determined by t test Branching of neurites is presented as branching index (number of branches divided by mean neurite length per neuron) analyzed at 9 DIV, which elucidates the different incidence of branching between control lacZ-transfected neurons and other transfected neurons (lacZ = 100%). LD cultures: Notch1 ICD = 144.5 ± 9.3% (P = 0.003), Notch2 ICD = 157.8 ± 12.5% (P = 0.003). HD cultures: Nmb = 43.0 ± 1.3% (P = 0.004), Nbl = 31.3 ± 5.6% (P < 0.0003), Dx = 24.0 ± 5.1% (P < 0.000006).
  • 32
    • 0031785161 scopus 로고    scopus 로고
    • S. Jarriault et al., Mol. Cell. Biol. 18, 7423 (1998); L. Li et al., Immunity 8, 43 (1998).
    • (1998) Mol. Cell. Biol. , vol.18 , pp. 7423
    • Jarriault, S.1
  • 33
    • 0031785161 scopus 로고    scopus 로고
    • S. Jarriault et al., Mol. Cell. Biol. 18, 7423 (1998); L. Li et al., Immunity 8, 43 (1998).
    • (1998) Immunity , vol.8 , pp. 43
    • L, L.1
  • 35
    • 0032126751 scopus 로고    scopus 로고
    • S. Wang et al., Neuron 21, 63 (1998).
    • (1998) Neuron , vol.21 , pp. 63
    • Wang, S.1
  • 36
    • 0032923759 scopus 로고    scopus 로고
    • H. Qi et al., Science 283, 91 (1999).
    • (1999) Science , vol.283 , pp. 91
    • Qi, H.1
  • 37
    • 0344561718 scopus 로고    scopus 로고
    • note
    • ECmyc, amino acids 1 to 1046 (22)] and selected with G-418 (0.8 mg/ml) in 10-cm plates. At least two separate cell pools were generated for each plasmid and assayed for the expression of transfected genes. For cocuiture assays, 3T3 cells were grown in DMEM/F-12 with NZ (Gibco) and 5% FBS (24) until confluence. Neurons growing on the cover slips (5 and 12 mm) were placed on top of the monolayer, facing the 3T3 cells, and maintained for 20 to 24 hours.
  • 39
    • 0030200217 scopus 로고    scopus 로고
    • W. Zhong, J. N. Feder, M.-M. Jiang, L. Y. Jan, Y. N. Jan, Neuron 17, 43 (1996); W. Zhong, M.-M. Jiang, G. Weinmaster, L. Y. Jan, Y. N. Jan, Development 124, 1887 (1997); Y. Wakamatsu, T. M. Maynard, S. U. Jones, J. A. Weston, Neuron 23, 71 (1999).
    • (1996) Neuron , vol.17 , pp. 43
    • Zhong, W.1    Feder, J.N.2    Jiang, M.-M.3    Jan, L.Y.4    Jan, Y.N.5
  • 40
    • 0031000210 scopus 로고    scopus 로고
    • W. Zhong, J. N. Feder, M.-M. Jiang, L. Y. Jan, Y. N. Jan, Neuron 17, 43 (1996); W. Zhong, M.-M. Jiang, G. Weinmaster, L. Y. Jan, Y. N. Jan, Development 124, 1887 (1997); Y. Wakamatsu, T. M. Maynard, S. U. Jones, J. A. Weston, Neuron 23, 71 (1999).
    • (1997) Development , vol.124 , pp. 1887
    • Zhong, W.1    Jiang, M.-M.2    Weinmaster, G.3    Jan, L.Y.4    Jan, Y.N.5
  • 41
    • 0033136704 scopus 로고    scopus 로고
    • W. Zhong, J. N. Feder, M.-M. Jiang, L. Y. Jan, Y. N. Jan, Neuron 17, 43 (1996); W. Zhong, M.-M. Jiang, G. Weinmaster, L. Y. Jan, Y. N. Jan, Development 124, 1887 (1997); Y. Wakamatsu, T. M. Maynard, S. U. Jones, J. A. Weston, Neuron 23, 71 (1999).
    • (1999) Neuron , vol.23 , pp. 71
    • Wakamatsu, Y.1    Maynard, T.M.2    Jones, S.U.3    Weston, J.A.4
  • 44
    • 0015173687 scopus 로고
    • G. A. Dunn, J. Comp. Neurol. 143, 491 (1971); P. Rakic, ibid. 146, 335 (1972); V. H. Perry and R. Linden, Nature 297, 683 (1982).
    • (1971) J. Comp. Neurol. , vol.143 , pp. 491
    • Dunn, G.A.1
  • 45
    • 0015420488 scopus 로고
    • G. A. Dunn, J. Comp. Neurol. 143, 491 (1971); P. Rakic, ibid. 146, 335 (1972); V. H. Perry and R. Linden, Nature 297, 683 (1982).
    • (1972) J. Comp. Neurol. , vol.146 , pp. 335
    • Rakic, P.1
  • 46
    • 0019973461 scopus 로고
    • G. A. Dunn, J. Comp. Neurol. 143, 491 (1971); P. Rakic, ibid. 146, 335 (1972); V. H. Perry and R. Linden, Nature 297, 683 (1982).
    • (1982) Nature , vol.297 , pp. 683
    • Perry, V.H.1    Linden, R.2
  • 47
    • 0032055258 scopus 로고    scopus 로고
    • E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
    • (1998) Neuron , vol.20 , pp. 667
    • Giniger, E.1
  • 48
    • 0031758969 scopus 로고    scopus 로고
    • E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
    • (1998) Mol. Cell. Neurosci. , vol.12 , pp. 281
    • Olson, E.C.1
  • 49
    • 0031801882 scopus 로고    scopus 로고
    • E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
    • (1998) Hum. Mol. Genet. , vol.7 , pp. 1161
    • Yavari, R.1    Adida, C.2    Bray-Ward, P.3    Brines, M.4    Xu, T.5
  • 50
    • 0032771052 scopus 로고    scopus 로고
    • E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
    • (1999) Neuroscience , vol.93 , pp. 433
    • Berezovska, O.1
  • 51
    • 0033135780 scopus 로고    scopus 로고
    • E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
    • (1999) J. Neurosci. Res. , vol.56 , pp. 229
    • Castella, P.1    Wagner, J.A.2    Caudy, M.3
  • 53
    • 0033013543 scopus 로고    scopus 로고
    • O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
    • (1999) Mol. Brain Res. , vol.69 , pp. 273
    • Berezovska, O.1
  • 54
    • 0033535504 scopus 로고    scopus 로고
    • O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
    • (1999) Nature , vol.398 , pp. 518
    • De Strooper, B.1
  • 55
    • 0033536072 scopus 로고    scopus 로고
    • O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
    • (1999) Proc. Natl. Acad. Sci. U.S.A. , vol.96 , pp. 6959
    • Song, W.1
  • 56
    • 0033535508 scopus 로고    scopus 로고
    • O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
    • (1999) Nature , vol.398 , pp. 522
    • Struhl, G.1    Greenwald, I.2
  • 57
    • 0033535555 scopus 로고    scopus 로고
    • O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
    • Nature , pp. 525
    • Ye, Y.1    Lukinova, N.2    Fortini, M.3
  • 60
    • 0344561714 scopus 로고    scopus 로고
    • note
    • Bromodeoxyuridine (BrdU) was administered by intraperitoneal injections (50 mg/kg body weight). The morning of vaginal plug was designated EO.
  • 61
    • 0344561713 scopus 로고    scopus 로고
    • note
    • We thank R. Mann for making human Delta and Jagged plasmids; G. Weinmaster and C Hicks for 88C and J59 antibodies; A. Israel for IC antibodies; E. Mitsiadis for mouse Notch and Jagged plasmids; J. McGlade for Numb and Numb-like plasmids; T. Kadesch for CBF1-luc and Deltex plasmids; E. Nestler and J. Chen for use of the luminometer; and M. Ding, M. Donoghue, T. Haydar, and P. Mellman for technical assistance. Supported by NIH grants NS14841 (P.R.) and NS26084 (S.A.-T.).


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