-
1
-
-
0040003111
-
-
MIT Press, Cambridge, MA
-
P. Rakic and P. S. Goldman-Rakic, Eds., Development and Modifiability of the Cerebral Cortex (MIT Press, Cambridge, MA, 1982); T. N. Wiesel, Nature 299, 583 (1982); A. M. Craig and G. Banker, Annu. Rev. Neurosci. 17, 267 (1994); H. W. Horch, A. Kruttgen, S. D. Portbury, L. C. Katz, Neuron 23, 353 (1999).
-
(1982)
Development and Modifiability of the Cerebral Cortex
-
-
Rakic, P.1
Goldman-Rakic, P.S.2
-
2
-
-
0019997224
-
-
P. Rakic and P. S. Goldman-Rakic, Eds., Development and Modifiability of the Cerebral Cortex (MIT Press, Cambridge, MA, 1982); T. N. Wiesel, Nature 299, 583 (1982); A. M. Craig and G. Banker, Annu. Rev. Neurosci. 17, 267 (1994); H. W. Horch, A. Kruttgen, S. D. Portbury, L. C. Katz, Neuron 23, 353 (1999).
-
(1982)
Nature
, vol.299
, pp. 583
-
-
Wiesel, T.N.1
-
3
-
-
0028316399
-
-
P. Rakic and P. S. Goldman-Rakic, Eds., Development and Modifiability of the Cerebral Cortex (MIT Press, Cambridge, MA, 1982); T. N. Wiesel, Nature 299, 583 (1982); A. M. Craig and G. Banker, Annu. Rev. Neurosci. 17, 267 (1994); H. W. Horch, A. Kruttgen, S. D. Portbury, L. C. Katz, Neuron 23, 353 (1999).
-
(1994)
Annu. Rev. Neurosci.
, vol.17
, pp. 267
-
-
Craig, A.M.1
Banker, G.2
-
4
-
-
0033153184
-
-
P. Rakic and P. S. Goldman-Rakic, Eds., Development and Modifiability of the Cerebral Cortex (MIT Press, Cambridge, MA, 1982); T. N. Wiesel, Nature 299, 583 (1982); A. M. Craig and G. Banker, Annu. Rev. Neurosci. 17, 267 (1994); H. W. Horch, A. Kruttgen, S. D. Portbury, L. C. Katz, Neuron 23, 353 (1999).
-
(1999)
Neuron
, vol.23
, pp. 353
-
-
Horch, H.W.1
Kruttgen, A.2
Portbury, S.D.3
Katz, L.C.4
-
6
-
-
0032525964
-
-
J. Kimble and P. Simpson, Annu. Rev. Cell. Dev. Biol. 13, 333 (1997); I. Greenwald, Genes Dev. 12, 1751 (1998).
-
(1998)
Genes Dev.
, vol.12
, pp. 1751
-
-
Greenwald, I.1
-
10
-
-
0032532311
-
-
P. Beatus and U. Lendahl, J. Neurosci. Res. 54, 125 (1998); I. Ahmad, P. Zagouras, S. Artavanis-Tsakonas, Mech. Dev. 53, 73 (1995).
-
(1998)
J. Neurosci. Res.
, vol.54
, pp. 125
-
-
Beatus, P.1
Lendahl, U.2
-
11
-
-
0029143840
-
-
P. Beatus and U. Lendahl, J. Neurosci. Res. 54, 125 (1998); I. Ahmad, P. Zagouras, S. Artavanis-Tsakonas, Mech. Dev. 53, 73 (1995).
-
(1995)
Mech. Dev.
, vol.53
, pp. 73
-
-
Ahmad, I.1
Zagouras, P.2
Artavanis-Tsakonas, S.3
-
12
-
-
0345423815
-
-
note
-
Fixed frozen sections and cells were preincubated in blocking solution [BS; 5% donkey serum, 1% bovine serum albumin, 0.1% glycine, 0.1% L-lysine, 0.4% Triton X-100 in phosphate-buffered saline (PBS)] for 1 hour at room temperature, and left overnight at 4°C in primary antibodies/BS: bTAN20, bhN6D [1:5 (29, 34)]; PGHN [1:1200 (34)]; IC [1:800; F. Logeat et al., Proc. Natl. Acad. Sci. U.S.A. 95, 8108 (1998)]; 88-C (1:2000), J59 [1:1000 (23)]; TS1 [1:5 (35)]; NotchC-20, JaggedC-20 (1:250, Santa Cruz); TuJ1 (1:500, BAbCO); anti-synaptophysin (1:200, Sigma); and anti-BrdU (1:75, BectonDickinson)]. After washing in PBS, samples were incubated with Cy2, Cy3, and Cy5 conjugated secondary antibodies (1:300, Jackson Immunoresearch) for 45 min. Single optical images were collected on a MRC 1024 confocal microscope (Bio-Rad).
-
-
-
-
13
-
-
0344561723
-
-
Supplement Web material is available at www. sciencemag.org/feature/data/1042942.shl.
-
-
-
-
15
-
-
0032543565
-
-
M. Lecourtois and F. Schweisguth, Curr. Biol. 8, 771 (1998); G. Struhl and A. Adachi, Cell 93, 549 (1998).
-
(1998)
Cell
, vol.93
, pp. 549
-
-
Struhl, G.1
Adachi, A.2
-
17
-
-
26544434970
-
-
thesis, Yale University
-
N. Šestan, thesis, Yale University (1999).
-
(1999)
-
-
Šestan, N.1
-
19
-
-
0344130187
-
-
note
-
2.
-
-
-
-
20
-
-
0039411073
-
-
C. Akazawa, Y. Sasai, S. Nakanishi, R. Kageyama, J. Biol. Chem. 267, 2187 (1992); Y. Sasai, R. Kageyama, Y. Tagawa, R. Shigemoto, S. Nakanishi, Genes Dev. 6, 2620 (1992).
-
(1992)
J. Biol. Chem.
, vol.267
, pp. 2187
-
-
Akazawa, C.1
Sasai, Y.2
Nakanishi, S.3
Kageyama, R.4
-
21
-
-
0027083415
-
-
C. Akazawa, Y. Sasai, S. Nakanishi, R. Kageyama, J. Biol. Chem. 267, 2187 (1992); Y. Sasai, R. Kageyama, Y. Tagawa, R. Shigemoto, S. Nakanishi, Genes Dev. 6, 2620 (1992).
-
(1992)
Genes Dev.
, vol.6
, pp. 2620
-
-
Sasai, Y.1
Kageyama, R.2
Tagawa, Y.3
Shigemoto, R.4
Nakanishi, S.5
-
22
-
-
0344561721
-
-
note
-
The Helios Gene Gun (Bio-Rad) was used to transfect neurons with CMV-based plasmids [phN1 FL, pNotch1 FL, pNotch1 ICD (amino acids 1758 to 2556), pNotch2 FL, pNotch2 ICD (amino acids 1701 to 2471) (24, 34); pnumb, pnumb-like (26); pdeltex, p4xwtCBF1-luc (29); pEGFP-N2 (Clontech); placZ]. DNA was precipitated onto ∼35 mg of 1-μm gold microcarrier. Coprecipitation ratios: 25 μg of pEGFP-N2 1:3 with placZ or 1:1.5:1.5 with other plasmids; and 33 μg of p4xwtCBF1-luc 1:3 with placZ or 1:1:1 with other plasmids, to a total of 100 μg. The DNA was delivered into neurons at ∼100 psi. Neurons were analyzed 48 hours after transfection. Enhanced green fluorescent protein (EGFP) autofluorescence was evenly distributed along the entire neurite. EGFP/lacZ coprecipitation resulted in ∼95% efficiency of coexpression as early as 12 to 15 hours after transfection.
-
-
-
-
23
-
-
0344130185
-
-
note
-
The cells were washed with PBS and lysed in 50 μl of lysis buffer. Cell extract (10 μl) was used to measure either luciferase activity or β-galactosidase activity.
-
-
-
-
24
-
-
0030063909
-
-
J. J.-D. Hsieh et al., Mol. Cell. Biol. 16, 952 (1996); F. M. Lu and S. E. Lux, Proc. Natl. Acad. Sci. U.S.A. 93, 5663 (1996); J. J.-D. Hsieh, D. E. Nofziger, G. Weinmaster, S. D. Hayward, J. Virol. 71, 1938 (1997).
-
(1996)
Mol. Cell. Biol.
, vol.16
, pp. 952
-
-
Hsieh, J.J.-D.1
-
25
-
-
0029997061
-
-
J. J.-D. Hsieh et al., Mol. Cell. Biol. 16, 952 (1996); F. M. Lu and S. E. Lux, Proc. Natl. Acad. Sci. U.S.A. 93, 5663 (1996); J. J.-D. Hsieh, D. E. Nofziger, G. Weinmaster, S. D. Hayward, J. Virol. 71, 1938 (1997).
-
(1996)
Proc. Natl. Acad. Sci. U.S.A.
, vol.93
, pp. 5663
-
-
Lu, F.M.1
Lux, S.E.2
-
26
-
-
0031047971
-
-
J. J.-D. Hsieh et al., Mol. Cell. Biol. 16, 952 (1996); F. M. Lu and S. E. Lux, Proc. Natl. Acad. Sci. U.S.A. 93, 5663 (1996); J. J.-D. Hsieh, D. E. Nofziger, G. Weinmaster, S. D. Hayward, J. Virol. 71, 1938 (1997).
-
(1997)
J. Virol.
, vol.71
, pp. 1938
-
-
Hsieh, J.J.-D.1
Nofziger, D.E.2
Weinmaster, G.3
Hayward, S.D.4
-
27
-
-
0032915458
-
-
D. Nofziger, A. Miyamoto, K. M. Lyons, G. Weinmaster, Development 126, 1689 (1999).
-
(1999)
Development
, vol.126
, pp. 1689
-
-
Nofziger, D.1
Miyamoto, A.2
Lyons, K.M.3
Weinmaster, G.4
-
28
-
-
0344561720
-
-
note
-
For RT-PCR (Titan System, Roche), 100 ng of total RNA were isolated using TRIZOL (Gibco). Typical PCR cycle parameters were as follows: HES analysis, 5 min at 95°C, 45 s at 58°C and 1.5 min at 72°C for 40 cycles; Delta/Jagged analysis, 30 s at 95°C, 30 s at 58°C, and 1 min at 72°C for 45 cycles. The PCR products were separated on a 1% gel, stained with ethidium bromide, purified, and sequenced. For primers, see (7).
-
-
-
-
29
-
-
0027984312
-
-
T. L. Fletcher, P. De Camilli, G. Banker, J. Neurosci. 14, 6695 (1994); A. N. van den Pol, K. Obrietan, A. B. Belousov, Y. Yang, H. C. Heller, J. Comp. Neurol. 399, 541 (1998).
-
(1994)
J. Neurosci.
, vol.14
, pp. 6695
-
-
Fletcher, T.L.1
De Camilli, P.2
Banker, G.3
-
30
-
-
0032487369
-
-
T. L. Fletcher, P. De Camilli, G. Banker, J. Neurosci. 14, 6695 (1994); A. N. van den Pol, K. Obrietan, A. B. Belousov, Y. Yang, H. C. Heller, J. Comp. Neurol. 399, 541 (1998).
-
(1998)
J. Comp. Neurol.
, vol.399
, pp. 541
-
-
Van Den Pol, A.N.1
Obrietan, K.2
Belousov, A.B.3
Yang, Y.4
Heller, H.C.5
-
31
-
-
0344130183
-
-
note
-
Randomly selected neurons (n > 6000) were imaged and coded to conceal their identity during the measuring (24). Neurites longer than 10 μm were traced and counted as branches. Statistical significance was determined by t test Branching of neurites is presented as branching index (number of branches divided by mean neurite length per neuron) analyzed at 9 DIV, which elucidates the different incidence of branching between control lacZ-transfected neurons and other transfected neurons (lacZ = 100%). LD cultures: Notch1 ICD = 144.5 ± 9.3% (P = 0.003), Notch2 ICD = 157.8 ± 12.5% (P = 0.003). HD cultures: Nmb = 43.0 ± 1.3% (P = 0.004), Nbl = 31.3 ± 5.6% (P < 0.0003), Dx = 24.0 ± 5.1% (P < 0.000006).
-
-
-
-
32
-
-
0031785161
-
-
S. Jarriault et al., Mol. Cell. Biol. 18, 7423 (1998); L. Li et al., Immunity 8, 43 (1998).
-
(1998)
Mol. Cell. Biol.
, vol.18
, pp. 7423
-
-
Jarriault, S.1
-
33
-
-
0031785161
-
-
S. Jarriault et al., Mol. Cell. Biol. 18, 7423 (1998); L. Li et al., Immunity 8, 43 (1998).
-
(1998)
Immunity
, vol.8
, pp. 43
-
-
L, L.1
-
35
-
-
0032126751
-
-
S. Wang et al., Neuron 21, 63 (1998).
-
(1998)
Neuron
, vol.21
, pp. 63
-
-
Wang, S.1
-
36
-
-
0032923759
-
-
H. Qi et al., Science 283, 91 (1999).
-
(1999)
Science
, vol.283
, pp. 91
-
-
Qi, H.1
-
37
-
-
0344561718
-
-
note
-
ECmyc, amino acids 1 to 1046 (22)] and selected with G-418 (0.8 mg/ml) in 10-cm plates. At least two separate cell pools were generated for each plasmid and assayed for the expression of transfected genes. For cocuiture assays, 3T3 cells were grown in DMEM/F-12 with NZ (Gibco) and 5% FBS (24) until confluence. Neurons growing on the cover slips (5 and 12 mm) were placed on top of the monolayer, facing the 3T3 cells, and maintained for 20 to 24 hours.
-
-
-
-
39
-
-
0030200217
-
-
W. Zhong, J. N. Feder, M.-M. Jiang, L. Y. Jan, Y. N. Jan, Neuron 17, 43 (1996); W. Zhong, M.-M. Jiang, G. Weinmaster, L. Y. Jan, Y. N. Jan, Development 124, 1887 (1997); Y. Wakamatsu, T. M. Maynard, S. U. Jones, J. A. Weston, Neuron 23, 71 (1999).
-
(1996)
Neuron
, vol.17
, pp. 43
-
-
Zhong, W.1
Feder, J.N.2
Jiang, M.-M.3
Jan, L.Y.4
Jan, Y.N.5
-
40
-
-
0031000210
-
-
W. Zhong, J. N. Feder, M.-M. Jiang, L. Y. Jan, Y. N. Jan, Neuron 17, 43 (1996); W. Zhong, M.-M. Jiang, G. Weinmaster, L. Y. Jan, Y. N. Jan, Development 124, 1887 (1997); Y. Wakamatsu, T. M. Maynard, S. U. Jones, J. A. Weston, Neuron 23, 71 (1999).
-
(1997)
Development
, vol.124
, pp. 1887
-
-
Zhong, W.1
Jiang, M.-M.2
Weinmaster, G.3
Jan, L.Y.4
Jan, Y.N.5
-
41
-
-
0033136704
-
-
W. Zhong, J. N. Feder, M.-M. Jiang, L. Y. Jan, Y. N. Jan, Neuron 17, 43 (1996); W. Zhong, M.-M. Jiang, G. Weinmaster, L. Y. Jan, Y. N. Jan, Development 124, 1887 (1997); Y. Wakamatsu, T. M. Maynard, S. U. Jones, J. A. Weston, Neuron 23, 71 (1999).
-
(1999)
Neuron
, vol.23
, pp. 71
-
-
Wakamatsu, Y.1
Maynard, T.M.2
Jones, S.U.3
Weston, J.A.4
-
44
-
-
0015173687
-
-
G. A. Dunn, J. Comp. Neurol. 143, 491 (1971); P. Rakic, ibid. 146, 335 (1972); V. H. Perry and R. Linden, Nature 297, 683 (1982).
-
(1971)
J. Comp. Neurol.
, vol.143
, pp. 491
-
-
Dunn, G.A.1
-
45
-
-
0015420488
-
-
G. A. Dunn, J. Comp. Neurol. 143, 491 (1971); P. Rakic, ibid. 146, 335 (1972); V. H. Perry and R. Linden, Nature 297, 683 (1982).
-
(1972)
J. Comp. Neurol.
, vol.146
, pp. 335
-
-
Rakic, P.1
-
46
-
-
0019973461
-
-
G. A. Dunn, J. Comp. Neurol. 143, 491 (1971); P. Rakic, ibid. 146, 335 (1972); V. H. Perry and R. Linden, Nature 297, 683 (1982).
-
(1982)
Nature
, vol.297
, pp. 683
-
-
Perry, V.H.1
Linden, R.2
-
47
-
-
0032055258
-
-
E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
-
(1998)
Neuron
, vol.20
, pp. 667
-
-
Giniger, E.1
-
48
-
-
0031758969
-
-
E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
-
(1998)
Mol. Cell. Neurosci.
, vol.12
, pp. 281
-
-
Olson, E.C.1
-
49
-
-
0031801882
-
-
E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
-
(1998)
Hum. Mol. Genet.
, vol.7
, pp. 1161
-
-
Yavari, R.1
Adida, C.2
Bray-Ward, P.3
Brines, M.4
Xu, T.5
-
50
-
-
0032771052
-
-
E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
-
(1999)
Neuroscience
, vol.93
, pp. 433
-
-
Berezovska, O.1
-
51
-
-
0033135780
-
-
E. Giniger, Neuron 20, 667 (1998); E. C. Olson et al., Mol. Cell. Neurosci. 12, 281 (1998); R. Yavari, C. Adida, P. Bray-Ward, M. Brines, T. Xu, Hum. Mol. Genet. 7, 1161 (1998); O. Berezovska et al., Neuroscience 93, 433 (1999); P. Castella, J. A. Wagner, M. Caudy, J. Neurosci. Res. 56, 229 (1999).
-
(1999)
J. Neurosci. Res.
, vol.56
, pp. 229
-
-
Castella, P.1
Wagner, J.A.2
Caudy, M.3
-
53
-
-
0033013543
-
-
O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
-
(1999)
Mol. Brain Res.
, vol.69
, pp. 273
-
-
Berezovska, O.1
-
54
-
-
0033535504
-
-
O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
-
(1999)
Nature
, vol.398
, pp. 518
-
-
De Strooper, B.1
-
55
-
-
0033536072
-
-
O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
-
(1999)
Proc. Natl. Acad. Sci. U.S.A.
, vol.96
, pp. 6959
-
-
Song, W.1
-
56
-
-
0033535508
-
-
O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
-
(1999)
Nature
, vol.398
, pp. 522
-
-
Struhl, G.1
Greenwald, I.2
-
57
-
-
0033535555
-
-
O. Berezovska et al., Mol. Brain Res. 69, 273 (1999); B. De Strooper et al., Nature 398, 518 (1999); W. Song et al., Proc. Natl. Acad. Sci. U.S.A. 96, 6959 (1999); G. Struhl and I. Greenwald, Nature 398, 522 (1999); Y. Ye, N. Lukinova, M. Fortini, ibid., p. 525.
-
Nature
, pp. 525
-
-
Ye, Y.1
Lukinova, N.2
Fortini, M.3
-
58
-
-
0029078323
-
-
P. Zagouras, S. Stifani, C. M. Blaumueller, M. L. Carcangiu, S. Artavanis-Tsakonas, Proc. Natl. Acad. Sci. U.S.A. 92, 6414 (1995).
-
(1995)
Proc. Natl. Acad. Sci. U.S.A.
, vol.92
, pp. 6414
-
-
Zagouras, P.1
Stifani, S.2
Blaumueller, C.M.3
Carcangiu, M.L.4
Artavanis-Tsakonas, S.5
-
60
-
-
0344561714
-
-
note
-
Bromodeoxyuridine (BrdU) was administered by intraperitoneal injections (50 mg/kg body weight). The morning of vaginal plug was designated EO.
-
-
-
-
61
-
-
0344561713
-
-
note
-
We thank R. Mann for making human Delta and Jagged plasmids; G. Weinmaster and C Hicks for 88C and J59 antibodies; A. Israel for IC antibodies; E. Mitsiadis for mouse Notch and Jagged plasmids; J. McGlade for Numb and Numb-like plasmids; T. Kadesch for CBF1-luc and Deltex plasmids; E. Nestler and J. Chen for use of the luminometer; and M. Ding, M. Donoghue, T. Haydar, and P. Mellman for technical assistance. Supported by NIH grants NS14841 (P.R.) and NS26084 (S.A.-T.).
-
-
-
|