Phosphorylation of vasodilator-stimulated phosphoprotein: A consequence of nitric oxide- and cGMP-mediated signal transduction in brain capillary endothelial cells and astrocytes

Molecular Brain Research

Volumn 67, Issue 2, 1999, Pages 258-266

  Sporbert, Anje ^a   Mertsch, Katharina ^a   Smolenski, Albert ^c   Haseloff, Reiner F ^a   Schönfelder, Gilbert ^b   Paul, Martin ^b   Ruth, Peter ^d   Walter, Ulrich ^c   Blasig, Ingolf E ^a  

^a LEIBNIZ INSTITUT FÜR MOLEKULARE PHARMAKOLOGIE   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^c UNIVERSITY HOSPITAL WÜRZBURG   (Germany)

^d TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Astrocyte; Brain capillary endothelial cell; cGMP; cGMP dependent protein kinase; Nitric oxide; Vasodilator stimulated phosphoprotein

Indexed keywords

CYCLIC GMP; NITRIC OXIDE; PHOSPHOPROTEIN;

ANIMAL CELL; ARTICLE; ASTROCYTE; BLOOD BRAIN BARRIER; BRAIN CAPILLARY; CAPILLARY ENDOTHELIUM; CONTROLLED STUDY; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION;

ANIMALS; ASTROCYTES; BLOOD PROTEINS; BLOOD-BRAIN BARRIER; CAPILLARIES; CELL ADHESION MOLECULES; CELL COMMUNICATION; CELLS, CULTURED; CYCLIC GMP; CYCLIC GMP-DEPENDENT PROTEIN KINASES; ENDOTHELIUM, VASCULAR; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GUANYLATE CYCLASE; MICROFILAMENT PROTEINS; NITRATES; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; NITRIC OXIDE SYNTHASE TYPE II; NITRITES; PHOSPHOPROTEINS; PHOSPHORYLATION; RATS; RATS, WISTAR; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SIGNAL TRANSDUCTION;

ANIMALIA;

EID: 0033586662     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-328X(99)00067-4     Document Type: Article

References (42)

1. Abbott N.J., Hughes C.C.W., Revest P.A., Greenwood J. Development and characterization of a rat brain capillary endothelial culture: towards an in vitro blood-brain barrier. J. Cell Sci. 103:1992;23-27.
2. 2+/calmodulin-dependent cGMP phosphodiesterase activity in granule neurons and astrocytes from rat cerebellum. Eur. J. Pharmacol. 323:1997;119-125.
3. Arthur F.E., Shivers R.R., Bowman P.D. Astrocyte-mediated induction of tight junctions in brain capillary endothelium: an efficient in vitro model. Dev. Brain Res. 36:1987;155-159.
4. Baltrons M.A., Saadoun S., Agullo L., Garcia A. Regulation by calcium of the nitric oxide/cGMP system in cerebellar granule cells and astroglia in culture. J. Neurosci. Res. 49:1997;333-341.
5. Blasig I.E., Sporbert A., Utepbergenov D.I., Schröter M.L., Mertsch K., Haseloff R.F. Cytokine- and hypoxia-induced lipid peroxidation in astrocytes. Int. J. Clin. Pharmacol. Ther. 35:1998;112-113.
6. Bull H.A., Hothersall J., Chowdhury N., Cohen J., Dowd P.M. Neuropeptides induce release of nitric oxide from human dermal microvascular endothelial cells. J. Invest. Dermatol. 106:1996;655-660.
7. Chakravarthy U., Stitt A.W., McNally J., Bailie J.R., Hoey E.M., Duprex P. Nitric oxide synthase activity and expression in retinal capillary endothelial cells and pericytes. Curr. Eye Res. 14:1995;285-294.
8. Draijer R., Vaandrager A.B., Nolte C., de Jonge H., Walter U., Hindsbergh V.W.M. Expression of cGMP-dependent protein kinase I and phosphorylation of its substrate VASP in human endothelial cells of different origin. Circ. Res. 77:1995;897-905.
9. Durieux-Trautmann O., Federici C., Creminon C., Foignant-Chaverot N., Roux F., Claire M., Strosberg A.D., Couraud P.O. Nitric oxide and endothelin secretion by brain microvessel endothelial cells: regulation by cyclic nucleotides. J. Cell. Physiol. 155:1993;104-111.
10. Frey A., Meckelein B., Weiler-Güttler H., Möckel B., Flach R., Gassen H.G. Pericytes of the brain microvasculature express gamma-glutamyltranspeptidase. Eur. J. Pharm. 202:1992;421-429.
11. Giese H., Mertsch K., Blasig I.E. Effect of MK-801 and U83836E on a porcine brain capillary endothelial cell barrier during hypoxia. Neurosci. Lett. 191:1995;1-4.
12. Goldstein G.W., Betz A.L. Recent advances in understanding brain capillary function. Ann. Neurol. 14:1993;389-395.
13. Halbrügge M., Friedrich C., Eigenthaler M., Schanzenbächer P., Walter U. Stoichiometric and reversible phosphorylation of a 46-kDa protein in human platelets in response to cGMP- and cAMP-elevating vasodilators. J. Biol. Chem. 265:1990;3088-3093.
14. Hofmann F., Dostmann W., Keilbach A., Landgraf W., Ruth P. Structure and physiological role of cGMP-dependent protein kinase. Biochem. Biophys. Acta. 1135:1992;51-60.
15. S.J. Hopkins, N.J. Rothwell, Cytokines and the nervous system I: expression and recognition: II. Action and mechanisms of action, TINS 18 (1995) 83-88 and 130-136.
16. Janzer R.C., Raff M.C. Astrocytes induce blood-brain barrier properties in endothelial cells. Nature. 325:1987;253-257.
17. Keilbach A., Ruth P., Hofmann F. Detection of cGMP dependent protein kinase isozymes by specific antibodies. Eur. J. Biochem. 208:1992;467-473.
18. Koga S., Morries S., Ogawa S., Liao H., Bilezikian J.P., Chen G., Thompson W.J., Ashikaga T., Brett J., Stern D.M., Pinsky D.J. TNF modulates endothelial properties by decreasing cAMP. Am. J. Physiol. 37:1995;C1104-C1113.
19. Kubes P. Nitric oxide induced microvascular permeability alterations: a regulatory role for cGMP. Am. J. Physiol. 265:1993;H1909-H1915.
20. Kugler P., Drenckhahn D. Astrocytes and Bergmann glia as important site of nitric oxide synthase I. Glia. 16(1):1996;65-173.
21. Lofton C.E., Newman W.H., Curie M.G. Atrial natriuretic peptide regulation of endothelial permeability is mediated by cGMP. Biochem. Biophys. Res. Commun. 172:1990;793-799.
22. Lohmann S.M., Vaandrager A.B., Smolenski A., Walter U., de Jonge H.R. Distinct and specific functions of cGMP-dependent protein kinases. TIBS. 22:1997;307-312.
23. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193:1951;265-275.
24. Meyer J., Rauh J., Galla H.J. The susceptibility of cerebral endothelial cells to astroglial induction of blood-brain barrier enzymes depends on their proliferative state. J. Neurochem. 57:1991;1971-1977.
25. Papapetroulos A., Marczin N., Mora G.F., Milici A., Murad F., Catravas C.D. Regulation of vascular smooth muscle soluble guanylate cyclase activity, mRNA, and protein by cAMP-elevating agents. Hypertension. 26:1996;696-704.
26. Prado R., Watson B.D., Kuluz J., Dietrich D.W. Endothelium-derived nitric oxide synthase inhibition: effects on cerebral blood-flow, pial artery diameter, and vascular morphology in rats. Stroke. 23:1992;1118-1124.
27. Raff T., van der Giet M., Endemann D., Wiederholt T., Paul M. Design and testing of beta-actin primers for RT-PCR that do not co-amplify processed pseudogenes. BioTechniques. 23:1997;456-460.
28. Reinhard M., Giehl K., Abel K., Haffner C., Jarchau T., Hoppe V., Jockusch B.M., Walter U. The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins. EMBO J. 14:1995;1583-1589.
29. Reinhard M., Rdiger M., Jockusch B.M., Walter U. VASP interaction with vinculin: a recurring theme of interactions with proline-rich motifs. FEBS Lett. 399:1996;103-107.
30. M. Reinhard, T. Jarchau, U. Walter, VASP, in: T. Kreis, R. Vale (Eds.), Guidebook to the Cytoskeletal and Motor Proteins, Oxford Univ. Press, Oxford, 1998, in press.
31. Risau W., Engelhardt B., Wekerle H. Immune function of the blood-brain barrier: incomplete presentation of protein (auto-)antigens by a rat brain microvascular endothelium in vitro. J. Cell Biol. 110:1990;1757-1766.
32. Roux F., Durieux-Trautmann O., Chaverot N., Claire M., Mailly P., Bourre I.M., Strosberg A.D., Couraud P.O. Regulation of gamma-glutamyl transpeptidase and alkaline phosphatase activities in immortalized rat brain microvessel endothelial cells. J. Cell. Physiol. 159:1994;101-113.
33. Rubin L.L., Hall D.E., Porte S., Barbu K., Cannon C., Horner H.C., Janatpour N., Liaw C.W., Manning K., Morales J., Tanner L.I., Tommaselli K.J., Bard F. A cell culture model of the blood-brain barrier. J. Cell Biol. 115:1991;1725-1735.
34. Sandberg M., Natarajan V., Ronander I., Kalderon D., Walter U., Lohmann S.M., Jahnsen T. Molecular cloning and predicted full-length amino acid sequence of the type Iβ isozyme of cGMP-dependent protein kinase from human placenta. FEBS Lett. 255:1989;321-329.
35. H.H.H.W. Schmidt, M. Kelm, Determination of nitrite and nitrate by the Griess reaction, in: M. Feelisch, J.S. Stamler (Eds.), Methods in Nitric Oxide Research, Wiley, England, 1996, pp. 491-497.
36. Schönfelder G., John M., Hopp H., Fuhr N., van der Giet M., Paul M. Expression of inducible nitric oxide synthase in placenta of women with gestational diabetes. FASEB J. 10:1996;777-781.
37. Shukla A., Dikshit M., Scrimal R.C. Nitric oxide dependent blood-brain barrier alterations in the rat brain. Experientia. 52:1996;136-140.
38. Smolenski A., Bachmann C., Reinhard K., Hoenig-Liedl P., Jarchau T., Hoschuetzky H., Walter U. Analysis and regulation of vasodilator-stimulated phosphoprotein Serin 239 phosphorylation in vitro and in intact cells using phosphospecific monoclonal antibody. J. Biol. Chem. 273:1998;15. in press.
39. Staddon J.M., Rubin L.L. Cell adhesion, cell junctions and the blood-brain barrier. Curr. Opin. Neurobiol. 6:1996;622-627.
40. Suttorp N., Hippenstiel S., Fuhrmann M., Krull M., Podzuweit T. Role of nitric oxide and phosphodiesterase II for reduction of endothelial hyperpermeability. Am. J. Physiol. 270:1996;C778-C785.
41. D.I. Utepbergenov, K. Mertsch, A. Sporbert, K. Tenz, M. Paul, R.F. Haseloff, I.E. Blasig, Nitric oxide protects blood-brain barrier in vitro from hypoxia/reoxygenation-mediated injury, FEBS Lett. (1998).
42. 2+-dependent NO-production from CNS-cells in culture measured by NO-nitrite/ozone chemoluminescence. Brain Res. 748:1997;1-11.