메뉴 건너뛰기




Volumn 285, Issue 5435, 1999, Pages 1911-1914

Coincident induction of long-term facilitation in Aplysia: Cooperativity between cell bodies and remote synapses

Author keywords

[No Author keywords available]

Indexed keywords

SEROTONIN;

EID: 0033578658     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5435.1911     Document Type: Article
Times cited : (76)

References (36)
  • 1
    • 0027476024 scopus 로고
    • T. V. P. Bliss and G. L. Collingridge, Nature 361, 31 (1993); Y.-Y. Huang, X. C. Li, E. R. Kandel, Cell 79, 69 (1994); M. Krug, B. Lossner, T. Ott, Br. Res. Bull. 13, 39 (1984); H. Matthies and K. G. Reymann, Neuroreport 4, 712 (1993); P. V. Nguyen, T. Abel, E. R. Kandel, Science 265, 1104 (1994).
    • (1993) Nature , vol.361 , pp. 31
    • Bliss, T.V.P.1    Collingridge, G.L.2
  • 2
    • 0028170035 scopus 로고
    • T. V. P. Bliss and G. L. Collingridge, Nature 361, 31 (1993); Y.-Y. Huang, X. C. Li, E. R. Kandel, Cell 79, 69 (1994); M. Krug, B. Lossner, T. Ott, Br. Res. Bull. 13, 39 (1984); H. Matthies and K. G. Reymann, Neuroreport 4, 712 (1993); P. V. Nguyen, T. Abel, E. R. Kandel, Science 265, 1104 (1994).
    • (1994) Cell , vol.79 , pp. 69
    • Huang, Y.-Y.1    Li, X.C.2    Kandel, E.R.3
  • 3
    • 0021222306 scopus 로고
    • T. V. P. Bliss and G. L. Collingridge, Nature 361, 31 (1993); Y.-Y. Huang, X. C. Li, E. R. Kandel, Cell 79, 69 (1994); M. Krug, B. Lossner, T. Ott, Br. Res. Bull. 13, 39 (1984); H. Matthies and K. G. Reymann, Neuroreport 4, 712 (1993); P. V. Nguyen, T. Abel, E. R. Kandel, Science 265, 1104 (1994).
    • (1984) Br. Res. Bull. , vol.13 , pp. 39
    • Krug, M.1    Lossner, B.2    Ott, T.3
  • 4
    • 0027160056 scopus 로고
    • T. V. P. Bliss and G. L. Collingridge, Nature 361, 31 (1993); Y.-Y. Huang, X. C. Li, E. R. Kandel, Cell 79, 69 (1994); M. Krug, B. Lossner, T. Ott, Br. Res. Bull. 13, 39 (1984); H. Matthies and K. G. Reymann, Neuroreport 4, 712 (1993); P. V. Nguyen, T. Abel, E. R. Kandel, Science 265, 1104 (1994).
    • (1993) Neuroreport , vol.4 , pp. 712
    • Matthies, H.1    Reymann, K.G.2
  • 5
    • 0028038057 scopus 로고
    • T. V. P. Bliss and G. L. Collingridge, Nature 361, 31 (1993); Y.-Y. Huang, X. C. Li, E. R. Kandel, Cell 79, 69 (1994); M. Krug, B. Lossner, T. Ott, Br. Res. Bull. 13, 39 (1984); H. Matthies and K. G. Reymann, Neuroreport 4, 712 (1993); P. V. Nguyen, T. Abel, E. R. Kandel, Science 265, 1104 (1994).
    • (1994) Science , vol.265 , pp. 1104
    • Nguyen, P.V.1    Abel, T.2    Kandel, E.R.3
  • 9
    • 0028800809 scopus 로고
    • M. Ghirardi, P. G. Montarolo, E. R. Kandel, Neuron 14, 413 (1995); E. T. Walters and J. H. Byrne, Science 219, 405 (1983); F. Zhang, S. Endo, L. J. Cleary, A. Eskin, J. H. Byrne, ibid. 275, 1318 (1997).
    • (1995) Neuron , vol.14 , pp. 413
    • Ghirardi, M.1    Montarolo, P.G.2    Kandel, E.R.3
  • 10
    • 0020680046 scopus 로고
    • M. Ghirardi, P. G. Montarolo, E. R. Kandel, Neuron 14, 413 (1995); E. T. Walters and J. H. Byrne, Science 219, 405 (1983); F. Zhang, S. Endo, L. J. Cleary, A. Eskin, J. H. Byrne, ibid. 275, 1318 (1997).
    • (1983) Science , vol.219 , pp. 405
    • Walters, E.T.1    Byrne, J.H.2
  • 11
    • 0031042276 scopus 로고    scopus 로고
    • M. Ghirardi, P. G. Montarolo, E. R. Kandel, Neuron 14, 413 (1995); E. T. Walters and J. H. Byrne, Science 219, 405 (1983); F. Zhang, S. Endo, L. J. Cleary, A. Eskin, J. H. Byrne, ibid. 275, 1318 (1997).
    • (1997) Science , vol.275 , pp. 1318
    • Zhang, F.1    Endo, S.2    Cleary, L.J.3    Eskin, A.4    Byrne, J.H.5
  • 14
    • 0031444409 scopus 로고    scopus 로고
    • K. C. Martin et al., Cell 91, 927 (1997).
    • (1997) Cell , vol.91 , pp. 927
    • Martin, K.C.1
  • 15
    • 0028917678 scopus 로고
    • Although we refer to the two compartments as somatic (pleural ganglion) and synaptic (pedal ganglion) because they include the SN somata and their remote synapses, respectively, both compartments also contain interneurons (INTs) that make synapses with the SNs or MNs, and the SNs make synapses onto proximal INTs in the pleural ganglion [Y. Xu, J. P. Pieroni, L. J. Cleary, J. H. Byrne, J. Neurophysiol. 73, 1313 (1995); L. J. Cleary and J. H. Byrne, ibid. 70, 1767 (1993) ].
    • (1995) J. Neurophysiol. , vol.73 , pp. 1313
    • Xu, Y.1    Pieroni, J.P.2    Cleary, L.J.3    Byrne, J.H.4
  • 16
    • 0027364905 scopus 로고
    • Although we refer to the two compartments as somatic (pleural ganglion) and synaptic (pedal ganglion) because they include the SN somata and their remote synapses, respectively, both compartments also contain interneurons (INTs) that make synapses with the SNs or MNs, and the SNs make synapses onto proximal INTs in the pleural ganglion [Y. Xu, J. P. Pieroni, L. J. Cleary, J. H. Byrne, J. Neurophysiol. 73, 1313 (1995); L. J. Cleary and J. H. Byrne, ibid. 70, 1767 (1993) ].
    • (1993) J. Neurophysiol. , vol.70 , pp. 1767
    • Cleary, L.J.1    Byrne, J.H.2
  • 17
    • 0025108169 scopus 로고
    • We made recordings from monosynaptically connected SN-MN pairs. To prevent firing, we hyperpolarized the MN membrane potential to -70 mV. There was no spontaneous activity in SNs in either artificial seawater or 5HT. We used only synapses that (i) did not decrement (<20% decrease in amplitude) during the three pretests (which were averaged for a baseline) and (ii) showed ST responses during 5HT exposure (>75% of preparations). ST responses included STF, increased SN spike width, and decreased SN spike threshold [D. A. Baxter and J. H. Byrne, J. Neurophysiol. 64, 978 (1990); E. T. Walters, J. H. Byrne, T. J. Carew, E. R. Kandel, ibid. 50, 1543 (1983)]. For procedural details, see (3).
    • (1990) J. Neurophysiol. , vol.64 , pp. 978
    • Baxter, D.A.1    Byrne, J.H.2
  • 18
    • 0021048107 scopus 로고
    • For procedural details, see (3)
    • We made recordings from monosynaptically connected SN-MN pairs. To prevent firing, we hyperpolarized the MN membrane potential to -70 mV. There was no spontaneous activity in SNs in either artificial seawater or 5HT. We used only synapses that (i) did not decrement (<20% decrease in amplitude) during the three pretests (which were averaged for a baseline) and (ii) showed ST responses during 5HT exposure (>75% of preparations). ST responses included STF, increased SN spike width, and decreased SN spike threshold [D. A. Baxter and J. H. Byrne, J. Neurophysiol. 64, 978 (1990); E. T. Walters, J. H. Byrne, T. J. Carew, E. R. Kandel, ibid. 50, 1543 (1983)]. For procedural details, see (3).
    • (1983) J. Neurophysiol. , vol.50 , pp. 1543
    • Walters, E.T.1    Byrne, J.H.2    Carew, T.J.3    Kandel, E.R.4
  • 19
    • 0345105982 scopus 로고    scopus 로고
    • note
    • 3.27 = 0.72; P = 0.55) tests.
  • 21
    • 0345537428 scopus 로고    scopus 로고
    • note
    • 2.12 = 4.99; P = 0.027). Significant STF was expressed in all groups: controls, +154.8 ± 15.5%; synaptic-emetine, +92.9 ± 29.5%; somatic-emetine, +109.7 ± 33.6%; P < 0.02 in all cases.
  • 22
    • 0344243722 scopus 로고    scopus 로고
    • note
    • The magnitude of coincident LTF in the controls as well as in the somatic-emetine (Fig. 3A) and somatic-emetine (4 to 6 hours) (Fig. 3B) groups is quite comparable [see also (3)]. However, the absolute magnitude of LTF in these groups is less than that observed in our initial experiments (Fig.1). We do not know the source of this variability, but it underscores the importance of having each experimental group compared with its own set of controls (run simultaneously), which was the case in all our experiments.
  • 23
    • 0345537427 scopus 로고    scopus 로고
    • note
    • STF: -16.0 ± 2.8%, NS (somatic-emetine); -15.5 ± 2.4%, NS (synaptic-emetine). LTF: -15.8 ± 10.4%, NS (somatic-emetine); -4.0 ± 12.2%, NS (synaptic emetine), paired t tests.
  • 24
    • 0345537426 scopus 로고    scopus 로고
    • note
    • It is possible that our results reflect a capture-like mechanism [see (8)]. For example, if the 25-min 5HT pulse to the somatic compartment induced LTF at SN-INT synapses in the pleural ganglion, the synaptic pulse in the pedal ganglion could be viewed as inducing capture at the remote synapse. If true, this would extend the notion of capture in two ways: (i) in this case, protein synthesis is required immediately at the site of capture, and (ii) there is a tight temporal constraint (15 min) imposed on the induction-capture process occurring at two anatomically remote sites.
  • 26
    • 0344243721 scopus 로고    scopus 로고
    • note
    • 2.11 = 4.24; P = 0.043).
  • 27
    • 0028950156 scopus 로고
    • The synaptic compartment (pedal ganglion) contains both the presynaptic SN terminals and postsynaptic MN neurites and somata. Thus the translation-dependent synaptic signal required for LTF induction (Fig. 3A) could be presynaptic, postsynaptic, or both. Distinguishing between pre- and postsynaptic contributions is experimentally feasible in this system [L.-E. Trudeau and V. F. Castellucci, J. Neurosci. 5, 1275 (1995); D. L. Glanzman, J. Neurobiol. 25, 666 (1994)]. Thus we are currently examining the effects of blocking translation in individual SNs and MNs.
    • (1995) J. Neurosci. , vol.5 , pp. 1275
    • Trudeau, L.-E.1    Castellucci, V.F.2
  • 28
    • 0028235909 scopus 로고
    • The synaptic compartment (pedal ganglion) contains both the presynaptic SN terminals and postsynaptic MN neurites and somata. Thus the translation-dependent synaptic signal required for LTF induction (Fig. 3A) could be presynaptic, postsynaptic, or both. Distinguishing between pre- and postsynaptic contributions is experimentally feasible in this system [L.-E. Trudeau and V. F. Castellucci, J. Neurosci. 5, 1275 (1995); D. L. Glanzman, J. Neurobiol. 25, 666 (1994)]. Thus we are currently examining the effects of blocking translation in individual SNs and MNs.
    • (1994) J. Neurobiol. , vol.25 , pp. 666
    • Glanzman, D.L.1
  • 29
    • 0028860058 scopus 로고
    • The length of the connective is 2 to 3 mm, and the estimated rate of fast transport in Aplysia neurons is about 1.5 mm/hour [J. D. Gunstream, G. A. Castro, E. T. Walters, J. Neurosci. 15, 439 (1995); R. T. Ambron, R. Schmied, C. C. Huang, M. Smedman, ibid. 12, 2813 (1992)]. Thus communication between synapse and soma requires 40 min to >1 hour by this mechanism.
    • (1995) J. Neurosci. , vol.15 , pp. 439
    • Gunstream, J.D.1    Castro, G.A.2    Walters, E.T.3
  • 30
    • 0026688977 scopus 로고
    • The length of the connective is 2 to 3 mm, and the estimated rate of fast transport in Aplysia neurons is about 1.5 mm/hour [J. D. Gunstream, G. A. Castro, E. T. Walters, J. Neurosci. 15, 439 (1995); R. T. Ambron, R. Schmied, C. C. Huang, M. Smedman, ibid. 12, 2813 (1992)]. Thus communication between synapse and soma requires 40 min to >1 hour by this mechanism.
    • (1992) J. Neurosci. , vol.12 , pp. 2813
    • Ambron, R.T.1    Schmied, R.2    Huang, C.C.3    Smedman, M.4
  • 32
    • 0030861966 scopus 로고    scopus 로고
    • D. L. Senger and R. B. Campenot, J. Cell Biol. 138, 411 (1997); R. Stoop and M. Poo, Science 267, 695 (1995); A. Bhattacharyya et al., J. Neurosci. 17, 7007 (1997).
    • (1997) J. Cell Biol. , vol.138 , pp. 411
    • Senger, D.L.1    Campenot, R.B.2
  • 33
    • 0028903917 scopus 로고
    • D. L. Senger and R. B. Campenot, J. Cell Biol. 138, 411 (1997); R. Stoop and M. Poo, Science 267, 695 (1995); A. Bhattacharyya et al., J. Neurosci. 17, 7007 (1997).
    • (1995) Science , vol.267 , pp. 695
    • Stoop, R.1    Poo, M.2
  • 34
    • 0030869198 scopus 로고    scopus 로고
    • D. L. Senger and R. B. Campenot, J. Cell Biol. 138, 411 (1997); R. Stoop and M. Poo, Science 267, 695 (1995); A. Bhattacharyya et al., J. Neurosci. 17, 7007 (1997).
    • (1997) J. Neurosci. , vol.17 , pp. 7007
    • Bhattacharyya, A.1
  • 35
    • 0024552205 scopus 로고
    • In CA1 cells of the hippocampus, the soma is not essential for induction of long-term synaptic plasticity (12) [U. Frey, M. Krug, R. Brodemann, K. Reymann, H. Matthies, Neurosci. Lett. 97, 135 (1989)]; however, this does not indicate that the soma is unable to participate in the induction process, especially under conditions of subthreshold activation at remote synaptic sites.
    • (1989) Neurosci. Lett. , vol.97 , pp. 135
    • Frey, U.1    Krug, M.2    Brodemann, R.3    Reymann, K.4    Matthies, H.5
  • 36
    • 0345105979 scopus 로고    scopus 로고
    • note
    • Supported by National Institutes of Health grant F32-MH12004 to C.M.S. and National Institute of Mental Health grant RO1MH-14-1083 to T.J.C. We thank E. Kandel, S. McKay, and M. Sutton for helpful comments on a previous draft of this paper.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.