Role of transforming growth factor-β in long-term synaptic facilitation in Aplysia

Science

Volumn 275, Issue 5304, 1997, Pages 1318-1320

  Zhang, Fan ^a   Endo, Shogo ^b   Cleary, Leonard J ^a   Eskin, Arnold ^b   Byrne, John H ^a  

^a UNIVERSITY OF TEXAS   (United States)

^b UNIVERSITY OF HOUSTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

TRANSFORMING GROWTH FACTOR BETA;

ANIMAL CELL; APLYSIA; ARTICLE; CONTROLLED STUDY; EXCITATORY POSTSYNAPTIC POTENTIAL; LEARNING; MEMORY; MOTONEURON; NERVE CELL PLASTICITY; NONHUMAN; PRIORITY JOURNAL; SENSORY NERVE CELL; SYNAPSE; WITHDRAWAL REFLEX;

ANIMALS; APLYSIA; ELECTRIC STIMULATION; MOTOR NEURONS; NEURONAL PLASTICITY; NEURONS, AFFERENT; RECEPTORS, TRANSFORMING GROWTH FACTOR BETA; SEROTONIN; SIGNAL TRANSDUCTION; SYNAPSES; SYNAPTIC TRANSMISSION; TRANSFORMING GROWTH FACTOR BETA;

ANIMALIA; APLYSIA;

EID: 0031042276     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.275.5304.1318     Document Type: Article

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28. A TGF-β-like molecule was found in Aplysia hemolymph with an antibody to human TGF-β1 (S. Endo et al., unpublished data).
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31. Human TGF-β (TGF-β1, R&D Systems) was used in the experiments. Lyophilized recombinant human TGF-β1 samples were reconstituted with BSA solution. The final concentration of BSA was adjusted to 10 μg/ml in all the experiments. In control experiments, only BSA solution was used. In pilot experiments, no effects were observed when TGF-β1 was used at 10 ng/ml. The relatively high dose of TGF-β1 required to induce long-term facilitation could be attributable to a difference in potency of the species-specific forms. However, the concentration of TGF-β1 in human serum is >30 ng/ml [I. E. Eder et al., J. Urol. 156, 953 (1996)]. There are currently no known data from Aplysia or other systems regarding the effective duration of TGF-β application. Therefore, TGF-β1 was applied for 24 hours to provide sufficient time to induce long-term facilitation.
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56. Supported by Air Force Office of Scientific Research grant F49620-92-J-0494 (A.E.) and NIH grants RO1-NS-28462 (A.E.), RO1-NS-19895 (J.H.B.), and K05 MH-00649 (J.H.B.).