Surface crystallisation of the plasma membrane H+-ATPase on a carbon support film for electron crystallography

Journal of Molecular Biology

Volumn 287, Issue 5, 1999, Pages 961-968

  Auer, Manfred ^a,c   Scarborough, Gene A ^b   Kühlbrandt, Werner ^a  

^a MAX PLANCK INSTITUTE OF BIOPHYSICS   (Germany)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

Author keywords

2D crystal; Electron crystallography; Membrane protein; P type H+ ATPase; Surface crystallisation

Indexed keywords

ADENOSINE TRIPHOSPHATASE; CELL MEMBRANE PROTEIN; DETERGENT; MEMBRANE ENZYME;

ARTICLE; CONTROLLED STUDY; CRYSTALLOGRAPHY; ELECTRON CRYSTALLOGRAPHY; ELECTRON MICROSCOPY; FOURIER TRANSFORMATION; HYDROPHILICITY; HYDROPHOBICITY; PRIORITY JOURNAL;

EID: 0033574521     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.2652     Document Type: Article

References (40)

1. Aoyoma K., Ogawa K., Kimura Y., Fujiyoshi Y. A method for 2D crystallisation of soluble proteins at liquid-liquid interface. Ultramicroscopy. 57:1995;345-354.
2. +-ATPase in the open conformation. Nature. 392:1998;840-843.
3. Boekema E. J., Böttcher B. The structure of ATP synthase from chloroplasts. Conformational changes of CF1 studied by electron microscopy. Biochim. Biophys. Acta. 1098:1992;131-143.
4. +-ATPase. Arch. Biochim. Biophys. 252:1987;348-356.
5. Cheng A., v. Hoek A. N., Yeager M., Verkman A. S., Mitra A. Three-dimensional organisation of a human water channel. Nature. 387:1997;627-630.
6. Acta Crystallog. sect. D. 50:1994;760-763.
7. Cowan S. W., Schirmer T., Rummel G., Steiert M., Ghosh R., Pauptit R. A., Jansonius J. N., Rosenbusch J. P. Crystal structures explain functional properties of two E. coli porins. Nature. 358:1992;727-733.
8. Crowther R. A., Henderson R., Smith J. M. MRC image processing programs. J. Struct. Biol. 116:1996;9-16.
9. Cyrklaff M., Auer M., Kühlbrandt W., Scarborough G. A. 2-D structure of the Neurospora crassa plasma membrane ATPase as determined by electron cryomicroscopy. EMBO J. 14:1995;1854-1857.
10. Deisenhofer J., Epp O., Miki K., Huber R., Michel H. X-ray structure analysis of a membrane-protein complex: electron-density map at 3 Å resolution and a model of the chromophores of the photosynthetic reaction centre from Rhodopseudomonas viridis. J. Mol. Biol. 180:1984;385-398.
11. + conduction and selectivity. Science. 280:1998;69-77.
12. Dubochet J., Adrian M., Chang J.-J., Homo J.-C., Lepault J., McDowall A. W., Schultz P. Cryo-electron microscopy of vitrified specimens. Quart. Rev. Biophys. 21:1988;129-228.
13. Hegerl R. The EM package: a platform for image processing in biological electron microscopy. J. Struct. Biol. 116:1996;30-34.
14. Henderson R. The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules. Quart. Rev. Biophys. 28:1995;171-193.
15. Henderson R., Baldwin J. M., Downing K. H., Lepault J., Zemlin F. Structure of purple membrane from Halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolution. Ultramicroscopy. 19:1986;147-178.
16. Henderson R., Baldwin J. M., Ceska T. A., Zemlin F., Beckmann E., Downing K. H. Model for the structure of bacteriorhodopsin based on high-resolution electron cryomicroscopy. J. Mol. Biol. 213:1990;899-929.
17. Iwata S., Ostermeier C., Ludwig B., Michel H. Structure at 2.8 Å resolution of cytochrome c oxidase from Paracoccus denitrificans. Nature. 376:1995;660-669.
18. Jap B., Zulauf M., Scheybani T., Hefti A., Baumeister W., Aebi U., Engel A. 2D crystallisation: from art to science. Ultramicroscopy. 46:1992;45-84.
19. Koepke J., Hu X., Muenke C., Schulten K., Michel H. The crystal structure of the light-harvesting complex II (B800-850) from Rhodospirillum molischianum. Structure. 4:1996;581-597.
20. Kornberg R. D., Darst S. A. 2D crystals of proteins on lipid layers. Curr. Opin. Struct. Biol. 1:1991;642-646.
21. Kühlbrandt W. Two-dimensional crystallisation of membrane proteins. Quart. Rev. Biophys. 21:1992;429-477.
22. Kühlbrandt W., Wang D. N., Fujiyoshi Y. Atomic model of plant light harvesting complex by electron crystallography. Nature. 367:1994;614-621.
23. Li H., Lee S., Jap B. Molecular design of aquaporin-1 water channel as revealed by electron crystallography. Nature Struct. Biol. 4:1997;263-265.
24. McDermott G., Prince S. M., Freer A. A., Hawthornthwaite-Lawless A. M., Papitz M. Z., Cogdell R. J., Isaacs N. W. Crystal structure of an integral membrane light-harvesting complex from photosynthetic bacteria. Nature. 374:1995;517-521.
25. Michel H. Three-dimensional crystals of a membrane protein complex. The photosynthetic reaction centre from Rhodopseudomonas viridis. J. Mol. Biol. 158:1982;567-572.
26. Pebay-Peroula E., Rummel G., Rosenbusch J. P., Landau E. M. High resolution X-ray structure of bacteriorhodopsin from microcrystals grown in lipidic cubic phases. Science. 277:1997;1676-1680.
27. Rigaud J.-L., Mosser G., Lacapere J.-L., Olofsson A., Levi D., Ranck J.-L. Bio-Beads: an efficient strategy for two-dimensional crystallisation of membrane proteins. J. Struct. Biol. 118:1997;226-235.
28. +-ATPase: an approach to the crystallisation of integral membrane proteins. Acta Crystallog. sect. D. 50:1994;643-649.
29. Schertler G. F. X. Orthorhomic crystal form of bacteriorhodopsin nucleated on benzamidine diffracting to 3.6 Å resolution. J. Mol. Biol. 234:1993;156-164.
30. Song L., Hobaugh M. R., Shustak C., Cheley S., Bayley H., Gouaux J. E. Structure of staphylococcal α-hemolysin, a heptameric transmembrane pore. Science. 274:1996;1859-1866.
31. Tsukihara T., Aoyama H., Yamashita E., Tomizaki T., Yamaguchi H., Shinzawa-Itoh K., Nakashima R., Yaona R., Yoshikawa S. Structure of metal sites of oxidised bovine heart cytochrome c oxidase at 2.8 Å Science. 269:1995;1069-1074.
32. Unger V. M., Hargave P. A., Baldwin J. M., Schertler G. F. X. Arrangement of rhodopsin transmembrane alpha-helices. Nature. 389:1997;203-206.
33. Unwin P. N. T. Acetylcholine receptor channel imaged in the open state. Nature. 373:1995;37-43.
34. Vonck J., van Bruggen E. F. J. Electron microscopy and image analysis of two-dimensional crystals and single molecules of alcohol oxidase from Hansenula polymorpha. Biochim. Biophys. Acta. 1038:1990;74-79.
35. Walz T., Hirai T., Murata K., Heymann J. B., Mitsuoka K., Fujiyoshi Y., Smith B. L., Agre P., Engel A. The three-dimensional structure of aquaporin-1. Nature. 387:1997;624-627.
36. Weiss M. S., Abele U., Weckesser J., Welte W., Schiltz E., Schulz G. E. Molecular architecture and electrostatic properties of a bacterial porin. Science. 254:1991;1627-1630.
37. Yoshimura H., Matsumoto M., Endo S., Nagayama K. Two-dimensional crystallisation of proteins on mercury. Ultramicroscopy. 32:1990;265-274.
38. 1 complex. Science. 277:1997;1136-1144.
39. Zhang P., Toyoshima C., Yonekura K., Green N. M., Stokes D. L. Structure of the calcium pump from sarcoplasmic reticulum at 8 Å resolution. Nature. 392:1998a;835-839.
40. 1. Nature. 392:1998b;677-684.