Activation of the rod G-protein G(t) by the thrombin receptor (PAR1) expressed in Sf9 cells

European Journal of Biochemistry

Volumn 266, Issue 3, 1999, Pages 911-916

  Seibert, Christoph ^a   Harteneck, Christian ^b   Ernst, Oliver P ^a   Schultz, Günter ^b   Hofmann, Klaus P ^a,c  

^a HUMBOLDT UNIVERSITY   (Germany)

^b FREIE UNIVERSITÄT BERLIN   (Germany)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

G(t); G protein coupled receptor; PAR1; Sf9 cells; Thrombin receptor

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' O (3 THIOTRIPHOSPHATE); RHODOPSIN; SERINE PROTEINASE; THROMBIN RECEPTOR; TRANSDUCIN;

ARTICLE; BACULOVIRUS; CATTLE; GENE EXPRESSION; INSECT; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; RETINA ROD OUTER SEGMENT; SIGNAL TRANSDUCTION; THROMBOSIS;

ANIMALS; CATTLE; CELL LINE; GENE EXPRESSION; GUANINE NUCLEOTIDES; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; RECEPTOR, PAR-1; RECEPTORS, THROMBIN; RHODOPSIN; SPODOPTERA; TRANSDUCIN;

EID: 0033572969     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00927.x     Document Type: Article

References (42)

1. 1. Shuman, M.A. (1986) Thrombin-cellular interactions. Ann. N.Y. Acad. Sci. 485, 228-239.
2. 2. Fenton, J.W. II (1988) Regulation of thrombin generation and functions. Semin. Thromb. Hemost. 14, 234-240.
3. 3. Coughlin, S.R., Vu, T.-K.H., Hung, D.T. & Wheaton, V.I. (1992) Characterization of a functional thrombin receptor. Issues and opportunities. J. Clin. Invest. 89, 351-355.
4. 4. Vu, T.-K.H., Hung, D.T., Wheaton, V.I. & Coughlin, S.R. (1991) Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell 64, 1057-1068.
5. 5. Coughlin, S.R. (1994) Protease-activated receptors start a family. Proc. Natl Acad. Sci. USA 91, 9200-9202.
6. 6. Kahn, M.L., Hammes, S.R., Botka, C. & Coughlin, S.R. (1998) Gene and locus structure and chromosomal localization of the protease-activated receptor gene family. J. Biol. Chem. 273, 23290-23296.
7. 7. Vu, T.-K.H., Wheaton, V.I., Hung, D.T., Charo, I. & Coughlin, S.R. (1991) Domains specifying thrombin-receptor interaction. Nature (London) 353, 674-677.
8. 8. Chen, J., Ishii, M., Wang, L., Ishii, K. & Coughlin, S.R. (1994) Thrombin receptor activation. Confirmation of the intramolecular tethered liganding hypothesis and discovery of an alternative intermolecular liganding mode. J. Biol. Chem. 269, 16041-16045.
9. 9. Hung, D.T., Wong, Y.H., Vu, T.K.H. & Coughlin, S.R. (1992) The cloned platelet thrombin receptor couples to at least two distinct effectors to stimulate phosphoinositide hydrolysis and inhibit adenylyl cyclase. J. Biol. Chem. 267, 20831-20834.
10. 10. Offermanns, S., Laugwitz, K.-L., Spicher, K. & Schultz, G. (1994) G proteins of the G12 family are activated via thromboxane A2 and thrombin receptors in human platelets. Proc. Natl Acad. Sci. USA 91, 504-508.
11. 11. Gudermann, T., Kalkbrenner, F. & Schultz, G. (1996) Diversity and selectivity of receptor-G-protein interaction. Anna. Rev. Pharmacol. Toxicol. 36, 429-459.
12. 12. Helmreich, E.J. & Hofmann, K.P. (1996) Structure and function of proteins in G-protein-coupled signal transfer. Biochim. Biophys. Acta 1286, 285-322.
13. 13. Oprian, D.D. (1992) The ligand-binding domain of rhodopsin and other G protein-linked receptors. J. Bioenerg. Biomem. 24, 211-217.
14. 14. Jäger, S., Palczewski, K. & Hofmann, K.P. (1996) Opsin/all-transretinal complex activates transducin by different mechanisms than photolyzed rhodopsin. Biochemistry 35, 2901-2908.
15. 15. Buczylko, J., Saari, J.C., Crouch, R.K. & Palczewski, K. (1996) Mechanisms of opsin activation. J Biol. Chem. 271, 20621-20630.
16. 16. Han, M., Smith, S.O. & Sakmar, T.P. (1998) Constitutive activation of opsin by mutation of methionine 257 on transmembrane helix 6. Biochemistry 37, 8253-8261.
17. 17. Cohen, G.B., Oprian, D.D. & Robinson, P.R. (1992) Mechanism of activation and inactivation of opsin: role of Glu113 and Lys296. Biochemistry 31, 12592-12601.
18. 18. Fahmy, K. & Sakmar, T.P. (1993) Regulation of the rhodopsin-transducin interaction by a highly conserved carboxylic acid group. Biochemistry 32, 7229-7236.
19. 19. Cascio, M. (I995) Baculovirus expression of receptors and channels. Methods Neurosci. 25, 175-200.
20. 20. De Caluwé, L.L.J., Van Oostrum, J., Janssen, J.J.M. & De Grip, W.J. (1993) In vitro synthesis of bovine rhodopsin using recombinant baculovirus. Methods Neurosci. 15, 307-321.
21. 21. Harteneck, C., Obukhov, A.G., Zobel, A., Kalkbrenner, F. & Schultz, G. (1995) The Drosophila cation channel trpl expressed in insect Sf9 cells is stimulated by agonists of G-protein-coupled receptors. FEBS Lett. 358, 297-300.
22. 22. Chen, X., Earley, K., Luo, W., Lin, S.-H. & Schilling, W.P. (1996) Functional expression of a human thrombin receptor in Sf9 insect cells: evidence for an active tethered ligand. Biochem, J. 314, 603-611.
23. 23. Barr, A.J., Brass, L.F. & Manning, D.R. (1997) Reconstitution of receptors and GTP-binding regulatory proteins (G proteins) in Sf9 cells. A direct evaluation of selectivity in receptor. G protein coupling. J. Biol. Chem. 272, 2223-2229.
24. 24. Sachs, K., Maretzki, D. & Hofmann, K.P. (1999) Assays for the activation of opsin by all-trans-retinal. Methods Enzymol. 315, 238-251.
25. 25. Merrifield, R.B. (1963) Solid phase peptide synthesis. I. The synthesis of a tetrapeptide. J. Am. Chem. Soc. 85, 2149-2154.
26. 26. Carpino, L.A. & Han, G.Y. (1970) The 9-fluorenylmethoxycarbonyl function, a new base-sensitive amino-protection group. J. Am. Chem. Soc. 92, 5748-5749.
27. 27. Carpino, L.A. & Han, G.Y. (1972) The 9-fluorenylmethoxycarbonyl amino-protecting group. J. Org. Chem. 37, 3404-3409.
28. 28. Heck, M. & Hofmann, K.P. (1993) G-protein-effector coupling: a real-time light-scattering assay for transducin-phosphodiesterase interaction. Biochemistry 32, 8220-8227.
29. 29. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
30. 30. O'Reilly, D.R., Miller, L.K. & Luckow, V.A. (1992). Baculovirus Expression Vectors: a Laboratory Manual. W. H. Freeman, New York, USA.
31. 31. Higashijima, T., Ferguson, K.M., Sternweis, P.C., Ross, E.M., Smigel, M.D. & Gilman, A.G. (1987) The effect of activating ligands on the intrinsic fluorescence of guanine nucleotide-binding regulatory proteins. J. Biol. Chem. 262, 752-756.
32. O. J. Biol. Chem. 262, 757-761.
33. 33. Phillips, W.J. & Cerione, R.A. (1988) The intrinsic fluorescence of the alpha subunit of transducin. Measurement of receptor-dependent guanine nucleotide exchange. J. Biol. Chem. 263, 15498-15505.
34. 34. Faurobert, E., Otto-Bruc, A., Chardin. P. & Chabre, M. (1993) Tryptophan W207 in transducin T alpha is the fluorescence sensor of the G protein activation switch and is involved in the effector binding. EMBO J. 12, 4191-4198.
35. 35. Simon, M.I., Strathmann, M.P. & Gautam, N. (1991) Diversity of G proteins in signal transduction. Science 252, 802-808.
36. 36. Kahlert, M. & Hofmann, K.P. (1991) Reaction rate and collisional efficiency of the rhodopsin-transducin system in intact retinal rods. Biophys. J. 59, 375-386.
37. 37. Clawges, H.M., Depree, K.M., Parker, E.M. & Graber, S.G. (1997) Human 5-HT1 receptor subtypes exhibit distinct G protein coupling behaviors in membranes from Sf9 cells. Biochemistry 36, 12930-12938.
38. 38. Kahlert, M., König, B. & Hofmann, K.P. (1990) Displacement of rhodopsin by GDP from three-loop interaction with transducin depends critically on the diphosphate beta-position. J. Biol. Chem. 265, 18928-18932.
39. 39. Bourne, H.R., Masters, S.B., Miller, R.T., Sullivan, K.A. & Heideman, W. (1988) Mutations probe structure and function of G-protein alpha chains. Cold Spring Harb. Symp Quant. Biol. 53, 221-228.
40. 40. Arnis, S., Fahmy, K., Hofmann, K.P. & Sakmar, T.P. (1994) A conserved carboxylic acid group mediates light-dependent proton uptake and signaling by rhodopsin. J. Biol. Chem. 269, 23879-23881.
41. 41. Scheer, A., Fanelli, F., Costa, T., De Benedetti, P.G. & Cotecchia, S. (1996) Constitutively active mutants of the alpha IB-adrenergic receptor: role of highly conserved polar amino acids in receptor activation. EMBO J. 15, 3566-3578.
42. 42. Kim, J.M., Altenbach, C., Thurmond, R.L., Khorana, H.G. & Hubbell, W.L. (1997) Structure and function in rhodopsin: rhodopsin mutants with a neutral amino acid at E134 have a partially activated conformation in the dark state. Proc. Natl Acad. Sci. USA 94, 14273-14278.