Production and activation of recombinant hK2 with propeptide mutations resulting in high expression levels

European Journal of Biochemistry

Volumn 266, Issue 3, 1999, Pages 1050-1055

  Lövgren, Janita ^a,b   Tian, Sha ^c   Lundwall, Åke ^a   Karp, Matti ^d   Lilja, Hans ^a  

^a LUND UNIVERSITY   (Sweden)

^b UNIVERSITY OF TURKU   (Finland)

^c UNIVERSITY OF HELSINKI   (Finland)

^d NONE

Author keywords

Cancer; Expression; Kallikrein; Prostate; Zymogen

Indexed keywords

COMPLEMENTARY DNA; ENZYME PRECURSOR; KALLIKREIN; PROSTATE SPECIFIC ANTIGEN; TUMOR MARKER;

ANTIGEN EXPRESSION; ARTICLE; HUMAN; KALLIKREIN KININ SYSTEM; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROSTATE CANCER; PROSTATE HYPERTROPHY; PROTEIN PURIFICATION;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE; DNA PRIMERS; ENZYME ACTIVATION; ENZYME PRECURSORS; GENE EXPRESSION; HUMANS; KINETICS; MALE; MUTATION; PROSTATE; RECOMBINANT PROTEINS; SPODOPTERA; TISSUE KALLIKREINS;

EID: 0033572917     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00946.x     Document Type: Article

References (28)

1. 1. Clements, J.A. (1989) The glandular kallikrein family of enzymes: Tissue-specific expression and hormonal regulation. Endocrinol. Rev. 10, 393-419.
2. 2. Schedlich, L.J., Bennetts, B.H. & Morris, B.J. (1987) Primary structure of a human glandular kallikrein gene. DNA 6, 429-437.
3. 3. Drinkwater, C.C., Evans, B.A. & Richards, R.I. (1988) Kallikreins, kinins and growth factor biosynthesis. Trends Biochem. Sci. 13, 169-172.
4. 4. Lövgren, J., Valtonen-André, C., Marsal, K., Lilja, H. & Lundwall, Å. (1999) Measurement of prostate-specific antigen and human glandular kallikrein 2 in different body fluids. J. Androl. 20, 348-355.
5. 5. Kwiatkowski, M., Recker, F., Piironen, T., Pettersson, K., Otto, T., Wernli, M. & Tscholl, R. (1998) In prostatism patients the ratio of human glandular kallikrein to free PSA improves the discrimination between prostate cancer and benign hyperplasia within the diagnostic 'gray zone' of total PSA 4-10 ng/ml. Urology 52, 360-365.
6. 6. Recker, F., Kwiatkowski, M.K., Piironen, T., Pettersson, K., Lümmen, G., Wernil, M., Wlefelspütz, J., Goepel, M., Huber, A. & Tscholl, R. (1998) The importance of human glandular kallikrein and its correlation with different prostate specific antigen serum forms in the detection of prostate carcinoma. Cancer 83, 2540-2547.
7. 7. Clements, J.A., Mukthar, A., Verity, K., Pullar, M., McNeill, P., Cummins, J. & Fuller, P.J. (1996) Kallikrein gene expression in human pituitary tissue. Clin. Endcrinol. 44, 223-231.
8. 8. Hsieh, M.-L., Charlesworth, C., Goodmanson, M., Zhang, S., Seay, T., Klee, G.G., Tindall, D.J. & Young, C.Y.F. (1997) Expression of human prostate-specific glandular kallikrein protein (hK2) in the breast camcer cell line T47-D. Cancer Res. 57, 897-899.
9. 9. Lövgren, J., Airas, K. & Lilja, H. (1999) Enzymatic action of human glandular kallikrein 2 (hK2): substrate specificity and regulation by zinc ions and extracellular protease inhibitors. Eur. J. Biochem. 262, 781-789.
10. 10. Lövgren, J., Rajakoski, K., Karp, M., Lundwall, Å. & Lilja, H. (1997) Activation of the zymogen form of prostate-specific antigen by human glandular kallikrein 2. Biochem. Bioph. Res. Commun. 238, 549-555.
11. 11. Takayama, T.K., Fujikawa, K. & Davie, E.W. (1997) Characterization of the precursor of prostate-specific antigen. J. Biol. Chem. 272, 21582-21588.
12. 12. Kumar, A., Mikolajczyk, S.D., Goel, A.S., Millar, L.S. & Saedi, M.S. (1997) Expression of pro form of prostate-specific antigen by mammalian cells and its conversion to mature, active form by human kallikrein 2. Cancer Res. 57, 3111-3114.
13. 13 Mikolajczyk, S.D., Millar, L.S., Marker, K.M., Grauer, L.S., Goel, A., Cass, M.M.J., Kumar, A. & Saedi, M.S. (1997) Ala217 is important for the catalytic function and autoactivation of prostate-specific human kallikrein 2. Eur. J. Biochem. 246, 440-446.
14. 14. Deperthes, D., Frenette, G., Brilliard-Bourdet, Bourgeous, L., Gauthier, F., Tremblay, R.R. & Dubé, J.Y. (1996) Potential invplvement of kallikrein hK2 in the hydrolysis of the human seminal vesicle proteins after ejaculation. J. Androl. 17, 659-665.
15. 15. Pattison, S.E. & Dunn, M.F. (1975) On the relationship of zinc ion to the structure and function of the 7S nerve growth factor protein. Biochemistry 14, 2733-2739.
16. 16. Deperthes, D., Chapdelaine, P., Tremblay, R.R., Brunet, C., Berton, J., Hébert, J., Lazure, C., Duhé, J. & Y. (1995) Isolation of prostatic kallikrein hK2, also known as hGK-1, in human seminal plasma. Biochim. Biophys. Acta 1245, 311-316.
17. 17. Lövgren, J., Piironen, T., Övermo, C., Dowell, B., Karp, M., Pettersson, K., Lilja, H. & Lundwall, Å. (1995) Production of recombinant PSA and hK2 and analysis of their immunologic cross-reactivity. Biochem. Bioph. Res. Commun 213, 888-895.
18. 18. Rajakoski, K., Piironen, T., Pettersson, K., Lövgren, J. & Karp. M. (1997) Epitope mapping of human prostate-specific antigen and glandular kallikrein expressed in insect cells. Prostate Cancer Prostatic Dis. 1, 16-20.
19. 19. Kumar, A., Goel, A.S., Hill, T.M., Mikolajczyk, S.D., Millar, L.S., Kuus-Reichel, K. & Saedi, M.S. (1996) Expression of human glandular kallikrein, hK2, in mammalian cells. Cancer Res. 56, 5397-5402.
20. 20. Summers, M.D. & Smith, G.E. (1987) A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures. Texas Agricultural Experiment Station Bulletin no. 1555. Texas A & M University, College Station, TX, USA.
21. 21. Eerola, R., Piironen, T., Pettersson, K., Lövgren, J., Vehniäinen, M., Lilja, H., Dowell, B., Lövgren, T. & Karp, M. (1997) Immunoreactivity of recombinant human glandular kallikrein using monoclonal antibodies raised against prostate-specific antigen. The Prostate. 31, pp. 84-90.
22. 22. Piironen, T., Villoutreix, B.O., Becker, C., Hollingsworth, K., Vihinen, M., Bridon, D., Qiu, X., Rapp, J., Dowell, B., Lövgren, T., Pettersson, K. & Lilja, H. (1998) Determination and analysis of antigenic epitopes of prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2) using synthetic peptides and computer modeling. Protein Sci. 7, 259-269.
23. 23. Kumar, A., Goel, A.S., Payne, J.K., Evans, C., Mikolajczyk, S.D., Kuus-Reichel, K. & Saedi, M.S. (1999) Large-scale propagation of recombinant adherent cells that secrete a stable form of human glandular kallikrein, hK2. Protein Exp. Purif. 15, 62-68.
24. 24. Christensson, A., Laurell, C.-B. & Lilja, H. (1990) Enzymatic activity of prostate-specific antigen and it's reactions with extracellular serine proteinase inhibitors, Eur. J. Biochem. 194, 755-763.
25. 25. Khan, A.R. & James, N.G. (1998) Molecular mechanisms for the conversion of zymogens to active proteolytic enzymes. Protein Sci. 7, 815-836.
26. 26. Chapdeline, P., Paradis, G., Tremblay, R.R. & Dubé, J.Y. (1988) High level of expression in the prostate of a human glandular kallikrein mRNA related to prostate-specific antigen. FEBS Lett. 236, 205-208.
27. 27. Henttu, P., Lukkarinen, O. & Vihko, P. (1990) Expression of the gene coding for human prostate-specific antigen and related hGK-1 in benign and malignant tumors of the human prostate. Int. J. Cancer. 45, 654-660.
28. 28. Young, C.Y.-F., Andrews, P.E., Montgomery, B.T. & ans Tindall, D.J. (1992) Tissue-specific and hormonal regulation of human prostate-specific glandular kallikrein. Biochemistry 31, 818-824.