Expression of pro form of prostate-specific antigen by mammalian cells and its conversion to mature, active form by human kallikrein 2

Cancer Research

Volumn 57, Issue 15, 1997, Pages 3111-3114

  Kumar, Abhay ^a   Mikolajczyk, Stephen D ^a   Goel, Amita S ^a   Millar, Lisa S ^a   Saedi, Mohammad S ^a  

^a Hybritech Incorporated   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1 ANTICHYMOTRYPSIN; KALLIKREIN; KALLIKREIN 2; PROSTATE SPECIFIC ANTIGEN; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANTIGEN EXPRESSION; ANTIGEN PRESENTATION; ARTICLE; NONHUMAN; PRIORITY JOURNAL; PROSTATE EPITHELIUM; SYRIAN HAMSTER;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELLS, CULTURED; CHROMATOGRAPHY; CRICETINAE; CULTURE MEDIA, CONDITIONED; HUMANS; KALLIKREINS; MOLECULAR SEQUENCE DATA; PROSTATE-SPECIFIC ANTIGEN; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT PROTEINS; TRANSFECTION;

EID: 0030877244     PISSN: 00085472     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Catalona, W. J., Smith, D. S., Ratliff, T. L., Dodds, K. M., Coplen, D. E., Yuan, I. i., Tetros, J. A., and Andriole, G. L. Measurement of prostate specific antigen in serum as a screening test for prostate cancer. N. Engl. J. Med., 324: 1156-1161, 1991.
2. McCormack, R. T., Rittenhouse, H. G., Finlay, J. A., Sokoloff. R. L., Wang, T. J., Wolfen, R. L., Lilja, H., and Oesterling, J. E. Molecular forms of prostate-specific antigen and the human kallikrein gene family: a new era. Urology, 45: 729-744, 1995.
3. Malm, J., and Lilja, H. Biochemistry of prostate specific antigen, PSA. Scand. J. Clin. Lab. Invest., 221 (Suppl. 5): 15-22, 1995.
4. Young, C. Y. F., Andrews, P. E., and Tindall, D. J. Expression and androgenic regulation of human prostate-specific kallikreins. J. Androl., 16: 97-99, 1995.
5. Lundwall, A., and Lilja, H. Molecular cloning of human prostate-specific antigen cDNA. FEBS Lett., 214: 317-322, 1987.
6. Qui, S., Young, C. Y. F., Bilhartz, D. L., Prescott, J. L., Farrow, G. M., He, W. W., and Tindall, D. J. In situ hybridization of prostate-specific antigen mRNA in human prostate. J. Urol., 144: 1550-1556, 1990.
7. Young, C. Y. F., Montgomery, B. T., Andrews, P. E., Qui, S., Bilhartz, D. L., and Tindall, D. J. Hormonal regulation of prostate-specific antigen mRNA in a human prostatic adenocarcinoma cell line LNCaP. Cancer Res., 51: 3748-3752, 1991.
8. Watt, K. W., Lee, P-J., M'TimKulu, T., Chan, W-P., and Loor, R. Human prostatespecific antigen: structural and functional similarity with serine proteases. Proc. Nail. Acad. Sei. USA, 83: 3166-3170, 1986.
9. Ban, Y., Wang, M. C., Watt, K. W. K., Loor, R., and Chu, T. M. The proteolytic activity of human prostate-specific antigen. Biochem. Biophys. Res. Commun., 123: 482-488, 1984.
10. Lilja, H. A kallikrein-like serine protease in prostatic fluid cleaves the predominant seminal vesicle protein. J. Clin. Invest., 76: 1899-1903, 1985.
11. Cohen, P., Graves, H. C. B., Peehl, D. M., Kamarei, M., Giudice, L. C., and Rosenfeld, R. G. Prostate-specific antigen is an insulin-like growth factor binding protein-3 protease found in seminal plasma. J. Clin. Endocrinol. & Metab., 75: 1046-1053, 1992.
12. Fukushima, D., Kitamura, N., and Nakanishi, S. Nucleotide sequence of cloned cDNA for human pancreatic kallikrein. Biochemistry, 24: 8037-8043, 1985.
13. Morris, B. J. hGK-1: a kallikrein gene expressed in human prostate. Clin. Exp. Pharm. Phys., 16: 345-351, 1989.
14. Young, C. Y. F., Seay, T., Hogen, K., Charlesworth, M. C, Roche, P. C., Klee, G. G., and Tindall, D. J. Prostate-specific human glandular kallikrein (hK2) as a novel marker for prostate cancer. Prostate Suppl., 7: 17-24, 1996.
15. Mikolajczyk, S. D., Millar, L. S., Kumar, A., and Saedi, M. S. Human glandular kallikrein (hK2) shows arginine-restricted specificity and forms complexes with plasma protease inhibitors. Prostate, 33: in press, 1997.
16. Deperthes, D., Frcnette, G., Brillard-Bourdet, M., Bourgois, L., Gauthier, F., Tremblay, R., and Dube, J. Y. Potential involvement of kallikrein hK2 in the hydrolysis of the human seminal vesicle proteins after ejaculation. J. Androl., 17: 659-665, 1996.
17. Kumar, A., Goel, A. S., Hill, T. M., Mikolajczyk, S. D., Millar, L. S., Kuus-Reichel, K., and Saedi, M. S. Expression of human glandular kallikrein, hK2, in mammalian cells. Cancer Res., 56: 5397-5402, 1996.
18. Wang, T. J., Finaly, J. F., Mikolajczyk, S., Rittenhouse, H. G., and Wolfert, R. L. Analysis of cross-reactivity of anti-PSA monoclonal antibodies with recombinant human glandular kallikrein. J. Urol., 155: 695A, 1996.
19. Christensson, A., Laurell, C-B., and Lilja, H. Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors. Eur. J. Bio-chem., 194: 755-763, 1990.
20. Wang, T. J., Linton, H. J., Sokoloff, R. L., Grauer, L. S., Rittenhouse, H. G., and Wolfert, R. L. Antibody specificities for PSA and PSA fragments by SDS-PAGE Western blot analysis. Tumor Biol., in press, 1997.
21. Mikolajczyk, S. D., Millar, L. S., Marker, K. M., Grauer, L. S., Goel, A. S., Cass, M. M. J., Kumar, A., and Saedi, M. S. Ala 217 is important for the catalytic function and autoactivation of prostate-specific human kallikrein 2, hK2. Eur. J. Biochem., 246: 440-446, 1997.