Potential involvement of kallikrein hK2 in the hydrolysis of the human seminal vesicle proteins after ejaculation

Journal of Andrology

Volumn 17, Issue 6, 1996, Pages 659-665

  Deperimes, David ^a   Frenette, Gilles ^a   Brillard Bourdet, Michèle ^b   Bourgeois, Luc ^b   Gauthier, Françis ^b   Tremblay, Roland R ^a   Dubé, Jean Y ^a  

^a LAVAL UNIVERSITY   (Canada)

^b UNIVERSITÉ DE TOURS   (France)

Author keywords

fibronectin; prostate specific antigen; protein C inhibitor; semenogelin; Seminal plasma

Indexed keywords

FIBRONECTIN; KALLIKREIN; PROSTATE SPECIFIC ANTIGEN; PROTEIN; SEMENOGELIN; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; EJACULATION; HUMAN; MALE; PRIORITY JOURNAL; PROTEIN DEGRADATION; SEMINAL PLASMA; SEMINAL VESICLE;

ANTIBODY SPECIFICITY; EJACULATION; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; FIBRONECTINS; GONADAL STEROID HORMONES; HUMANS; HYDROLYSIS; KALLIKREINS; MALE; PROSTATE-SPECIFIC ANTIGEN; PROTEIN C INHIBITOR; SEMINAL VESICLE SECRETORY PROTEINS; SEMINAL VESICLES; SPERMATOZOA;

EID: 0030458254     PISSN: 01963635     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Akiyama K, Nakamura T, Invanaga S, Hara M. The chymotrypsin-like activity of human prostate-specific antigen γ-seminoprotein. FEBS Lett 1987;225:168-172.
2. Christensson A, Laurell CB, Lilja H. Enzymatic activity of prostate-specific antigen and its reactions with extracellular serine proteinase inhibitors. Eur J Biochem 1990;194:755-763.
3. Coleman PL, Latham HG Jr, Shaw EN. Some sensitive methods for the assay of trypsin-like enzymes. Methods Enzymol 1976;45:12-21.
4. Deperthes D, Chapdelaine P, Tremblay RR, Brunet C, Berton J, Hébert J, Lazure C, Dubé JY. Isolation of prostatic kallikrein hK2, also known as hGK-1, in human seminal plasma. Biochim Biophys Acta 1995;1245:311-316.
5. Espana F, Gilabert J, Estellés A, Romeu A, Aznar J, Cabo A. Functionally active protein C inhibitor/plasminogen activator inhibitor-3 (PCI/ PAI-3) is secreted in seminal vesicles, occurs at high concentrations in human seminal plasma and complexes with prostatic-specific antigen. Thromb Res 1991;64:309-320.
6. Fichtner J, Graves HCB, Thatcher K, Yemoto C, Shortliffe LM. Prostatespecific antigen releases a kinin-like substance on proteolysis of seminal vesicle fluid that stimulates smooth muscle contraction. J Urol 1996;155:738-742.
7. Garcia-Pardo A, Pearlstein E, Frangione B. Primary structure of human plasma fibronectin. J Biol Chem 1983;258:12670-12674.
8. Green GDJ, Shaw E. Thiobenzyl benzyloxycarbonyl-L-lysinate, substrate for a sensitive colorimetric assay for trypsin-like enzymes. Anal Biochem 1979;93:223-226.
9. Kornblihtt AR, Umezawa K, Vibe-Pedersen K, E Baralle E Primary structure of human fibronectin: differential splicing may generate at least 10 polypeptides from a single gene. EMBO J 1985;4:1755-1759.
10. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T-4. Nature 1970;227:680-685.
11. Lilja H. A kallikrein-like serine protease in prostatic fluid cleaves the predominant seminal vesicle protein. J Clin Invest 1985;76:1899-1903.
12. Lilja H, Abrahamsson PA, Lundwall A. Semenogelin, the predominant protein in human semen. J Biol Chem 1989;264:1894-1900.
13. Lilja H, Christensson A. Complex formation between protein C inhibitor and prostate-specific antigen in vitro and in human semen. Eur J Biochem 1994;220:45-53.
14. Lilja H, Lundwall A. Molecular cloning of epididymal and seminal vesicular transcripts encoding a semenogelin-related protein. Proc Natl Acad Sci USA 1992;89:4559-4563.
15. Lilja H, Oldbring J, Rannevik G, Laurell CB. Seminal vesicle-secreted proteins and their reactions during gelation and liquefaction of human semen. J Clin Invest 1987;80:281-285.
16. McGee RS, Herr JC. Human seminal vesicle-specific antigen is a substrate for prostate-specific antigen (or P-30). Biol Reprod 1988;39:499-510.
17. O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975;250:4007-4021.
18. Schedlich LJ, Bennets BH, Morris BJ. Primary structure of human glandular kallikrein gene. DNA 1987;6:429-437.
19. Wang MC, Valenzuela LA, Murphy GP, Chu TM. Purification of human prostatic specific antigen. Invest Urol 1979;17:159-163.