Direct imaging of DNA in living cells reveals the dynamics of chromosome formation

Journal of Cell Biology

Volumn 144, Issue 5, 1999, Pages 813-821

  Manders, Erik M M ^a,b   Kimura, Hiroshi ^a   Cook, Peter R ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Cell cycle; Chromosome; DNA replication; Fluorescein; Microscopy

Indexed keywords

THYMIDINE TRIPHOSPHATE;

ARTICLE; CELL CYCLE; CHROMOSOME; CHROMOSOME CONDENSATION; DNA DETERMINATION; DNA REPLICATION; DNA STRAND; MITOSIS; PRIORITY JOURNAL; SOMATIC CELL;

ANIMALS; CELLS, CULTURED; CHROMOSOMES; DEER; DNA; DNA REPLICATION; FLUORESCENT DYES; INTERPHASE; METAPHASE; MICROSCOPY, CONFOCAL;

EID: 0033535323     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.144.5.813     Document Type: Article

References (43)

1. Abney, J.R., B. Cutler, M.L. Fillbach, D. Axelrod, and B.A. Scalettar. 1997. Chromatin dynamics in interphase nuclei and its implications for nuclear structure. J. Cell Biol. 137:1459-1468.
2. Brakenhoff, G.J., M. Muller, and R.I. Ghauharali. 1996. Analysis of efficiency of two-photon versus single-photon absorption for fluorescence generation in biological objects. J. Microsc. 183:140-144.
3. Chalfie, M., Y. Tu, G. Euskirchen, W.W. Ward, and D.C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science. 263:802-805.
4. Cook, P.R. 1995. A chromomeric model for nuclear and chromosome structure. J. Cell Sci. 108:2927-2935.
5. Cremer, T., A. Kurz, R. Zirbel, S. Dietzel, B. Rinke, E. Schrock, M.R. Speicher, U. Mathieu, A. Jauch, P. Emmerich, et al. 1993. Role of chromosome territories in the functional compartmentalization of the cell nucleus. Cold Spring Harbor Symp. Quant. Biol. 58:777-792.
6. de With, A., and K.O. Greulich. 1995. Wavelength dependence of laser-induced DNA damage in lymphocytes observed by single-cell gel electrophoresis. J. Photochem. Photobiol. B. Biol. 30:71-76.
7. Denk, W., J.H. Strickler, and W.W. Webb. 1990. Two-photon laser scanning fluorescence microscopy. Science. 248:73-76.
8. Elledge, S.J. 1996. Cell cycle checkpoints: preventing an identity crisis. Science 274:1664-1672.
9. Friedberg, E.C. 1985. DNA Repair, W.H. Freeman, San Francisco, CA. 614 pp.
10. Griffin, B.A., S.R. Adams, and R.Y. Tsien. 1998. Specific covalent labeling of recombinant protein molecules inside living cells. Science. 281:269-272.
11. Hassan, A.B., and P.R. Cook. 1993. Visualization of replication sites in unfixed human cells. J. Cell Sci. 105:541-550.
12. Heald, R., R. Tournebize, T. Blank, R. Sandaltzoupoulos, P. Becker, A. Hyman, and E. Karsenti. 1996. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature. 382: 420-425.
13. Hiraoka, Y., J.S. Minden, J.R. Swedlow, J.W. Sedat, and D.A. Agard. 1989. Focal points for chromosome condensation and decondensation revealed by three-dimensional in vivo time-lapse microscopy. Nature. 342:293-296.
14. Hozák, P., A.B. Hassan, D.A. Jackson, and P.R. Cook. 1993. Visualization of replication factories attached to a nucleoskeleton. Cell. 73:361-373.
15. Jackson, D.A., and A. Pombo. 1998. Replicon clusters are stable units of chromosome structure: evidence that nuclear organization contributes to the efficient activation and propagation of S-phase in human cells. J. Cell Biol. 140: 1285-1295.
16. Jackson, D.A., A.S. Balajee, L. Mullenders, and P.R. Cook. 1994. Sites in human nuclei where DNA damaged by ultra-violet light is repaired: visualization and localization relative to the nucleoskeleton. J. Cell Sci. 107:1745-1752.
17. Kanda, T., K.F. Sullivan, and G.M. Wahl. 1998. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr. Biol. 8:377-385.
18. Leavitt, J., M. Fatone, C. Hestdalen, J.W. Obringer, and H.S. Tillinghast. 1997. Mutagenic activity of high-energy 532 ultra-short laser pulses. Radiat. Res. 147:490-494.
19. Li, G., G. Sudlow, and A.S. Belmont. 1998. Interphase cell cycle dynamics of a late-replicating heterochromatic homogeneously staining region: precise choreography of condensation/decondensation and nuclear positioning. J. Cell Biol. 140:975-989.
20. Manders, E.M., J. Stap, G.J. Brakenhoff, R. van Driel, and J.A. Aten. 1992. Dynamics of three-dimensional replication patterns during the S-phase, analyzed by double labelling of DNA and confocal microscopy. J. Cell Sci. 103: 857-862.
21. Manders, E.M., J. Stap, J. Strackee, R. van Driel, and J.A. Aten. 1996. Dynamic behavior of DNA replicating domains. Exp. Cell Res. 226:328-335.
22. Manuelidis, L. 1985. Individual interphase chromosome domains revealed by in situ hybridization. Hum. Genet. 71:288-293.
23. Marshall, W.F., A. Straight, J.F. Marko, J. Swedlow, A. Dernburg, A. Belmont. A.W. Murray, D.A. Agard, and J.W. Sedat. 1997. Interphase chromosomes undergo constrained diffusional motion in living cells. Curr. Biol. 7:930-939.
24. McNeil, P.L. 1989. Incorporation of macromolecules into living cells. Methods Cell Biol. 29:153-173.
25. Minden, J.S., D.A. Agard, J.W. Sedat, and B.M. Alberts. 1989. Direct cell lineage analysis in Drosophila melanogaster by time-lapse, three-dimensional optical microscopy of living embryos. J. Cell Biol. 109:505-516.
26. Misteli, T., J.F. Caceres, and D.L. Spector. 1997. The dynamics of pre-mRNA splicing factor in living cells. Nature. 387:523-527.
27. Nakamura, H., T. Morita, and C. Sato. 1986. Structural organizations of replicon domains during DNA synthetic phase in the mammalian nucleus. Exp. Cell Res. 165:291-297.
28. Nakayasu, H., and R. Berezney. 1989. Mapping replication sites in the eukaryotic cell nucleus. J. Cell Biol. 108:1-11.
29. Nickerson, J.A., B.J. Blencowe, and S. Penman. 1995. The architectural organization of nuclear metabolism. Int. Rev. Cytol. 162A:67-123.
30. O'Keefe, R.T., S.C. Henderson, and D.L. Spector. 1992. Dynamic organization of DNA replication in mammalian cell nuclei: spatial and temporal defined replication of chromosome specific α-satellite DNA sequences. J. Cell Biol. 116:1095-1110.
31. Paddy, M.R., H. Saumweber, D.A. Agard, and J.W. Sedat. 1996. Time-resolved, in vivo studies of mitotic spindle formation and nuclear lamina breakdown in Drosophila early embryos. J. Cell Sci. 109:591-607.
32. Park, P.C., and U. De Boni. 1991. Dynamics of nucleolar fusion in neuronal interphase nuclei in vitro: association with nuclear rotation. Exp. Cell Res. 197: 213-221.
33. Pillidge, L., C.S. Downes, and R.T. Johnson. 1986. Defective post-replication recovery and uv sensitivity in a simian virus 40-transformed Indian muntjac cell line. Int. J. Radiat. Biol. 50:119-136.
34. Robinett, C.C., A. Straight, G. Li, C. Willhelm, G. Sudlow, A. Murray, and A.S. Belmont. 1996. In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition. J. Cell Biol. 135:1685-1700.
35. Ryan, A.J., and R.T. Johnson. 1996. Dominant genetic instability and sensitivity to DNA damaging agents in a mammalian cell line. Somat. Cell Mol. Genet. 22:177-189.
36. Selvin, P.R. 1995. Fluorescence resonance energy transfer. Methods Enzymol. 246:300-334.
37. Shelby, R.D., K.M. Hahn, and K.F. Sullivan. 1996. Dynamic elastic behavior of α-satellite DNA domains visualized in situ in living human cells. J. Cell Biol. 135:545-557.
38. Straight, A.F., A.S. Belmont, C.C. Robinett, and A.W. Murray. 1996. GFP tagging of budding yeast chromosomes reveals that protein-protein interactions can mediate sister chromatid cohesion. Curr. Biol. 6:1599-1608.
39. Trask, B.J., S. Allen, H. Massa, A. Fertitta, R. Sachs, G. van den Engh, and M. Wu. 1993. Studies of metaphase and interphase chromosomes using fluorescence in situ hybridization. Cold Spring Harbor Symp. Quant. Biol. 58:767-775.
40. Tsien, R.Y., and A. Miyawaki. 1998. Seeing the machinery of live cells. Science. 280:1954-1955.
41. Yu, H., J. Chao, D. Patek, R. Mujumdar, S. Mujumdar, and A.S. Waggoner. 1994. Cyanine dye dUTP analogs for enzymatic labeling of DNA probes. Nucleic Acids Res. 22:3226-3232.
42. Zhang, D., and R.B. Nicklas. 1996. 'Anaphase' and cytokinesis in the absence of chromosomes. Nature. 382:466-468.
43. Zink, D., T. Cremer, R. Saffrich, R. Fischer, M.F. Trendelenburg, W. Ansorge, and E.H.K. Stelzer. 1998. Structure and dynamics of human interphase chromosome territories in vivo. Hum. Genet. 102:241-251.