Isolation and characterization of an oligomerization-negative mutant of HIV-1 integrase

Virology

Volumn 259, Issue 2, 1999, Pages 274-285

  Kalpana, Ganjam V ^a   Reicin, Alise ^b   Cheng, Grace S W ^a   Sorin, Mariya ^a   Paik, Sandra ^b   Goff, Stephen P ^b  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

^b College of Physicians and Surgeons ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTEGRASE;

AMINO ACID SUBSTITUTION; ARTICLE; CARBOXY TERMINAL SEQUENCE; DNA INTEGRATION; ENZYME ISOLATION; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; LEUKEMIA CELL LINE; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; VIRUS INFECTIVITY; VIRUS MUTANT; VIRUS REPLICATION;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 0033526768     PISSN: 00426822     EISSN: None     Source Type: Journal    
DOI: 10.1006/viro.1999.9767     Document Type: Article

References (63)

1. Aldovini A., Feinberg M. B. Transfection of molecularly cloned HIV genomes. Aldovini A., Walker B. Techniques in HIV Research. 1990;Stockton Press, New York.
2. Andrake M. D., Skalka A. M. Multimerization determinants reside in both the catalytic core and C-terminus of ASV integrase. J. Biol. Chem. 270:1995;29299-29306.
3. Andrake M. D., Skalka A. M. Retroviral integrase: Putting the pieces together. J. Biol. Chem. 271:1996;19633-19636.
4. Asante-Appiah E., Skalka A. M. Molecular mechanisms in retrovirus DNA integration. Antiviral Res. 36:1997;139-156.
5. Barsov E. V., Huber W. E., Marcotrigiano J., Clark P. K., Clark A. D., Arnold E., Hughes S. H. Inhibition of human immunodeficiency virus type 1 integrase by the Fab fragment of a specific monoclonal antibody suggests that different multimerization states are required for different enzymatic functions. J. Virol. 70:1996;4484-4494.
6. Brachmann R. K., Boeke J. D. Tag games in yeast: The two-hybrid system and beyond. Curr. Opin. Biotechnol. 8:1997;561-568.
7. Brown P. O., Bowerman B., Varmus H. E., Bishop J. M. Correct integration of retroviral DNA in vitro. Cell. 49:1987;347-356.
8. Brown P. O., Bowerman B., Varmus H. E., Bishop J. M. Retroviral integration: Structure of the initial covalent product and its precursor, and a role for the viral integrase protein. Proc. Natl. Acad. Sci. USA. 86:1989;2525-2529.
9. Bukrinsky M. I., Sharova N., Dempsey M. P., Stanwick T. L., Bukrinskaya A. S., Haggerty S., Stevenson M. Active nuclear import of human immunodeficiency virus type 1 preintegration complexes. Proc. Natl. Acad. Sci. USA. 89:1992;6580-6584.
10. Bushman F. D., Craigie R. Activities of human immunodeficiency virus (HIV) integration protein in vitro: Specific cleavage and integration of HIV DNA. Proc. Natl. Acad. Sci. USA. 88:1991;1339-1343.
11. Bushman F. D., Engelman A., Palmer I., Wingfield P., Craigie R. Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. Proc. Natl. Acad. Sci. USA. 90:1993;3428-3432.
12. Bushman F. D., Fujiwara T., Craigie R. Retroviral DNA integration directed by HIV integration protein in vitro. Science. 249:1990;1555-1558.
13. Cai M., Zheng R., Caffrey M., Craigie R., Clore G. M., Gronenborn A. M. Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. Nat. Struct. Biol. 4:1997;567-577.
14. Cannon P. M., Wilson W., Byles E., Kingsman S. M., Kingsman A. J. Human immunodeficiency virus type 1 integrase: Effect on viral replication of mutations at highly conserved residues. J. Virol. 68:1994;4768-4775.
15. Chien C.-T., Bartel P. L., Sternglanz R., Fields S. The two hybrid system: A method to identify and clone genes for proteins that interact with a protein of interest. Proc. Natl. Acad. Sci. USA. 88:1991;9578-9582.
16. Chow S. A., Vincent K. A., Ellison V., Brown P. O. Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus. Science. 255:1992;723-726.
17. Craigie R., Fujiwara T., Bushman F. The IN protein of Moloney murine leukemia virus processes the viral DNA ends and accomplishes their integration in vitro. Cell. 62:1990;829-837.
18. Drelich M., Wilhelm R., Mous J. Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 IN protein in vitro. Virology. 188:1992;459-468.
19. Dyda F., Hickman A. B., Jenkins T. M., Engelman A., Craigie R., Davies D. R. Crystal structure of the catalytic domain of HIV-1 integrase: Similarity to other polynucleotidyl transferases. Science. 266:1994;1981-1986.
20. Eijkelenboom A. P. A. M., Lutzke R. A. P., Boelens R., Plasterk R., Kaptein R., Hard K. The DNA-binding domain of HIV-1 integrase has an SH3-like fold. Nat. Struct. Biol. 2:1995;807-810.
21. Ellison V., Gerton J., Vincent K. A., Brown P. O. An essential interaction between distinct domains of HIV-1 integrase mediates assembly of the active multimer. J. Biol. Chem. 270:1995;3320-3326.
22. Engelman A., Bushman F. D., Craigie R. Identification of discrete functional domains of HIV-1 integrase and their organization within an active multimeric complex. EMBO J. 12:1993;3269-3275.
23. Engelman A., Craigie R. Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro. J. Virol. 66:1992;6361-6369.
24. Engelman A., Mizuuchi K., Craigie R. HIV-1 DNA integration: Mechanism of viral DNA cleavage and DNA strand transfer. Cell. 67:1991;1211-1221.
25. Fields S., Song O.-K. A novel genetic system to detect protein-protein interactions. Nature. 340:1989;245-246.
26. Fujiwara T., Craigie R. Integration of mini-retroviral DNA: A cell-free reaction for biochemical analysis of retroviral integration. Proc. Natl. Acad. Sci. USA. 86:1989;3065-3069.
27. Fujiwara T., Mizuuchi K. Retroviral DNA integration: Structure of an integration intermediate. Cell. 54:1988;497-504.
28. Gill G., Ptashne M. Mutants in GAL4 protein altered in an activation function. Cell. 51:1987;121-126.
29. Goff S., Traktman P., Baltimore D. Isolation and properties of Moloney murine leukemia virus mutants: Use of a rapid assay for release of virion reverse transcriptase. J. Virol. 38:1981;239-248.
30. Goff S. P. Genetics of retroviral integration. Annu. Rev. Genet. 26:1992;527-544.
31. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 52:1973;456-471.
32. Heuer T. S., Brown P. O. Mapping features of HIV-1 integrase near selected sites on viral and target DNA molecules in an active enzyme-DNA complex by photo-crosslinking. Biochemistry. 36:1997;10655-10665.
33. Heuer T. S., Brown P. O. Photo-crosslinking studies suggest a model for the architecture of an active human immunodeficiency virus type 1 integrase-DNA complex. Biochemistry. 37:1998;6667-6678.
34. Jenkins T. M., Engelman A., Ghirlando R., Craigie R. A soluble active mutant of HIV-1 integrase: Involvement of both the core and carboxyl-terminal domains in multimerization. J. Biol. Chem. 271:1996;7712-7718.
35. Jones K. S., Coleman J., Merkel G. W., Lauel T. M., Skalka A. M. Retroviral integrase functions as a multimer and can turn over catalytically. J. Biol. Chem. 267:1992;16037-16040.
36. Kalpana G. V., Goff S. P. Genetic analysis of homomeric interactions of human immunodeficiency virus type 1 integrase using the yeast two-hybrid system. Proc. Natl. Acad. Sci. USA. 90:1993;10593-10597.
37. Kalpana G. V., Marmon S., Wang W., Crabtree G. R., Goff S. P. Binding and stimulation of HIV-1 integrase by a human homolog of yeast transcription factor SNF5. Science. 266:1994;2002-2006.
38. Katz R. A., Merkel G., Kulkovsky J., Leis J., Skalka A. M. The avian retroviral IN protein is both necessary and sufficient for integrative recombination in vitro. Cell. 63:1990;87-95.
39. Katzman M., Katz R. A., Skalka A. M., Leis J. The avian retroviral integration protein cleaves the terminal sequences of linear viral DNA at the in vivo sites of integration. J. Virol. 63:1989;5319-5327.
40. Khan E., Mack J. P. G., Katz R. A., Kulkosky J., Skalka A. M. Retroviral integrase domains: DNA binding and the recognition of LTR sequences. Nucleic Acids Res. 19:1991;851-860.
41. Kulkosky J., Jones K. S., Katz R. A., Mack J. P. G., Skalka A. M. Residues critical for retroviral integrative recombination in a region that is highly conserved among retroviral/retrotransposon integrases and bacterial insertion sequence transposases. Mol. Cell. Biol. 12:1992;2331-2338.
42. LaFemina R. L., Schneider C. L., Robbins H. L., Callahan P. L., LeGrow K., Roth E., Schleif W. A., Emini E. A. Requirement of active human immunodeficiency virus type 1 integrase enzyme for productive infection of human T-lymphoid cells. J. Virol. 66:1992;7414-7419.
43. Leavitt A. D., Shiue L., Varmus H. E. Site-directed mutagenesis of HIV-1 integrase demonstrates differential effects on integrase functions in vitro. J. Biol. Chem. 268:1993;2113-2119.
44. 2+ promotes the self-association of human immunodeficiency virus type 1 integrase in vitro. Biochemistry. 36:1997;173-180.
45. Lobel L. I., Goff S. P. Construction of mutants of Moloney murine leukemia virus by suppressor-linker insertion mutagenesis: Positions of viable insertion mutations. Proc. Natl. Acad. Sci. USA. 81:1984;4149-4153.
46. Lodi P. J., Ernst J. A., Kuszewski J., Hickman A. B., Engelman A., Craigie R., Clore G. M., Gronenborn A. M. Solution structure of the DNA binding domain of HIV-1 integrase. Biochemistry. 34:1995;9826-9833.
47. McCutchan J. H., Pagano J. S. Enhancement of infectivity of SV40 deoxyribonucleic acid with DEAE dextran. J. Natl. Cancer Inst. 41:1968;351-357.
48. Prasad V. R., Lowy I., De Los Santos T., Chiang L., Goff S. P. Isolation and characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase. Proc. Natl. Acad. Sci. USA. 88:1991;11363-11367.
49. Puras Lutzke R. A., Plasterk R. H. Structure-based mutational analysis of the C-terminal DNA-binding domain of human immunodeficiency virus type 1 integrase: Critical residues for protein oligomerization and DNA binding. J. Virol. 72:1998;4841-4848.
50. Ratner L., Haseltine W., Patarca R., Livak K. J., Starcich B., Joseph S. F., Doran E. R., Rafalski J. A., Whitwhoen E. A., Baumeister K., Ivanoff L., Petteway S. R., Pearson M. L., Lautenberger J. A., Papas T. S., Ghrayeb J., Chang N. T., Gallo R. C., Wong-Staal F. Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature. 313:1985;277-284.
51. Reicin A. S., Paik S., Berkowitz R. D., Luban J., Lowy I., Goff S. P. Linker insertion mutations in the human immunodeficiency virus type 1 gag gene: Effect on virion particle assembly, release, and infectivity. J. Virol. 69:1995;642-650.
52. Sakai H., Kawamura M., Sakuragi J., Sakuragi S., Shibata R., Ishimoto A., Ono N., Ueda S., Adachi A. Integration is essential for efficient gene expression of human immunodeficiency virus type 1. J. Virol. 67:1993;1169-1174.
53. Shin C.-G., Taddeo B., Haseltine W. A., Farnet C. M. Genetic analysis of the human immunodeficiency virus type 1 integrase protein. J. Virol. 64:1994;2421-2425.
54. van Gent D. C., Elgersma Y., Bolk M. W. J., Vink C., Plasterk R. H. A. DNA binding properties of the integrase proteins of human immunodeficiency viruses types 1 and 2. Nucleic Acids. Res. 19:1991;3821-3827.
55. van Gent D. C., Vink C., Oude Groeneger A. A. M., Plasterk R. H. A. Complementation between HIV integrase proteins mutated in different domains. EMBO J. 12:1993;3261-3267.
56. Vidal M., Brachmann R. K., Fattaey A., Harlow E., Boeke J. D. Reverse two-hybrid and one-hybrid systems to detect dissociation of protein-protein and DNA-protein interactions. Proc. Natl. Acad. Sci. USA. 93:1996;10315-10320.
57. Vincent K. A., Ellison V., Chow S. A., Brown P. O. Characterization of human immunodeficiency virus type 1 integrase expressed in Escherichia coli and analysis of variants with amino-terminal mutations. J. Virol. 67:1993;425-437.
58. Vink C., Oude Groeneger A. A. M., Plasterk R. H. A. Identification of the catalytic and DNA-binding region of the human immunodeficiency virus type 1 integrase protein. Nucleic Acids Res. 21:1993;1419-1425.
59. Vink C., Plasterk R. H. A. The human immunodeficiency virus integrase protein. Trends Genet. 9:1994;433-437.
60. White M. A. The yeast two-hybrid system: Forward and reverse. Proc. Natl. Acad. Sci. USA. 93:1996;10001-10003.
61. Wiskerchen M., Muesing M. A. Human immunodeficiency virus type 1 integrase: Effects of mutations on viral ability to integrate, direct viral gene expression from unintegrated viral DNA templates, and sustain viral propagation in primary cells. J. Virol. 69:1995;376-386.
62. Wolfe A. L., Felock P. J., Hastings J. C., Blau C. U., Hazuda D. J. The role of manganese in promoting multimerization and assembly of human immunodeficiency virus type 1 integrase as a catalytically active complex on immobilized long terminal repeat substrates. J. Virol. 70:1996;1424-1432.
63. Zheng R., Jenkins T. M., Craigie R. Zinc folds the N-terminal domain of HIV-1 integrase, promotes multimerization, and enhances catalytic activity. Proc. Natl. Acad. Sci. USA. 93:1996;13659-13664.