Synthesis of biotechnologically relevant heterologous proteins in filamentous fungi

Process Biochemistry

Volumn 32, Issue 6, 1997, Pages 529-539

  Radzio, Renate ^a   Kück, Ulrich ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

Expression system; Filamentous fungi; Heterologous proteins; Pathway engineering; Protein production; Secretion

Indexed keywords

ARTICLE; ASPERGILLUS NIGER; ASPERGILLUS ORYZAE; CEPHALOSPORIUM ACREMONIUM; EUKARYOTE; GENETIC ENGINEERING; PENICILLIUM CHRYSOGENUM; PROTEIN MODIFICATION; PROTEIN PROCESSING; TRICHODERMA REESEI;

EID: 0343924362     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0032-9592(97)00004-6     Document Type: Article

References (84)

1. Demain, A. L., Kimura, A. and Shinmyo, A., Contribution of recombinant microbes and their potentials. In Recombinant Microbes for Industrial and Agricultural Applications, eds Y. Murooka and T. Imanaka. Marcel Dekker, New York, 1994, pp. 27-46.
2. Lemke, P. A. and Peng, M., Genetic manipulation of fungi by DNA-mediated transformation. In The Mycota II, ed. U. Kiick. Springer Press, Berlin, 1995, pp. 109-139.
3. Finkelstein, D. B., Transformation. In Biotechnology of Filamentous Fungi: Technology Acid Products, eds D. B. Finkelstein and C. Ball. Butterworth-Heinemann, London, 1992, pp. 113-156.
4. Fincham, J. R. S., Transformation in fungi. Microbiological Reviews, 1989, 53, 148-170.
5. Jeenes, D. J., MacKenzie, D. A., Roberts, I. N. and Archer, D. B., Heterologous protein production by filamentous fungi, Biotechnology and Genetic Engineering Reviews, 1991, 9, 327-367.
6. van den Hondel, C. A. M. J. J., Punt, P. J. and van Gorcom, R. F. M., Heterologous gene expression in filamentous fungi. In More Gene Manipulations in Fungi, eds J. W. Bennett and L. L. Lasure. Academic Press, San Diego, CA, 1991, pp. 396-428.
7. Saunders, G., Picknett, T. M., Tuite, M. F. and Ward, M., Heterologous gene expression in filamentous fungi. Trends in Biotechnology, 1989, 7, 283-287.
8. Kolar, M., Punt, P. J., van den Hondel, C. A. M. J. J. and Schwab, H., Transformation of Penicillium chrysogenum using dominant selection markers and expression of an Escherichia coli lacZ fusion gene. Gene, 1988, 62, 127-134.
9. Punt, P. J., Zegers, N. D., Busscher, M., Pouwels, P. H. and van den Hondel, C. A. M. J. J., Intracellular and extracellular production of proteins under the control of expression signals of the highly expressed Aspergillus nidulans gpdA gene. Journal of Biotechnology, 1991, 17, 19-34.
10. Archer, D. B., Jeenes, D. J., MacKenzie, D. A., Brightwell, M., Lambert, N., Lowe, G., Radford, S. E. and Dobson, C. M., Hen egg white lysozyme expressed in and secreted from Aspergillus niger is correctly processed and folded. Bio/Technology, 1990, 8, 741-745.
11. Ward, M., Production of calf chymosin by Aspergillus awamori. In Genetics and Molecular Biology of Industrial Microorganisms, eds C. L. Hershberger, S. W. Queener and G. Hegeman. American Society for Microbiology, Washington DC, 1989, pp. 288-294.
12. Archer, D. B., Enzyme production by recombinant Aspergillus. In Recombinant Microbes for Industrial and Agricultural Applications, eds Y. Murooka and T. Imanaka. Marcel Dekker, New York, 1994, pp. 373-393.
13. Ross, J., Control of messenger RNA stability in higher eukaryotes. Trends in Genetics, 1996, 12, 171-175.
14. Platt, A., Langdon, T., Arst, H. N., Kirk, D., Tollervey, D., Mates Sanchez, J. M. and Caddick, M. X., Nitrogen metabolite signalling involves the C-terminus and the GATA domain of the Aspergillus transcription factor AREA and the 3′ untranslated region of its mRNA. EMBO Journal, 1996, 15, 2791-2801.
15. Schatz, G. and Dobberstein, B., Common principles of protein translocation across membranes. Science, 1996, 271, 1519-1526.
16. Punt, P. J., Veldhuisen, G. and an den Hondel, C. A. M. J. J., Protein targeting and secretion in filamentous fungi. Antonie van Leuwenhoek, 1994, 65, 211-216.
17. Ward, M., Wilson, L. J., Kodama, K. H., Rey, M. W. and Berka, R. M., Improved production of chymosin in Aspergillus by expression of glucoamylase-chymosin fusion. Bio/Technology, 1990, 8, 435-438.
18. Contreras, R., Carrez, D., Kinghorn, J. R., van den Hondel, C. A. M. J. J. and Fiers, W., Efficient KEX2-like processing of a glucoamylase-interleukin-6 fusion protein by Aspergillus nidulans and secretion of mature interleukin-6. Bio/Technology, 1991, 9, 378-381.
19. Peberdy, J. F., Protein secretion in filamentous fungi-trying to understand a highly productive black box. Trends in Biotechnology, 1994, 12, 50-57.
20. Hartl, F. U., Molecular chaperones in cellular protein folding. Nature, 1996, 381, 571-580.
21. Punt, P. J., Veldhuisen, G., Kuijvenhoven, A. and van den Hondel, C. A. M. J. J., Towards an analysis system of the secretion pathway of Aspergillus niger. Fungal Genetics Newsletter, 1996, 43B, 26.
22. Jeenes, D., Ngiam, C. and Archer, D. B., Isolation and characterisation of PDI-family genes from Aspergillus niger. Fungal Genetics Newsletter, 1996, 43B, 23.
23. Dalbey, R. E. and von Heijne, G., Signal peptidase in prokaryotes and eukaryotes - a new protease family. Trends in Biochemistry, 1992, 17, 474-478.
24. Jenkins, N., Parekh, R. B. and James, D. C., Getting the glycosylation right: implications for the biotechnology industry. Nature Biotechnology, 1996, 14, 975-981.
25. Dunn-Coleman, N. S., Bloebaum, P., Barka, M., Bodie, E., Robinson, N., Armstrong, G., Ward, M., Przetak, M., Carter, G. L., Lamsa, M. and Hiensohn, H., Commercial levels of chymosin production by Aspergillus. Mol. Gen. Genet., 1991, 230, 288-294.
26. Mattern, I. E., van Noort, J. M., van den Berg, P., Archer, D. B., Robert, I. N. and van den Hondel, C. A. M. J. J., Isolation and characterization of mutants of Aspergillus niger which are deficient in extracellular proteases. Mol. Gen. Genet., 1992, 234, 332-337.
27. Katz, M. E., Flynn, P. K., van Kuyk, P. A. and Cheetham, B. F., Mutations affecting extracellular protease production in the filamentous fungus Aspergillus nidulans. Mol. Gen. Genet., 1996, 250, 715-724.
28. Berka, R. M., Ward, M., Wilson, L. J., Hayenga, K. J., Kodama, K. H., Carlomagno, L. P. and Thompson, S. A., Molecular cloning and deletion of the aspergillopepsin A gene from Aspergillus awamori. Gene, 1990, 86, 153-157.
29. Verdoes J. C., Punt, P. J., Stouthamer, A. H. and van den Hondel, C. A. M. J. J., The effect of multiple copies of the upstream region on expression of the Aspergillus niger glucoamylase-encoding gene. Gene, 1994, 145, 179-187.
30. Gwynne, D. I., Foreign proteins. In Applied Molecular Genetics of Filamentous Fungi, eds J. B. Kinghorn and G. Turner. Blackie and Son Ltd, Glasgow, 1992, pp. 132-151.
31. Skatrud, P. L., Queener, S. W., Carr, L. G. and Fisher, D. L., Efficient integrative transformation of Cephalosporium acremonium. Current Genetics, 1987, 12, 337-348.
32. Picknett, T. M., Saunders, G., Ford, P. and Hold, G., Development of a gene transformation system for Penicillium chrysogenum. Current Genetics, 1987, 12, 449-452.
33. Skatrud, P. L., Genetic engineering of β-lactam antibiotic biosynthetic pathways in filamentous fungi. Trends in Biotechnology, 1992, 10, 324-329.
34. Smith, D. J., Bull, J. H., Edward, J. and Turner, G., Amplification of the isopenicillin N synthetase gene in a strain of Penicillium chrysogenum producing high levels of penicillin. Mol. Gen. Genet., 1989, 216, 492-498.
35. Fernández-Canõn, J. M. and Penãlva, M. A., Overexpression of two penicillin structural genes in Aspergillus nidulans. Mol. Gen. Genet., 1995, 246, 110-118.
36. Skatrud, P. L., Tietz, A. J., Ingolia, T. D., Cantwell, C. A., Fisher, D. L., Chapman, J. L. and Queener, S. W., Use of recombinant DNA to improve production of cephalosporin C by Cephalosporium acremonium. Bio/Technology, 1989, 7, 477-485.
37. Isogai, T., Fukagawa, M., Aramori, I., Iwami, M., Kojo, H., Ono, T., Ueda, Y., Kohsaka, M. and Imanaka, H., Construction of a 7-aminocephalosporanic acid (7-ACA) biosynthesis operon and direct production of 7-ACA in Acremonium chrysogenum. Bio/Technology, 1991, 9, 188-191.
38. Crawford, L., Stepan, A. M., McAda, P. C., Rambosek, J. A., Conder, M. J., Vinci, V. A. and Reeves, C. D., Production of cephalosporin intermediates by feeding adipic acid to recombinant Penicillium chrysogenum strains expressing ring expansion activity. Bio/Technology, 1995, 13, 58-62.
39. Gutierrez, S., Diez, B., Alvarez, E., Barredo, J. L. and Martin, J. F., Expression of the penDE gene of Penicillium chrysogenum in Cephalosporium acremonium: production of benzylpenicillin by the transformants. Mol. Gen. Genet., 1991, 225, 56-64.
40. De Modena, J. A., Gutierrez, S., Velasco, J., Fernandez, F. J., Fachini, R. A., Galazzo, J. L., Hughes, D. E. and Martin, J. F., The production of cephalosporin C by Acremonium chrysogenum is improved by the intracellular expression of a bacterial hemoglobin. Bio/Technology, 1993, 11, 926-929.
41. Roberts, I. N., Oliver, R. P., Punt, P. J. and van den Hondel, C. A. M. J. J., Expression of the Escherichia coli β-glucuronidase gene in industrial and phytopathogenic filamentous fungi. Current Genetics, 1989, 15, 177-180.
42. Punt, P. J., Greaves, P. A., Kuyvenhoven, A., van Deutekorn, J. C. T., Kinghorn, J. R., Pouwels, P. H. and van den Hondel, C. A. M. J. J., A twin-reporter vector for simultaneous analysis of expression signals of divergently transcribed, contiguous genes in filamentous fungi. Gene, 1991, 104, 119-122.
43. Gwynne, D. I., Buxton, F. P., Williams, S. A., Garven, S. and Davies, R. W., Genetically engineered secretion of active human interferon and a bacterial endoglucanase from Aspergillus nidulans. Bio/Technology, 1989, 5, 713-719.
44. MacRae, W. D., Buxton, F. P., Gwynne, D. I. and Davies, R. W., Heterologous protein secretion directed by a repressible acid phosphatase system of Aspergillus niger. Gene, 1993, 132, 193-198.
45. Carrez, D., Janssens, W., Degrave, P., van den Hondel, C. A. M. J. J., Kinghorn, J. R., Fiers, W. and Contreras, R., Heterologous gene expression by filamentous fungi: secretion of human interleukin-6 by Aspergillus nidulans. Gene, 1990, 94, 147-154.
46. Ward, P. P., Piddington, C. S., Cunningham, G. A., Zhou, X., Wyatt, R. D. and Conneely, O. M., A system for production of commercial quantities of human lactoferrin: a broad spectrum natural antibiotic. Bio/Technology, 1995, 13, 498-503.
47. Mestric, S., Punt, P. J., Valinger, R. and van den Hondel, C. A. M. J. J., Expression of human insulin gene in Aspergillus niger. Fungal Genetics Newsletter, 1996, 43B, 25.
48. Nyyssönen, E., Penttilä, M., Harkki, A., Saloheimo, A., Knowles, J. K. and Keränen, S., Efficient production of antibody fragments by the filamentous fungus Trichoderma reesei. Biotechnology (NY), 1993, 11, 591-595.
49. Nyyssönen, E. and Keränen, S., Multiple roles of the cellulase CBHI in enhancing production of fusion antibodies by the filamentous fungus Trichoderma reesei. Current Genetics, 1995, 28, 71-79.
50. Archer, D. P., Jeenes, D. J. and Mackenzien, D. A., Strategies for improving heterologous protein production from filamentous fungi. Antonie van Leeuwenhoek, 1994, 65, 245-250.
51. Stief, A., Winter, D. M., Strätling, W. H. and Sippel, A. E., A nuclear DNA attachment element mediates elevated and position-independent activity. Nature, 1989, 341, 343-345.
52. Allen, G. C., Hall, G. E., Childs, L. C., Wlisinger, A. K., Spiker, S. and Thompson, W. F., Scaffold attachment regions increase reporter gene expression in stably transformed plant cells. Plant Cell, 1993, 5, 603-613.
53. Kalsner, I., Hintz, W., Reid, L. S. and Schachter, H., Insertion into Aspergillus nidulans of functional UDP-GlcNAc: alpha 3-D-mannoside β-1,2-N-acetylglucosaminyl-transferase I, the enzyme catalysing the first committed step from oligomannose to hybrid and complex N-glycans. Glycoconjugation Journal, 1995, 12, 360-370.
54. Menne, S., Walz, M. and Kück, U., Expression studies with the bidirectional pcbAB - pcbC promoter region from Acremonium chtysogenum using reporter gene fusions. Applied Microbiology and Biotechnology, 1994, 42, 57-66.
55. Punt, P. J., Dingemanse, M. A., Kuyvenhoven, A., Soede, R. D., Pouwels, P. H. and van den Hondel, C. A. M. J. J., Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase. Gene, 1990, 93, 101-109.
56. Brakhage, A. A., Browne, P. and Turner, G., Regulation of Aspergillus nidulans penicillin biosynthesis genes acvA and ipnA by glucose. Journal of Bacteriology, 1992, 174, 3789-3799.
57. Then Bergh, K., Litzka, O. and Brakhage, A, A., Identification of a major cis-acting DNA element controlling the bidirectionally transcribed penicillin biosynthesis genes acvA (pcbAB) and ipnA (pcbC) of Aspergillus nidulans. Journal of Bacteriology, 1996, 178, 3908-3916.
58. Pérez-Esteban, B., Gomez-Pardo, E. and Penãlva, M. A., A lacZ reporter fusion method for the genetic analysis of regulatory mutation in pathways of fungal secondary metabolism and its application to the Aspergillus nidulans penicillin pathway. Journal of Bacteriology, 1995, 177, 6069-6076.
59. Brakhage, A. A. and van den Brulle, J., Use of reporter genes to identify recessive trans-acting mutations specifically involved in the regulation of Aspergillus nidulans penicillin biosynthesis genes. Journal of Bacteriology, 1995, 177, 2781-2788.
60. Litzka, O., Then Bergh, K. and Brakhage, A. A., Analysis of the regulation of the Aspergillus nidulans penicillin biosynthesis gene aat (penDE), which encodes acyl coenzyme A: 6-amino-penicillanic acid acyltransferase. Mol. Gen. Genet., 1995, 249, 557-569.
61. Litzka, O., Then Bergh, K. and Brakhage, A. A., The Aspergillils nidulans penicillin-biosynthesis gene aat (penDE) is controlled by a CCAAT-containing DNA element. European Journal of Biochemistry, 1996, 238, 675-682.
62. Punt, P. J., Strauss, J., Smit, R., Kinghorn, J. R., van den Hondel, C. A. M. J. J. and Scazzocchio, C., The intergenic region between the divergently transcribed niiA and niaD genes of Aspergillus nidulans contains multiple NIRA binding sites which act bidirectionally. Molecular and Cellular Biology, 1995, 15, 5688-5699.
63. Gouka, R. J., Hessing, J. G. M., Punt, P. J., Stam, H., Musters, W. and van den Hondel, C. A. M. J. J., An expression system based on the promoter region of the Aspergillus awamori 1,4-β-endoxylanase A gene. Applied Microbiology and Biotechnology, 1996, 46, 28-35.
64. Tada, S., Gomi, K., Kitamoto, K., Takahashi, K., Tamura, G. and Hara, S., Construction of a fusion gene comprising the taka-amylase A promoter and the Escherichia colt β-glucuronidase gene and analysis of its expression in Aspergillus oryzae. Mol. Gen. Genet., 1991, 229, 301-306.
65. Bell-Pedersen, D., Dunlap, J. C. and Loros, J. J., Distinct cis-acting elements mediate clock, light and development regulation of the Neurospora crassa eas (ccg-2) gene. Molecular and Cellular Biology, 1996, 16, 513-521.
66. Kolar, M., Holzmann, K., Weber, G., Leitner, E. and Schwab, H., Molecular characterization and functional analysis in Aspergillus nidulans of the 5′-region of the Penicillium chrysogenum isopenicillin N synthetase gene. Bio/Technology, 1991, 17, 67-80.
67. Feng, B., Friedlin, E. and Marzluf, G. A., A reporter gene analysis of penicillin biosynthesis gene expression in Penicillium chrysogenum and its regulation by nitrogen and glucose catabolite repression. Applied and Environmental Microbiology, 1994, 60, 4432-4439.
68. Stangl, H., Gruber, F. and Kubicek, C. P., Characterization of the Trichoderma reesei cbh2 promoter. Current Genetics, 1993, 23, 115-122.
69. Morita, S., Kuriyama, M., Nakatsu, M. and Kitano, K., High level expression of Fusarium alkaline protease gene in Acremonium chrysogenum. Bioscience Biotechnology and Biochemistry, 1994, 58, 627-630.
70. Filipphi, M. J. A., Visser, J., van der Veen, P. and De Graaff, L. H., Cloning of the Aspergillus niger gene encoding α-L-arabinofuranosidase A. Applied Microbiology and Biotechnology, 1993, 39, 335-340.
71. Ward, M., Wilson, L. J. and Kodama, K. H., Use of Aspergillus over-producing mutants, cured for integrated plasmid, to overproduce heterologous proteins. Applied Microbiology Biotechnology, 1993, 39, 738-743.
72. Murakami, K., Aikawa, J., Horinouchi, S., Beppu, T., Characterization of an aspartic proteinase of Mucor pusillus expressed in Aspergillus oryzae. Mol. Gen. Genet., 1993, 241, 312-318.
73. Tsuchiya, K., Gomi, K., Kitamoto, K., Kumagai, C. and Tamura, G., Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae. Applied Microbiology and Biotechnology, 1993, 40, 327-332.
74. van Gemeren, I. A., Beijersbergen, A., Musters, W., Gouka, R. J., van den Hondel, C. A. M. J. J. and Verrips, C. T., The effect of pre-and prosequences and multicopy integration on heterologous expression of the Fusarium solani pisi cutinase gene in Aspergilllis awamori. Applied Microbiology and Biotechnology, 1996, 45, 755-763.
75. Gouka, R. J., Punt, P. J., Hessing, J. G. M. and van den Hondel, C. A. M. J. J., Analysis of heterologous protein production in defined recombinant Aspergillus awamori strains. Applied and Environmental Microbiology, 1996, 62, 1951-1957.
76. Hessing, H. J. G. M., Frenken, L. G. J., van Muijewijk-Harteveld, M., Musters, W. and van den Hondel, C. A. M. J. J., Studies on the production of single-chain antibody fragments by Aspergillus species. Fungal Genetics Newsletter, 1996, 43B, 23.
77. Finkelstein, D. B., Rambosek, J., Crawford, M. S., Soliday, C. L., McAda, P. C. and Leach, J., Protein secretion in Aspergillus niger. In Genetics and Molecular Biology of Industrial Microorganisms, eds C. L. Hershberger, S. W. Queener and G. Hegman. American Society for Microbiology, Washington DC, 1989, pp. 295-300.
78. Dewhand, M., Williams, S. A., Johnstone, J. A., Gwynne, D., Buxton, F. P. and Davies, R. W., Expression systems from Aspergillus nidulans. In Genetics and Molecular Biology of Industrial Microorganisms, eds C. L. Hershberger, S. W. Queener and G. Hegeman. American Society for Microbiology, Washington DC, 1989, pp. 301-303.
79. Joutsjoki, V. V., Construction by one-step gene replacement of Trichoderma reesei strains that produce the glucoamylase P of Hormoconis resinae. Current Genetics, 1994, 26, 422-429.
80. Jeenes, D. J., Marczinke, B., MacKenzie, D. A. and Archer, D, B., A truncated glucoamylase gene fusion for heterologous protein secretion from Aspergillus nidulans. FEMS Microbiology Letters, 1993, 107, 267-271.
81. Morita, S., Kuriyama, M., Nakatsu, M., Suzuki, M. and Kitano, K., Secretion of active human lysozyme by Acremonium chrysogenum using a Fusarium alkaline protease promoter system. Journal of Biotechnology, 1995, 42, 1-8.
82. Stewart, P., Whitwam, R. E., Kersten, P. J., Cullen, D. and Tien, M., Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. Applied and Environmental Microbiology, 1996, 62, 860-864.
83. 2 fusion protein is secreted and processed to yield mature enzyme. Gene, 1992, 122, 155-161.
84. Hessing, H. J. G. M., van Rotterdam, C., Verbakel, J. A. M., Roza, M., Maat, J., Gorcom, R. F. M. and van den Hondel, C. A. M. J. J., Isolation and characterization of a 1,4-β-endoxylanase gene at A. awamori. Current Genetics, 1994, 26, 228-232.