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Volumn 9, Issue 15, 1999, Pages 2249-2254

Photonuclease activity of Taylor's blue

Author keywords

[No Author keywords available]

Indexed keywords

METHYLENE BLUE; NUCLEIC ACID;

EID: 0033516956     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(99)00362-5     Document Type: Article
Times cited : (15)

References (31)
  • 19
    • 0009652583 scopus 로고    scopus 로고
    • note
    • 3OD): δ 19.01, 41.30, 105.63, 119.08, 148.91, 150.64, 154.22, 155.45 ppm.
  • 23
    • 0009588864 scopus 로고    scopus 로고
    • note
    • + is essentially excited with the 578-nm mercury line.
  • 24
    • 0009631674 scopus 로고    scopus 로고
    • note
    • Each sample was treated with 1/6th volume of bromophenol blue tracking dye (0.25% dye and 40% sucrose in water) and 10 μL was loaded on a 1% neutral agarose gel containing 2 μL of EtBr (10 mg/mL). The gel was run in 1X TBE buffer containing 3 μL of EtBr (10 mg/mL) at 76 V for ca 4 h. The gel photograph was recorded on a Polaroid high-speed film type 57 using a Polaroid MP-3 Land Camera.
  • 25
    • 0031578703 scopus 로고    scopus 로고
    • + in the absence of DNA was observed, as was evidenced from both UV-Vis and HPLC analyses. It has been shown that DNA protects intercalating phenothiazine dyes from photodegradation by quenching the dye singlet excited state, see reference 11a and Mohammad, T.; Morrison, H. J. Chromatogr. B 1997, 704, 265.
    • (1997) J. Chromatogr. B , vol.704 , pp. 265
    • Mohammad, T.1    Morrison, H.2
  • 27
    • 0024633438 scopus 로고
    • A Kodak Digital Science DC40 Camera in combination with the 1D Analysis Software Version 1.6 was used for the quantification of the DNA bands. This equipment was available in the laboratory of Dr. D. Bergstrom with the assistance of Maneesh Pingle. The net intensity of the digitized bands was used after subtracting the background fluorescence. A correction factor of 1.66 was employed to normalize the data for RFII that gives higher EtBr fluorescence when bound to DNA, see (a) Ciulla, T. A.; van Camp, J. R; Rosenfeld, E.; Kochevar, I. E. Photochem. Photobiol. 1989 49, 293.
    • (1989) Photochem. Photobiol. , vol.49 , pp. 293
    • Ciulla, T.A.1    Van Camp, J.R.2    Rosenfeld, E.3    Kochevar, I.E.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.