Construction and characterization of a one-plasmid system for the controlled expression of genes in mammalian cells by tetracycline

Gene

Volumn 184, Issue 1, 1997, Pages 115-120

  O'Brien, Kathryn ^a   Otto, Keith ^a   Rao, R Nagaraja ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

Cloning; Hygromycin; p53; Raf; Stable transfection; tetR; Transformed phenotype; VP16

Indexed keywords

PROTEIN P53; RAF PROTEIN; TETRACYCLINE;

ANIMAL CELL; ARTICLE; CELL TRANSFORMATION; CONTROLLED STUDY; EXPRESSION VECTOR; GENE CONSTRUCT; GENE EXPRESSION REGULATION; GENE REPRESSION; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; KIDNEY EPITHELIUM; MAMMAL; MOUSE; NONHUMAN; OSTEOSARCOMA CELL; PLASMID; PRIORITY JOURNAL;

ANIMALS; BLOTTING, WESTERN; DNA PRIMERS; GENE EXPRESSION REGULATION; HUMANS; KIDNEY; PLASMIDS; POLYMERASE CHAIN REACTION; PROTEIN-SERINE-THREONINE KINASES; PROTO-ONCOGENE PROTEINS; PROTO-ONCOGENE PROTEINS C-RAF; RATS; TETRACYCLINE; TRANSFECTION; TRANSFORMATION, GENETIC; TUMOR CELLS, CULTURED; TUMOR SUPPRESSOR PROTEIN P53;

EID: 0031036393     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(96)00583-5     Document Type: Article

References (25)

1. 1 cell cycle checkpoints and mediates reversible growth arrest in human fibroblasts. Proc. Natl. Acad. Sci. USA 92, 8493-8497.
2. 1 to S phase. EMBO J. 14, 5618-5625.
3. Citron, B, Falck-Pedersen, E., Saldott-Georgieff, M. and Darnell J.E. Jr. (1984) Transcription termination occurs within a 1000 base pair region downstream from the poly (A) site of mouse beta globin (major) gene. Nucleic Acids Res. 12, 8723-8731.
4. Cuadrado, A., Bruder, J.T., Heidaran, M.A., App, H., Rapp, U.R. and Aaronson, S.A. (1993) H-ras and raf-1 cooperate in transformation of NIH3T3 fibroblasts. Oncogene 8, 2443-2448.
5. Deuschle, U., Meyer, W.K.-H. and Thiesen H.-J. (1995) Tetracycline-reversible silencing of eukaryotic promoters. Mol. Cell Biol. 15, 1907-1914.
6. Efrat, S., Fusco-DeMane, D., Lemberg, H., Emran, O.A. and Wang, X. (1995) Conditional transformation of a pancreatic B-cell line derived from transgenic mice expressing a teracycline-regulated oncogene. Proc. Natl. Acad. Sci. USA 92, 3576-3580.
7. Furth, P.A., St. Onge, L., Böger, H., Gruss, P., Gossen, M., Kistner, A., Bujard, H. and Hennighausen, L. (1994) Temporal control of gene expression in transgenic mice by a tetracycline-responsive promoter. Proc. Natl. Acad. Sci. USA 91, 9302-9306.
8. Gait, M.J. and Karn, J. (1993) RNA recognition by the human immunodeficiency virus tat and rev proteins. Trends Biochem. Sci. 18, 255-259.
9. 1-phase control. Biol. Chem. Hoppe-Seyler 376, 441-446.
10. Goodwin, E.C. and Rottman, F.M. (1992) The 3′-flanking sequence of the bovine growth hormone gene contains novel elements required for efficient and accurate polyadenylation. J. Biol. Chem. 267, 16330-16334.
11. Gossen, M. and Bujard, H. (1992) Tight control of gene expression in mammalian cells by tetracycline-responsive promoters. Proc. Natl. Acad. Sci. USA 89, 5547-5551.
12. Gossen, M., Freundlieb, S., Bender, G., Müller, G., Hillen, W. and Bujard, H. (1995) Transcriptional activation by tetracyclines in mammalian cells. Science 268, 1766-1769.
13. Hennighausen, L., Wall, R.J., Tillmann, U., Li, M. and Furth, P.A. (1995) Conditional gene expression in secretory tissues and skin of transgenic mice using the MMTV-LTR and the tetracycline responsive system, J. Cell. Biochem. 59, 1-10.
14. Howe, J.R., Skyrabin, B.V., Belcher, S.M., Zerillo, C.A. and Schmauss, C. (1995) The responsiveness of a tetracycline-sensitive expression system differs in different cell lines. J. Biol. Chem. 270, 14168-14174.
15. Jaffrey, S.R., Haile, D.J., Klausner, R.D. and Harford, J.B. (1993) The interaction between the iron-responsive element binding protein and its cognate RNA is highly dependent upon both RNA sequence and structure. Nucleic Acids Res. 21, 4627-4631.
16. Kolch, W., Heidecker, G., Lloyd, P. and Rapp, U.R. (1991) Raf-1 protein kinase is required for growth of induced NIH/3T3 cells. Nature 349, 426-428.
17. Masuda, H., Miller, C., Koeffler, H.P., Battifora, H. and Cline, M.J. (1987) Rearrangement of the p53 gene in human osteogenic sarcomas. Proc. Natl. Acad. Sci. USA 84, 7716-7719.
18. Paulus, W., Baur, I., Boyce, F.M., Breakefield, X.O. and Reeves, S. (1996) Self-contained and tetracycline-regulated retroviral vector system for gene delivery to mammalian cells. J. Virol. 70, 62-67.
19. 1 to S transition. Mol. Cell. Biol. 15, 3463-3469.
20. kip1. Mol. Cell. Biol. 15, 4347-4352.
21. Reynisoóttir, I., Polyak, K., Iovarone, A. and Massagúe, J. (1995) Kip/Cip and Ink4 cdk inhibitors cooperate to induce cell cycle arrest in response to TGF-β. Genes Dev. 9, 1831-1845.
22. Schultze, N., Burki, Y., Lang Y., Certa, U. and Bluethmann, H. (1996) Efficient control of gene expression by single step integration of the tetracycline system in transgenic mice. Nature Biotechnol. 14, 499-503.
23. Shockett, P., Difilippantonio, M., Hellman N. and Schatz, D.G. (1995) A modified tetracycline-regulated system provides autoregulatory, inducible gene expression in cultured cells and transgenic mice. Proc. Natl. Acad. Sci. USA 92, 6522-6526.
24. Southern, P.J. and Berg, P. (1982) Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Genet. 327-341.
25. 1 progression through tetracycline-regulated expression of human cyclin E. Oncogene 9, 995-997.