Isolation and characterization of ethanol-tolerant mutants of Escherichia coli KO11 for fuel ethanol production

Journal of Industrial Microbiology and Biotechnology

Volumn 20, Issue 2, 1998, Pages 132-138

  Yomano, L P ^a   York, S W ^a   Ingram, L O ^a  

^a UNIVERSITY OF FLORIDA   (United States)

Author keywords

Biomass; Ethanol; Ethanol tolerance; Fermentation; Lignocellulose; Xylose

Indexed keywords

XYLOSE;

ALCOHOL TOLERANCE; ARTICLE; CARBOHYDRATE METABOLISM; ESCHERICHIA COLI; FERMENTATION; MICROBIOLOGY; MUTANT; SACCHAROMYCES; TECHNIQUE; ZYMOMONAS MOBILIS;

ESCHERICHIA COLI; ETHANOL; FERMENTATION; GENETIC ENGINEERING; GLUCOSE; MUTATION; TIME FACTORS; XYLOSE;

ESCHERICHIA COLI; NEGIBACTERIA; SACCHAROMYCES; ZYMOMONAS MOBILIS;

EID: 0031923093     PISSN: 13675435     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.jim.2900496     Document Type: Article

References (39)

1. Asghari A, RJ Bothast, JB Doran and LO Ingram. 1996. Ethanol production from hemicellulose hydrolysates of agricultural residues using genetically engineered Escherichia coli KO11. J Ind Microbiol 16: 42-47.
2. Atlas RM and LC Parks (eds). 1993. Handbook of Microbiological Media. CRC Press, Boca Raton, FL.
3. Barbosa M de FS, LP Yomano and LO Ingram. 1994. Cloning, sequencing, and expression of the stress genes from the ethanol-producing bacterium Zymomonas mobilis: the groESL operon. Gene 148: 51-57.
4. Beall DS and LO Ingram. 1993. Genetic engineering of soft-rot bacteria for ethanol production from lignocellulose. J Ind Microbiol 11: 151-155.
5. Beall DS, K Ohta and LO Ingram. 1991. Parametric studies of ethanol production from xylose and other sugars by recombinant Escherichia coli. Biotechnol Bioeng 38: 296-303.
6. Clark DP and JP Beard. 1979. Altered phospholipid composition in mutants of Escherichia coli sensitive or resistant to organic solvents. J Gen Microbiol 113: 267-274.
7. Dombek KM and LO Ingram. 1986. Magnesium limitation and its role in the apparent toxicity of ethanol during yeast fermentation. Appl Environ Microbiol 52: 975-981.
8. Dombek KM and LO Ingram. 1984. Effects of ethanol on the Escherichia coli plasma membrane. J Bacteriol 157: 233-239.
9. Fried VA and A Novick. 1973. Organic solvents as probes for the structure and function of the bacterial membrane: effects of ethanol on the wild type and an ethanol-resistant mutant of Escherichia coli K-12. J Bacteriol 114: 239-248.
10. Hahn-Hagerdal B. 1996. Ethanolic fermentation of lignocellulose hydrolysates: a minireview. Appl Biochem Biotechnol 57/58: 195-199.
11. Hahn-Hagerdal B, H Jeppsson, L Olsson and A Mohagheghi. 1994. An interlaboratory comparison of the performance of ethanol-producing micro-organisms in a xylose-rich acid hydrolysate. Appl Microbiol Biotechnol 41: 62-72.
12. Heipieper HJ and JAM de Bont. 1994. Adaptation of Pseudomonas putida S12 to ethanol and toluene at the level of fatty acid composition of membranes. Appl Environ Microbiol 60: 4440-4444.
13. Ingram LO. 1990. Ethanol tolerance in bacteria. Crit Rev Biotechnol 9: 305-319.
14. Ingram LO. 1986. Microbial tolerance to alcohols: role of the cell membrane. Trends Biotechnol 4: 40-44.
15. Ingram LO. 1982. On the regulation of fatty acid composition in Escherichia coli: a proposed common mechanism for changes induced by ethanol, chaotropic agents and a reduction in growth temperature. J Bacteriol 149: 166-172.
16. Ingram LO. 1981. Mechanism of lysis of Escherichia coli by ethanol and other chaotropic agents. J Bacteriol 146: 331-336.
17. Ingram LO. 1977. Preferential inhibition of phosphalidyl ethanolamine synthesis in E. coli by alcohols. Can J Bacteriol 23: 779-789.
18. Ingram LO, F Alterthum and T Conway. 1991. Ethanol production by Escherichia coli strains co-expressing Zymomonas pdc and adh genes. US Patent No. 5 000 000.
19. Ingram LO and MDF Barbosa-Alleyne. 1996. Ethanol production by gram-positive microbes. US Patent No. 5 482 846.
20. Ingram LO, DS Beall, GFH Burchhardt, WV Guimaraes, K Ohta, BE Wood, KT Shanmugam, DE Fowler and A Ben-Bassat. 1995. Ethanol production by recombinant hosts. US Patent No. 5 424 202.
21. Ingram LO and TM Buttke. 1984. Effects of alcohols on micro-organisms. Adv Microb Physiol 25: 253-300.
22. Ingram LO and T Conway. 1988. Expression of different levels of ethanologenic enzymes of Zymomonas mobilis in recombinant strains of Escherichia coli. Appl Environ Microbiol 54: 397-404.
23. Ingram LO, T Conway, DP Clark, GW Sewell and JF Preston. 1987. Genetic engineering of ethanol production in Escherichia coli. Appl Environ Microbiol 53: 2420-2425.
24. Ingram LO and KM Dombek. 1989. Effects of ethanol on Escherichia coli. In: Alcohol Toxicity in Yeasts and Bacteria (van Uden N, ed), pp 227-238, CRC Press/Boca Raton, FL.
25. Ingram LO, X Lai, M Moniruzzaman, BE Wood and SW York. 1997. Fuel ethanol production from lignocellulose using genetically engineered bacteria. In: Fuels and Chemicals from Biomass, ACS Symposium Series 666 (Saha BC and J Woodward, eds), pp 57-73, American Chemical Society, Washington, DC.
26. Ingram LO, YA Osman, T Conway and KM Dombek. 1989. Zymomonas mobilis: effects of ethanol on fermentation. In: Alcohol Toxicity in Yeasts and Bacteria (van Uden N, ed), pp 257-270. CRC Press, Boca Raton, FL.
27. Katzen R and DE Fowler. 1994. Ethanol from lignocellulosic wastes with utilization of recombinant bacteria. Appl Biochem Biotechnol 45/46: 697-707.
28. Krishnan MS, Y Xia, NWY Ho and GT Tsao. 1997. Fuel ethanol production from lignocellulosic sugars: studies using a genetically engineered Saccharomyces yeast. In: Fuels and Chemicals from Biomass, ACS Symposium Series 666 (Saha BC and J Woodward, eds), pp 74-92, American Chemical Society, Washington, DC.
29. Lindsay SE, RJ Bothast and LO Ingram. 1995. Improved strains of recombinant Escherichia coli for ethanol production from sugar mixtures. Appl Microbiol Biotechnol 43: 70-75.
30. Missiakas D and S Raina. 1997. Protein folding in the bacterial periplasm. J Bacteriol 179: 2465-2471.
31. Ohta K, DS Beall, JP Mejia, KT Shanmugam and LO Ingram. 1991. Genetic improvement of Escherichia coli for ethanol production: chromosomal integration of Zymomomas mobilis genes encoding pyruvate decarboxylase and alcohol dehydrogenase II. Appl Environ Microbiol 57: 893-900.
32. Peaff G. 1997. Ethanol in fuel debated again. Chem Eng News 4: 20-22.
33. Picataggio SK, M Zhang, CK Eddy, KA Deanda and M Finkelstein. 1996. Recombinant Zymomonas for pentose fermentation. US Patent No. 5514583.
34. Pinkart HC and DC White. 1997. Phospholipid biosynthesis and solvent tolerance in Pseudomonas putida strains. J Bacteriol 179: 4219-4226.
35. Sikkema J, JAM de Bont and B Poolman. 1995. Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59: 201-222.
36. Thomas KC, SH Hynes and WM Ingledew. 1996. Effect of nitrogen limitation on synthesis of enzymes in Saccharomyces cerevisiae during fermentation of high concentrations of carbohydrates. Biotechnol Lett 18: 1165-1168.
37. Walfridsson M, X Bao, M Anderlund, G Lilius, L Bulow and B Hahn-Hagerdal. 1996. Ethanologenic fermentation of xylose with Saccharomyces cerevisiae harboring the Thermus thermophilus xylA gene, which expresses an active xylose isomerase. Appl Environ Microbiol 62: 4648-4651.
38. Wood BE, HC Aldrich and LO Ingram. 1997. Ultrasound stimulates ethanol production during the simultaneous saccharification and fermentation of mixed waste office paper. Biotechnol Prog 13: 232-237.
39. Zhang M, C Eddy, K Deanda, M Finkelstein and S Picalaggio. 1995. Metabolic engineering of a pentose pathway in ethanologenic Zymomonas mobilis. Science 267: 240-243.