Expression and characterization of E. coli-produced soluble, functional human dihydroorotate dehydrogenase: A potential target for immunosuppression

Journal of Molecular Microbiology and Biotechnology

Volumn 1, Issue 1, 1999, Pages 183-188

  Neidhardt, Edie A ^a,c   Punreddy, Sandhya R ^a,d   McLean, Jeremy E ^b   Hedstrom, Lizbeth ^b   Grossman, Trudy H ^a,e  

^a Procept Inc   (United States)

^b BRANDEIS UNIVERSITY   (United States)

^c PFIZER INC   (United States)

^d MILLENNIUM PHARMACEUTICALS INC   (United States)

^e ARIAD PHARMACEUTICALS INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

4,5 DIHYDROOROTIC ACID; 4,5-DIHYDROOROTIC ACID; DIHYDROOROTATE DEHYDROGENASE; DRUG DERIVATIVE; HISTIDINE; HYBRID PROTEIN; OROTIC ACID; OXIDOREDUCTASE; UBIQUINONE;

ANIMAL; ARTICLE; CATTLE; CELL FRACTIONATION; ESCHERICHIA COLI; GENETICS; HUMAN; IMMUNOSUPPRESSIVE TREATMENT; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR CLONING; PHYSIOLOGY; SOLUBILITY;

ANIMALS; CATTLE; CLONING, MOLECULAR; ESCHERICHIA COLI; HISTIDINE; HUMANS; IMMUNOSUPPRESSION; KINETICS; OROTIC ACID; OXIDOREDUCTASES; OXIDOREDUCTASES ACTING ON CH-CH GROUP DONORS; RECOMBINANT FUSION PROTEINS; SOLUBILITY; SUBCELLULAR FRACTIONS; UBIQUINONE;

EID: 0033170743     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Bjornberg, O., Rowland, P., Larsen, S., and Jensen, K.F. 1997. Active site of dihydroorotate dehydrogenase A from Lactococcus lactis investigated by chemical modification and mutagenesis. Biochem. 36: 16197-16205.
2. Chen, S.F., Perrella, F.W., Behrens, D.L., and Papp, L.M. 1992. Inhibition of dihydroorotate dehydrogenase activity by brequinar sodium. Cancer Res. 52: 3521-3527.
3. Chen, J.J., and Jones, M.E. 1976. The cellular location of dihydroorotate dehydrogenase: relation to de novo biosynthesis of pyrimidines. Arch. Biochem. Biophys. 176: 82-90.
4. Cleland, W.W. 1977. Determining the chemical mechanisms of enzyme-catalyzed reactions by kinetic studies. Adv. Enzymol. 45: 273-387
5. Copeland, R.A., Davis, J.P., Dowling, R.L., Lombardo, D., Murphy, K.B., and Patterson, T.A. 1995. Recombinant human dihydroorotate dehydrogenase: Expression, purification, and characterization of a catalytically functional truncated enzyme. Arch. Biochem. Biophys. 323: 79-86.
6. Davis, J.P., Cain, G.A., Pitts, W.J., Magolda, R.L., and Copeland, R.A. 1996. The immunosuppressive metabolite of leflunomide is a potent inhibitor of human dihydroorotate dehydrogenase. Biochem. 35: 1270-1273.
7. Faeder, E.J., and Siegel, L.M. 1973. A rapid micromethod for determination of FMN and FAD in mixtures. Anal. Biochem. 53: 332-336
8. Forman, H.J., and Kennedy J. 1978. Mammalian dihydroorotate dehydrogenase: Physical and catalytic properties of the primary enzyme. Arch. Biochem. Biophys. 191: 23-31.
9. Gero, A.M., and O'Sullivan, W.J. 1985. Human spleen dihydroorotate dehydrogenase: properties and partial purification. Biochem. Med. 34: 70-82.
10. Grossman, T.H., Kawasaki, E.S., Punreddy, S. R., and Osburne, M.S. 1998. Spontaneous cAMP-dependent derepression of gene expression in stationary phase plays a role in recombinant expression instability. Gene. 209: 95-103.
11. Hines, V., and Johnston, M. 1989. Analysis of the kinetic mechanism of the bovine liver mitochondrial dihydroorotate dehydrogenase. Biochem. 28: 1222-1226.
12. Hines, V., Keys, L.D., and Johnston, M. 1986. Purification and properties of the bovine liver mitochondrial dihydroorotate dehydrogenase. J. Biol. Chem. 261: 11386-11392.
13. Jones, M.E. 1980. Pyrimidine nucleotide biosynthesis in animals: Genes, enzymes, and regulation of UMP biosynthesis. Ann. Rev. Biochem. 49: 253-79.
14. Kennedy, J. 1973. Distribution, subcellular localization, and product inhibition of dihydroorotate oxidation in the rat. Arch. Biochem. Biophys. 157: 369-373.
15. Knecht, W., Altekruse, D., Rotgeri, A., Gonski, S., and Loffler, M. 1997. Rat dihydroorotate dehydrogenase: Isolation of the recombinant enzyme from mitochondria of insect cells. Protein Exp. Purif. 10: 89-99.
16. Knecht, W., Bergjohann, U., Gonski, S., Kirschbaum, B., and Loffler, M. 1996. Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme. Eur. J. Biochem. 240: 292-301.
17. Larsen, J.N., and Jensen, K. F. 1985. Nucleotide sequence of the pyrD gene of Escherichia coli and characterization of the flavoprotein dihydroorotate dehydrogenase. Eur. J. Biochem. 151: 59-65.
18. Miller, J.H. 1992. A short course in bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
19. Miller, R.W. 1975. A high molecular weight dihydro-orotate dehydrogenase of Neurospora crassa. Purification and properties of the enzyme. Can. J. Biochem. 53: 1288-1300.
20. Miller, R.W., Kerr, C.T., and Curry, J.R. 1968. Mammalian dihydroorotate-ubiquinone reductase compex. Can. J. Biochem. 46: 1099-1106.
21. Minet, M., Dufour, M.E., and Lacroute, F. 1992. Cloning and sequencing of a human cDNA coding for dihydroorotate dehydrogenase by complementation of the corresponding yeast mutant. Gene. 121: 393-396.
22. Rotgeri, A., and Loffler, M. 1995. Molecular cloning and sequence analyses of rat liver dihydroorotate dehydrogenase. In: Purine and Pyrimidine Metabolism in Man VIII. A. Sahota and M. Taylor, eds. Plenum Press, New York. p. 693-697.
23. Rowland, P., Nielsen, F.S., Jensen, K.F., and Larsen, S. 1997. The crystal structure of the flavin containing enzyme dihydroorotate dehydrogenase A from Lactococcus lactis. Structure. 5: 239-252.
24. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M. D., Fujimoto, E. K., Goeke, N. M., Olson, B. J., and Klenk, D. C. 1985. Measurement of Protein Using Bicinchoninic Acid. Anal. Biochem. 150: 76-853.
25. Whitby, L.G. 1953. A new method for preparing flavin-adenine dinucleotide. Biochem. J. 54: 437-442.