Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme

European Journal of Biochemistry

Volumn 240, Issue 1, 1996, Pages 292-301

  Knecht, Wolfgang ^a  

^a PHILIPPS UNIVERSITY MARBURG   (Germany)

Author keywords

Baculovirus; Dehydrogenase; Kinetics; Mitochondria; Recombinant

Indexed keywords

DIHYDROOROTATE DEHYDROGENASE; LEFLUNOMIDE; TERIFLUNOMIDE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ENZYME ASSAY; ENZYME HISTOCHEMISTRY; ENZYME INHIBITION; ENZYME KINETICS; EXPRESSION VECTOR; HUMAN; IMMUNOBLOTTING; LIVER; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0029793899     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0292h.x     Document Type: Article

References (3)

1. Jones, M. E. (1980) Pyrimidine nucleotide biosynthesis in animals: genes, enzymes and regulation of UMP biosynthesis, Annu. Rev. Biochem. 49, 253-279.
2. Löffler, M. (1980) On the role of dihydroorotate dehydrogenase in growth cessation of Ehrlich ascites tumor cells cultured under oxygen deficiency, Eur. J. Biochem. 107, 207-215.
3. Amellem, O., Löffler, M. & Pettersen, E. O. (1994) Regulation of cell proliferation under extreme and moderate hypoxia: the role of pyrimidine (deoxy)nucleotides, Br. J. Cancer 70, 857-866.