Determination of functional domains of the human transcription factor PAX8 responsible for its nuclear localization and transactivating potential

European Journal of Biochemistry

Volumn 247, Issue 3, 1997, Pages 860-869

  Poleev, Andrej ^a   Okladnova, Olga ^b   Musti, Anna Maria ^c   Schneider, Susanne ^d   Royer Pokora, Brigitta ^d   Plachov, Dimitrij ^e,f  

^a UNIVERSITY HOSPITAL ESSEN   (Germany)

^b UNIVERSITY OF WÜRZBURG   (Germany)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d Inst for Hum Genet and Anthropol   (Germany)

^e GeneCraft   (Germany)

^f GeneCratt   (Germany)

Author keywords

cAMP dependent protein kinase; Nuclear localization site; Pax gene; PAX8; Transcription activation and repression domains

Indexed keywords

CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; THYROGLOBULIN; THYROID PEROXIDASE; THYROTROPIN; TRANSCRIPTION FACTOR;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL CULTURE; CELL LINE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONTROLLED STUDY; EXON; GENE ACTIVATION; GENE EXPRESSION; GENETIC ANALYSIS; IN VITRO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; RAT; SIGNAL TRANSDUCTION; THYROID CELL;

ANIMALIA;

EID: 0030746904     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00860.x     Document Type: Article

References (71)

1. Adams, B., Dörfler, P., Aguzzi, A., Kozmik, Z., Urbanek, P., Maurer-Fogy, I. & Busslinger, M. (1992) Pax-5 encodes the transcription factor BSAP and is expressed in B lymphocytes, the developing CNS, and adult testis, Genes & Dev. 6, 1589-1607.
2. Ambesi-Impiombato, F. S., Parks, L. A. M. & Coon, H. G. (1980) Culture of hormone-dependent functional epithelial cells from rat thyroids, Proc. Natl Acad. Sci. USA 6, 3455-3459.
3. Avvedimento, V. E., Musti, A. M., Ueffing, M., Obici, S., Gallo, A., Sanchez, M., De Brasi, D. & Gottesman, M. E. (1991) Reversible activation of a thyroid specific trims-acting factor by Ras, Genes & Dev. 5, 22-28.
4. Barr, F. G., Galili, N., Holick J., Biegel, J. A., Rovera, G. & Emanuel, B. S. (1993) Rearragement of the PAX3 paired box gene in the paediatric solid tumour alveolar rhabdomyosarcoma, Nat. Genet. 3, 113-117.
5. Bopp, D., Burri, M., Baumgartner, S., Frigerio, G. & Noll, M. (1989) Conservation of a large protein domain in the segmentation gene paired and functionally related genes of Drosophila, Cell 47, 1033-1040.
6. Bull, P., Morley, K. L., Hoekstra, M. F., Hunter, T. & Verma, I. M. (1990) The mouse c-rel protein has an N-terminal regulatory domain and C-terminal transcriplional transactivation domain, Mol. Cell. Biol. 10, 5473-5485.
7. Carriere, C., Plaza, S., Martin P., Quatannens, B., Bailly, M., Stehalin, D. & Saule, S. (1993) Characterization of quail Pax-6 (Pax-QNR) proteins expressed in the neuroretina, Mol. Cell. Biol. 13, 7257-7266.
8. Chalepakis, G., Fritsch, R., Fickenscher, H., Deutsch, U., Goulding, M. & Gruss, P. (1991) The molecular basis of the undulated/Pax-1 mutation, Cell 66, 873-884.
9. Chalepakis, G., Jones, F. S., Edelman, G. M. & Gruss, P (1994a) Pax-3 contains domains for transcription activation and transcription inhibition, Proc. Natl Acad. Sci. USA 91, 12745-12749.
10. Chalepakis, G., Wijnholds, J. & Gruss, P. (1994b) Pax-3-DNA interaction: flexibility in the DNA binding and induction of DNA conformational changes by paired domains, Nucleic Acids Res. 22, 3131-3137.
11. Chelsky, D., Ralph, R. & Jonak, G. (1989) Sequence requirements for synthetic peptide-mediated translocation to the nucleus, Mol. Cell. Biol. 9, 2487-2492.
12. Civitareale, D., Lonigro, R., Sinclair, A. J. & Di Lauro, R. (1989) A thyroid-specific nuclear protein essential for tissue-specific expression of the thyroglobulin promoter, EMBO J. 8, 2537-2542.
13. Colletta, G., Pinto, A., Di Fiore, P. P., Fusco, A., Ferrentino, M., Avvedimento, V. E., Tsuchida, N. & Vecchio, G. (1983) Dissociation hetween transformed and differentiated phenotype in rat thyroid epithelial cells after transformation with a temperature-sensitive mutant of the Kirsten murine sarcoma virus, Mol. Cell. Biol. 3, 2099-2109.
14. Czerny, T., Schaffner, G. & Busslinger, M. (1993) DNA sequence recognition by Pax proteins: bipartite structure of the paired domain and its binding site, Genes & Dev. 7, 2048-2061.
15. Davis, R. J., D'Cruz, C. M., Lovell, M. A., Biegel, J. A. & Barr, F. G. (1994) Fusion of PAX7 to FKHR by the variant 1(1;13) translocation in alveolar rhabdomyosarcoma, Cancer Res. 54, 2869-2872.
16. Dehbi, M. & Pelletier, J. (1996) PAX8-mediated activation of the wt1 tumor suppressor gene, EMBO J. 15, 4297-4306.
17. Dingwall, C. & Laskey, R. (1991) Nuclear targeting sequences a consensus? Trends Biochem. Sci. 16, 478-481.
18. Dörfler, P. & Busslinger, M. (1996) C-terminal activating and inhibitory domains determine the transactivation potential of BSAP (Pax-5), Pax-2 and Pax-8, EMBO J. 15, 1971-1982.
19. Eccles, M. R., Yun, K., Reeves, A. E. & Fidler, A. E. (1995) Comparative in situ hybridization analysis of PAX2, PAX8, and WT1 gene transcription in human fetal kidney and Wilms' tumors, Am. J. Pathol. 146, 40-46.
20. Epstein, J., Cai, J., Glaser, T., Jepeal, L. & Maas, R. (1994) Identification of a paired domain recignition sequence and evidence for DNA-dependent conformational changes, J. Biol. Chem. 269, 8355-8361.
21. Fleischmann, R. D., Jeng, C. & Gottesman, M. M. (1992) Ablation of stimulation of a cAMP-responsive promoter in CHO cell lines defective in their cAMP-depcedent protein kinase system, Som. Cell Mol. Genet. 18, 103-111.
22. Foulkes, N. S., Borrelli, E. & Sassone-Corsi, P. (1991) CREM gene: use of alternative DNA-binding domains generates multiple antagonists of cAMP-induced transcription, Cell 64, 739-749.
23. Francis-Lang, H., Zannini, M., De Felice, M., Berlingieri, M. S., Fusco, A. & Di Lauro, R. (1992) Multiple mechanisms of interference between transformation and differentiation in thyroid cells, Mol. Cell. Biol. 12, 5793-5800.
24. Gallo, A., Benusiglio, R., Bonapace, I. M., Feliciello, A., Cassano, S., Garbi, C., Musti, A. M., Gottesman, M. E. & Avvedimento, E. V. (1992) v-ras and protein kinase C dedifferentiate thyroid cells by downreguluting nuclear cyclic AMP-dependent protein kinase A, Genes & Dev. 6, 1621-1630.
25. Glaser, T., Walton, D. S., Cai, J., Epstein, J. A., Japeal, L. & Maas, R. L. (1995) PAX6 gene mutations in aniridia, in Molecular genetics of ocular disease, pp. 51-82, Wiley, New York.
26. Gonzalez, G. A., Yamamoto, K. K., Fischer, W. H., Karr, D., Menzel, P., Biggs, W. III, Vale, W. W. & Montminy, M. R. (1989) A cluster of phosphorylation sites on the cyclic AMP-regulated nuclear factor CREB predicted by its sequence, Nature 337, 749-752.
27. Hai, T., Liu, F., Coukos, W. J. & Green, M. R. (1989) Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers, Genes & Dev. 3, 2083-2090.
28. Hoeffler, J. P., Meyer, T. E., Yun, Y., Jameson, J. L. & Habener, J. F. (1988) Cyclic AMP-responsive DNA-binding protein: structure based on a cloned placental cDNA, Science 243, 1430-1433.
29. Imagawa, M., Chiu, R. & Karin, M. (1987) Transcription factor AP-2 mediates induction by two different signal-transduction pathways: protein kinase C and cAMP, Cell 51, 251-260.
30. Jones, K. A., Yamamoto, K. R. & Tjian, R. (1985) Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro, Cell 42, 559-572.
31. Kalderon, D., Roberts, B. L., Richardson, W. D. & Smith, A. (1984) A short amino acid sequence able to specify nuclear location, Cell 39, 499-509.
32. Kapiloff, M. S., Farkash, Y., Wegner, M. & Rosenfeld, M. G. (1991) Variable effects of phosphorylation of Pit-1 dictated by the DNA response elements, Science 253, 786-789.
33. Kappler, J. W., Herman, A., Clements, J. & Marrack, P. (1992) Mutations defining functional regions of the superantigen staphylococcal enterotoxin B, J. Exp. Med. 175, 387-396.
34. Kennelly, P. J. & Krebs, E. G. (1991) Consensus sequences as substrate specificity determinants for protein kinases and phosphatases, J. Biol. Chem. 266. 15555-15558.
35. Kowenz-Leutz, E., Twamley, G., Ansieau, S. & Leutz, A. (1994) Novel mechanism of C/EBPb (NF-M) transcriptional control: activation through derepression, Genes & Dev. 8, 2781-2791.
36. Kozmik, Z., Kurzbauer, R., Dorfler, P. & Busslinger, M. (1993) Alternative splicing of Pax-8 gene transcripts is developmentally regulated and generates isoforms with different transactivation properties, Mol. Cell. Biol. 13, 6024-6035.
37. Lee, C. Q., Yun, Y., Hoeffler, J. P. & Habener, J. F. (1990) Cyclic-AMP-responsive transcriptional activation of CREB-327 involves interdependent phosphorylated sudomains, EMBO J. 9, 4455-4465.
38. Lehming, N., Thanos, D., Brickman, J. M., Ma, J., Maniatis, T. & Ptashne, M. (1994) An HMG-like protein that can switch a transcriptional activator to a repressor, Nature 371, 175-179.
39. Leschner, M. S. & Dressler, G. R. (1996) Mapping of Pax-2 transcription activation domain, J. Biol. Chem. 271, 21088-21093.
40. Matheny, C., Day, M. L. & Milbrandt, J. (1994) The nuclear localization signal of NGFI-A is located within the zinc finger binding domain, J. Biol. Chem. 269, 8176-8181.
41. Mellon, P. L., Clegg, C. H., Correll, L. A. & McKnight, G. S. (1989) Regulation of transcription by cyclic AMP-dependent protein kinase, Proc. Natl Acad Sci. USA 86, 4887-4891.
42. Montminy, M. R. Gonzalez, G. A. & Yamamoto, K. (1990) Regulation of cAMP-inducable genes by CREB, Trends Neurosci. 13, 184-188.
43. Nichols, M., Weih F., Schmid, W., DeVack, C., Kownz-Leutz, E., Luckow, B., Boshart, M. & Schütz, G. (1992) Phosphorylation of CREB affects its binding to high and low affinity sites: implications for cAMP induced gene transcription, EMBO J. 11, 3337-3346.
44. Nigg, E. A., Hilz, H., Eppenberger, H. M. & Dutly, F. (1985) Rapid and reversible translocation of the catalytic subunit of cAMP-dependent protein kinase type II from the Golgi complex to the nucleus, EMBO J. 4, 2801-2806.
45. Okladnova, O., Poleev, A., Fantes, J., Lee, M. & Plachov, D. (1997) Genomic organization of the murine Pax8 gene and characterization of its basal promoter, Genomics, in the press.
46. Peers, B., Monget, P., Nalda, M. A., Voz, M. L., Berwaer, M., Belayew, A. & Martial, J. A. (1991) Transcriptional induction of the human prolactin gene by cAMP requires two cis-acting elements and at least the pituitary-specific factor Pit-1, J. Biol. Chem. 266, 18127-18134.
47. Plachov, D., Chowdhury, K., Walter, C., Simon, D., Guenet, J.-L. & Gruss, P. (1990) Pax8, a murine paired box gene expressed in the developing excretory system and thyroid gland, Development 110, 643-651.
48. Plaza, S., Dozier, C. & Saule, S. (1993) Quail PAX-6 (PAX-QNR) encodes a transcription factor able to bind and trans-activate its own promoter, Cell Growth Differ. 4, 1041-1050.
49. Poleev, A., Fickenscher, H., Mundlos, S., Winterpacht, A., Zabel, B., Fidler, A., Gruss, P. & Plachov, D. (1992) PAX8, a human paired box gene: isolation and expression in developing thyroid, kidney and Wilms' tumors, Development 116, 611-623.
50. Poleev, A., Wendler, F., Fickenscher, H., Zannini, M. S., Yaginuma, K., Abbot, K. & Plachov, D. (1995) Distinct functional properties of three human paired-box protein, PAX8, isoforms generated by alternative splicing in thyroid, kidney and Wilms' tumors, Eur. J. Biochem. 228, 899-911.
51. Rexach, M. & Blobel, G. (1995) Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins, Cell 83, 683-692.
52. Sadowski, I. & Ptashne, M. (1989) A vector for expressing GAL4 (1-147) fusions in mammalian cells, Nucleic Acids Res. 17, 7539.
53. Schafer, B. W., Czerny, T., Bernasconi, M., Genini, M. & Busslinger, M. (1994) Molecular cloning and characterization of a human PAX-7 cDNA expressed in normal and neoplastic myocytes, Nucleic Acids Res. 22, 4574-4582.
54. Segil, N., Bosema Roberts, S. & Heintz, N. (1991) Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity, Science 254, 1814-1816.
55. 2+-regulated transcription factor phosphorylated by calmodulin-dependent kinases, Science 252, 1427-1430.
56. Silver, P. A. (1991) How proteins enter the nucleus, Cell 64, 489-497.
57. Strachan, T. & Read, A. P. (1994) PAX genes, Curr. Opin. Genet. & Dev. 4, 427-438.
58. Stuart, E. T. & Gruss, P. (1995) PAX genes: what's new in developmental biology and cancer, Hum. Mol. Genet. 4, 1717-1720.
59. Stuart, E. T., Haffner, R., Oren, M. & Gruss, P. (1995) Loss of p53 function through PAX-mediated transcriptional repression, EMBO J. 14, 5638-5645.
60. Theill, L. E., Castrillo, J.-L, Wu, D. & Karin, M. (1989) Dissection of functional domains of the pituitary-specific transcription factor GHF-1, Nature 342, 945-948.
61. Tjian, R. & Maniatis, T. (1994) Transcriptional activation: a complex puzzle with few easy pieces, Cell 77, 5-8.
62. Towbin, H., Staehelin, T. & Gordon, J. (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications, Proc. Natl Acad. Sci. USA 76, 4350-4354.
63. Van Buskirk, R. V., Corcoran, T. & Wagner, J. A. (1985) Clonal variants of PC12 pheochromocytoma cells with defects in cAMP-dependent protein kinases induce ornithine decarboxylase in response to nerve growth factor but not to adenosine agonists, Mol. Cell. Biol. 5, 1984-1992.
64. Van Heuverswyn, B., Leriche, A., Van Sande, J., Dumont, J. E. & Vassart, G. (1985) Transcriptional control of thyroglobulin gene expression by cyclic AMP, FEBS Lett. 188, 192-196.
65. Van Renterghem, P., Vassart, G. & Chrisophe, D. (1996) Pax8 expression in primary cultured dog thyrocyte is increased by cyclic AMP, Biochim. Biophys. Acta 1307, 97-103.
66. Webster, N., Jin, J. R., Green, S., Hollis, M. & Chambon, P. (1988) The yeast UASG is a transcriptional enhancer in human HeLa cells in the presence of the GAL4 transactivator, Cell 52, 169-178.
67. Xu, W., Rould, M. A., Jun, S., Desplan, C. & Pabo, C. O. (1995) Crystal structure of a paired domain-DNA complex at 2.5 Å resolution reveals structural basis for Pax developmental mutations, Cell 80, 639-650.
68. Yamamoto, K. K., Gonzales, G. A., Biggs, W. H. & Montminy, M. R. (1988) Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB, Nature 334, 494-496.
69. Yankulov, K., Blau, J., Purton, T., Roberts, S. & Bentley, D. L. (1994) Transcriptional elongation by RNA polymerase II is stimulated by transactivators, Cell 77, 749-759.
70. Zannini, M. S., Francis-Lang, H., Plachov, D. & Di Lauro, R. (1992) Pax-8, a paired domain-containing protein, bind to a sequence overlapping the recognition site of a homeodomain and activates transcription from two thyroid-specific promoters, Mol. Cell. Biol. 12, 4230-4241.
71. Ziff, E. D. (1990) Transcription factors: a new family gathers at the cAMP resonse site, Trends Genet. 6, 69-72.