Penicillin-binding protein 2a of Streptococcus pneumoniae: Expression in Escherichia coli and purification and refolding of inclusion bodies into a soluble and enzymatically active enzyme

Protein Expression and Purification

Volumn 16, Issue 2, 1999, Pages 331-339

  Zhao, Genshi ^a   Meier, Timothy I ^a   Hoskins, Joann ^a   Jaskunas, S Richard ^a  

^a ELI LILLY AND COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO TERMINAL SEQUENCE; BACTERIAL CELL WALL; CENTRIFUGATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GAMMA GLUTAMYLTRANSFERASE; GLYCOSYLTRANSFERASE; PENICILLIN BINDING PROTEIN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; SOLUBILIZATION; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; STREPTOCOCCUS PNEUMONIAE;

EID: 0033166219     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1080     Document Type: Article

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