Differential expression of stathmin during neoplastic conversion of human prostate epithelial cells is reversed by hypomethylating agent, 5-azacytidine

International Journal of Oncology

Volumn 14, Issue 3, 1999, Pages 529-534

  Prasad, Sarada C ^a   Thraves, Peter J ^a   Soldatenkov, Viatcheslav A ^a   Varghese, Susan ^a   Dritschilo, Anatoly ^a  

^a GEORGETOWN UNIVERSITY   (United States)

Author keywords

2D PAGE; 5 azacytidine; CpG methylation; Neoplastic progression; Prostate carcinogenesis; Stathmin

Indexed keywords

ANTINEOPLASTIC ANTIMETABOLITE; AZACITIDINE; MICROTUBULE PROTEIN; PHOSPHOPROTEIN; STATHMIN; STMN1 PROTEIN, HUMAN;

ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL TRANSFORMATION; DNA METHYLATION; DRUG EFFECT; EPITHELIUM CELL; GENE EXPRESSION; HUMAN; MALE; METABOLISM; PATHOLOGY; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROSTATE; PROSTATE TUMOR;

ANTIMETABOLITES, ANTINEOPLASTIC; AZACITIDINE; CELL TRANSFORMATION, NEOPLASTIC; DNA METHYLATION; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITHELIAL CELLS; GENE EXPRESSION; HUMANS; MALE; MICROTUBULE PROTEINS; PHOSPHOPROTEINS; PHOSPHORYLATION; PROSTATE; PROSTATIC NEOPLASMS; STATHMIN; TUMOR CELLS, CULTURED;

EID: 0033090784     PISSN: 10196439     EISSN: None     Source Type: Journal    
DOI: 10.3892/ijo.14.3.529     Document Type: Article

References (32)

1. Kallioniemi OP and Visakorpi T: Genetic basis and clonal evolution of human prostate cancer. Adv Cancer Res 68: 225-255, 1996.
2. Isaacs WB: Molecular genetics of prostate cancer. Cancer Surv 25: 357-379, 1995.
3. Isaacs JT: Prostatic cancer: an overview. Cancer Metast Rev 12: 1-2, 1993.
4. Isaacs WB, Bova GS, Morton RA, Bussemakers MJ, Brooks JD and Ewing CM: Molecular biology of prostate cancer progression. Cancer Surv 23: 19-32, 1995.
5. Morton RJr, Watkins JJ, Bova GS, Wales MM, Baylin SB and Isaacs WB: Hypermethylation of chromosome 17p locus D17S5 in human prostate tissue. J Urol 156: 512-516, 1996.
6. Wang FL, Wang Y, Wong WK, Liu Y, Addivinola FJ, Liang P, Chen LB, Kantoff PW and Pardee AB: Two differentially expressed genes in normal human prostate tissue and in carcinoma. Cancer Res 56: 3634-3637, 1996.
7. Graff JR, Herman JG, Myohanen S, Baylin SB and Vertino PM: Mapping patterns of CpG island methylation in jormal and neoplastic cells implicates both upstream and downstream regions in de novo methylation. J Biol Chem 272: 22322-22329, 1997.
8. Baylin SB, Herman JG, Graff JR, Vertino PM and Lssa JP: Alterations in DNA methylation: a fundamental aspect of neoplasia. Adv Cancer Res 58: 141-196, 1998.
9. Schmutte C and Jones PA: Involvement of DNA methylation in human carcinogenesis. Biol Chem 379: 377-388, 1995.
10. Laird WP, JacksonGrusby L, Fazeli A, Dickinson LS, Jung EW, Li E, Weinberg AR and Jaenisch R: Suppression of intestinal neoplasia by DNA hypomethylation. Cell 81: 197-205, 1995.
11. Macleod R and Szyf M: Expression of antisense to DNA methyl transferase mRNA induces DNA demethyltion and inhibits tumorigenesis. J Biol Chem 270: 8037-8043, 1995.
12. Herman JG, Merlo A, Mao L, Lapidus RG, Issa JP, Davidson NE, Sidransky D and Baylin SB: Inactivation of the CDKN2/ p16/MESI gene is frequently associated with aberrant DNA methylation in all common human cancers. Cancer Res 55: 4525-4530, 1995.
13. Gonzalez-Zulueta M, Bender C, Yang AS, Nguyen T, Beart RW, Van Tornout JM and Jones PA: Methylation of the 5′ CpG island of the p16/CEKN2 tumor suppressor gene in normal and transformed human tissues correlates with gene silencing. Cancer Res 55: 4531-4535, 1995.
14. Akao T, Kakehi Y, Itoh N, Ozdemir E, Shimizu T, Tachibana A, Sasaki MS and Yoshida O: A high prevalence of functional inactivation by methylation modification of p16 INK4A/ CEKN2/MTS1 gene in primary urothelial cancers. Jpn J Cancer Res 88: 1078-1086, 1997.
15. Graff JR, Herman JG, Lapidus RG, Chopra H, Xu R, Jarrard DF, Isaacs WB, Pitha PM, Davidson NE and Baylin SB: E-cadherin expression is silenced by DNA hypermethylation in human breast and prostate carcinomas. Cancer Res 55: 5195-5199, 1995.
16. Yoshiura K, Kanai Y, Ochiai A, Shimoyama Y, Sugimura T and Hirohashi S: Silencing of the E-cadherin invasion-suppressor gene by CpG methylation in human carcinomas. Proc Natl Acad Sci USA 92: 7416-7419, 1995.
17. Nelson JB, Lee WH, Nguyen SH, Jarrad DF, Brooks JD, Magnuson SR, Opgenorth TJ, Nelson WG and Bova GS: Methylation of the 5′ CpG island of the endothelin B receptor gene is common in human prostate cancer. Cancer Res 57: 35-37,1997.
18. Muracami T, Li X, Gong J, Bhatia U, Traganos F and Darzynkiewicz Z: Inducton of apoptosis by 5-azacytidine: drug concentration-dependent differences in cell cycle specificity. Cancer Res 55: 3093-3098, 1995.
19. Caraglia M, Pinto A, Correale P, Zagonel V, Genua G, Leardi A, Pepe S, Bianco AR and Tagliaferri P: 5-aza-2′-deoxycytidine induces growth inhibition and upregulation of epidermal growth factor receptor on human epithelial cancer cells. Ann Oncol 5: 269-276, 1994.
20. Kuettel MR, Thraves P, Jung, Varghese S, Prasad S, Rhim J and Dritschilo A: Radiation-induced neoplastic transformation of human prostrate epithelial cells. Cancer Res 56: 5-10, 1996.
21. Prasad S, Thraves P, Dritschilo A and Kuettel M: Protein expression changes associated with radiation-induced neoplastic progression of human prostate epithelial cells. Electrophoresis 18: 629-637, 1997.
22. Prasad S, Thraves P, Dritschilo A and Kuettel M: Expression of cytokeratin 19 as a marker of neoplastic progression of human prostate epithelial cells. Prostate 35: 203-211, 1998.
23. Prasad S, Thraves P, Kuettel M and Dritschilo A: Cytoskeletal and adhesion protein changes during neoplastic progression of human prostate epithelial cells. Crit Rev Oncol Hematol 27: 69-80, 1998.
24. O'Farrel PH: High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250: 4007-4021, 1975.
25. Williams KR, Samandar SM, Stone KL, Saylor M and Rush J: Matrix assisted laser desorption ionization mass spectrometry as a complement to internal protein sequencing. In: The Protein Protocols Handbook. Walker JM (ed.) Humana Press, Totowa, pp541-555, 1996.
26. Celis JE, Rasmussen HH, Gromov P, Olsen E, Madsen P, Leffers H, Honore B, Dejgaard K, Vorum H, Kristensen DB, Ostergaard M, Haunso A, Jensen NA, Celis A, Basse B, Jauridsen JB, Ratz GP, Andersen AH, Walbum E, Kjaergaard I, Andersen I, Puype M, Damme J Ven and Vandekerckhove J: The human keratinocyte two-dimensional gel protein database (update 1995): mapping components of signal transduction pathways. Electrophoresis 16: 2177-2240, 1995.
27. Beretta L, Dubois MF, Sobel A and Bensaude O: Stathmin is a major substrate for mitogen-activated protein kinase during heat shock and chemical stress in HeLa cells. Eur J Biochem 227: 388-395, 1995.
28. Leighton IA, Curmi P, Campbell DG, Cohen P and Sobel A: The phosphorylation of stathmin by MAP kinase. Mol Cell Biochem 127: 151-156, 1993.
29. Marklund U, Larsson N, Gradin HM, Brattsand G and Gullberg M: Oncoprotein 18 is a phosphorylation-responsive regulator of microtubule dynamics. EMBO J 15: 5290-5298, 1996.
30. Guy GR, Philip R and Tan YH: Analysis of cellular phosphoproteins by two-dimensional gel electrophoresis: applications for cell signaling in normal and cancer cells. Electrophoresis 15: 417-440, 1994.
31. Nakamura K, Fujimoto M, Tanaka T and Fujikura Y: Differential expression of nucleophosmin and stathmin in human lymphoblastic cell lines, CCRF-CEM and JURKAT analyzed by two-dimensional gel electrophoresis. Electrophoresis 16: 1530-1535, 1995.
32. Wimmer K, Kuick R, Thorval D and Hanash SM: Two-dimensional separations of the genome and proteome of neuroblastoma cells. Electrophoresis 17: 1741-1751, 1996.