Purification and some chemical properties of 30 kDa ginkgo biloba glycoprotein, which reacts with antiserum against β1→2 xylose-containing N-Glycans

Bioscience, Biotechnology and Biochemistry

Volumn 63, Issue 3, 1999, Pages 463-467

  Kimura, Yoshinobu ^a   Harada, Takuo ^a   Matsuo, Sayuri ^a   Yonekura, Masami ^b  

^a OKAYAMA UNIVERSITY   (Japan)

^b IBARAKI UNIVERSITY   (Japan)

Author keywords

Antigenic N glycan; Antiserum against plant N glycan; Ginkgo biloba; Immunoblotting; Plant glycoprotein

Indexed keywords

ANTISERUM; GLYCOPROTEIN; POLYSACCHARIDE; PROTEIN HYDROLYSATE; XYLOSE;

AMINO ACID SEQUENCE; ARTICLE; CARBOHYDRATE ANALYSIS; CHEMISTRY; GINKGO BILOBA; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOBLOTTING; IMMUNOLOGY; ISOLATION AND PURIFICATION; MASS SPECTROMETRY; MEDICINAL PLANT; MOLECULAR GENETICS; MOLECULAR WEIGHT; PLANT SEED; POLYACRYLAMIDE GEL ELECTROPHORESIS;

AMINO ACID SEQUENCE; CARBOHYDRATE SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GINKGO BILOBA; GLYCOPROTEINS; IMMUNE SERA; IMMUNOBLOTTING; MOLECULAR SEQUENCE DATA; MOLECULAR WEIGHT; PLANTS, MEDICINAL; POLYSACCHARIDES; PROTEIN HYDROLYSATES; SEEDS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; XYLOSE;

EID: 0033089308     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.63.463     Document Type: Article

References (17)

1. Garda-Casado, G., Sanchez-Monge, R., Chrispeels, M. J., Armentia, A., Salcedo, G., and Gomez, L., Role of complex asparagine-linked glycans in the allergenicity of plant glycoproteins. Glycobiology, 6, 471-477 (1996).
2. Ogawa, H., Hijikata, A., Amano, M., Kojima, K., Fukushima, H., Ishizuka, I., Kurihara, Y., and Matsumoto, I., Structures and contribution to the antigenicity of oligosaccharides of Japanese cedar (Cryptomeria japónica) pollen allergen Cry j 1: relationship between the structures and antigenic epitopes of plant A-linked complex-type glycans. Glycoconjugate, J., 13, 555-566 (1996).
3. Ohsuga, H., Su, S-N., Takahashi, N., Yang, S-Y., Nakagawa, H., Shimada, I., Arata, Y., and Lee, Y. C., The carbohydrate moiety of the bermuda grass antigen BG60. J. Biol. Chem., 271, 26653-26658 (1996).
4. Armentia, A., Sanchez-Monge, R., Gomez, L., Barber, D., and Salcedo, G., In vivo allergenic activities of eleven purified members of a major allergen family from wheat and barley flour. Clin. Exp. Allergy, 23, 410-415 (1993).
5. Garda-Casado, G., Armentia, A., Sanchez-Monge, R., Sanchez, L. M., Lopez-Otin, C., and Salcedo, G., A major baker’s asthma allergen from rye flour is considerably more active than its barley counterpart. FEBS Lett., 364, 36-40 (1995).
6. Tsuji, H., Bando, N., Hiemori, M., Yamanishi, R., Kimoto, M., Nishikawa, K., and Ogawa, T., Purification and characterization of soybean allergen Gly m Bd 28 K. Biosci. Biotechnol. Biochem., 61, 942-947 (1997).
7. Kimura, Y., Matsuo, S., and Takagi, S., Enzymatic properties of a Ginkgo biloba endo-β-A-acetylglucosaminidase and A-gly-can structures of storage glycoproteins in the seeds. Biosci. Biotechnol. Biochem., 62, 253-261 (1998).
8. Kimura, Y., Iwata, K., Sumi, Y., and Takagi, S., Purification and substrate specificity of an endo-/LiV-acetylglucosaminidase from pea (Pisum sativum) seeds. Biosci. Biotechnol. Biochem., 60,228-232 (1996).
9. Kimura, Y., Tokuda, T., Ohno, A., Tanaka, H., and Ishiguro, Y., Enzymatic properties of endo- β-A-acetylglucosaminidase from developing tomato fruits and soybean seeds: substrate specificity of plant origin endoglycosidase. Biochim. Biophys. Acta., 1381, 27-36 (1998).
10. Lauriére, M., Lauriére, €., Chrispeels, M. J., Johnson, K. D., and Sturm, A., Characterization of a xylose-specific antiserum that reacts with the complex asparagine-linked glycans of extracellular and vacuolar glycoproteins. Plant. Physiol., 90, 1182-1188 (1989).
11. 4. J. Mol. Biol., 80, 575-599 (1973).
12. Zacharius, R. M., Zell, T. E., Morrison, J. H., and Woodlock, J. J., Glycoprotein staining following electrophoresis on acrylamide gels. Anal. Biochem., 30, 148-152 (1969).
13. Kondo, A., Suzuki, J., Kuraya, N., Hase, S., Kato, I., and Ikenaka, T., Improvement method for fluorescence labeling of sugar chains with sialic acid residues. Agrie. Biol. Chem., 54, 2169-2170 (1990).
14. Crestfield, A. M., Moore, S., Stein, W. H., The preparation and enzymatic hydrolysis of reduced and S-carboxymethylated proteins. J. Biol. Chem., 238, 622-627 (1963).
15. Kimura, Y., and Stadtman, T. C., Glycine reductase selenoprotein A is not a glycoprotein: The positive periodic-acid test is the result of peptide bond cleavage and carbonyl group generation. Proc. Natl. Acad. Sci. USA 92, 2189-2192 (1995).
16. Kimura, Y., Ohno, A., and Takagi, S., Structural analysis of N-glycans of storage glycoproteins in soybean (Glycine max) seed. Biosci. Biotechnol. Biochem., 61, 1866-1871 (1997).
17. 4-(acetyl-β-glucosaminyl)asparagine amidase from soybean (Glycine max) seeds: purification and substrate specificity. Biosci. Biotechnol. Biochem., 62, 412-418 (1998).