Direct determination of Helicobacter pylori vacA genotypes and cagA gene in gastric biopsies and relationship to gastrointestinal diseases

American Journal of Gastroenterology

Volumn 94, Issue 6, 1999, Pages 1525-1531

  Rudi, Jochen ^a   Rudy, Andreas ^a   Maiwald, Matthias ^b,c   Kuck, Dirk ^a   Sieg, Andreas ^a   Stremmel, Wolfgang ^a  

^a Stanford Medicine Children s Health   (United States)

^b UNIVERSITY OF HEIDELBERG   (Germany)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SIGNAL PEPTIDE;

ADULT; AGED; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DISEASE ASSOCIATION; DYSPEPSIA; FEMALE; GASTROINTESTINAL DISEASE; GENOTYPE; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; MUCOSA ASSOCIATED LYMPHOID TISSUE LYMPHOMA; NONHUMAN; PEPTIC ULCER; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STOMACH BIOPSY; STOMACH CANCER;

ADULT; AGED; AGED, 80 AND OVER; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BIOPSY; FEMALE; GASTROINTESTINAL DISEASES; GENOTYPE; HELICOBACTER PYLORI; HUMANS; MALE; MIDDLE AGED; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STOMACH;

EID: 0033050501     PISSN: 00029270     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1572-0241.1999.1138_a.x     Document Type: Article

References (32)

1. Warren J.R., Marshall B.J. Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet. i:1983;1273-1274.
2. Hentschel I., Brandstätter G., Dragosics B., et al. Effect of ranitidine and amoxicillin plus metronidazole on the eradication of Helicobacter pylori in the pathogenesis of duodenal and gastric ulcer. N Engl J Med. 328:1993;308-312.
3. Parsonnet J., Friedman G.D., Vandersteen D.P., et al. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med. 325:1991;1127-1131.
4. Wotherspoon A.C., Ortiz-Hidalgo C., Falzon M.R., et al. Helicobacter pylori-associated gastritis and primary B-cell gastric lymphoma. Lancet. 338:1991;1175-1176.
5. Leunk R.D., Johnson P.T., David B.C., et al. Cytotoxic activity in broth-culture filtrates of Campylobacter pylori. J Med Microbiol. 26:1988;93-99.
6. Figura N., Guglielmetti P., Rossolini A., et al. Cytotoxin production by Campylobacter pylori strains isolated from patients with peptic ulcers and from patients with chronic gastritis. J Clin Microbiol. 27:1984;225-226.
7. Cover T.L., Dooley C.P., Blaser M.J. Characterization of and human serologic response to proteins in Helicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect Immun. 58:1990;603-610.
8. Crabtree J.E., Taylor J.D., Wyatt J.I., et al. Mucosal IgA recognition of Helicobacter pylori 120 kDA protein, peptic ulceration and gastric pathology. Lancet. 59:1991;1264-1270.
9. Covacci A., Censini S., Bugnoli M., et al. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci USA. 90:1993;5791-5795.
10. Cover T.L., Cao P., Lind C.D., et al. Correlation between vacuolating cytotoxin production by Helicobacter pylori isolates in vitro and in vivo. Infect Immun. 61:1993;5008-5012.
11. Cover T.L., Tummuru M.K.R., Cao P., et al. Divergence of genetic sequences for the vacuolating cytotoxin among Helicobacter pylori strains. J Biol Chem. 269:1994;10566-10573.
12. Schmitt W., Haas R. Genetic analysis of the Helicobacter pylori vacuolating cytotoxin Structural similarities with the IgA protease type of exported protein . Mol Microbiol. 12:1994;307-319.
13. Harris R.P., Cover T.L., Crowe D.R., et al. Helicobacter pylori cytotoxin induces vacuolation of primary human mucosal epithelial cells. Infect Immun. 64:1996;4867-4871.
14. Goosens H., Glupczynski Y., Burette A., et al. Role of the vacuolating toxin from Helicobacter pylori in the pathogenesis of duodenal and gastric ulcer. Med Microbiol Lett. 1:1992;153-159.
15. Atherton J.C., Cao P., Peek R.M., et al. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. J Biol Chem. 270:1995;17771-17777.
16. Atherton J.C., Peek R.M., Tham K.T., et al. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology. 112:1997;92-99.
17. Rudi J., Kolb C., Maiwald M., et al. Diversity of Helicobacter pylori vacA and cagA genes and relationship to VacA and CagA protein expression, cytotoxin production, and associated diseases. J Clin Microbiol. 36:1998;944-948.
18. Censini S., Lange C., Xiang Z., et al. Cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc Natl Acad Sci USA. 93:1996;14648-14653.
19. Tummuru M.K.R., Sharma S.A., Blaser M.J. Helicobacter pylori picB, a homoloque of the Bordetella pertussis toxin secretion protein, is required for induction of IL-8 in gastric epithelial cells. Mol Microbiol. 18:1995;867-876.
20. Tummuru M.K.R., Cover T.L., Blaser M.J. Cloning and expression of a high-molecular-mass major antigen of Helicobacter pylori Evidence of linkage to cytotoxin production . Infect Immun. 61:1993;1799-1809.
21. Crabtree J.E., Figura N., Taylor J.D., et al. Expression of 120 kilodalton protein and cytotoxicity in Helicobacter pylori. J Clin Pathol. 45:1992;733-734.
22. Xiang Z., Censini S., Bayelli P.F., et al. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect Immun. 63:1995;94-98.
23. Rudi J., Kolb C., Maiwald M., et al. Serum antibodies against Helicobacter pylori proteins CagA and VacA are associated with increased risk for gastric adenocarcinoma. Dig Dis Sci. 42:1997;1652-1659.
24. Maiwald M., Kissel K., Srimuang S., et al. Comparison of polymerase chain reaction and conventional culture for the detection of legionellas in hospital water. J Appl Bacteriol. 76:1994;216-225.
25. Riley L.K., Franklin C.L., Hook R.R., et al. Identification of murine Helicobacters by PCR and restriction enzyme analyses. J Clin Microbiol. 34:1996;942-946.
26. Maiwald M., Ditton H.-J., Sonntag H.-G., et al. Characterization of contaminating DNA in Taq polymerase which occurs during amplification with a primer set for Legionella 5S ribosomal RNA. Mol Cell Prob. 8:1994;11-14.
27. Strobel S., Bereswill S., Balig P., et al. Identification and analysis of a new vacA genotype variant of Helicobacter pylori in different patient groups in Germany. J Clin Microbiol. 36:1998;1285-1289.
28. van Doorn L.J., Figueiredo C., Sanna R., et al. Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori. Gastroenterology. 115:1998;58-66.
29. van Doorn L.J., Figueiredo C., Rossau R., et al. Typing of Helicobacter pylori vacA gene and detection of cagA gene by PCR and reverse hybridization. J Clin Microbiol. 36:1998;1271-1275.
30. Atherton J.C., Cockayne A., Balsitis M., et al. Detection of intragastric sites at which Helicobacter pylori evades treatment with amoxycillin and cimetidine. Gut. 36:1995;670-674.
31. Weel J.F.L., van der Hulst R.W.M., Gerrits Y., et al. The interrelationship between cytotoxin-associated gene A, vacuolating cytotoxin, and Helicobacter pylori-related diseases. J Infect Dis. 173:1996;1171-1175.
32. Figura N., Vindigni C., Covacci A., et al. CagA positive and negative Helicobacter pylori strains are simultaneously present in the stomach of most patients with non-ulcer dyspepsia Relevance of histological damage . Gut. 42:1998;772-778.