cagA positive and negative Helicobacter pylori strains are simultaneously present in the stomach of most patients with non-ulcer dyspepsia: Relevance to histological damage

Gut

Volumn 42, Issue 6, 1998, Pages 772-778

  Figura, N ^a   Vindigni, C ^a   Covacci, A ^b   Presenti, L ^a   Burroni, D ^b   Vernillo, R ^a   Banducci, T ^a   Roviello, F ^a   Marrelli, D ^a   Biscontri, M ^a   Kristodhullu, S ^a   Gennari, C ^a   Vaira, D ^c  

^a UNIVERSITY OF SIENA   (Italy)

^b CHIRON VACCINES   (Italy)

^c UNIVERSITY OF BOLOGNA   (Italy)

Author keywords

CagA; Cell proliferation; Gastritis; Helicobacter pylori infection; Mucosal atrophy

Indexed keywords

ARTICLE; BACTERIUM IDENTIFICATION; CLINICAL ARTICLE; DISEASE ASSOCIATION; DYSPEPSIA; GRAM NEGATIVE INFECTION; HELICOBACTER PYLORI; HISTOPATHOLOGY; HUMAN; INTESTINE METAPLASIA; PRIORITY JOURNAL; STOMACH BIOPSY; STOMACH MUCOSA;

EID: 15644380740     PISSN: 00175749     EISSN: None     Source Type: Journal    
DOI: 10.1136/gut.42.6.772     Document Type: Article

References (35)

1. Blaser MJ. Gastric campylobacter-like organisms, gastritis and peptic ulcer disease. Gastroenterology 1987;93:371-83.
2. Parsonnet J, Friedman GD, Vandersteen DP, et al. Helicobacter pylori infection and the risk of gastric carcinoma. N Engl J Med 1991;325:1127-31.
3. Wotherspoon, AC, Ortiz Hidalgo C, Falzon MR, et al. Helicobacter pylori-associated gastritis and primary B-cell gastric carcinoma. Lancet 1991;338:1175-6.
4. Figura N, Guglielmetti P, Rossolini A, et al. Cytotoxin production by Campylobacter pylori strains isolated from patients with peptic ulcer and from patients with chronic gastritis only. J Clin Microbiol 1989;27:225-6.
5. Goossens H, Glupczynski Y, Burette A, et al. Role of the vacuolating toxin from Helicobacter pylori in the pathogenesis of duodenal and gastric ulcer. Medical Microbiology Letters 1992;1:153-9.
6. Crabtree JE, Taylor JD, Wyat JI, et al. Mucosal IgA recognition of Helicobacter pylori 120 kDa protein, peptic ulceration, and gastric pathology. Lancet 1991;338:332-5.
7. Phadnis SH, Ilver D, Janzon L, et at. Pathological significance and molecular characterization of the vacuolating toxin gene of Helicobacter pylori. Infect Immun 1994;62:1557-65.
8. Xiang Z, Censini S, Bayeli PF, et al. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that the clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating toxin. Infect Immun 1995;63:94-8.
9. Weel JFL, van der Hulst RWM, Gerrits Y, et al. The interrelationship between cytotoxin-associated gene A, vacuolating cytotoxin, and Helicobacter pylori-related diseases. J Infect Dis 1996;173:1171-5.
10. Oderda G, Figura N, Bayeli PF, et al. Serologic IgG recognition of Helicobacter pylon cytotoxin-associated protein, peptic ulcer and gastroduodenal pathology in childhood. Eur J Gastroenterol Hepatol 1993;5:695-9.
11. Figura N, Bugnoli M, Cusi MG, et al. Pathogenic mechanisms of Helicobacter pylori: production of cytotoxin. In: Malfertheiner P, Ditschuneit H, eds. Helicobacter pylori, gastritis and peptic ulcer. Berlin and Heidelberg: Springer-Verlag, 1990:86-95.
12. Kuipers FJ, Pérez-Pérez GI, Meuwissen SGM, et al. Helicobacter pylori and atrophic gastritis: importance of the cagA status. J Natl Cancer Inst 1995;87:1777-80.
13. Blaser MJ, Pérez-Pérez GI, Kleanthous H, et al. Infection with Helicobacter pylori strains possessing cagA is associated with an increased risk of developing adenocarcinoma of the stomach. Cancer Res 1995;55:2111-15.
14. Censini S, Lange C, Xiang Z, et al. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors. Proc Natl Acad Sci USA 1996;93:14648-53.
15. Fantry GT, Zheng QX, Darwin PE, et al. Mixed infection with cagA positive and cagA negative strains of Helicobacter pylon. Helicobacter 1996,1:98-106.
16. van der Ende A, Rauws EAJ, Feller M, et al. Heterogeneous Helicobacter pylori isolates from members of a family with a history of peptic ulcer disease. Gastroenterology 1996;111: 638-47.
17. Taylor NS, Fox JG, Akopyants NS, et al. Long-term colonization with single and multiple strains of Helicobacter pylori assessed by DNA fingerprinting. J Clin Microbiol 1995,33:918-23.
18. Owen RJ, Desai M, Figura N, et al. Comparison between degree of histological gastritis and DNA fingerprints, cytotoxicity and adhesivity of Helicobacter pylori from different gastric sites. Eur J Epidemiol 1993;9:315-21.
19. Glupczynski Y. Culture of Helicobacter pylori from gastric biopsies and antimicrobial susceptibility testing. In: Lee A, Mégraud F, eds. Helicobacter pylori: techniques for clinical diagnosis and basic research. London: Saunders, 1966:17-32.
20. Maniatis T, Fritsch EF, Sambrook J. Molecular cloning: a laboratory manual. 2nd ed. Plainview, NY: Cold Spring Harbor Laboratory Press, 1989.
21. Covacci A, Censini S, Bugnoli M, et al. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci USA 1993;90:5791-5.
22. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5.
23. Jorgensen M, Daskalopoulos G, Warburton V, et al. Multiple strain colonization and metronidazole resistance in Helicobacter pylori-infected patients: identification from sequential and multiple biopsy specimens. J Infect Dis 1996;174: 531-5.
24. Parsonnet J, Replogle M, Yang S, et al. Seroprevalence of CagA-positive strains among Helicobacter pylori-infected, healthy young adults. J Infect Dis 1997;175:1240-2.
25. Balzi D, Bidoli E, Franceschi S, et al. Estimates of cancer incidence and mortality in Italian Regions 1990. Associazi-one Italiana Registri Tumori A.I.R.T., 1997:36.
26. 2b Parsonnet J, Friedman GD, Orentreich N, et al. Risk for gastric cancer in people with CagA positive or CagA negative Helicobacter pylori infection. Gut 1997;40:297-301.
27. Muñoz N, Kato I, Peraza S, et al. Prevalence of precancerous lesions of the stomach in Venezuela. Cancer Epidemiol Biomarkers Prev 1996;5:41-6.
28. Correa P, Ruiz B, Shi T-Y, et al. Helicobacter pylori and nucleolar organizer regions in the gastric antral mucosa. Am J Clin Pathol 1994;101:656-60.
29. Bechi P, Balzi M, Becciolini A, et al. Helicobacter pylori and cell proliferation of the gastric mucosa: possible implication for gastric carcinogenesis. Am J Gastroenterol 1996;91:271-6.
30. De Koster E, Lage A, Buset M, et al. Influence of HP vacuolating toxin on gastric antrum and corpus mucosal cell proliferation status. Acta Gastroenterol Belg 1993;68 (suppl): 63.
31. Ricci V, Ciacci C, Zarrilli R, et al. Effect of Helicobacter pylori on gastric epithelial cell migration and proliferation in vitro: role of VacA and CagA. Infect Immun 1996;64: 2829-33.
32. Sandor Z, Vincze Á, Jadus M, et al. Effect of vac+ and vac-Helicobacter pylori (Hp) supernatants on the bioactivity of basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) in vitro. Gastroenterology 1996;110 (suppl):A249.
33. Brännström J, Zachrisson K, Engstrand L, et al. Helicobacter pylori stimulates DNA synthesis in a small intestinal cell line. Gastroenterology 1996;110(suppl):A871
34. Beales IL, Crabtree JE, Scunes D, et al. Antibodies to CagA are associated with gastric atrophy in Helicobacter pylori infection. Eur J Gastroenterol Hepatol 1996;8:645-9.
35. Ito S, Azuma T, Murakita H, et al. Profile of Helicobacter pylori cytotoxin derived from two areas of Japan with different prevalence of atrophic gastritis. Gut 1996;39:800-6.