Gene products required for surface expression of the capsular form of the group 1 K antigen in Escherichia coli (O9a:K30)

Molecular Microbiology

Volumn 31, Issue 5, 1999, Pages 1321-1332

  Drummelsmith, Jolyne ^a   Whitfield, Chris ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; GENE PRODUCT; GLYCOSYLTRANSFERASE; LIPOPOLYSACCHARIDE; MEMBRANE ANTIGEN; OLIGOSACCHARIDE; POLYSACCHARIDE;

ARTICLE; BACTERIAL MEMBRANE; BACTERIAL TRANSLOCATION; BIOSYNTHESIS; CYTOPLASM; ESCHERICHIA COLI; GENE CLUSTER; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0033017192     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1999.01277.x     Document Type: Article

References (68)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E., and Lipman, D.J. (1990) Basic local alignment search tool. J Mol Biol 215: 403-410.
2. Altschul, S.F., Madden, T.L., Schaffer, A.A., Zhang, J., Zhang, Z., Miller, W., and Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25: 3389-3402.
3. Arakawa, Y., Ohta, M., Wacharotayankun, R., Mori, M., Kido, N., Ito, H., et al. (1991) Biosynthesis of Klebsiella K2 capsular polysaccharide in Escherichia coli HB101 requires the function of rmpA and the chromosomal cps gene cluster of the virulent strain Klebsiella pneumoniae Chedid (O1:K2). Infect Immun 59: 2043-2050.
4. Arakawa, Y., Wacharotayankun, R., Nagatsuka, T., Ito, H., Kato, N., and Ohta, M. (1995) Genomic organization of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J Bacteriol 177: 1788-1796.
5. Bailey, M.J.A., Hughes, C., and Koronakis, V. (1997) RfaH and the cps element, components of a novel system controlling bacterial transcription elongation. Mol Microbiol 26: 845-851.
6. Becker, A., Niehaus, K., and Pühler, A. (1995) Low-molecular-weight succinoglycan is predominantly produced by Rhizobium meliloti strains carrying a mutated ExoP protein characterized by a periplasmic N-terminal domain and a missing C-terminal domain. Mol Microbiol 16: 191-203.
7. Becker, A., and Pühler, A. (1998) Specific amino acid substitutions in the proline-rich motif of the Rhizobium meliloti ExoP protein result in enhanced production of low-molecular-weight succinoglycan at the expense of high-molecular-weight succinoglycan. J Bacteriol 180: 395-399.
8. Beynon, L.M., Dumanski, A.J., McLean, R.J.C., MacLean, L.L., Richards, J.C., and Perry, M.B. (1992) Capsule structure in Proteus mirabilis (ATCC 49565). J Bacteriol 174: 2172-2177.
9. Binotto, J., MacLachlan, P.R., and Sanderson, P.R. (1991) Electrotransformation of Salmonella typhimurium LT2. Can J Microbiol 37: 474-477.
10. Chakraborty, A.K., Friebolin, H., and Stirm, S. (1980) Primary structure of the Escherichia coli serotype K30 capsular polysaccharide. J Bacteriol 141: 971-972.
11. Clarke, B.R., Bronner, D., Keenleyside, W.J., Severn, W.B., Richards, J.C., and Whitfield, C. (1995) Role of Rfe and RfbF in the initiation of biosynthesis of D-galactan I, the lipopolysaccharide O antigen from Klebsiella pneumoniae serotype O1. J Bacteriol 177: 5411-5418.
12. Claros, M.G., and von Hejne, G. (1994) Prediction of transmembrane segments in integral membrane proteins, and the putative topologies, using several algorithms. CABIOS 10: 685-686.
13. Cox, A., and Perry, M.B. (1996) Structural analysis of the O-antigen-core-region of the lipopolysaccharide from Vibrio cholerae O 139. Carbohydr Res 290: 59-65.
14. Dodgson, C., Amor, P., and Whitfield, C. (1996) Distribution of the rol gene encoding the regulator of lipopolysaccharide O-chain length in Escherichia coli and its influence on the expression of group I capsular K antigens. J Bacteriol 178: 1895-1902.
15. Drummelsmith, J., Amor, P.A., and Whitfield, C. (1997) Polymorphism, duplication, and IS 1-mediated rearrangement in the chromosomal his-rfb region of Escherichia coli strains with group IA capsular K antigens. J Bacteriol 179: 3232-3238.
16. Ebel, W., Vaughn, G.J., Peters, H.K. III, and Trempy, J.E. (1997) Inactivation of mdoH leads to increased expression of colanic acid capsular polysaccharide in Escherichia coli. J Bacteriol 179: 6858-6861.
17. Fält, I.C., Schweda, E.K.H., Weintraub, H., Sturm, S., Timmis, K.N., and Lindberg, A.A. (1993) Expression of the Shigella dysenteriae type-1 lipopolysaccharide repeating unit in Escherichia coli K-12/Shigella dysenteriae type-1 hybrids. Eur J Biochem 213: 573-581.
18. Frosch, M., Müller, D., Bousset, K., and Müller, A. (1992) Conserved outer membrane protein of Neisseria meningitidis involved in capsule expression. Infect Immun 60: 798-803.
19. Geremia, R.A., Petroni, E.A., Ielpi, L., and Henrissat, B. (1996) Towards a classification of glycosyltransferases based on amino acid sequence similarities: prokaryotic alpha-mannosyltransferases. Biochem J 318: 133-138.
20. Göhmann, S., Manning, P.A., Alpert, C.-A., Walker, M.J., and Timmis, K.N. (1994) Lipopolysaccharide O-antigen biosynthesis in Shigella dysenteriae serotype O1: analysis of the plasmid carried rfp determinant. Microb Pathog 16: 53-64.
21. Goldman, R.C., White, D., Ørskov, F., Ørskov, I., Rick, P.D., Lewis, M.S., et al. (1982) A surface polysaccharide of Escherichia coli O111 contains O-antigen and inhibits agglutination of cells by O-antiserum. J Bacteriol 151: 1210-1221.
22. Goldman, R.C., Joiner, K., and Leive, L. (1984) Serum-resistant mutants of Escherichia coli O111 contain increased lipopolysaccharide, lack an O antigen-containing capsule, and cover more of their lipid A-core with O antigen. J Bacteriol 159: 877-882.
23. González, J.E., York, G.M., and Walker, G.C. (1996) Rhizobium meliloti exopolysaccharides: synthesis and symbiotic function. Gene 179: 141-146.
24. Gottesman, S. (1995) Regulation of capsule synthesis modification of the two-component paradigm by an accessory unstable regulator. In: Two-Component Signal Transduction. Hoch, J.A., and Silhavy, T.J. (eds). Washington, DC: American Society for Microbiology Press, pp. 253-262.
25. Hamilton, C.A., Aldea, M., Washburn, B.K., Babtizke, P., and Kushner, S.R. (1989) New method of generating deletions and gene replacements is Escherichia coli. J Bacteriol 171: 4617-4622.
26. Hitchcock, P.J., and Brown, T.M. (1983) Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels. J Bacteriol 154: 269-277.
27. Hobbs, M., and Reeves, P.R. (1994) The JUMPstart sequence: a 39 bp element common to several polysaccharide gene clusters. Mol Microbiol 12: 855-856.
28. Huang, J., and Schell, M. (1995) Molecular characterization of the eps gene cluster of Pseudomonas solanacearum and its transcriptional regulation at a single promoter. Mol Microbiol 16: 977-989.
29. Jann, K., and Jann, B. (1997) Capsules of Escherichia coli. In: Escherichia Coli: Mechanisms of Virulence. Sussman, M. (ed.). Cambridge: Cambridge University Press, pp. 113-143.
30. Jayaratne, P., Bronner, D., MacLachlan, R., Dodgson, C., Kido, N., and Whitfield, C. (1994) Cloning and analysis of duplicated rfbK and rfbM genes involved in formation of GDP-mannose in Escherichia coli O9:K30 and participation of rfb genes in the synthesis of the group I K30 capsular polysaccharide. J Bacteriol 176: 3126-3139.
31. Johnson, J.G., and Wilson, D.B. (1977) Role of a sugar-lipid intermediate in colanic acid synthesis by Escherichia coli. J Bacteriol 129: 225-236.
32. Joiner, K.A. (1988) Complement evasion by bacteria and parasites. Annu Rev Microbiol 42: 201-230.
33. Keenleyside, W.J., Bronner, D., Jann, K., Jann, B., and Whitfield, C. (1993) Co-expression of colanic acid and serotype-specific capsular polysaccharides in Escherichia coli strains with group II K-antigens. J Bacteriol 175: 6725-6730.
34. Kido, N., Torgov, V.I., Sugiyama, T., Uchiya, K., Sugihara, H., Komatsu, T., et al. (1995) Expression of the O9 polysaccharide of Escherichia coli: sequencing of the E. coli O9 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport system. J Bacteriol 177: 2178-2187.
35. Kovach, M.E., Phillips, R.W., Elzer, P.H., Roop, R.M.I., and Peterson, K.M. (1994) pBBR1MCS: a broad-host-range cloning vector. Bio Techniques 16: 800-802.
36. Kuhn, H.-M., Meier-Dieter, U., and Mayer, H. (1988) ECA, the enterobacterial common antigen. FEMS Microbiol Rev 54: 195-222.
37. Liu, D., Haase, A.M., Lindqvist, L., Lindberg, A.A., and Reeves, P.R. (1993) Glycosyl transferases of O-antigen biosynthesis in Salmonella enterica: identification and characterization of transferase genes in groups B, C2, and E1. J Bacteriol 175: 3408-3413.
38. Liu, D., Cole, R.A., and Reeves, P.R. (1996) An O-antigen processing function for Wzx (RfbX): a promising candidate for O-unit flippase. J Bacteriol 178: 2102-2107.
39. MacLachlan, P.R., Keenleyside, W.J., Dodgson, C., and Whitfield, C. (1993) Formation of the K30 (group I) capsule in Escherichia coli O9:K30 does not require attachment to lipopolysaccharide lipid A-core. J Bacteriol 175: 7515-7522.
40. McCallum, K.L., Laakso, D.H., and Whitfield, C. (1989) Use of a bacteriophage-encoded glycanase enzyme in the generation of lipopolysaccharide O side chain deficient mutants of Escherichia coli O9: K30 and Klebsiella O1:K20: role of O and K antigens in resistance to complement-mediated serum killing. Can J Microbiol 35: 994-999.
41. Marolda, C.L., and Valvano, M.A. (1998) The promoter region of the Escherichia coli O7-specific lipopolysaccharide gene cluster: structural and functional characterization of an upstream untranslated mRNA sequence. J Bacteriol 180: 3070-3079.
42. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbour, New York: Cold Spring Harbour Laboratory Press.
43. Missiakas, D., Betton, J.-M., and Raina, S. (1996) New components of protein folding in extracytoplasmic compartments of Escherichia coli: SurA, FkpA and Skp/OmpH. Mol Microbiol 21: 871-884.
44. Mulford, C.A., and Osborn, M.J. (1983) An intermediate step in translocation of lipopolysaccharide to the outer membrane of Salmonella typhimurium. Proc Natl Acad Sci USA 80: 1159-1163.
45. Nieto, J.M., Bailey, M.J.A., Hughes, C., and Koronakis, V. (1996) Suppression of transcription polarity in the Escherichia coli haemolysin operon by a short upstream element shared by polysaccharide and DNA transfer determinants. Mol Microbiol 19: 705-713.
46. Osborn, M.J., Cynkin, M.A., Gilbert, J.M., Muller, L., and Singh, M. (1972) Synthesis of bacterial O-antigens. Methods Enzymol 28: 583-601.
47. Parker, C.T., Kloser, A.W., Schnaitman, C.A., Stein, M.A., Gottesman, S., and Gibson, B.W. (1992) Role of the rfaG and rfaP genes in determining the lipopolysaccharide core structure and cell surface properties of Escherichia coli K-12. J Bacteriol 174: 2525-2538.
48. Paulsen, I.T., Beness, A.M., and Saier, M.H. Jr. (1997) Computer-based analyses of the protein constituents of transport systems catalysing export of complex carbohydrates in bacteria. Microbiology 143: 2685-2699.
49. Peterson, A.A., and McGroarty, E.J. (1985) High-molecular-weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli. J Bacteriol 162: 738-745.
50. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
51. Saxena, I.M., Brown, R.M.J., Fevre, M., Geremia, R.A., and Henrissat, B. (1995) Multidomain architecture of betaglycosyltransferases: implications for mechanism of action. J Bacteriol 177: 1419-1424.
52. Schweizer, H.P. (1993) Small broad-host-range gentamycin resistance cessettes for site-specific insertion and deletion mutagenesis. Bio Techniques 15: 831-833.
53. Sledjeski, D.D., and Gottesman, S. (1996) Osmotic shock induction of capsule synthesis in Escherichia coli K-12. J Bacteriol 178: 1204-1206.
54. Stevens, M.P., Clarke, B.R., and Roberts, I.S. (1997) Regulation of the Escherichia coli K5 capsule gene cluster by transcription antitermination. Mol Microbiol 24: 1001-1012.
55. Stevenson, G., Neal, B., Liu, D., Hobbs, M., Packer, N.H., Batley, M., et al. (1994) Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb cluster. J Bacteriol 176: 4144-4156.
56. Stevenson, G., Andrianopoulos, K., Hobbs, M., and Reeves, P.R. (1996) Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid. J Bacteriol 178: 4885-4893.
57. Sutherland, I.W., and Norval, M. (1970) The synthesis of exopolysaccharide by Klebsiella aerogenes membrane preparations and the involvement of lipid intermediates. Biochem J 120: 567-576.
58. Towbin, M.T., Staehelin, T., and Gordon, G. (1979) Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350-4354.
59. Troy, F.A., Frerman, F.E., and Heath, E.C. (1971) The biosynthesis of capsular polysaccharide in Klebsiella aerogenes. J Biol Chem 246: 118-133.
60. Tsai, G.M., and Frasch, C.E. (1982) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal Biochem 119: 115-119.
61. Waldor, M.K., Colwell, R., and Mekalanos, J.J. (1994) The Vibrio cholerae O139 serogroup antigen includes an O-antigen capsule and lipopolysaccharide virulence determinants. Proc Natl Acad Sci USA 91: 11388-11392.
62. Wang, L., Curd, H., Qu, W., and Reeves, P.R. (1998) Sequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes. J Clin Microbiol 36: 3182-3187.
63. Whitfield, C. (1995) Biosynthesis of lipopolysaccharide O-antigens. Trends Microbiol 3: 178-185.
64. Whitfield, C., and Roberts, I.S. (1999) Structure, assembly and regulation of expression of capsules in Escherichia coli. Mol Microbiol 31: 1307-1319.
65. Whitfield, C., and Valvano, M.A. (1993) Biosynthesis and expression of cell surface polysaccharides in gram-negative bacteria. Adv Micro Physiol 35: 2589-2599.
66. Whitfield, C., Schoenhals, G., and Graham, L. (1989) Mutants of Escherichia coli O9: K30 with altered synthesis and expression of the capsular K antigen. J Gen Microbiol 135: 2589-2599.
67. Whitfield, C., Keenleyside, W.J., and Clarke, B.R. (1994). Structure, function and synthesis of cell surface polysaccharides in Escherichia coli. In: Escherichia Coli in Domestic Animals and Man. Gyles, C.L. (ed.). Wallingford: CAB International, pp. 437-494.
68. Whitfield, C., Amor, P.A., and Köplin, R. (1997) Modulation of surface architecture of Gram-negative bacteria by the action of surface polymer: lipidA-core ligase and by determinants of polymer chain length. Mol Microbiol 23: 629-638.