Regulation of the Escherichia coli K5 capsule gene cluster by transcription antitermination

Molecular Microbiology

Volumn 24, Issue 5, 1997, Pages 1001-1012

  Stevens, Mark P ^a,c   Clarke, Bradley R ^b   Roberts, Ian S ^b  

^a UNIVERSITY OF LEICESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

^c UNIVERSITY OF READING   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL POLYSACCHARIDE; RHO FACTOR;

ARTICLE; BACTERIAL MEMBRANE; ESCHERICHIA COLI; GENE CLUSTER; GENE EXPRESSION REGULATION; GENE MUTATION; NONHUMAN; OPERON; PRIORITY JOURNAL; PROMOTER REGION; REGULATOR GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION INITIATION; TRANSCRIPTION REGULATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030852077     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.4241780.x     Document Type: Article

References (67)

1. Arakawa, Y., Wacharotayankun, R., Nagatsuka, T., Ito, H., Kato, N., and Ohta, M. (1995) Genomic organisation of the Klebsiella pneumoniae cps region responsible for serotype K2 capsular polysaccharide synthesis in the virulent strain Chedid. J Bacteriol 177: 1788-1796.
2. Arba, H., Adhya, S., and De Crombruggle, B. (1981) Evidence for two gal promoters in intact Escherichia coli cells. J Biol Chem 256: 11905-11910.
3. Bailey, M.J.A., Koronakis, V., Schmoll, T., and Hughes, C. (1992) Escherichia coli HlyT protein, a transcriptional activator of haemolysin synthesis and secretion, is encoded by the rfaH (sfrB) locus required for expression of sex factor and lipopolysaccharide genes. Mol Microbiol 6: 1003-1012.
4. Beutin, L., and Achtman, M. (1979) Two Escherichia coli chromosomal cistrons, sfrA and sfrB, which are needed for expression of F-factor tra functions. J Bacteriol 139: 730-737.
5. Beutin, L., Manning, P.A., Achtman, M., and Willets, N. (1981) sfrA and sfrB products of Escherichia coli K-12 are transcriptional control factors. J Bacteriol 145: 840-844.
6. 35S label as an aid to rapid DNA sequence determination. Proc Natl Acad Sci USA 80: 3963-3965.
7. Bliss, J.M., and Silver, R.P. (1996) Coating the surface: a model for expression of capsular polysialic acid in Escherichia coli K1. Mol Microbiol 21: 221-231.
8. Brazas, R., Davie, E., Farewell, A., and Rothfield, L.I. (1991) Transcriptional organisation of the rfaGBIJ locus of Salmonella typhimurium. J Bacteriol 173: 6168-6173.
9. Bronner, D., Sieberth, V., Pazzani, C., Roberts, I.S., Boulnois, G.J, Jann, B., and Jann, K. (1993) Expression of the capsular K5 polysaccharide of Escherichia coli: biochemical and electron microscopic analyses of mutants with defects in region 1 of the K5 gene cluster. J Bacteriol 175: 5984-5992.
10. Brosius, J. (1984) Plasmid vectors for the selection of promoters. Gene 27: 151-160.
11. Burns, C.M., and Richardson, J.P. (1995) NusG is required to overcome a kinetic limitation to Rho function at an intragenic terminator. Proc Natl Acad Sci USA 92: 4738-4742.
12. Burova, E., and Gottesman, M.E. (1995) NusG overexpression inhibits Rho-dependent termination in Escherichia coli. Mol Microbiol 17: 633-641.
13. Burova, E., Hung, S.C., Sagitov, V., Stitt, B.L., and Gottesman, M.E. (1995) Escherichia coli NusG protein stimulates transcription elongation rates in vivo and in vitro. J Bacteriol 177: 1388-1392.
14. Chang, A.Y.C., and Cohen, S.N. (1978) Construction and characterisation of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134: 1141-1156.
15. Cieslewicz, M., and Vimr, E. (1996) Thermoregulation of kpsF, the first region 1 gene in the kps locus for polysialic acid biosynthesis in Escherichia coli K1. J Bacteriol 178: 3212-3220.
16. Cieslewicz, M., Steenbergen, S., and Vimr, E. (1993) Cloning, sequencing, expression and complementation analysis of the Escherichia coli K1 kps region 1 gene, kpsE, and identification of an upstream open reading frame with homology to GutQ. J Bacteriol 175: 8018-8023.
17. Clackson, T., Güssow, D., and Jones, P.T. (1991) General application of PCR to gene cloning and manipulation. In PCR: A Practical Approach. McPherson, M., Quirke, P., and Taylor, G. (eds). Oxford: IRL Press, pp. 187-214.
18. Das, A. (1992) How the phage lambda N gene product suppresses transcription termination: communication of RNA polymerase with regulatory proteins mediated by signals in the nascent RNA. J Bacteriol 174: 6711-6716.
19. Devereux, J., Haeberli, P., and Smithies, O. (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387-395.
20. Donnenberg, M.S., and Kaper, J.B. (1991) Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect Immun 59: 4310-4317.
21. Farewell, A., Brazas, R., Davie, E., Mason, J., and Rothfield, L.I. (1991) Suppression of the abnormal phenotype of Salmonella typhimurium rfaH mutants by mutations in the gene for transcription termination factor Rho. J Bacteriol 173: 5188-5193.
22. Friedman, D.I., and Court, D.L. (1995) Transcription antitermination: the λ paradigm updated. Mol Microbiol 18: 191-200.
23. Gaffney, D., Skurray, R., and Willets, N. (1983) Regulation of the F conjugation genes studied by hybridisation and tralacZ fusion. J Mol Biol 168: 103-122.
24. Grayhack, E.J., Yang, X., Lau, L.F., and Roberts, J.W. (1985) Phage lambda gene Q antiterminator recognises RNA polymerase near the promoter and accelerates it through a pause site. Cell 42: 259-269.
25. Gilliland, G., Perron, S., and Bunn, F. (1990) Competitive PCR for quantitation of mRNA. In PCR Protocols. Innes, M., Gelford, D., Smirsky, J., and White, T. (eds). San Diego: Academic Press Inc., pp. 60-69.
26. Higuchi, R. (1990) Recombinant PCR. In PCR Protocols. Innes, M., Gelford, D., Smirsky, J., and White, T. (eds). San Diego: Academic Press Inc., pp. 177-183.
27. Hobbs, M., and Reeves, P.R. (1994) The JUMPstart sequence: a 39 bp element common to several polysaccharide gene clusters. Mol Microbiol 12: 855-856.
28. Jann, B., and Jann, K. (1990) Structure and biosynthesis of the capsular antigens of Escherichia coli. Curr Top Microbiol Immunol 150: 19-42.
29. Jin, D.J., Burgess, R.R., Richardson, J.P., and Gross, C.A. (1992) Termination efficiency at Rho-dependent terminators depends on kinetic coupling between RNA polymerase and Rho. Proc Natl Acad Sci USA 89: 1453-1457.
30. Kawasaki, E.S., Clark, S.S., Coyne, M.Y., Smith, S.D., Champlin, R., Witte, O.N., and McCormick, F.P. (1988) Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Proc Natl Acad Sci USA 85: 5698-5702.
31. Koronakis, V., Cross, M., and Hughes, C. (1988) Expression of the E. coli hemolysin secretion gene hlyB involves transcript anti-termination within the hly operon. Nucleic Acids Res 16: 4789-4800.
32. Koronakis, V., Cross, M., and Hughes, C. (1989) Transcription antitermination in an Escherichia coli haemolysin operon is directed progressively by cis-acting DNA sequences upstream of the promoter region. Mol Microbiol 3: 1397-1404.
33. Kröncke, K., Golecki, J., and Jann, K. (1990) Further electron microscopic studies on the expression of Escherichia coli group Il capsules. J Bacteriol 172: 3469-3472.
34. Leeds, J.A., and Welch, R.A. (1996) RfaH enhances elongation of Escherichia coli hlyCABD mRNA. J Bacteriol 178: 1850-1857.
35. Li, J., Horwitz, R.J., McCracken, S., and Greenblatt, J. (1992) NusG, a new Escherichia coli elongation factor involved in transciption antitermination by the N protein of phage λ. J Biol Chem 267: 6012-6019.
36. Li, J., Mason, S.W., and Greenblatt, J. (1993) Elongation factor NusG interacts with termination factor ρ to regulate termination and antitermination of transcription. Genes Dev 7: 161-172.
37. Mason, S.W., Li, J., and Greenblatt, J. (1992) Host factor requirements for processive antitermination and suppression of pausing by the N protein of bacteriophage λ. J Biol Chem 267: 19418-19426.
38. Mead, D., Szezesna-Skorupa, E., and Kemper, B. (1986) Single-stranded DNA 'blue' T7 promoter plasmids: a versatile promoter system for cloning and protein engineering. Protein Eng 1: 67-74.
39. Nataro, J.P., and Levine, M.M. (1994) Escherichia coli diseases in humans. In Escherichia coli In Domestic Animals and Humans. Gyles, C. (ed.). Oxford: CAB International Press, pp. 285-333.
40. Nieto, J.M., Bailey, M.J.A., Hughes, C., and Koronakis, V. (1996) Suppression of transcription polarity in the Escherichia coli haemolysin operon by a short upstream element shared by polysaccharide and DNA transfer determinants. Mol Microbiol 19: 705-713.
41. Ørskov, F., and Ørskov, I. (1992) Escherichia coli serotyping and disease in man and animals. Can J Microbiol 38: 699-704.
42. Pavelka, M.S., Wright, L.F., and Silver, R.P. (1991) Identification of two genes, kpsM and kpsT, in region 3 of the polysialic acid gene cluster of Escherichia coli K1. J Bacteriol 173: 4603-4610.
43. Pazzani, C., Rosenow, C., Boulnois, G.J., Bronner, D., Jann, K., and Roberts, I.S. (1993) Molecular analysis of region 1 of the Escherichia coli K5 capsule gene cluster: a region involved in cell surface expression of capsular polysaccharide. J Bacteriol 175: 5978-5983.
44. Peabody, D.S., Andrews, C.L., Escudero, K.W., Devine, J.H., Baldwin, T.O., and Bear, D.G. (1989) A plasmid vector and quantitative techniques for the study of transcription termination in Escherichia coli using bacterial luciferase. Gene 75: 289-296.
45. Petit, C., Rigg, G.P., Pazzani, C., Smith, A., Sieberth, V., Stevens, M., Boulnois, G., Jann, K., and Roberts, I.S. (1995) Region 2 of the Escherichia coli K5 capsule gene cluster encoding proteins for the biosynthesis of the K5 polysaccharide. Mol Microbiol 17: 611-620.
46. Pradel, E., and Schnaitman, C.A. (1991) Effect of rfaH(sfrB) and temperature on expression of rfa genes of Escherichia coli K-12. J Bacteriol 173: 6428-6431.
47. Railing, G., and Linn, T. (1987) Evidence that Rho and NusA are involved in termination in the rplL-rpoB intercistronic region. J Bacteriol 169: 2277-2280.
48. Rehemtulla, A., Kadam, S., and Sanderson, K. (1986) Cloning and analysis of the sfrB (sex factor repression) gene of Escherichia coli K-12. J Bacteriol 166: 651-657.
49. Roberts, I.S. (1995) Bacterial polysaccharides in sickness and in health. J Gen Microbiol 141: 2023-2031.
50. Roberts, I., Mountford, R., High, N., Bitter-Suermann, D., Jann, K., Timmis, K., and Boulnois, G. (1986) Molecular cloning and analysis of genes for the production of the K5, K7, K-12 and K92 capsular polysaccharides in Escherichia coli. J Bacteriol 170: 1305-1310.
51. Roberts, I.S, Mountford, R., Hodge, R., Jann, K., and Boulnois, G.J. (1988) Common organisation of gene clusters for production of different capsular polysaccharides (K antigens) in Escherichia coli. J Bacteriol 170: 1305-1310.
52. Rosenow, C., Esumeh, F., Roberts, I.S., and Jann, K. (1995a) Characterisation and localisation of the KpsE protein of Escherichia coli K5, which is involved in polysaccharide export. J Bacteriol 177: 1137-1143.
53. Rosenow, C., Roberts, I.S., and Jann, K. (1995b) Isolation from recombinant Escherichia coli and characterisation of CMP-Kdo synthetase, involved in the expression of the capsular K5 polysaccharide (K-CKS). FEMS Microbiol Lett 125: 159-164.
54. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual. 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
55. Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467.
56. Shaw, W.V. (1975) Chloramphenicol acetyl transferase from chloramphenicol resistant bacteria. Meth Enzymol 43: 737-755.
57. Sieberth, V., Rigg, G.P., Roberts, I.S., and Jann, K. (1995) Expression and characterisation of UDPGIc dehydrogenase (KfiD) which is encoded in the type-specific region 2 of the Escherichia coli K5 capsule genes. J Bacteriol 177: 4562-4565.
58. Silver, R.P. (1994) Genetic analysis of the Escherichia coli K1 capsule gene cluster. In Molecular Genetics of Bacterial Pathogenesis. Miller, V., Kaper, J., Portnoy, D., and Isberg, R. (eds). Washington, USA: American Society for Microbiology Press, pp. 313-326.
59. Simpson, D.A., Hammarton, T.C., and Roberts, I.S. (1996) Transcriptional organisation and regulation of expression of region 1 of the Escherichia coli K5 capsule gene cluster. J Bacteriol 178: 6466-6474.
60. Smith, A.N., Boulnois, G.J., and Roberts, I.S. (1990) Molecular analysis of the Escherichia coli K5 kps locus: identification and characterisation of an inner-membrane capsular polysaccharide transport system. Mol Microbiol 4: 1863-1869.
61. Stevens, M.P. (1995) Regulation of Escherichia coli K5 capsular polysaccharide expression. PhD Thesis. (JR) University of Leicester, Leicester, UK.
62. Stevens, M.P., Hänfling, P., Jann, B., Jann, K., and Roberts, I.S. (1994) Regulation of Escherichia coli K5 capsular polysaccharide expression: evidence for involvement of RfaH in the expression of group II capsules. FEMS Microbiol Lett 124: 93-93.
63. Sullivan, S.L., and Gottesman, M.E. (1992) Requirement for E. coli NusG protein in factor-dependent transcription termination. Cell 68: 989-994.
64. Vogel, M., Hess, J., Then, I., Juárez, A., and Goebel, W. (1988) Characterisation of a sequence (hlyR) which enhances synthesis and secretion of hemolysin in Escherichia coli. Mol Gen Genet 212: 76-84.
65. Wunder, D.E., Aaronson, W., Hayes, S.F., Bliss, J.M., and Silver, R.P. (1994) Nucleotide sequence and mutational analysis of the gene encoding KpsD, a periplasmic protein involved in the transport of polysialic acid in Escherichia coli K1. J Bacteriol 176: 4025-4033.
66. Yanisch-Perron, C., Vieira, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of M13mp18 and pUC19 vectors. Gene 33: 103-119.
67. Yarnell, W.S., and Roberts, J.W. (1992) The phage λ gene Q transcription antiterminator binds DNA in the late gene promoter as it modifies RNA polymerase. Cell 69: 1181-1189.