Distribution of the rol gene encoding the regulator of lipopolysaccharide O-chain length in Escherichia coli and its influence on the expression of group I capsular K antigens

Journal of Bacteriology

Volumn 178, Issue 7, 1996, Pages 1895-1902

  Dodgson, Christine ^a   Amor, Paul ^a   Whitfield, Chris ^a  

^a UNIVERSITY OF GUELPH   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BACTERIUM LIPOPOLYSACCHARIDE;

ANTIGEN EXPRESSION; ARTICLE; BACTERIAL GENE; ESCHERICHIA COLI; GENE CONTROL; GENE EXPRESSION REGULATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; REGULATORY MECHANISM; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); ENTEROBACTERIACEAE; ESCHERICHIA COLI;

EID: 0029996473     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.7.1895-1902.1996     Document Type: Article

References (56)

1. Bastin, D. A., G. Stevenson, P. K. Brown, A. Haase, and P. R. Reeves. 1993. Repeat unit polysaccharides of bacteria, a model for polymerization resembling that of ribosomes and fatty acid synthetase, with a novel mechanism for determining chain length. Mol Microbiol. 7:725-734.
2. Batchelor, R. A., P. Alifano, E. Biffali, S. I. Hull, and R. A. Hull. 1992. Nucleotide sequences of the genes regulating O-polysaccharide chain length (rol) from Escherichia coli and Salmonella typhimurium: protein homology and functional complementation J. Bacteriol 174:5228-5236
3. Batchelor, R. A., G. E. Haraguchi, R. A. Hull, and S. I. Hull. 1991. Regulation by a novel protein of the bimodal distribution of lipopolysaccharide in the outer membrane of Escherichia coli. J. Bacteriol. 173:5699-5704.
4. Beynon, L. M., A. J. Dumanski, R. J. C. McLean, L. L. MacLean, J. C. Richards, and M. B. Perry. 1992. Capsule structure in Proteus mirabilis (ATCC 49565). J. Bacteriol. 174:2172-2177
5. Binotto, J., P. R. MacLachlan, and P. R. Sanderson. 1991. Electrotransformation of Salmonella typhimurium LT2. Can J. Microbiol. 37:474-477.
6. Boulnois, G. J., and I. S. Roberts. 1990 Genetics of capsular polysaccharide production in bacteria. Curr Top. Microbiol. Immunol. 150:1-18.
7. Bronner, D., B. R. Clarke, and C. Whitfield. 1994. Identification of an ATP-binding cassette transport system required for translocation of lipopolysaccharide O-antigen side chains across the cytoplasmic membrane of Klebsiella pneumoniae serotype O1 Mol. Microbiol 14:505-519.
8. Clarke, A. J., V. Sarabia, W. Keenleyside, P. R. MacLachlan, and C. Whitfield. 1991 The compositional analysis of bacterial extracellular polysaccharides by high-performance anion-exchange chromatography. Anal. Biochem. 199:68-74.
9. Clarke, B. R., and C. Whitfield. 1992. Molecular cloning of the rfb region of Klebsiella pneumoniae serotype O1:K20: the rfb gene cluster is responsible for synthesis of the D-galactan I O polysaccharide. J. Bacteriol. 174:4614-4621
10. Dodgson, C., and C. Whitfield. Unpublished data
11. Dunn, S. D. 1986. Effects of the modification of transfer buffer composition and the renaturation of proteins in gels on recognition of proteins on Western blots by monoclonal antibodies. Anal. Biochem. 157:144-153
12. Dutton, G. G. S., and L. A. S. Parolis. 1989 Polysaccharide antigens of Escherichia coli, p 223-240. In I. C M. Dea and S. S. Stivola (ed.), Recent developments in industrial polysaccharides: biological and biotechnological advances Gordon and Breach Science Publishers, New York.
13. Franco, A. V., D. Liu, and P. R. Reeves. 1996. A Wzz (Cld) protein determines the chain length of K lipopolysaccharide in Escherichia coli O8 and O9 strains. J. Bacteriol 178:1903-1907
14. Frosch, M., U. Edwards, K. Bousset, B. Krausse, and C. Weisgerber. 1991 Evidence of a common molecular origin of the capsule gene loci in gram-negative bacteria expressing group II capsular polysaccharides Mol. Microbiol. 5:1251-1263.
15. Goldman, R. C., K. Joiner, and L. Leive. 1984. Serum-resistant mutants of Escherichia coli O111 contain increased lipopolysaccharide, lack an O antigen-containing capsule, and cover more of their lipid A core with O antigen. J. Bacteriol. 159:877-882.
16. Goldman, R. C., D. White, F. Ørskov, I. Ørskov, P. D. Rick, M. S. Lewis, A. K. Bhattacharjee, and L. Leive. 1982 A surface polysaccharide of Escherichia coli O111 contains O-antigen and inhibits agglutination of cells by O-antiserum J. Bacteriol. 151:1210-1221
17. Hitchcock, P. J., and T. M. Brown. 1983 Morphological heterogeneity among Salmonella lipopolysaccharide chemotypes in silver-stained polyacrylamide gels J. Bacteriol 154:269-277.
18. Jann, B., and K. Jann. 1990. Structure and biosynthesis of the capsular antigens of Escherichia coli. Curr Top. Microbiol. Immunol. 150:19-42.
19. Jann, K., T. Dengler, and B. Jann. 1992 Core-lipid A on the K40 polysaccharide of Escherichia coli O8:K40:H9, a representative of group I capsular polysaccharides. Zentrabl Bakteriol 276:196-204
20. Jayaratne, P., D. Bronner, R. MacLachlan, C. Dodgson, N. Kido, and C. Whitfield. 1994 Cloning and analysis of duplicated rfbK and rfbM genes involved in formation of GDP-mannose in Escherichia coli O9:K30 and participation of rfb genes in the synthesis of the group I K30 capsular polysaccharide. J. Bacteriol. 176:3126-3139
21. Jayaratne, P., W. J. Keenleyside, P. R. MacLachlan, C. Dodgson, and C. Whitfield. 1993. Characterization of rcsB and rcsC from Escherichia coli O9:K30:H12 and examination of the role of the rcs regulatory system in expression of group I capsular polysaccharides. J. Bacteriol 175:5384-5394.
22. Joiner, K. A. 1985. Studies on the mechanism of bacterial resistance to complement-mediated killing and the mechanism of action of bactericidal antibody. Curr. Top. Microbiol. Immunol. 121:99-133.
23. Joiner, K. A. 1988. Complement evasion by bacteria and parasites. Annu. Rev. Microbiol. 42:201-230.
24. Kido, N., V. I. Torgov, T. Sugiyama, K. Uchiya, H. Sugihara, T. Komatsu, N. Kato, and K. Jann. 1995. Expression of the O9 polysaccharide of Escherichia colt: sequencing of the E. coli O9 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport system. J Bacteriol. 177:2178-2187.
25. Klena, J. D., and C. A. Schnaitman. 1993. Function of the rfb gene cluster and the rfe gene in the synthesis of O antigen by Shigella dysenteriae 1. Mol Microbiol. 9:393-402.
26. Krallmann-Wenzel, U., and G. Schmidt. 1994. A simple procedure to demonstrate the presence of the O-antigen capsule in enteropathogenic Escherichia coli. Zentralbl. Bakteriol. 281:140-145.
27. Kroll, J. S., B. Loynds, L. N. Brophy, and E. R. Moxon. 1990. The bex locus in encapsulated Haemophilus influenzae: a chromosomal region involved in capsule polysaccharide export. Mol. Microbiol. 4:1853-1862
28. Kuhn, H.-M., U. Meier-Dieter, and H. Mayer. 1988. ECA, the enterobacterial common antigen. FEMS Microbiol. Rev. 54:195-222.
29. Laakso, D. H., M. K. Homonylo, S. J. Wilmot, and C. Whitfield. 1988. Transfer and expression of the genetic determinants for O and K antigen synthesis in Escherichia coli O9.K(A)30:H12 and Klebsiella sp. O1.K20, in Escherichia coli K-12. Can. J. Microbiol. 34:987-992.
30. MacLachlan, P. R., W. J. Keenleyside, C. Dodgson, and C. Whitfield. 1993 Formation of the K30 (group I) capsule in Escherichia coli O9:K30 does not require attachment to lipopolysaccharide lipid A-core. J Bacteriol. 175: 7515-7522.
31. Marolda, C. L., and M. A. Valvano. 1993 Identification, expression, and DNA sequence of the GDP-mannose biosynthesis genes encoded by the O7 rfb gene cluster of strain VW187 (Escherichia coli O7 K1). J. Bacteriol. 175: 148-158
32. McCallum, K. L., D. H. Laakso, and C. Whitfield. 1989 Use of a bacteriophage-encoded glycanase enzyme in the generation of lipopolysaccharide O side chain deficient mutants of Escherichia coli O9:K30 and Klebsiella O1: K20. role of O and K antigens in resistance to complement-mediated serum killing. Can. J. Microbiol. 35:994-999.
33. McGrath, B. C., and M. J. Osburn. 1991. Localization of terminal steps of O-antigen synthesis in Salmonella typhimurium. J. Bacteriol. 173:649-654.
34. Meier, U., and H. Mayer. 1985. Genetic location of genes encoding enterobacterial common antigen. J. Bacteriol. 163:756-762.
35. Meler-Dieter, U., R. Starman, K. Barr, H. Mayer, and P. D. Rick. 1990. Biosynthesis of enterobacterial common antigen in Escherichia coli. Biochemical characterization of Tn10 insertion mutants defective in enterobacterial common antigen synthesis. J. Biol. Chem. 265:13490-13497.
36. Miller, J. H. 1992. A short course in bacteria genetics A laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, Plainview, N.Y.
37. Morona, R., L. Van Den Bosch, and P. A. Manning. 1995. Molecular, genetic, and topological characterization of O-antigen chain length regulation in Shingella flexneri. J. Bacteriol. 177:1059-1068.
38. Neuhard, J., and E. Thomassen. 1976. Altered deoxynucleotide pools in P2 eductants of Escherichia coli K-12 due to the deletion of the dcd gene. J. Bacteriol 126:999-1001.
39. Parker, C. T., A. W. Kloser, C. A. Schnaitman, M. A. Stein, S. Gottesman, and B. W. Gibson. 1992 Role of the rfaG and rfaP genes in determining the lipopolysaccharide core structure and cell surface properties of Escherichia coli K-12. J. Bacteriol. 174:2525-2538.
40. Pavelka, M. J., L. F. Wright, and R. P. Silver. 1991. Identification of two genes, kpsM and kpsT, in region 3 of the polysialic acid gene cluster of Escherichia coli K1. J. Bacteriol. 173:4603-4610.
41. Pavelka, M. S. J., S. F. Hayes, and R. P. Silver. 1994. Characterization of KpsT, the ATP-binding component of the ABC-transporter invoked with the export of capsular polysialic acid in Escherichia coli K1. J. Biol. Chem 269:20149-20158.
42. Peterson, A. A., and E. J. McGroarty. 1985. High-molecular-weight components in lipopolysaccharides of Salmonella typhimurium, Salmonella minnesota, and Escherichia coli. J. Bacteriol. 162:738-745.
43. Reeves, P. R. 1993. Evolution of Salmonella O antigen variation by interspecific gene transfer on a large scale. Trends Genet. 9:17-22
44. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989 Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
45. Schmidt, G., B. Jann, K. Jann, I. Ørskov, and F. Ørskov. 1977 Genetic determinants of the synthesis of the polysaccharide capsular antigen K27(A) of Escherichia coli. J. Gen. Microbiol. 100:355-361.
46. Silver, R. P., P. Annunziato, M. S. Pavelka, R. P. Pidgeon, L. F. Wright, and D. E. Wunder. 1992. Genetic and molecular analyses of the polysialic acid gene cluster of Escherichia coli K1, p. 59-71. In J. Roth, U. Rutishauser, and F. A. I. Troy (ed.), Polysialic acid from microbes to man. Advances in Life Sciences series. Birkhäuser Verlag, Basel
47. Smith, A. N., G. J. Boulnois, and I. S. Roberts. 1990. Molecular analysis of the Escherichia coli K5 kps locus: identification and characterization of an inner-membrane capsular polysaccharide transport system. Mol. Microbiol. 4:1863-1869.
48. Sturm, S., and K. N. Timmis. 1986. Cloning of the rfb region of Slugella dysentertae 1 and construction of an rfb-rfp gene cassette for the development of lipopolysaccharide-based live anti-dysentery vaccines. Microb Pathog. 1: 289-297.
49. Szabo, M., D. Bronner, and C. Whitfield. 1995. Relationships between the rfb gene clusters required for biosynthesis of identical D-galactose-containing O antigens in Klebsiella pneumoniae serotype O1 and Serratta marcescens serotype O20. J. Bacteriol. 177:1544-1553.
50. Towbin, M. T., T. Staehelin, and G. Gordon. 1979. Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets, procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354
51. Tsai, G. M., and C. E. Frasch. 1982. A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels. Anal. Biochem. 119:115-119.
52. Whitfield, C. 1995. Biosynthesis of lipopolysaccharide O-antigens. Trends Microbiol 3:178-185.
53. Whitfield, C., W. J. Keenleyside, and B. R. Clarke. 1994. Structure, function and synthesis of cell surface polysaccharides in Escherichia coli, p. 437-494. In C. L Gyles (ed.), Escherichia coli in domestic animals and man. CAB International, Wallingford, Oxon, United Kingdom.
54. Whitfield, C., G. Schoenhals, and L. Graham. 1989. Mutants of Escherichia coli O9:K30 with altered synthesis and expression of the capsular K antigen J. Gen. Microbiol. 135:2589-2599.
55. Whitfield, C., and M. A. Valvano. 1993. Biosynthesis and expression of cell surface polysaccharides in gram-negative bacteria. Adv. Microb Physiol 35: 135-246.
56. Zhang, L., A. Al-Hendy, P. Toivanen, and M. Skurnik. 1993. Genetic organization and sequence of the rfb gene cluster of Yersinia enterocolitica serotype O.3: similarities to the dTDP-L-rhamnose biosynthesis pathway of Salmonella and to the bacterial polysaccharide transport systems. Mol Microbiol. 9:309-321.