Duplicated Clostridium thermocellum cellobiohydrolase gene encoding cellulosomal subunits S3 and S5

Applied Microbiology and Biotechnology

Volumn 51, Issue 6, 1999, Pages 852-859

  Zverlov, V V ^a   Velikodvorskaya, G A ^a   Schwarz, W H ^b   Kellermann, J ^c   Staudenbauer, W L ^b  

^a INSTITUTE OF MOLECULAR GENETICS   (Russian Federation)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULASE; CELLULOSE 1,4 BETA CELLOBIOSIDASE; HYDROLASE; OLIGOSACCHARIDE; PROTEIN SUBUNIT;

AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CELLULAR DISTRIBUTION; CLOSTRIDIUM THERMOCELLUM; DNA FLANKING REGION; ENZYME ACTIVATION; ENZYME ACTIVITY; GENE EXPRESSION; GENE SEQUENCE; GENE STRUCTURE; MOLECULAR CLONING; OPERON; POLYMERASE CHAIN REACTION; STRUCTURE ACTIVITY RELATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CATALYTIC DOMAIN; CELLULASE; CELLULOSE 1,4-BETA-CELLOBIOSIDASE; CLONING, MOLECULAR; CLOSTRIDIUM; GENES, BACTERIAL; GENES, DUPLICATE; MOLECULAR SEQUENCE DATA; PHYSICAL CHROMOSOME MAPPING; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

EID: 0033014875     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051473     Document Type: Article

References (50)

1. Bayer EA, Morag E, Lamed R (1994) The cellulosome - A treasure-trove for biotechnology. Trends Biotechnol 12: 379-386
2. Béguin P, Lemaire M (1996) The cellulosome: an exocellular, multiprotein complex specialized in cellulose degradation. Crit Rev Biochem Mol Biol 31: 201-236
3. Berger E, Jones WA, Jones DT, Woods DR (1990) Sequencing and expression of a cellodextrinase (ced1) gene from Butyrivibrio fibrisolvens H17c cloned in Escherichia coli. Mol Gen Genet 223: 310-318
4. Bhat S, Goodenough PW, Bhat MK, Own E (1994). Isolation of four major subunits from Clostridium thermocellum cellulosome and their synergism in the hydrolysis of crystalline cellulose. Int J Biol Macromol 16: 335-342
5. Broussolle V, Forano E, Gaudet G, Ribot Y (1994) Gene sequence and analysis of protein domains of EGB, a novel family E endoglucanase from Fibrobacter succinogenes S58. FEMS Microbiol Lett 124: 439-447
6. Chauvaux S, Béguin P, Aubert J-P (1992) Site-directed mutagenesis of essential carboxylic residues in Clostridium thermocellum endoglucanase CelD. J Biol Chem 267: 4472-4478
7. Choi SK, Ljungdahl LG (1996) Dissociation of the cellulosome of Clostridium thermocellum in the presence of ethylenediamintetraacetic acid occurs with the formation of truncated peptides. Biochemistry 35: 4897-4905
8. Cornet P, Millet J, Béguin P, Aubert J-P (1983) Characterization of two cel (cellulose degradation) genes of Clostridium thermocellum coding for endoglucanases. Biotechnology 1: 589-594
9. Coutinho JB, Moser B, Kilburn DG, Warren RAJ, Miller RC (1991) Nucleotide sequence of the endoglucanase C gene (cenC) of Cellulomonas fimi, its high-level expression in Escherichia coli, and characterization of its products. Mol Microbiol 5: 1221-1233
10. Davies GJ, Tolley SP, Henrissat B, Hjort C, Schülein M (1995) Structures of oligosaccharide-bound forms of the endoglucanase V from Humicola insolens at 1.9 A resolution. Biochemistry 34: 16 210-16 220
11. Divne C, Stahlberg J, Reinikainen T, Ruohonen L, Pettersson G, Knowles JKC, Teeri TT, Jones TA (1994) The three-dimensional crystal structure of the catalytic core of cellobiohydrolase I from Trichoderma reesei. Science 265: 524-528
12. Felix CR, Ljungdahl LG (1993) The cellulosome: the exocellular organelle of Clostridium. Annu Rev Microbiol 47: 791-819
13. Fujino T, Béguin P, Aubert J-P (1993) Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal protein CipA and a protein possibly involved in attachment of the cellulosome to the cell surface. J Bacteriol 175: 1891-1899
14. Garcia-Campayo V, Béguin P (1997) Synergism between the cellulosome-integrating protein CipA and endoglucanase CelD of Clostridium thermocellum. J Biotechnol 57: 39-47
15. L-protein reveals an unusual degree of internal homology. Mol Microbiol 8: 325-334
16. Guglielmi G, Béguin P (1998) Cellulase and hemicellulase genes of Clostridium thermocellum from five independent collections contain few overlaps and are widely scattered across the chromosome. FEMS Microbiol Lett 161: 209-215
17. Haldenwang WG (1995) The sigma factors of Bacillus subtilis. Microbiol Rev 59: 1-30
18. Hall J, Gilbert HJ (1988) The nucleotide sequence of a carboxy-methylcellulase gene from Pseudomonas fluorescens subsp. cellulosa. Mol Gen Genet 213: 112-117
19. Johnson EA, Madia A, Demain AL (1981) Chemically defined minimal medium for growth of the anaerobic cellulolytic thermophile Clostridium thermocellum. Appl Environ Microbiol 41: 1060-1062
20. Johnson PE, Tomme P, Joshi MD, McIntosh LP (1996) Interaction of soluble cellooligosaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 2. NMR and ultraviolet absorption spectroscopy. Biochemistry 35: 13 895-13 906
21. Joliff G, Béguin P, Juy M, Millet J, Ryter A, Poljak R, Aubert J-P (1986a) Isolation, crystallization and properties of a new cellulase of Clostridium thermocellum overproduced in Escherichia coli. Biotechnology 4: 896-900
22. Joliff G, Béguin P, Aubert JP (1986b) Nucleotide sequence of the cellulase gene celD encoding endoglucanase D of Clostridium thermocellum. Nucleic Acids Res 14: 8605-8613
23. Jung ED, Lao G, Irwin D, Barr BK, Benjamin A, Wilson DB (1993) DNA sequences and expression in Streptomyces lividans of an exoglucanase gene and an endoglucanase gene from Thermomonospora fusca. Appl Environ Microbiol 59: 3032-3043
24. Juy M, Amit AG, Alzari PM, Poljak RJ, Claeyssens M, Béguin P, Aubert J-P (1992) Three-dimensional structure of a thermos-table bacterial cellulase. Nature 375: 89-91
25. Kataeva IA, Li X-L, Chen HZ, Choi S-K, Ljungdahl LG (1997a) GenBank, accession number AF03930
26. Kataeva I, Guglielmi G, Béguin P (1997b) Interaction between Clostridium thermocellum endoglucanase CelD and polypeptides derived from the cellulosome-integrating protein CipA: stoichiometry and cellulolytic activity of the complexes. Biochem J 326: 617-624
27. Kruus K, Lua AC, Demain AL, Wu JHD (1995a) The anchorage function of CipA (CelL), a scaffolding protein of the Clostridium thermocellum cellulosome. Proc Natl Acad Sci USA 92: 9254-9258
28. Kruus K, Wang WK, Ching J, Wu JHD (1995b) Exoglucanase activities of the recombinant Clostridium thermocellum CelS, a major cellulosome component. J Bacteriol 177: 1641-1644
29. Leibovitz E, Béguin P (1996) A new type of cohesin domain that specifically binds the dockerin domain of the Clostridium thermocellum cellulosome-integrating protein CipA. J Bacteriol 178: 3077-3084
30. Malburg LM, Iyo AH, Forsberg CW (1996) A novel family 9 endoglucanase gene (celD), whose product cleaves substrates mainly to glucose, and its adjacent upstream homolog (celE) from Fibrobacter succinogenes S85. Appl Environ Microbiol 62: 898-906
31. Mel'nik MS, Rabinovich ML, Voznyi IV (1991) Cellobiohydrolase of Clostridium thermocellum, synthesized by a recombinant E. coli strain. Biokhimiya 56: 1787-1797
32. Morag E, Bayer EA, Lamed R (1991) Anomalous dissociative behavior of the major glycosylated component of the cellulosome of Clostridium thermocellum. Appl Biochem Biotechnol 30: 129-136
33. Morag E, Bayer EA, Lamed R (1992) Affinity digestion for the near-total recovery of purified cellulosome from Clostridium thermocellum. Enzyme Microb Technol 14: 289-292
34. S (CelS) is synonymous with the major cello-biohydrolase (subunit S8) from the cellulosome of Clostridium thermocellum
35. Morag E, Lapidot A, Govorko D, Lamed R, Wilchek M, Bayer EA, Shoham Y (1995) Expression, purification, and characterization of the cellulose-binding domain of the scaffoldin subunit from the cellulosome of Clostridium thermocellum. Appl Environ Microbiol 61: 1980-1986
36. Rouvinen J, Bergfors T, Teeri T, Knowles JKC, Jones TA (1990) Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei. Science 249: 380-386
37. Sakon J, Irwin D, Wilson DB, Karplus PA (1997) Structure and mechanism of endo/exocellulase E4 from Thermomonospora fusca. Nat Struct Biol 4: 810-818
38. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
39. Schlochtermeier A, Walter S, Schröder J, Moorman M, Schrempf H (1992) The gene encoding the cellulase (Avicelase) Cel1 from Streptomyces reticuli and analysis of protein domains. Mol Microbiol 6: 3611-3621
40. Shimon LJW, Bayer EA, Morag E, Lamed R, Yaron S, Shoham Y, Frolow F (1997) A cohesin domain from Clostridium thermocellum: the crystal structure provides new insights into cellulosome assembly. Structure 5: 381-390
41. Singh RN, Akimenko VK (1993) Isolation of a cellobiohydrolase of Clostridium thermocellum capable of degrading natural crystalline substrates. Biochem Biophys Res Commun 192: 1123-1130
42. Teeri TT (1997) Crystalline cellulose degradation: new insight into the function of cellobiohydrolases. Trends Biotechnol 15: 160-167
43. Tomme P, Warren RAJ, Gilkes NR (1995) Cellulose hydrolysis by bacteria and fungi. Adv Microb Physiol 37: 1-81
44. Tomme P, Creagh L, Kilburn D, Haynes C (1996a) Interaction of polysaccharides with the N-terminal cellulose-binding domain of Cellulomonas fimi CenC. 1. Binding specificity and calorimetric analysis. Biochemistry 35: 13 885-13 894
45. Tomme P, Kwan E, Gilkes NR, Kilburn DG, Warren RAJ (1996b) Characterization of CenC, an enzyme from Cellulomonas fimi with both endo- and exoglucanase activities. J Bacteriol 178: 4216-4223
46. Tormo J, Lamed R, Chirino AJ, Morag E, Bayer EA, Shoham Y, Steitz TA (1996) Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. EMBO J 5: 5739-5751
47. Tuka K, Zverlov VV, Bumazkin BK, Velikodvorskaya GA, Strongin AY (1990) Cloning and expression of Clostridium thermocellum genes coding for thermostable exoglucanases (cellobiohydrolases) in Escherichia coli cells. Biochem Biophys Res Commun 169: 1055-1060
48. Young M, Minton NP, Staudenbauer WL (1989) Recent advances in the genetics of clostridia. FEMS Microbiol Rev 63: 301-326
49. Zverlov VV, Velikodvorskaya GA, Schwarz WH, Bronnenmeier K, Kellermann J, Staudenbauer WL (1998a) Multidomain structure and cellulsomal localization of the Clostridium thermocellum cellobiohydrolase CbhA. J Bacteriol 180: 3091-3099
50. Zverlov V, Mahr S, Riedel K, Bronnenmeier K (1998b) Properties and gene structure of a bifunctional cellulolytic enzyme (CelA) from the extreme thermophile 'Anaerocellum thermophilum' with separate glycosyl hydrolase family 9 and 48 catalytic domains. Microbiology 144: 457-465