Cellulase and hemicellulase genes of Clostridium thermocellum from five independent collections contain few overlaps and are widely scattered across the chromosome

FEMS Microbiology Letters

Volumn 161, Issue 1, 1998, Pages 209-215

  Guglielmi, Gérard ^a   Béguin, Pierre ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

Cellulase gene; Clostridium thermocellum; Hemicellulase gene; Mapping

Indexed keywords

BACTERIAL DNA; BACTERIAL ENZYME; CELLULASE;

ARTICLE; CLOSTRIDIUM; CLOSTRIDIUM THERMOCELLUM; DNA PROBE; ENZYME SYNTHESIS; GENE MAPPING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; SOUTHERN BLOTTING;

CELLULASE; CHROMOSOME MAPPING; CLOSTRIDIUM; GENES, BACTERIAL; GLYCOSIDE HYDROLASES;

EID: 0032053815     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(98)00076-7     Document Type: Article

References (29)

1. Hazlewood, G.P., Romaniec, M.P.M., Davidson, K., Grépinet, O., Béguin, P., Millet, J., Raynaud, O. and Aubert, J.-P. (1988) A catalogue of Clostridium thermocellum endoglucanase, β-glucosidase and xylanase genes cloned in Escherichia coli. FEMS Microbiol. Lett. 51, 231-236.
2. Piruzian, E.S., Mogutov, M.A., Velikodvorskaya, G.A. and Akimenko, V.K. (1985) Cloning and expression of structural genes of Clostridium thermocellum F7 cellulolytic complex endoglucanases in cells of Escherichia coli K12. Dokl. Acad. Nauk. SSR 281, 963-965.
3. Schwarz, W., Bronnenmeier, K. and Staudenbauer, W.L. (1985) Molecular cloning of Clostridium thermocellum genes involved in β-glucan degradation in bacteriophage lambda. Biotechnol. Lett. 7, 859-864.
4. Sakka, K., Furuse, S. and Shimada, K. (1989) Cloning and expression in Escherichia coli of the thermophilic Clostridium sp. F1 genes related to cellulose hydrolysis. Agric. Biol. Chem. 53, 905-911.
5. Zverlov, V.V. and Velikodvorskaya, G.A. (1990) Cloning the Clostridium thermocellum thermostable laminarinase gene in Escherichia coli - The properties of the enzyme thus produced. Biotechnol. Lett. 12, 811-816.
6. L-protein reveals an unusual degree of internal homology. Mol. Microbiol. 8, 325-334.
7. Wang, W.K., Kruus, K. and Wu, J.H.D. (1994) Cloning and expression of the Clostridium thermocellum celS gene in Escherichia coli. Appl. Microbiol. Biotechnol. 42, 346-352.
8. Lemaire, M., Miras, I., Gounon, P. and Béguin, P. (1998) Identification of a region responsible for binding to the cell wall within the S-layer protein of Clostridium thermocellum. Microbiology 144, 211-217.
9. Fujino, T., Béguin, P. and Aubert, J.-P. (1993) Organization of a Clostridium thermocellum gene cluster encoding the cellulosomal scaffolding protein CipA and a protein possibly involved in the attachment of the cellulosome to the cell surface. J. Bacteriol. 175, 1891-1899.
10. Leibovitz, E. and Béguin, P. (1996) A new type of cohesin domain that specifically binds the dockerin domain of the Clostridium thermocellum cellulosome-integrating protein CipA. J. Bacteriol. 178, 3077-3084.
11. Hall, J., Hazlewood, G.P., Barker, P.J. and Gilbert, H.J. (1988) Conserved reiterated domains in Clostridium thermocellum endoglucanases are not essential for activity. Gene 69, 29-38.
12. Ahsan, M.M., Kimura, T., Karita, S., Sakka, K. and Ohmiya, K. (1996) Cloning, DNA sequencing, and expression of the gene encoding Clostridium thermocellum cellulase CelJ, the largest catalytic component of the cellulosome. J. Bacteriol. 178, 5732-5740.
13. Schimming, S., Schwarz, W.H. and Staudenbauer, W.L. (1992) Structure of the Clostridium thermocellum Gene licB and the encoded β-1,3-1,4-glucanase - A catalytic region homologous to Bacillus lichenases joined to the reiterated domain of clostridial cellulases. Eur. J. Biochem. 204, 13-19.
14. Schwarz, W.H., Schimming, S. and Staudenbauer, W.L. (1988) Isolation of a Clostridium thermocellum gene encoding a thermostable β-1,3-glucanase (laminarinase). Biotechnol. Lett. 10, 225-230.
15. Schwarz, W.H., Schimming, S., Rücknagel, K.P., Burgschwaiger, S., Kreil, G. and Staudenbauer, W.L. (1988) Nucleotide sequence of the celC gene encoding endoglucanase C of Clostridium thermocellum. Gene 63, 23-30.
16. Béguin, P., Rocancourt, M., Chebrou, M.-C. and Aubert, J.-P. (1986) Mapping of mRNA encoding endoglucanase A from Clostridium thermocellum. Mol. Gen. Genet. 202, 251-254.
17. Mishra, S., Béguin, P. and Aubert, J.-P. (1991) Transcription of Clostridium thermocellum endoglucanase genes celF and celD. J. Bacteriol. 173, 80-85.
18. Bélaïch, J.-P., Gaudin, C., Bélaïch, A., Bagnara-Tardif, C., Fierobe, H.-P. and Reverbel, C. (1993) Molecular organization and biochemistry of the cellulolytic system of Clostridium cellulolyticum. In: Proceedings of the Mie Bioforum 93 - Genetics, Biochemistry and Ecology of Lignocellulose Degradation (Shimada, K., Ohmiya, K., Kobayashi, Y., Hoshino, S., Sakka, K. and Karita, S., Eds.), pp. 53-62. UNI Publishers, Tokyo.
19. Fujino, T., Karita, S. and Ohmiya, K. (1993) Nucleotide sequences of the celB gene encoding endo-1,4-β-glucanase-2, ORF1 and ORF2 forming a putative cellulase gene cluster of Clostridium josui. J. Ferment. Bioeng. 76, 243-250.
20. Sakka, K., Shimanuki, T. and Shimada, K. (1991) Nucleotide sequence of celC307 encoding endoglucanase CelC307 of Clostridium sp. strain F-1. Agric. Biol. Chem. 55, 347-350.
21. Zverlov, V.V., Laptev, D.A., Tishkov, V.I. and Velikodvorskaya, G.A. (1991) Nucleotide sequence of the Clostridium thermocellum laminarinase gene. Biochem. Biophys. Res. Commun. 181, 507-512.
22. Joliff, G., Béguin, P., Juy, M., Millet, J., Ryter, A., Poljak, R. and Aubert, J.-P. (1986) Isolation, crystallization and properties of a new cellulase of Clostridium thermocellum overproduced in Escherichia coli. BioTechnology 4, 896-900.
23. Yagüe, E., Béguin, P. and Aubert, J.-P. (1990) Nucleotide sequence and deletion analysis of the cellulase-encoding gene celH of Clostridium thermocellum. Gene 89, 61-67.
24. Millet, J., Pétré, D., Béguin, P., Raynaud, O. and Aubert, J.-P. (1985) Cloning of ten distinct DNA fragments of Clostridium thermocellum coding for cellulases. FEMS Microbiol. Lett. 29, 145-149.
25. Salamitou, S., Lemaire, M., Fujino, T., Ohayon, H., Gounon, P., Béguin, P. and Aubert, J.-P. (1994) Subcellular localization of Clostridium thermocellum ORF3p, a protein carrying a receptor for the docking sequence borne by the catalytic components of the cellulosome. J. Bacteriol. 176, 2828-2834.
26. Kataeva, I., Guglielmi, G. and Béguin, P. (1997) Interaction between Clostridium thermocellum endoglucanase CelD and polypeptides derived from the cellulosome-integrating protein CipA: stoichiometry and cellulolytic activity of the complexes. Biochem. J. 326, 617-624.
27. Gräbnitz, F. and Staudenbauer, W.L. (1988) Characterization of two β-glucosidase genes from Clostridium thermocellum. Biotechnol. Lett. 10, 73-78.
28. Tuka, K., Zverlov, V.V., Bumazkin, B.K., Velikodvorskaya, G.A. and Strongin, A.Y. (1990) Cloning and expression of Clostridium thermocellum genes coding for thermostable exoglucanases (cellobiohydrolases) in Escherichia coli cells. Biochem. Biophys. Res. Commun. 169, 1055-1060.
29. Bumazkin, B.K., Velikodvorskaya, G.A., Tuka, K., Mogutov, M.A. and Strongin, A.Y. (1990) Cloning of Clostridium thermocellum endoglucanase genes in Escherichia coli. Biochem. Biophys. Res. Commun. 167, 1057-1064.