DEAD-box protein HEL64 from Trypanosoma brucei: Subcellular localization and gene knockout analysis

Parasitology Research

Volumn 85, Issue 4, 1999, Pages 324-330

  Missel, Andreas ^b   Lambert, Laurence ^a   Nörskau, Gesa ^a   Göringer, H Ulrich ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b QIAGEN GMBH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELLULAR DISTRIBUTION; GENE LOCUS; HOST PARASITE INTERACTION; INSECT; KNOCKOUT GENE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RNA METABOLISM; TRYPANOSOMA BRUCEI;

ALLELES; ANIMALS; ANTIBODIES, PROTOZOAN; BLOTTING, SOUTHERN; BLOTTING, WESTERN; CENTRIFUGATION, DENSITY GRADIENT; CROSS REACTIONS; EUKARYOTIC INITIATION FACTOR-4A; GENE TARGETING; GENES, PROTOZOAN; MUTATION; PEPTIDE INITIATION FACTORS; PLASMIDS; RNA HELICASES; TRANSFECTION; TRYPANOSOMA BRUCEI BRUCEI;

ANIMALIA; INSECTA; PROTOZOA; TRYPANOSOMA; TRYPANOSOMA BRUCEI; TRYPANOSOMA BRUCEI BRUCEI;

EID: 0032981681     PISSN: 00443255     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004360050556     Document Type: Article

References (42)

1. Alfonzo JD, Thiemann O, Simpson L (1997) The mechanism of U insertion/deletion RNA editing in kinetoplastid mitochondria. Nucleic Acids Res 25: 3751-3759
2. Beverley SM, Clayton CE (1993) Transfection of Leishmania and Trypanosoma brucei by electroporation. In: Hyde JE (ed) Protocols in molecular parasitology. Humana, Totowa, New Jersey, pp 333-348
3. Biebinger S, Lorenz P, Wirtz LE, Clayton CE (1997) Vectors for inducible expression of toxic gene products in bloodstream and procyclic Trypanosoma brucei. Mol Biochem Parasitol 85:99-112
4. Blundell PA, Rudenko G, Borst P (1996) Targeting of exogenous DNA into Trypanosoma brucei requires a high degree of homology between donor and target DNA. Mol Biochem Parasitol 76: 215-229
5. Bringaud F, Peyruchaud S, Baltz D, Giroud C, Simpson L, Baltz T (1995) Molecular characterization of the mitochondrial heat shock protein 60 gene from Trvpanosoma brucei. Mol Biochem Parasitol 74: 119-123
6. Brun R, Schönenberger M (1979) Cultivation and in vitro cloning of procyclic forms of Trypanosoma brucei in a semi-defined medium. Acta Trop (Basel) 36: 289-292
7. Clayton CE, Michels P (1996) Metabolic compartmentation in African trypanosomes. Parasitol Today 12: 465-471
8. Cross M, Günzl A, Palfi Z, Bindereif A (1991) Analysis of small nuclear ribonucleoproteins (RNPs) in Trypanosoma brucei - structural organization and protein-components of the spliced leader RNP. Mol Cell Biol 11: 5516-5526
9. Cruz A, Beverley SM (1990) Gene replacement in parasitic protozoa. Nature 348: 171-173
10. Dorn R, Morawietz H, Reuter G, Saumweber H (1993) Identification of an essential Drosophila gene that is homologous to the translation initiation factor eIF-4A of yeast and mouse. Mol Gen Genet 237: 233-240
11. Dumas C, Oullette M, Tavor J, Cunningham ML, Fairlamb AH, Tamar S, Olivier M, Papadopoulou B (1997) Disruption of the trypanothione reductase gene of Leishmania decreases its ability to survive oxidative stress in macrophages. EMBO J 16: 2590-2598
12. Gaud A, Carrinton M, Deshusses J, Schaller DR (1997) Polymerase chain reaction-based gene disruption in Trypanosoma brucei. Mol Biochem Parasitol 87: 113-115
13. Göringer HU, Koslowsky DJ, Morales TH, Stuart K (1994) The formation of mitochondrial ribonucleoprotein complexes involving guide RNA molecules in Trypanosoma brucei. Proc Natl Acad Sci USA 91: 1776-1780
14. Harlow E, Lane D (1988) Antibodies - a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
15. Harris ME, Moore DR, Hajduk SL (1990) Addition of uridines to edited RNAs in trypanosome mitochondria occurs independently of transcription. J Biol Chem 265: 11368-11376
16. Hehl A, Pearson TW, Barry JD, Braun R, Roditi I (1995) Expression of GARP, a major surface glycoprotein of Trypanosoma congolense, on the surface of Trypanosoma brucei: characterization and use as a selectable marker. Mol Biochem Parasitol 70: 45-58
17. Hochuli E (1990) Purification of recombinant proteins with metal chelate adsorbent. Genet Eng 12: 87-98
18. Hunger-Glaser I, Seebeck T (1997) Deletion of the genes for the paraflagellar rod protein PFR-A in Trypanosoma brucei is probably lethal. Mol Biochem Parasitol 90: 347-351
19. Iggo RD, Lane DP (1989) Nuclear protein p68 is an RNA-dependent ATPase. EMBO J 8: 1827-1831
20. Iggo RD, Jamieson DJ, MacNeill SA, McPheat JS, Lane DP (1991) p68 RNA helicase: identification of a nucleolar form and cloning of related genes containing a conserved intron in yeasts. Mol Cell Biol 11: 1326-1333
21. Kable ML, Heidmann S, Stuart KD (1997) RNA editing: getting U into RNA. TIBS 22: 162-166
22. Lane DP, Hoeffler WK (1980) SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68000. Nature 288: 167-170
23. Lee MGS, Ploeg LHT van der (1990) Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei. Science 250: 1583-1587
24. Linder P, Slommski PP (1989) An essential yeast protein, encoded by duplicated genes TIF1 and TIF2 and homologous to the mammalian translation initiation factor eIF-4A, can suppress a mitochondrial missense mutation. Proc Natl Acad Sci USA 86: 2286-2290
25. Maizels RM, Blaxter ML, Robertson BD, Selkirk ME (1991) Parasite antigens, parasite genes - a laboratory manual for molecular parasitology. Press Syndicate, University of Cambridge, Cambridge
26. Manivasakam P, Weber SC, McElver J, Schiestl RH (1995) Microhomology mediated PCR targeting in Saccharomyces cerevisiae. Nucleic Acids Res 23 2799-2800
27. Marzluff WF (1990) Preparation of active nuclei. Methods Enzymol 181: 300-306
28. Missel A, Nörskau G, Shu HH, Göringer HU (1995) A putative RNA helicase of the DEAD box family from Trypanosoma brucei. Mol Biochem Parasitol 75: 123-126
29. Missel A, Souza AE, Nörskau G, Göringer HU (1997) Disruption of a gene encoding a novel mitochondrial DEAD-box protein in Trypanosoma brucei affects edited mRNAs. Mol Cell Biol 17: 4895-4903
30. Papadopoulou B, Dumas C (1997) Parameters controlling the rate of gene targeting frequency in the protozoan parasite Leishmania. Nucleic Acids Res 25: 4278-4286
31. Pause A, Sonenberg N (1992) Mutational analysis of a DEAD box RNA helicase. the mammalian translation initiation factor eIF-4A. EMBO J 11: 2643-2654
32. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
33. Schimmang T, Tollervey D, Kern H, Frank R, Hurt EC (1989) A yeast nucleolar protein related to mammalian fibrillarin is associated with small nucleolar RNA and is essential for viability. EMBO J 8: 4015-4024
34. Schmid SR, Linder P (1992) D-E-A-D protein family of putative RNA helicases. Mol Microbiol 6: 283-291
35. Siomi IL, Dreyfuss G (1997) RNA-binding proteins as regulators of gene expression. Curr Opin Genet Dev 7: 345-353
36. Sloof P, Benne R (1997) RNA editing in kinetoplastid parasites: what to do with U. Trends Microbiol 5: 189-195
37. Stuart K, Gobright E, Jenni L, Milhausen LM, Thomashow L, Agabian N (1984) The IsTaR 1 serodeme of Trypanosoma brucei: development of a new serodeme. J Parasitol 70: 747-754
38. Swindle J, Tait A (1996) Trypanosomatid genetics. In. Smith DF, Parsons M (eds) Molecular biology of parasitic protozoa. IRL Oxford, pp 6-34
39. Ten Asbroek ALMA, Mol CAAM, Kieft R, Borst P (1993) Stable transformation of Trypanosoma brucei. Mol Biochem Parasitol 59 133-142
40. Ullu E, Tschudi C, Günzl A (1996) trans-Splicing in trypanosomatid protozoa In: Smith DF, Parsons M (eds) Molecular biology of parasitic protozoa. IRL, Oxford, pp 115-133
41. Wirtz E, Hartmann C, Clayton CE (1994) Gene expression mediated by bacteriophage T3 and T7 RNA polymerases in transgenic trypanosomes, Nucleic Acids Res 19: 3887-3894
42. Woods A, Sherwin T, Sasse R, MacRae T, Baines AJ, Gull K (1989) Definition of individual components within the cytoskeleton of Trypanosoma brucei by a library of monoclonal antibodies J Cell Sci 93 491-500