Simultaneous monitoring of cell number and metabolic activity of specific bacterial populations with a dual gfp-luxAB marker system

Applied and Environmental Microbiology

Volumn 65, Issue 2, 1999, Pages 813-821

  Unge, Annika ^a   Tombolini, Riccardo ^a   Mølbak, Lars ^a   Jansson, Janet K ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN; LUCIFERASE;

ARTICLE; BACTERIAL COUNT; BACTERIAL METABOLISM; BACTERIUM TRANSFORMATION; BIOLUMINESCENCE; DIAGNOSTIC ACCURACY; ESCHERICHIA COLI; FLOW CYTOMETRY; LUMINOMETRY; NONHUMAN; PHENOTYPE; POPULATION DENSITY; PSEUDOMONAS FLUORESCENS; QUANTITATIVE DIAGNOSIS; SOIL MICROFLORA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS;

EID: 0032976010     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.2.813-821.1999     Document Type: Article

References (32)

1. Bailey, M. J., A. K. Lilley, I. P. Thompson, P. B. Rainey, and R. J. Ellis. 1995. Site directed chromosomal marking of a fluorescent pseudomonad isolated from the phytosphere of sugar beet; stability and potential for marker gene transfer. Mol. Ecol. 4:755-763.
2. Bakken, L. R., and V. Lindahl. 1995. Recovery of bacteria cells from soil, p. 9-27. In J. T. Trevors and J. D. van Elsas (ed.). Nucleic acids in the environment: methods and applications. Springer-Verlag, Heidelberg, Germany.
3. Bloemberg, G. V., G. A. O'Toole, B. J. J. Lugtenberg, and R. Kolter. 1997. Green fluorescent protein as a marker for Pseudomonas spp. Appl. Environ. Microbiol. 63:4543-4551.
4. Bokman, S. H., and W. W. Ward. 1981. Renaturation of Aequorea green-fluorescent protein. Biochem. Biophys. Res. Commun. 101:1372-1380.
5. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
6. Duncan, S., L. A. Glover, K. Killham, and J. I. Prosser. 1994. Luminescence-based detection of activity of starved and viable but nonculturable bacteria. Appl. Environ. Microbiol. 60:1308-1316.
7. Elhai, J. 1993. Strong and regulated promoters in the cyanobacterium Anabaena PCC 7120. FEMS Microbiol. Lett. 114:179-184.
8. Ferguson, Y., L. A. Glover, D. M. McGillivray, and J. I. Prosser. 1995. Survival and activity of lux-marked Aeromonas salmonicida in seawater. Appl. Environ. Microbiol. 61:3494-3498.
9. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
10. Heim, R., D. C. Prasher, and R. Y. Tsien. 1994. Wavelength mutations and posttranslational autoxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA 91:12501-12504.
11. Herrero, M., V. de Lorenzo, and K. Timmis. 1990. Transposon vectors containing non-antibiotic resistance markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J. Bacteriol. 172:6557-6567.
12. Hill, P. J., S. Swift, and G. S. A. B. Stewart. 1991. PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products. Mol. Gen. Genet. 226:41-48.
13. Holben, W. E., J. K. Jansson, B. K. Chelm, and J. M. Tiedje. 1988. Probe method for the detection of specific microorganisms in the soil bacterial community. Appl. Environ. Microbiol. 54:703-711.
14. Jansson, J. K. 1995. Tracking genetically engineered microorganisms in nature. Curr. Opin. Biotechnol. 6:275-283.
15. Jansson, J. K., and J. Prosser. 1997. Quantification of the presence and activity of specific microorganisms in nature. Mol. Biotechnol. 7:103-120.
16. Lindow, S. E. 1995. The use of reporter genes in the study of microbial ecology. Mol. Ecol. 4:555-566.
17. Meikle, A., K. Killham, J. I. Prosser, and L. A. Glover. 1992. Luminometric measurement of population activity of genetically modified Pseudomonas fluorescens in the soil. FEMS Microbiol. Lett. 99:217-220.
18. Meikle, A., L. A. Glover, K. Killham, and J. I. Prosser. 1994. Potential luminescence as an indicator of activation of genetically-modified Pseudomonas fluorescens in liquid culture and in soil. Soil Biol. Biochem. 26:747-755.
19. Möller, A., and J. K. Jansson. 1998. Detection of firefly luciferase-tagged bacteria in environmental samples. Methods Mol. Biol. 102:269-283.
20. Møller, S., C. Sternberg, J. B. Andersen, B. B. Christensen, J. L. Ramos, M. Givskov, and S. Molin. 1998. In situ gene expression in mixed-culture biofilms: evidence of metabolic interactions between community members. Appl. Environ. Microbiol. 64:721-732.
21. Ormö, M., A. B. Cubitt, K. Kallio, L. A. Gross, R. Y. Tsien, and S. J. Remington. 1996. Crystal structure of the Aequorea victoria green fluorescent protein. Science 273:1392-1395.
22. Porter, J., D. Deere, R. Pickup, and C. Edwards. 1996. Fluorescent probes and flow cytometry: new insights into environmental bacteriology. Cytometry 23:91-96.
23. Prosser, J. I. 1994. Molecular marker systems for the detection of genetically modified microorganisms in the environment. Microbiology 140:5-17.
24. Ropp, J. D., C. J. Donahue, D. Wolfgang-Kimball, J. J. Hooley, J. Y. W. Chin, R. A. Hoffman, R. A. Cuthbertson, and K. D. Bauer. 1995. Aequorea green fluorescent protein analysis by flow cytometry. Cytometry 21:309-317.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
26. Suarez, A., A. Güttler, M. Strätz, L. H. Staendener, K. N. Timmis, and C. A. Guzman. 1997. Green fluorescent protein-based reporter systems for genetic analysis of bacteria including monocopy applications. Gene 196:69-74.
27. Thompson, I. P., A. K. Lilley, R. J. Ellis, P. A. Bramwell, and M. J. Bailey. 1995. Survival, colonization and dispersal of genetically modified Pseudomonas fluorescens SBW25 in the phytosphere of field grown sugar beet. Bio/ Technology 13:1493-1497.
28. Tombolini, R., A. Unge, M. E. Davey, F. J. de Bruijn, and J. K. Jansson. 1997. Flow cytomctric and microscopic analysis of GFP-tagged Pseudomonas fluorescens bacteria. FEMS Microbiol. Ecol. 22:17-28.
29. Tombolini, R., and J. K. Jansson. 1998 Monitoring of GFP tagged bacterial cells. Methods Mol. Biol. 102:285-298.
30. Unge, A., R. Tombolini, A. Möller, and J. K. Jansson. 1997. Optimization of GFP as a marker for detection of bacteria in environmental samples, p. 391-394. In J. W. Hastings, L. J. Kricka, and P. E. Stanley (ed.). Bioluminescence and chemiluminescence: molecular reporting with photons. John Wiley & Sons, Chichester, United Kingdom.
31. Unge, A., R. Tombolini, M. E. Davey, F. J. de Bruijn, and J. K. Jansson. 1998. GFP as a marker gene. p. 6.1.13.1-6.1.13.16. In A. D. L. Akkermans, J. D. van Elsas, and F. J. de Bruijn (ed.). Molecular microbial ecology manual. Kluwer Academic Publishers, Dordrecht. The Netherlands.
32. Yang, F., L. G. Moss, and G. N. Phillips, Jr. 1996. The molecular structure of green fluorescent protein. Nat. Biotechnol. 14:1246-1251.