The DNA helicase activity of BLM is necessary for the correction of the genomic instability of Bloom syndrome cells

Molecular Biology of the Cell

Volumn 10, Issue 3, 1999, Pages 665-676

  Neff, Norma F ^a   Ellis, Nathan A ^b   Tian Zhang, Ye ^b   Noonan, James ^a   Huang, Kelly ^c   Sanz, Maureen ^c   Proytcheva, Maria ^d  

^a NEW YORK BLOOD CENTER   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^c WEILL CORNELL MEDICAL COLLEGE   (United States)

^d ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; HELICASE; PROTEIN BLM; UNCLASSIFIED DRUG;

ARTICLE; BLOOM SYNDROME; CELLULAR DISTRIBUTION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME LOCALIZATION; GENE MUTATION; GENETIC STABILITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0032964641     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.10.3.665     Document Type: Article

References (47)

1. Bennett, R., Sharp, J., and Wang, J.C. (1998). Purification and characterization of the Sgs1 DNA helicase activity. J. Biol. Chem. 273, 9644-9650.
2. Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254.
3. Davey, S., Han, C.S., Ramer, S.A., Klassen, J.C., Jacobson, A., Eisenberger, A., Hopkins, K.M., Lieberman, H.B., and Freyer, G.A. (1998). Fission yeast rad12+ regulates cell cycle checkpoint control and is homologous to the Bloom syndrome gene. Mol. Cell. Biol. 18, 2721-2728.
4. Ellis, N. (1997). DNA helicases in inherited human disorders. Curr. Opin. Gen. Dev. 7, 354-363.
5. Ellis, N.A., Groden, J., Ye, T.-Z., Straughen, J., Lennon, D.J., Ciocci, S., Proytcheva, M., and German, J. (1995a). The Bloom syndrome gene product is homologous to RecQ helicases. Cell 83, 655-666.
6. Ellis, N.A., Lennon, D.J., Proytcheva, M., Alhadeff, B., Henderson, E.E., and German, J. (1995b). Somatic intragenic recombination within the mutated locus BLM can correct the high sister-chromatid exchange phenotype of Bloom syndrome cells. Am. J. Hum. Genet. 57, 1019-1027.
7. Epstein, C.J., Martin, G.M., Schultz, A.L., and Motulsky, A.G. (1966). Werner syndrome. Medicine 45, 177-221.
8. Fukuchi, K.-I., Martin, G.M., and Monnat, R.J. (1989). Mutator phenotype of Werner syndrome is characterized by extensive deletions. Proc. Natl. Acad. Sci. USA 86, 5893-5897.
9. Galitski, T., and Roth, J.R. (1997). Pathways for homologous recombination between chromosomal direct repeats in Salmonella typhimurium. Genetics 146, 751-767.
10. Gangloff, S., McDonald, J. P., Bendixen, C., Arthur, L., and Rothstein, R. (1994). The yeast type I topoisomerase top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. Mol. Cell. Biol. 14, 8391-8398.
11. German, J. (1993). Bloom syndrome: a mendelian prototype of somatic mutational disease. Medicine 72, 393-406.
12. German, J., and Ellis, N.A. (1997). Bloom syndrome. In: Metabolic and Molecular Basis of Inherited Diseases, ed. C.R. Scriver, A.L. Beaudet, W.S. Sly, and D. Valle, New York: McGraw-Hill, 301-315.
13. Giesler, T., Baker, K., Zhang, B., McDaniel, L.D., and Schultz, R. (1997). Correction of the Bloom syndrome cellular phenotypes. Somatic Cell Mol. Genet. 23, 303-312.
14. Golemis, J., Gyuris, J., and Brent, R. (1998). Saccharomyces cerevisiae, Unit 13. In: Current Protocols in Molecular Biology, ed. F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl, New York: John Wiley & Sons, 13.1-13.13.
15. Goto, K., Akematsu, T., Shimazu, H., and Sugiyama, T. (1975). Simple differential staining of sister chromatids after treatment with photosensitive dyes and exposure to light and the mechanism of staining. Chromosoma 53, 223-230.
16. Gray, M.D., Shen, J.-C., Kamath-Loeb, A.S., Blank, A., Sopher, B.L., Martin, G.M., Oshima, J., and Loeb, L.A. (1997). The Werner syndrome protein is a DNA helicase. Nat. Genet. 17, 100-103.
17. Hanada, K., Ukita, T., Kohno, Y., Saito, K., Kato, J., and Ikeda, H. (1997). RecQ DNA helicase is a suppressor of illegitimate recombination in Escherichia coli. Proc. Natl. Acad. Sci. USA 94, 3860-3865.
18. Harlow, E., and Lane, D. (1988). Antibodies: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
19. Hoehn, H., Bryant, E.M., Au, K., Norwood, T.H., Boman, H., and Martin, G.M. (1975). Variegated translocation mosaicism in human skin fibroblasts. Cytogenet. Cell Genet. 15, 282-298.
20. Kaneko, H., et al. (1997). BLM (the causative gene of Bloom syndrome) protein translocation into the nucleus by a nuclear localization signal. Biochem. Biophys. Res. Commun. 240, 348-353.
21. Karow, J.K., Chakraverty, R.K., and Hickson, I.D. (1997). The Bloom syndrome gene product is a 3′ to 5′ DNA helicase. J. Biol. Chem. 272, 30611-30614.
22. Korf, B.R. (1997). Clinical cytogenetics, Unit 8, 5. In: Current Protocols in Human Genetics, ed. N.C. Dracopoli, J.L. Haines, B.R. Korf, D.T. Moir, C.C. Morton, C.E. Seidman, J.G. Seidman, and D.R. Smith, New York: John Wiley & Sons, 8.5.1-8.5.5.
23. Kunes, S., Ma, H., Overbye, K., Fox, M., and Botstein, D. (1987). Fine structure recombinational analysis of cloned genes using yeast transformation. Genetics 115, 73-84.
24. Kuzminov, A. (1995). Instability of inhibited replication forks in E. coli. Bioessays 17, 733-741.
25. Lohman, T.M., and Bjornson, K.P. (1996). Mechanisms of helicase-catalyzed DNA unwinding. Annu. Rev. Biochem. 65, 169-214.
26. Lu, J., Mullen, J.R., Brill, S.J., Kleff, S., Romeo, A.M., and Sternglanz, R. (1996). Human homologues of yeast helicase. Nature 383, 678-679.
27. Marciniak, R., Lombard, D., Johnson, F.B. and Guarente, L. (1998). Nucleolar localization of the Werner syndrome protein in human cells. Proc. Natl. Acad. Sci. USA 95, 6887-6892.
28. Matson, S.W., and Kaiser-Rogers, K.A. DNA helicases. (1990). Annu. Rev. Biochem. 59, 289-329.
29. Matsumoto, T., Shimamoto, A., Goto, M. and Furuichi, Y. (1997). Impaired nuclear localization of defective DNA helicases in Werner's syndrome. Nat. Genet. 16, 335-336.
30. Morozov, V., Mushegian, A.R., Koonin, E.V., and Bork, P. (1997). A putative nucleic acid-binding domain in Bloom and Werner syndrome helicases. Trends Biochem. Sci. 22, 417-418.
31. Mushegian, A.R., Bassett, Jr., D.E., Boguski, M.S., Bork, P., and Koonin, E.V. (1997). Positionally cloned human disease genes: patterns of evolutionary conservation and functional motifs. Proc. Natl. Acad. Sci. USA 94, 5831-5836.
32. Puranam, K.L., and Blackshear, P.J. (1994). Cloning and characterization of RECQL, a potential human homologue of the Escherichia coli DNA helicase RecQ. J. Biol. Chem. 269, 29838-29845.
33. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
34. Sanchez-Alonso, P., and Guzman, P. (1998). Organization of chromosome ends in Ustilago maydis. RecQ-like helicase motifs at telomeric ends. Genetics 148, 1043-1054.
35. Schachman, H.K., Adler, J., Radding, C.M., Lehman, I.R., and Kornberg, A. (1960). Enzymatic synthesis of DNA. VII. Synthesis of a polymer of deoxyadenylate and deoxythymidylate. J. Biol. Chem. 253, 3242-3249.
36. Seo, Y.-S., and Hurwitz, J. (1993). Isolation of helicase α, a DNA helicase from HeLa cells stimulated by a fork structure and single-stranded DNA-binding proteins. J. Biol. Chem. 268, 10282-10295.
37. Sherman, F. (1991). Getting started with yeast. Methods Enzymol. 194, 3-20.
38. Sinclair, D.A., and Guarente, L. (1997). Extrachromosomal rDNA circles: a cause of aging in yeast. Cell 91, 1033-1042.
39. Sinclair, D.A., Mills, K., and Guarente, L. (1997). Accelerated aging and nucleolar fragmentation in yeast sgs1 mutants. Science 277, 1313-1316.
40. +, a fission yeast gene related to the Bloom and Werner syndrome genes, is required for reversible S-phase arrest. EMBO J. 16, 2682-2692.
41. Studier, W., Rosenberg. A., Dunn, J., and Dubendorff, J. (1990). Use of T7 RNA polymerase to direct the expression of cloned genes. Methods Enzymol. 185, 60-89.
42. Thomas, B., and Rothstein, R. (1989). Elevated recombination rates in transcriptionally active DNA. Cell 56, 619-630.
43. Umezu, K., Nakayama, K., and Nakayama, H. (1984). Escherichia coli RecQ protein is a DNA helicase. Proc. Natl. Acad. Sci. USA 87, 5363-5367.
44. Vassilev, L.K., and Russev, G. (1984). Hydroxyurea treatment does not prevent initiation of DNA synthesis in Ehrlich ascites tumor cells and leads to the accumulation of short DNA fragments containing the replication origins. Biochim. Biophys. Acta 781, 39-44.
45. Watt, P.M., Louis, E.J., Borts, R.H., and Hickson, I.H. (1995). Sgs1: a eukaryotic homolog of E. coli RecQ that interacts with topoisomerase II in vivo and is required for faithful chromosome segregation. Cell 81, 253-260.
46. Watt, P.M., Hickson, I.D., Borts, R.H., and Louis, E.J. (1996). SGS1, a homologue of the Bloom and Werner's syndrome genes, is required for maintenance of genome stability in S. cerevisiae. Genetics 144, 935-945.
47. Yu, C.-E., et al. (1996). Positional cloning of the Werner's syndrome gene. Science 272, 258-262.