Accelerated aging and nucleolar fragmentation in yeast SGS1 mutants

Science

Volumn 277, Issue 5330, 1997, Pages 1313-1316

  Sinclair, David A ^a   Mills, Kevin ^a   Guarente, Leonard ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HELICASE;

ARTICLE; CELL AGING; FUNGAL GENETICS; FUNGUS MUTANT; GENE MUTATION; LIFESPAN; NONHUMAN; NUCLEOLUS; PRIORITY JOURNAL; SEQUENCE HOMOLOGY; SILENCER GENE; WERNER SYNDROME; YEAST;

CELL AGING; CELL DIVISION; CELL NUCLEOLUS; DNA HELICASES; FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT; FUNGAL PROTEINS; GENES, FUNGAL; HUMANS; MUTATION; PHENOTYPE; RECQ HELICASES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SILENT INFORMATION REGULATOR PROTEINS, SACCHAROMYCES CEREVISIAE; TRANS-ACTIVATORS; WERNER SYNDROME;

EID: 0030820491     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5330.1313     Document Type: Article

References (39)

1. N. A. Ellis et al., Cell 83, 655 (1995).
2. K. L. Puranam and P. J. Blackshear, J. Biol. Chem. 269, 29838 (1994); M. Seki et al., Nucleic Acids Res. 22, 4566 (1994).
3. K. L. Puranam and P. J. Blackshear, J. Biol. Chem. 269, 29838 (1994); M. Seki et al., Nucleic Acids Res. 22, 4566 (1994).
4. C.-E. Yu et al. Science 272, 258 (1996).
5. D. Salk, K. Au, H. Hoehn, M. R. Stenchever, G. M. Martin, Cytogenet. Cell Genet. 30, 108 (1981); G. M. Martin, Adv. Exp. Med. Biol. 190, 161 (1985).
6. D. Salk, K. Au, H. Hoehn, M. R. Stenchever, G. M. Martin, Cytogenet. Cell Genet. 30, 108 (1981); G. M. Martin, Adv. Exp. Med. Biol. 190, 161 (1985).
7. S. Gangloff, J. P. McDonald, C. Bendixen, L. Arthur, R. Rothstein, Mol. Cell. Biol. 14, 8391 (1994).
8. P. W. Watt, E. J. Louis, R. H. Borts, I. D. Hickson, Cell 81, 253 (1995).
9. P. M. Watt, I. Hickson, R. H. Borts, E. J. Louis, Genetics 144, 935 (1996).
10. R. K. Mortimer and J. R. Johnson, Nature 183, 1751 (1959).
11. I. Müller, J. Microbiol. Serol. 51, 1 (1985).
12. T. Smeal, J. Claus, B. Kennedy, F. Cole, L. Guarente, Cell 84, 633 (1996).
13. S. Kim, B. Villeponteau, S. M. Jazwinski, Biochim. Biophys. Res. Commun. 219, 370 (1996).
14. A. J. Klar, J. N. Strathern, J. R. Broach, J. B. Hicks, Nature 289, 239 (1981); J. M. Ivy, A. J. S. Klar, J. B. Hicks, Mol. Cell. Biol. 6, 688 (1986); J. Rine and I. Herskowitz, Genetics 116, 9 (1987).
15. A. J. Klar, J. N. Strathern, J. R. Broach, J. B. Hicks, Nature 289, 239 (1981); J. M. Ivy, A. J. S. Klar, J. B. Hicks, Mol. Cell. Biol. 6, 688 (1986); J. Rine and I. Herskowitz, Genetics 116, 9 (1987).
16. A. J. Klar, J. N. Strathern, J. R. Broach, J. B. Hicks, Nature 289, 239 (1981); J. M. Ivy, A. J. S. Klar, J. B. Hicks, Mol. Cell. Biol. 6, 688 (1986); J. Rine and I. Herskowitz, Genetics 116, 9 (1987).
17. D. E. Gottschling, O. M. Aparicio, B. L. Billington, V. Zakian, Cell 63, 751 (1990); O. M. Aparicio, B. L. Billington, D. E. Gottschling, ibid. 66, 1279 (1991).
18. D. E. Gottschling, O. M. Aparicio, B. L. Billington, V. Zakian, Cell 63, 751 (1990); O. M. Aparicio, B. L. Billington, D. E. Gottschling, ibid. 66, 1279 (1991).
19. M. Bryk et al., Genes Dev. 11, 255 (1997); J. S. Smith and J. D. Boeke, ibid., p. 241.
20. M. Bryk et al., Genes Dev. 11, 255 (1997); J. S. Smith and J. D. Boeke, ibid., p. 241.
21. S. Gottleib and R. E. Esposito, Cell 56, 771 (1989).
22. B. K. Kennedy et al., ibid. 89, 381 (1997).
23. B. K. Kennedy, N. R. Austriaco, J. Zhang, L. Guarente, ibid. 80, 485 (1995).
24. M. Gotta et al., EMBO J. 16, 3243 (1997).
25. D. S. McNabb, Y. Xing, L. Guarente, Genes Dev. 9, 47 (1995).
26. J. Horiuchi, N. Silverman, B. Piña, G. A. Marcus, L. Guarente, Mol. Cell. Biol. 17, 3220 (1997).
27. R. Henriquez, G. Blobel, J. P. Aris, J. Biol. Chem. 265, 2209 (1990).
28. D. Tollervey, H. Lehtonen, M. Carmo-Fonseca, E. C. Hurt, EMBO J. 10, 573 (1997).
29. F. Palladino et al., Cell 75, 543 (1993).
30. B. Strehler, Exp. Gerontol. 21, 283 (1986); J. Halle, S. Müller, A. Simm, G. Adam, Eur. J. Cell. Biol., in press.
31. B. Strehler, Exp. Gerontol. 21, 283 (1986); J. Halle, S. Müller, A. Simm, G. Adam, Eur. J. Cell. Biol., in press.
32. F. B. Adamstone and A. B. Taylor, J. Morphol. 154, 459 (1979); F. Puvion-Dutilleul and A. Macieira-Coelho, Exp. Cell Res. 138, 423 (1983); M. E. Weintein and A. B. Mukherjee, Mech. Ageing. Dev. 42, 215 (1988).
33. F. B. Adamstone and A. B. Taylor, J. Morphol. 154, 459 (1979); F. Puvion-Dutilleul and A. Macieira-Coelho, Exp. Cell Res. 138, 423 (1983); M. E. Weintein and A. B. Mukherjee, Mech. Ageing. Dev. 42, 215 (1988).
34. F. B. Adamstone and A. B. Taylor, J. Morphol. 154, 459 (1979); F. Puvion-Dutilleul and A. Macieira-Coelho, Exp. Cell Res. 138, 423 (1983); M. E. Weintein and A. B. Mukherjee, Mech. Ageing. Dev. 42, 215 (1988).
35. F. W. Studier, J. Mol. Biol. 189, 113 (1991).
36. P. K. Foreman and R. W. Davis, Gene 144, 63 (1994).
37. The region encoding the COOH-terminus of Sgs1 was amplified by the polymerase chain reaction (PCR) (from +3208 to +4344) with oligonucleotides GGGGGGGATCCAATTGTAGAAATAGCGCCAACG and GGGGGGAGCTCTCACTTTCTTCCTCTGTAGTGA. The product ligated to pET-28a(+) (Novagen) between Bam HI and Sac I to create pET28N-SGS1. BL21(DE3) cells (26) were transformed with pET28N-SGS1 and grown in 2 liters of complete medium to an absorbance at 600 nm of 1. Expression was induced with 1 mM isopropyl-β-D-thiogalactopyranoside for 4 hours. Sgs1p was purified with Ni-nitrilotriacetic acid (NTA) agarose under standard conditions.
38. SGS1 was amplified by the PCR with oligonucleotides GGGGGGGATCCAGTGACGAAGCCGTCACATAACTTA and GGGGGGCGGCCGCTTATCACTTTCTTCCTCTGTAGTGACC for insertion downstream of the galactose-inducible promoter of yCPIF15 (27). Overexpression of GALp-SGS1 was induced by growing for 4 hours in YPGal (10 g/liter yeast extract, 20 g/liter bactopeptone, 2 g/liter galactose), and confirmed by protein immunoblot analysis.
39. We thank S. Gasser and E. Hurt for antibodies to Sir3p and Nop1p; J. Broach (pDM42), J. Haber (pCW9-1), B. Johnson, D. McNabb, J. Horiuchi, and S. Mah for plasmids and advice; Y.-H. Tu for CELLScan imaging assistance (NIH S10RR917-01); and the Kaiser lab for their Axioscope. Supported by the Helen Hay Whitney Foundation (D.A.S.) and by a National Institutes of Health predoctoral training grant (K.M.). The Guarente lab is supported by a National Institutes of Health grant (AG11119).