Identification of an Escherichia coli pepA homolog and its involvement in suppression of the algB phenotype in mucoid Pseudomonas aeruginosa

Journal of Bacteriology

Volumn 181, Issue 1, 1999, Pages 107-116

  Woolwine, Samuel C ^a   Wozniak, Daniel J ^a  

^a WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALGINIC ACID; CYTOSOL AMINOPEPTIDASE; REGULATOR PROTEIN;

ARTICLE; GENE EXPRESSION; GENE ISOLATION; GENE MUTATION; GENE REPRESSION; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SEQUENCE HOMOLOGY; TRANSCRIPTION REGULATION;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0032955582     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.181.1.107-116.1999     Document Type: Article

References (54)

1. Altschul, S. F., T. L. Madden, A. A. Schäffer, J. Zhang, Z. Zhang, W. Miller, and D. L. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25:3389-3402.
2. Baynham, P. J., and D. J. Wozniak. 1996. Identification and characterization of AlgZ, an AlgT-dependent DNA binding protein required for Pseudomonas aeruginosa algD transcription. Mol. Microbiol. 22:97-108.
3. Berry, A., J. D. DeVault, and A. M. Chakrabarty. 1989. High osmolarity is a signal for enhanced algD transcription in mucoid and nonmucoid Pseudomonas aeruginosa strains. J. Bacteriol. 171:2312-2317.
4. Bjork, G. R., P. M. Wikstrom, and A. S. Bystrom. 1989. Prevention of translational frameshifting by the modified nucleosidc 1-methylguanosine. Science 244:986-989.
5. Blattner, F. R., G. Plunkett III, C. A. Bloch, N. T. Perna, V. Burland, M. Riley, J. Collado-Vides, J. D. Glasner, C. K. Rode, G. F. Mayhew, J. Gregor, N. W. Davis, H. A. Kirkpatrick, M. A. Goeden, D. J. Rose, B. Mau, and Y. Shao. 1997. The complete genome sequence of Escherichia coli K-12. Science 277:1453-1462.
6. Boucher, J. C., J. Martinez-Salazar, M. J. Schurr, M. H. Mudd, H. Yu, and V. Deretic. 1996. Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrA. J. Bacteriol. 178:511-523.
7. Boyer, H. W., and D. Roullard-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459-472.
8. Bukau, B. 1993. Regulation of the Escherichia coli heat-shock response. Mol. Microbiol. 9:671-680.
9. Burley, S. K., P. R. David, R. M. Sweet, A. Taylor, and W. N. Lipscomb. 1992. Structure determination and refinement of bovine lens leucine aminopeptidase and its complex with bestatin. J. Mol. Biol. 224:113-140.
10. Bystrom, A. S., K. J. Hjalmarsson, P. M. Wikstrom, and G. R. Bjork. 1983. The nucleotide sequence of an Escherichia coli operon containing genes for the tRNA (m1G) methyltransferase, the ribosomal proteins S16 and L19 and a 21-K polypeptide. EMBO J. 2:899-905.
11. χ subunit of DNA polymerase III holoenzyme of Escherichia coli. Mol. Gen. Genet. 241:399-408.
12. Charlier, D., D. Gigot, N. Huysveld, M. Rouvers, A. Piérard, and N. Glansdorff. 1995. Pyrimidine regulation of the Escherichia coli and Salmonella typhimurium carAB operons: CarP and integration host factor (IHF) modulate the methylation status of a GATC site present in the control region. J. Mol. Biol. 250:383-391.
13. Charlier, D., G. Hassanzadeh, A. Kholti, D. Gigot, A. Piérard, and N. Glansdorff. 1995. carP, involved in pyrimidine regulation of the Escherichia coli carbamoylphosphate synthetase operon encodes a sequence-specific DNA-binding protein identical to XerB and PepA. also required for resolution of ColE1 multimers. J. Mol. Biol. 250:392-406.
14. Devries, C. A., and D. E. Ohman. 1994. Mucoid-to-nonmucoid conversion in alginate-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT. encoding a putative alternative sigma factor, and shows evidence for autoregulation. J. Bacteriol. 176:6677-6687.
15. Erickson, J. W., and C. A. Gross. 1989. Identification of the subunit of Escherichia coli RNA polymerase: a second alternate sigma factor involved in high-temperature gene expression. Genes Dev. 3:1462-1471.
16. Fellay, R., J. Frey, and H. Krisch. 1987. Interposon mutagenesis of soil and water bacteria: a family of DNA fragments designed for in vitro insertional mutagenesis of Gram-negative bacteria. Gene 52:147-154.
17. Figurski, D., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. USA 76:1648-1652.
18. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J. F. Tomb, B. A. Dougherty, and J. M. E. A. Merrick. 1995. Whole-genome sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
19. Flynn, J. L., and D. E. Ohman. 1988. Cloning of genes from mucoid Pseudomonas aeruginosa which control spontaneous conversion to the alginate production phenotype. J. Bacteriol. 170;1452-1460.
20. Fürste, J. P., W. Pansegrau, R. Frank, H. Blöcker, P. Scholz, M. Bagdasarian, and E. Lanka. 1986. Molecular cloning of the plasmid RP4 primase region in a multi-host-range tacP expression vector. Gene 48:119-131.
21. Goff, S. A., and A. L. Goldberg. 1985. Production of abnormal proteins in Escherichia coli stimulates transcription of lon and other heat shock genes. Cell 41:587-595.
22. Goldberg, J. B., and T. Dahnke. 1992. Pseudomonas aeruginosa AlgB, which modulates the expression of alginate, is a member of the NtrC subclass of prokaryotic regulators. Mol. Microbiol. 6:59-66.
23. Goldberg, J. B., and D. E. Ohman. 1987. Construction and characterization of Pseudomonas aeruginosa algB mutants: role of algB in high-level production of alginate. J. Bacteriol. 169:1593-1602.
24. Govan, J. R. W., and V. Deretic. 1996. Microbial pathogenesis in cystic fibrosis: mucoid Pseudomonas aeruginosa and Burkholderia cepacia. Microbiol. Rev. 60:539-574.
25. Grunstein, M., and D. Hogness. 1975. Colony hybridization: a method for the isolation of cloned DNAs that contain a specific gene. Proc. Natl. Acad. Sci. USA 72:3961.
26. Hauser, A. R., P. J. Kang, and J. N. Engel. 1998. PepA, a secreted protein of Pseudomonas aeruginosa, is necessary for cytotoxicity and virulence. Mol. Microbiol. 27:807-818.
27. Hermes, H. F. M., T. Sonke, P. J. H. Peters, J. A. M. van Balken, J. Kamphuis, L. Dijkhuizen, and E. M. Meijer. 1993. Purification and characterization of an L-aminopeptidase from Pseudomonas putida ATCC 12633. Appl. Environ. Microbiol. 59:4330-4334.
28. Holloway, B. W. 1969. Genetics of Pseudomonas. Bacteriol. Rev. 33:419-443.
29. Holloway, B. W. 1996. Pseudomonas genetics and taxonomy, p. 22-32. In T. Nakazawa, K. Furukawa, D. Haas, and S. Silver (ed.). Molecular biology of pseudomonads. ASM Press, Washington, D.C.
30. 32 in Escherichia coli. J. Bacteriol. 176:5648-5653.
31. Ma, S., U. Selvaraj, D. E. Ohman, R. Quarless, D. J. Hassett, and D. J. Wozniak. 1998. Phosphorylation-independent activity of the response regulators AlgB and AlgR in promoting alginate biosynthesis in mucoid Pseudomonas aeruginosu. J. Bacteriol. 180:956-968.
32. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Martin, D. W., B. W. Holloway, and V. Deretic. 1993. Characterization of a locus determining the mucoid status of Pseudomonas aeruginosa: AlgU shows sequence similarities with a Bacillus sigma factor. J. Bacteriol. 175: 1153-1164.
34. Martin, D. W., M. J. Schurr, M. H. Mudd, J. R. W. Govan, B. W. Holloway, and V. Deretic. 1993. Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients. Proc. Natl. Acad. Sci. USA 90: 8377-8381.
35. e and stress response. J. Bacteriol. 176:6688-6696.
36. McCulloch, R., M. E. Burke, and D. J. Sherratt. 1994. Peptidase activity of Escherichia coli aminopeptidase A is not required for its role in Xer site-specific recombination. Mol. Microbiol. 12:241-251.
37. Ohman, D. E., and A. M. Chakrabarty. 1981. Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate. Infect. Immun. 33:142-148.
38. Prentki, P., and H. M. Kirsch. 1984. In vitro insertional mutagenesis with a selectable DNA fragment. Gene 29:303-313.
39. Schmidt, K. D., B. Tummler, and U. Romling. 1996 Comparative genome mapping of Pseudomonas aeruginosa PAO with P. aeruginosa C, which belongs to a major clone in cystic fibrosis patients and aquatic habitats. J. Bacteriol. 178:85-93.
40. E-like family of stress sigma factors, by the negative regulators MucA and MucB and Pseudomonas aeruginosa conversion to mucoidy in cystic fibrosis. J. Bacteriol. 178:4997-5004.
41. Schweizer, H. P., and T. T. Hoang. 1995. An improved system for gene replacement and xy/E fusion analysis in Pseudomonas aeruginosa. Gene 158: 15-22.
42. Simon, R., J. Quandt, and W. Klipp. 1989. New derivatives of transposon Tn5 suitable for mobilization of replicons, generation of operon fusions and induction of genes in Gram-negative bacteria. Gene 80:161-169.
43. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
44. Staskawicz, B., D. Dahlbeck, N. Keen, and C. Napoli. 1987. Molecular characterization of cloned avirulence genes from race-0 and race-1 of Pseudomonas syringae pv. glycinea. J. Bacteriol. 169:5789-5794.
45. Stirling, C. J., S. D. Colloms, J. F. Collins, G. Szatmari, and D. J. Sherratt. 1989. xerB, an Escherichia coli gene required for plasmid ColE1 site-specific recombination, is identical to pepA, encoding aminopeptidase A, a protein with substantial similarity to bovine lens leucine aminopeptidase. EMBO J. 8:1623-1627.
46. Stock, J. B., M. G. Surette, M. Levit, and P. Park. 1995. Two-component signal transaction systems: structure-function relationships and mechanisms of catalysis, p. 25-51. In J. A. Hoch, and T. J. Silhavy (ed.), Two-component signal transduction. ASM Press, Washington, D.C.
47. Terry, J. M., S. E. Pina, and S. J. Mattingly. 1991 Environmental conditions which influence mucoid conversion in Pseudomonas aeruginosa PAO1. Infect. Immun. 59:471-477.
48. Vieira, J., and J. Messing. 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259-268.
49. Vogt, V. M. 1970. Purification and properties of an aminopeptidase from Escherichia coli. J. Biol. Chem. 245:4760-4769.
50. Wozniak, D. J., and D. E. Ohman. 1991. Pseudomonas aeruginosa AlgB. a two-component response regulator of the NtrC family, is required for algD transcription. J. Bacteriol. 173:1406-1413.
51. Wozniak, D. J., and D. E. Ohman. 1994. Transcriptional analysis of the Pseudomonas aeruginosa genes algR, algB. and algD reveals a hierarchy of alginate gene expression which is modulated by algT. J. Bacteriol. 176:6007-6014.
52. Xie, Z., D. Hershberger, S. Shankar, R. W. Ye, and A. M. Chakrabarty. 1996. Sigma factor-anti-sigma factor interaction in alginate synthesis: inhibition of AlgT by MucA. J. Bacteriol. 178:4990-4996.
53. Yanisch-Perron, C., J. Vieira, and J. Messing. 1985. Improved M13 cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103-119.
54. Zhang, X., L. Zhu, and M. P. Deutscher. 1998. Oligoribonuclease is encoded by a highly conserved gene in the 3′-5′ exonuclease superfamily. J. Bacteriol. 180:2779-2781.