Sigma factor-anti-sigma factor interaction in alginate synthesis: Inhibition of AlgT by MucA

Journal of Bacteriology

Volumn 178, Issue 16, 1996, Pages 4990-4996

  Xie, Zhi Dong ^a   Hershberger, C Douglas ^a   Shankar, Sandeep ^a   Ye, Rick W ^a,b   Chakrabarty, A M ^a  

^a UNIVERSITY OF ILLINOIS   (United States)

^b DUPONT COMPANY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; SIGMA FACTOR;

ANTIGEN ANTIBODY COMPLEX; ARTICLE; BACTERIAL GENE; BACTERIAL METABOLISM; ENZYME LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0029783759     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.178.16.4990-4996.1996     Document Type: Article

References (29)

1. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.). 1993. Current protocols in molecular biology, vol. 1, p. 2.4.1-2.4.2. John Wiley & Sons, New York
2. B is regulated by a binding protein (RsbW) that blocks its association with core RNA polymerase. Proc. Natl. Acad. Sci. USA 90:2330-2334.
3. Bowman, B. J., F. Blasco, and S. W. Slayman. 1981. Purification and characterization of the plasma membrane ATPase of Neurospora crassa. J. Biol. Chem. 256:12343-12349.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Brown, K. L. and K. T. Hughes. 1995. The role of anti-sigma factors in gene regulation. Mol. Microbiol. 16:397-404.
6. Collins, F. S. 1992. Cystic fibrosis: molecular biology and therapeutic implications. Science 256:774-779.
7. Deretic, V., M. J. Schurr, J. C. Boucher, and D. W. Martin. 1994. Conversion of Pseudomonas aeruginosa to mucoidy in cystic fibrosis: environmental stress and regulation of bacterial virulence by alternative sigma factors. J. Bacteriol. 176:2773-2780.
8. DeVries, C. A., and D. E. Ohman. 1994. Mucoid-to-nonmucoid conversion in alginatc-producing Pseudomonas aeruginosa often results from spontaneous mutations in algT, encoding a putative alternate sigma factor, and shows evidence for autoregulation. J. Bacteriol. 176:6677-6687.
9. F from Bacillus subtilis. Proc. Natl. Acad. Sci. USA 90:2325-2329.
10. Evans, L. R., and A. Linker. 1973. Production and characterization of the slime polysaccharide of Pseudomonus aeruginosa. J. Bacteriol. 116:915-924.
11. Flynn, J. L., and D. E. Ohman. 1988. Cloning of genes from mucoid Pseudomonas aeruginosa which control spontaneous conversion to the alginate production phenotype. J. Bacteriol. 170:1452-1460.
12. Flynn, J. L., and D. E. Ohman. 1988. Use of a gene replacement cosmid vector for cloning alginate conversion genes from mucoid and nonmucoid Pseudomonas aeruginosa strains: algS controls expression of algT. J. Bacteriol. 170:3228-3236.
13. Fyfe, J. A. M., and J. R. W. Govan. 1980. Alginatc synthesis in mucoid Pseudomonas aeruginosa: a chromosomal locus involved in control. J. Gen. Microbiol. 119:443-450.
14. Goldberg, J. B., W. L. Gorman, J. L. Flynn, and D. E. Ohman. 1993. A mutation in algN permits trans activation of alginate production by algT in Pseudomonas species. J. Bacteriol. 175:1303-1308.
15. E). Proc. Natl. Acad. Sci. USA 91:7941-7945.
16. Liu, X., and P. Matsumura. 1995. An alternative sigma factor controls transcription of flagellar class-III operons in Escherichia coli - gene sequence, overproduction, purification and characterization. Gene 164:81-84.
17. Lonetto, M. A., K. L. Brown, K. E. Rudd, and M. J. Buttner. 1994. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase σ factors involved in the regulation of extracytoplasmic functions. Proc. Natl. Acad. Sci. USA 91:7573-7577.
18. MacGeorge, J., V. Korolik, A. F. Morgan, V. Asche, and B. W. Holloway. 1986. Transfer of a chromosomal locus responsible for mucoid colony morphology in Pseudomonas aeruginosa isolated from cystic fibrosis patients to P. aeruginosa PAO. J. Med. Microbiol. 21:331-336.
19. Martin, D. E., B. W. Holloway, and V. Deretic. 1993. Characterization of a locus determining the mucoid status of Pseudomonas aeruginosa: AlgU shows sequence similarities with a Bacillus sigma factor. J. Bacteriol. 175: 1153-1164.
20. Martin, D. W., M. J. Schurr, M. H. Mudd, and V. Deretic. 1993. Differentiation of Pseudomonas aeruginosa into the alginate-producing form: inactivation of mucB causes conversion to mucoidy. Mol. Microbiol. 9:497-506.
21. Martin, D. W., M. J. Schurr, M. H. Mudd, J. R. W. Govan, B. W. Holloway, and V. Deretic. 1993. Mechanism of conversion to mucoidy in Pseudomonas aeruginosa infecting cystic fibrosis patients. Proc. Natl. Acad. Sci. USA 90: 8377-8381.
22. May, T. B., and A. M. Chakrabarty. 1994. Pseudomonas aeruginosa: genes and enzymes of alginate synthesis. Trends Microbiol. 2:151-157.
23. Ohman, D. E., and A. M. Chakrabarty. 1981. Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate. Infect. Immun. 33:142-148.
24. Ohman, D. E., and J. B. Goldberg. 1990. Genetics of alginate biosynthesis in Pseudomonas aeruginosa, p. 206-220. In P. Gacesa and N. J. Russell (ed.), Pseudomonas infection and alginates: biochemistry, genetics, and pathology. Chapman and Hall, London.
25. Schurr, M. J., D. W. Martin, M. H. Mudd, and V. Deretic. 1994. Gene cluster controlling conversion to alginate-overproducing phenotype in Pseudomonas aeruginosa: functional analysis in a heterologous host and role in the instability of mucoidy. J. Bacteriol. 176:3375-3382.
26. Schurr, M. J., J. M. Martinez-Salazar, H. Yu, N. S. Hibler, and V. Deretic. 1995. Biochemical characterization and posttranslational modification of AlgU, a regulator of stress response in Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 216:874-880.
27. Shukla, D., and P. Matsumura. 1995. Mutations leading to altered CheA binding cluster on a face of CheY. J. Biol. Chem. 270:24414-24419.
28. Sundin, G. W., S. Shankar, S. A. Chugani, B. A. Chopade, A. Kavanaugh-Black, and A. M. Chakrabarty. 1996. Nucleosidc diphosphate kinase from Pseudomonas aeruginosa: characterization of the gene and its role in cellular growth and exopolysaccharidc alginate synthesis. Mol. Microbiol. 20:965-979.
29. E and Pseudomonas aeruginosa AlgU: E. coli rpoE restores mucoidy and reduces sensitivity to reactive oxygen intermediates in algU mutants of P. aeruginosa. J. Bacteriol. 177:3259-3268.